Download or read book Microscopy Optical Spectroscopy and Macroscopic Techniques written by Christopher Jones and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.
Download or read book Methods in Molecular Biology Microscopy optical spectroscopy and macroscopic techniques written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optical Spectroscopic and Microscopic Techniques written by Harekrushna Sahoo and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.
Download or read book Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications written by Vicente Micó and published by Frontiers Media SA. This book was released on 2021-10-22 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book New Techniques of Optical Microscopy and Microspectroscopy written by Cherry and published by CRC Press. This book was released on 1991 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The advent of new techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutioniz-ing the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sen-sor molecules for cellular assays. The aim of this volume will be to bring together the various advances in order to provide the reader with an up-to-date account of what can now be achieved with modern optical microscopic methods.
Download or read book A Practical Guide to Optical Microscopy written by John Girkin and published by CRC Press. This book was released on 2019-06-14 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.
Download or read book Optical Spectroscopic and Microscopic Techniques written by Harekrushna Sahoo and published by Springer Nature. This book was released on 2022-02-25 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.
Download or read book Microscopic and Spectroscopic Imaging of the Chemical State written by Michael D. Morris and published by CRC Press. This book was released on 1993-07-26 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents chemical state imaging methods useful on distance scales ranging from individual atoms to millimeters. This work is intended for chemists familiar with modern spectroscopies, but includes tutorial material on basic imaging processes for those with little background in the field.
Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer Nature. This book was released on 2020-03-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.
Download or read book Microscopic Methods in Metals written by Ulrich Gonser and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of scientific investigation can be divided into two categories: they are either macroscopic or microscopic in nature. The former are generally older, classical methods where the sample as a whole is studied and various local prop erties are deduced by differentiation. The microscopic methods, on the other hand, have been discovered and developed more recently, and they operate for the most part on an atomistic scale. Glancing through the shelves of books on the various scientific fields, and, in particular, on the field of physical metallurgy, we are surprised at how lit tle consideration has been given to the microscopic methods. How these tools provide new insight and information is a question which so far has not at tracted much attention. Similar observations can be made at scientific confer ences, where the presentation of papers involving microscopic methods is often pushed into a far corner. This has led users of such methods to organize their own special conferences. The aim of this book is to bridge the present gap and encourage more interaction between the various fields of study and selected microscopic meth ods, with special emphasis on their suitability for investigating metals. In each case the principles of the method are reviewed, the advantages and successes pointed out, but also the shortcomings and limitations indicated.
Download or read book Microscopy Techniques written by Jens Rietdorf and published by Springer Science & Business Media. This book was released on 2005-06-23 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts
Download or read book Near field Coherent Optical Spectroscopy and Microscopy of a Mesoscopic Quantum System written by Jeffrey Randall Guest and published by . This book was released on 2000 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optics and Spectroscopy at Surfaces and Interfaces written by Vladimir G. Bordo and published by Wiley-VCH. This book was released on 2005 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers linear and nonlinear optics as well as optical spectroscopy at solid surfaces and at interfaces between a sold and a liquid or gas. In the first part, the author gives a concise introduction to the physics of surfaces and interfaces. They discuss in detail physical properties of solid surfaces and of their interfaces to liquid and gases. The necessary theoretical background for understanding various optical techniques is provided thereafter. The second part of the book gives a broad review on optical techniques and their applications such as infrared and optical spectroscopy or optical microscopy. Discussions of nonlinear optics, but also nano-optics and local spectroscopies complement this self-contained work. Helpful features includes problems with solutions, a glossary, an index and a thoroughly elaborated list of topical references. The book is suited as a text of graduate students but also for scientists working in physics, chemistry, materials or life sciences who look for an expert introduction to surface optical aspects of their studies.
Download or read book Biomedical Optical Imaging written by James G. Fujimoto and published by Oxford University Press. This book was released on 2009-04-22 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.
Download or read book Microscopic Techniques for the Non Expert written by Sathish-Kumar Kamaraj and published by Springer Nature. This book was released on 2022-06-27 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers fundamental microscopic techniques for Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), and other microscopic tools. It provides step-by-step instructions and explanations of the basic fundamental concepts and mechanisms and guides the reader on resolving queries related to taking and analyzing microscopy images. The latest advancements and developments in microscopic equipment are described. Theoretical background on microscopy is also provided to enhance the reader’s understanding of microscopy techniques and tools. Microscopic Techniques for the Non-Expert is an ideal book for undergraduate and postgraduate students, as well as researchers with a background in environmental science, materials science, biomedicine, engineering, or bio-nanotechnology.
Download or read book Optical Techniques in Biological Research written by Denis L. Rousseau and published by . This book was released on 1984 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Light scattering / Bennie R. Ware -- Raman scattering / Denis L. Rousseau and Mark R. Ondrias -- Fourier transform infrared spectroscopy / James O. Alben and Frank G. Fiamingo -- Nanosecond fluorescence spectroscopy / Juan Yguerabide and Evangelina E. Yguerabide -- Circular dichroism / Marcos F. Maestre -- Electron microscopy / S.W. Hui.
Download or read book Modern Optical Spectroscopy written by William W. Parson and published by Springer. This book was released on 2015-06-09 with total page 583 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook offers clear explanations of optical spectroscopic phenomena and shows how spectroscopic techniques are used in modern molecular and cellular biophysics and biochemistry. The topics covered include electronic and vibrational absorption, fluorescence, resonance energy transfer, exciton interactions, circular dichroism, coherence and dephasing, ultrafast pump-probe and photon-echo spectroscopy, single-molecule and fluorescence-correlation spectroscopy, Raman scattering, and multiphoton absorption. This revised and updated edition provides expanded discussions of quantum optics, metal-ligand charge-transfer transitions, entropy changes during photoexcitation, electron transfer from excited molecules, normal-mode calculations, vibrational Stark effects, studies of fast processes by resonance energy transfer in single molecules, and two-dimensional electronic and vibrational spectroscopy. The explanations are sufficiently thorough and detailed to be useful for researchers and graduate students and advanced undergraduates in chemistry, biochemistry and biophysics. They are based on time-dependent quantum mechanics, but are developed from first principles with a clarity that makes them accessible to readers with little prior training in this field. Extra topics and highlights are featured in special boxes throughout the text. The author also provides helpful exercises for each chapter.
