Download or read book Microsatellite Marker Development Characterization and Mapping in European Hazelnut Corylus Avellana L and Investigation of Novel Sources of Eastern Filbert Blight Resistance in Corylus written by Gehendra Bhattarai and published by . This book was released on 2015 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: European hazelnut, an important nut crop in Oregon agriculture, is threatened by the fungal disease eastern filbert blight (EFB) caused by Anisogramma anomala (Peck) E. Müller. The hazelnut breeding program at OSU has been working on development of EFB-resistant cultivars. DNA markers allow mapping of traits using segregating progenies and identification of linked markers. The breeding of hazelnuts involves use of DNA markers to genotype individuals and facilitate selection in a procedure known as marker-assisted selection (MAS). This technique is particularly promising in tree crops with a long juvenile phase like the hazelnuts. 'Gasaway' resistance governed by a dominant allele at a single locus was identified and several EFB-resistant cultivars carrying the dominant allele from this source have been released. Infection of 'Gasaway' and some of its offspring by A. anomala isolates has been noted, and indicates an urgent need to investigate new sources of resistance, identify new resistance genes and incorporate them into the breeding effort. New microsatellite markers were developed from the genomic sequence of hazelnut cv. 'Jefferson'. A total of 17,802 simple sequence repeat (SSR) motifs exceeding 15 bp in length and consisting of tri-, tetra-, penta- and hexa- nucleotide repeats were identified in the 'Jefferson' genome. Removal of duplicates, short fragments, repeats at ends, and repeats containing only A's and T's reduced the number of unique fragments to 2,069. Visual inspection of these unique fragments alligned with genome sequences of 7 other accessions in Tablet software identified 489 sequences with variation in number of repeats but with conserved flanking regions. Amplification of a set of 24 diverse accessions and separation on agarose gels led to the development of 366 new polymorphic markers. These were used to genotype 48 diverse accessions using capillary electrophoresis for fragment sizing. An additional 15 parents were also fingerprinted at these new 366 microsatellite loci. Using the mapping population (OSU 252.146 x OSU 414.062), 213 loci were mapped. A dendrogram was constructed which showed a high level of polymorphism in hazelnut. Tightly linked markers were identified for 'Gasaway' resistance and MAS is routinely used. The hazelnut acreage in Oregon is increasing every year with the planting of these new EFB-resistant cultivars. However, concern about disease resistance breakdown prompted an investigation of 12 new EFB resistance sources. Segregating progenies were inoculated by placement of potted trees under an inoculation structure, in the greenhouse, or in the field by tying a diseased branch on each tree. A chi-square goodness-of-fit test was used compare observed with expected segregation ratios. Very few seedlings of Moscow selections N01, N01-06, and N01-07 were resistant and were likely escapes. 'Grand Traverse', C. heterophylla 'Ogyoo', 'Yoder #5', C. americana 'Rush', Moscow N23, N26, N27, and N37 segregated in 1:1 ratio, indicating resistance governed by a single locus and a dominant allele for resistance. 'Uebov' transmitted resistance to only ~15 % of its seedlings. Resistance from 'Uebov', 'Grand Traverse', and C. heterophylla 'Ogyoo' were assigned to LG6 but only 'Uebov' and 'Grand Traverse' were mapped. Resistance from 'Yoder #5', Moscow N27 and C. americana 'Rush' was assigned to LG7. Mapping of the remaining resistance sources will be completed in the future.
Download or read book DNA Markers and Characterization of Novel Sources of Eastern Filbert Blight Resistance in European Hazelnut Corylus Avellana L written by Brooke C. Peterschmidt and published by . This book was released on 2013 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: European hazelnut is a significant crop in the Pacific Northwest, and the US ranks 4th internationally for hazelnut production. Production in the Pacific Northwest is threatened, however, by the disease eastern filbert blight (EFB) caused by the fungus Anisogramma anomala (Peck) E. Müller. To meet the challenges faced by the hazelnut industry in Oregon and Washington, the breeding program at Oregon State University has focused on developing DNA marker technology and producing EFB resistant cultivars. This study focused on developing new microsatellite markers from hazelnut transcriptome sequences and on disease resistance from three accessions ('Culpla, ' 'Crvenje, ' and OSU 495.072) which showed no disease symptoms following a series of inoculations. DNA markers have been useful in hazelnut breeding for marker-assisted selection, construction of genetic linkage maps, cultivar fingerprinting, and phylogeny studies. Previously developed markers include AFLP, RAPD, ISSR, and microsatellite (SSR) markers developed from enriched libraries and ISSR fragments. This study utilized the transcriptome sequence from 'Jefferson' hazelnut to mine for microsatellites, align with the genomic sequence, design primers, screen for polymorphism, and characterize and map polymorphic markers. A total of 1432 microsatellites were mined from the transcriptome sequence, and the most frequently found motifs were AG (35.8%), AT (13.3%), and AAG (12.7%), and 382 primer pairs were designed. Screening showed that 119 markers were polymorphic, and these were characterized on sets of 50 and 14 accessions. Fifty-three markers that segregated in the mapping population or in three alternate populations were mapped and assigned to linkage groups. A dendrogram showed that accessions clustered mostly according to geographic origin. These results confirm the high level of diversity present in hazelnut, and the markers developed in this study will be useful for further genetics studies in hazelnut. The three EFB resistant parents 'Culpla, ' 'Crvenje, ' and OSU 495.072 were subjected to two inoculation treatments: greenhouse inoculations and exposure under an inoculation structure. The accessions remained free of disease after both treatments. Progeny segregating for resistance were produced. The progeny were inoculated either in the greenhouse or under the structure, and disease response recorded for each individual. DNA was extracted from seedlings, and sets of 32 seedlings from each resistant parent were screened with previously mapped markers using PCR and capillary electrophoresis. All three resistance sources were correlated with marker A614, allowing the resistance loci to be assigned to linkage group (LG) 6. The progeny were then screened with all known microsatellite markers on LG 6, and linkage maps constructed of the marker loci and resistance loci. Markers KG821, LG628, and LG696 are especially close to the resistance loci and will be useful for marker-assisted selection. Although these resistance loci are located in the same region of LG 6 as the 'Gasaway' resistance gene, they are different from 'Gasaway, ' and markers linked to resistance will be useful for introgressing and pyramiding resistance in new cultivars.
Download or read book Plant Breeding Reviews Volume 45 written by Irwin Goldman and published by John Wiley & Sons. This book was released on 2021-10-21 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Breeding Reviews presents state-of-the-art reviews on plant genetics and the breeding of all types of crops by both traditional means and molecular methods. Many of the crops widely grown today stem from a very narrow genetic base; understanding and preserving crop genetic resources is vital to the security of food systems worldwide. The emphasis of the series is on methodology, a fundamental understanding of crop genetics, and applications to major crops.
Download or read book Recent advances in hazelnut corylus spp written by Valerio Cristofori and published by Frontiers Media SA. This book was released on 2023-02-08 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advances in Plant Breeding Strategies Nut and Beverage Crops written by Jameel M. Al-Khayri and published by Springer Nature. This book was released on 2020-01-01 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the development of innovative modern methodologies towards augmenting conventional plant breeding, in individual crops, for the production of new crop varieties under the increasingly limiting environmental and cultivation factors to achieve sustainable agricultural production, enhanced food security, in addition to providing raw materials for innovative industrial products and pharmaceuticals. This Volume 4, subtitled Nut and Beverage Crops, focuses on advances in breeding strategies using both traditional and modern approaches for the improvement of individual plantation crops. Included in Part I, eleven important nut species recognized for their economical and nutritional importance including Almond, Argan, Brazil nut, Cashew nut, Chestnut, Hazelnut, Macadamia, Peanut, Pine nut, Pistachio and Walnut. Part II covers two popular beverage species, coffee and tea. This volume is contributed by 53 internationally reputable scientists from 13 countries. Each chapter comprehensively reviews the modern literature on the subject and reflects the authors own experience.
Download or read book Wild Crop Relatives Genomic and Breeding Resources written by Chittaranjan Kole and published by Springer Science & Business Media. This book was released on 2011-08-27 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wild crop relatives are now playing a significant part in the elucidation and improvement of the genomes of their cultivated counterparts. This work includes comprehensive examinations of the status, origin, distribution, morphology, cytology, genetic diversity and available genetic and genomic resources of numerous wild crop relatives, as well as of their evolution and phylogenetic relationship. Further topics include their role as model plants, genetic erosion and conservation efforts, and their domestication for the purposes of bioenergy, phytomedicines, nutraceuticals and phytoremediation. Wild Crop Relatives: Genomic and Breeding Resources comprises 10 volumes on Cereals, Millets and Grasses, Oilseeds, Legume Crops and Forages, Vegetables, Temperate Fruits, Tropical and Subtropical Fruits, Industrial Crops, Plantation and Ornamental Crops, and Forest Trees. It contains 125 chapters written by nearly 400 well-known authors from about 40 countries.
Download or read book Molecular Identification of Fungi written by Youssuf Gherbawy and published by Springer Science & Business Media. This book was released on 2010-03-03 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungi enjoy great popularity in pharmaceutical, agricultural, and biotechnological applications. Recent advances in the decipherment of whole fungal genomes promise an acceleration of these trends. This timely book links scientists from different parts of the world who are interested in the molecular identification of fungi combined with the exploration of the fungal biodiversity in different ecosystems. It provides a compendium for scientists who rely on a rapid and reliable detection of fungal specimens in environmental as well as clinical resources in order to ensure the benefit of industrial and clinical applications. Chapters focus on the opportunities and limits of the molecular marker-mediated identification of fungi. Various methods, procedures and strategies are outlined. Furthermore, the book offers an update of the current progress in the development of fungal molecular techniques, and draws attention to potential and associated problems, as well as integrating theory and practice.
Download or read book Ornamental Crops written by Johan Van Huylenbroeck and published by Springer. This book was released on 2018-07-25 with total page 887 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ornamental plants are economically important worldwide. Both growers and consumers ask continuously for new, improved varieties. Although there are numerous ornamental species, ornamental plant breeding and plant breeding research is mainly limited to some major species. This book focuses on the recent advances and achievements in ornamental plant breeding. The first part of the book focuses on plant traits and breeding techniques that are typical for ornamental plants. Eminent research groups write these general chapters. For plant traits like flower colour or shape, breeding for disease resistance and vase or shelf life are reviewed. General technical plant breeding chapters deal with mutation breeding, polyploidisation, in vitro breeding techniques and new developments in molecular techniques. The second part of the book consists of crop-specific chapters. Here all economically major ornamental species are handled together with selected representative species from different plant groups (cut flowers, pot plants, woody ornamental plants). In these crop-specific chapters, the main focus is on recent scientific achievements over the last decade.
Download or read book Descriptors for Hazelnut Corylus avellana L written by and published by Bioversity International. This book was released on 2008 with total page 64 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protocol for Somatic Embryogenesis in Woody Plants written by Shri Mohan Jain and published by Springer Science & Business Media. This book was released on 2005-05-23 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.
Download or read book Plant Tissue Culture Development and Biotechnology written by Robert N. Trigiano and published by CRC Press. This book was released on 2016-03-30 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
Download or read book Edible Ectomycorrhizal Mushrooms written by Alessandra Zambonelli and published by Springer Science & Business Media. This book was released on 2013-01-30 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edible ectomycorrhizal mushrooms (EEMMs) comprise more than 1000 species and are an important food and forest resource. In this volume of Soil Biology, internationally recognized scientists offer their most recent research findings on these beguiling fungi. Topics covered include: complex ecological interactions between plants, EEMMs, and soil organisms; comparative genomics, high-throughput sequencing and modern research tools; genetic selection of fungal strains and techniques for inoculating plants; economic and social considerations surrounding wild collected EEMMs; and practical information concerning soil management and EEMM cultivation. The book will be a useful guide for anyone interested in soil ecology, forestry, or the genetics and cultivation of EEMMs, and provides an extensive knowledge base and inspirations for future studies on these ecologically and economically important fungi.
Download or read book The Handbook of Plant Genome Mapping written by Khalid Meksem and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: While the complete sequencing of the genomes of model organisms such as a multitude of bacteria and archaea, the yeast Saccharomyces cerevisiae, the worm Caenorhabditis elegans, the fly Drosophila melanogaster, and the mouse and human genomes have received much public attention, the deciphering of plant genomeswas greatly lagging behind. Up to now, only two plant genomes, one of the model plant Arabidopsis thaliana and one of the crop species rice (Oryza sativa) have been sequenced, though a series of other crop genome sequencing projects are underway. Notwithstanding this public bias towards genomics of animals and humans, it is nevertheless of great importance for basic and applied sciences and industries in such diverse fields as agriculture, breeding in particular, evolutionary genetics, biotechnology, and food science to know the composition of crop plant genomes in detail. It is equally crucial for a deeper understanding of the molecular basis of biodiversity and synteny. The Handbook of Genome Mapping: Genetic and Physical Mapping is the first book on the market to cover these hot topics in considerable detail, and is set apart by its combination of genetic and physical mapping. Throughout, each chapter begins with an easy-to-read introduction, also making the book the first reference designed for non-specialists and newcomers, too. In addition to being an outstanding bench work reference, the book is an excellent textbook for learning and teaching genomics, in particular for courses on genome mapping. It also serves as an up-to-date guide for seasoned researchers involved in the genetic and physical mapping of genomes, especially plant genomes.
Download or read book Taming the Truffle written by Ian Robert Hall and published by Timber Press (OR). This book was released on 2007 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whether the world's best truffles are found in Piedmont or Perigord inspires impassioned debate, but the effects of dwindling supply and insatiable demand for the elusive, ultimate mushroom are unquestionable: prices through the roof, intrigue and deception, and ever more intensive efforts to cultivate. The secrets of when, how, and where to collect truffles have benn passed from generation to generation since ancient times, but artificial cultivation remains the holy grail. Here in the most comprehensive practical treatment of the gastronomic treasure to date, the art and science of the high-stakes pursuit come together. Their enthusiasm and expertise leavened with wry humor, the authors explore the newest techniques; they describe the commercial species in detail along with their host plants, natural habitats, cultivation and mintenance, pests and diseases, and harvesting with pigs, dogs, truffle flies, and even the electronic nose. Pursuit of the fungus that costs more than gold is not for the faint of heart nor for those in a hurry, as under ideal conditions, truffle production in artificial truffieres can begin after three years but results may not be seen until a decade after planting, and maximum yields not for another decade still. So there is time to read and prepare, and no better source than this one.
Download or read book Advances in Plant Breeding Strategies Breeding Biotechnology and Molecular Tools written by Jameel M. Al-Khayri and published by Springer. This book was released on 2016-02-02 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basic concept of this book is to examine the use of innovative methods augmenting traditional plant breeding towards the development of new crop varieties under different environmental conditions to achieve sustainable food production. This book consists of two volumes: Volume 1 subtitled Breeding, Biotechnology and Molecular Tools and Volume 2 subtitled Agronomic, Abiotic and Biotic Stress Traits. This is Volume 1 which consists of 21 chapters covering domestication and germplasm utilization, conventional breeding techniques and the role of biotechnology. In addition to various biotechnological applications in plant breeding, it includes functional genomics, mutations and methods of detection, and molecular markers. In vitro techniques and their applications in plant breeding are discussed with an emphasis on embryo rescue, somatic cell hybridization and somaclonal variation. Other chapters cover haploid breeding, transgenics, cryogenics and bioinformatics.
Download or read book Plant Genetic Resource Management written by B. S. Dhillon and published by Alpha Science International, Limited. This book was released on 2004-01-01 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diversity of the plant genetic resources plays a key role in agricultural development. Systematic identification, characterization, and evaluation of this diversity are a pre- requisite for its scientific management.
Download or read book Tree crop Interactions written by C. K. Ong and published by Oxford University Press, USA. This book was released on 1996 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: A framework for quantifying the various effects of tree-crop interactions. Mexed cropping of annuals and woody perennials: an analytical approach to productivity and management. Mulch and shade model for optimum alley-cropping design depending on soil fertility. Principles of resource capture and utilization of light and water. Microclimatic modifications in agroforestry. The water balance of mixed tree-cropi systems. Biological factors affecting form and function in woody-non-woody plant mixtures. Tree-soil-crop interactions on slopes. Root distribution of tress and cropos: competition and/or complementarity. Woody-non-woody plant mixtures: some afterhoughts.
