Download or read book Micromethods in Molecular Biology written by Volker Neuhoff and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based on practical experience and is therefore written as a prac tical manual. The fore-runners of the book were the manuals of the first and second EMBO-Courses on "Micromethods in Molecular Biology" which were held in G6ttingen in the spring of 1970 and the autumn of 1971. This book may serve as a manual not only for the participants of the third EMBO-Course to be held in G6ttingen in autumn 1973, but also for all experimenters who are interested in using micromethods. It must be emphasized from the outset that this book is conceived as a "cook book" and not as a monograph which attempts to revue the literature on micromethods critically. The methods described here in detail are performed routinely in the authors' laboratories and include all the practical details necessary for the successful appli cation of the micromethods. There are many other sensitive and excellent micro methods which are not included in this book, because the authors feel that in a "cook book" only methods for which they have personal experience and profi ciency should be described. Some readers may feel that the title promises more than the present contents of this book; however, if sufficient interest is shown in this volume, it may be possible to remedy such deficiencies in future editions.
Download or read book Micromethods in Molecular Biology written by Volker Neuhoff and published by . This book was released on 1973 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Micromethods in Molecular Biology written by Volker Neuhoff and published by Springer. This book was released on 2012-07-24 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based on practical experience and is therefore written as a prac tical manual. The fore-runners of the book were the manuals of the first and second EMBO-Courses on "Micromethods in Molecular Biology" which were held in G6ttingen in the spring of 1970 and the autumn of 1971. This book may serve as a manual not only for the participants of the third EMBO-Course to be held in G6ttingen in autumn 1973, but also for all experimenters who are interested in using micromethods. It must be emphasized from the outset that this book is conceived as a "cook book" and not as a monograph which attempts to revue the literature on micromethods critically. The methods described here in detail are performed routinely in the authors' laboratories and include all the practical details necessary for the successful appli cation of the micromethods. There are many other sensitive and excellent micro methods which are not included in this book, because the authors feel that in a "cook book" only methods for which they have personal experience and profi ciency should be described. Some readers may feel that the title promises more than the present contents of this book; however, if sufficient interest is shown in this volume, it may be possible to remedy such deficiencies in future editions.
Download or read book Practical Methods in Molecular Biology written by Robert F. Schleif and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume has evolved from a laboratory methods book that one of us first compiled nearly fifteen years ago. Since that time the book has undergone many minor revisions in order to include new methods and updated versions of older methods. The result has been an increasingly useful and more widely circulated book. However, the recent series of technological explosions generally lumped together under the name of "recombinant DNA technology" has been a turning point in the evolution of this previously underground publication. Minor revisions will no longer do. To keep the book useful we have had to make major revi sions and additions. The result is a dramatically expanded book that should be more useful to more people. The larger size and wider usefulness of the book have made this more formal publication seem a reasonable step to take. One of the reasons that this volume should be useful to many people is that it includes only procedures that have been used repeatedly by us and that have proven highly reliable both to ourselves and to others in our laboratories.
Download or read book Basic Methods in Molecular Biology written by Leonard G. Davis and published by . This book was released on 1994 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edition emphasizes the decisions that need to be made to select one procedure over another in the everyday workings of a molecular biology laboratory. Trouble-shooting problems that may emerge when performing such methods are included and concise instructions are provided.
Download or read book Recombineering written by Christopher R. Reisch and published by Humana. This book was released on 2022-06-14 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field.
Download or read book Laboratory Methods in Enzymology DNA written by and published by Elsevier. This book was released on 2013-09-02 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA
Download or read book Laboratory Methods in Cell Biology written by and published by Academic Press. This book was released on 2012-12-31 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields
Download or read book Applications of Molecular Microbiological Methods written by Torben Lund Skovhus and published by Caister Academic Press Limited. This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Innovative, constructive, and continually evolving technologies are propelling microbiology into an exciting new era. This new era will witness the harnessing and control of complex microbial communities in a huge variety of applications in the industrial, medical, and environmental spheres. State-of-the-art tools are being developed and utilized to analyze the molecules that microorganisms possess and generate, including DNA, RNA, proteins, lipids, and cellular metabolites. This book, written by international experts in the field, presents emerging molecular methods that allow the diversity of a microbial community to be surveyed and its functions to be investigated. The first section provides examples of the application of molecular microbiological methods in various industrial applications. The second section deals with the identification of microorganisms in medical settings, while the third section presents case studies that use molecular methods to assess the structure and function of microbial communities in natural environments. The fourth part of the book describes in greater detail the methods and technologies featured in the preceding case studies, including metagenomics, stable isotope probing, fluorescence in situ hybridization, quantitative PCR, reverse transcription PCR, and single cell methods. These detailed descriptions will enable readers to evaluate the applicability of various tools for approaching questions and case studies of their own. This practical, authoritative, and up-to-date volume is a valuable resource for anyone applying or developing molecular methods in any area of microbiology and is a recommended acquisition for all microbiology laboratories.
Download or read book MicroRNA Detection and Target Identification written by Tamas Dalmay and published by Springer Nature. This book was released on 2023-01-23 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Download or read book Methods in Molecular Biology and Protein Chemistry written by Brenda D. Spangler and published by Wiley. This book was released on 2002-06-14 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ein praxisorientierter Leitfaden für Studenten im Hauptstudium. Vermittelt werden aktuelle Techniken der Molekularbiologie und Biochemie als fester Bestandteil eines Forschungsprojektes. Auf diese Weise kann der Lernende die Auswirkung der Gentechnik anhand der praktischen Anwendung von nassanalytischen biochemischen Verfahren erkennen. Mit Computer-Tutorials für Sequenzierung, Genomik und Molekülmodellierung. Mit Tipps und Hinweisen zur Sichtung der Primärliteratur, zu mündlichen Berichten, zur Protokollierung des Labortagebuchs und zum Verfassen von Berichten. Mit einer Liste von amerikanischen und europäischen kommerziellen Anbietern von Reagenzien. Verständlich und anschaulich geschrieben. Ein unverzichtbares Lehrbuch für fortgeschrittene Studenten und Doktoranden. "Methods in Molecular Biology" ist jedoch NICHT geeignet für aktive Forscher auf der Suche nach den neuesten hochdifferenzierten Verfahren.
Download or read book Handbook of Micromethods for the Biological Sciences written by Georg Keleti and published by Van Nostrand Reinhold Company. This book was released on 1973 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This handbook has 106 procedures each specifically detailing a biochemical or microbiological method of preparation or analysis. They are written in a concise, step-by-step form ... Reagents are specified ... Exact methods of preparation are delineated. Illustrations are provided where required to clarify a method." Pref. Indexed.
Download or read book Phase Separated Biomolecular Condensates written by Huan-Xiang Zhou and published by Springer Nature. This book was released on 2022-10-13 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with a broad collection of theoretical, computational, and experimental methods to quantitatively study the properties of phase-separate biomolecular condensates in diverse systems. The chapters in this book cover topics such as theoretical and computational methods; methods for in vitro characterization of biomolecular condensates; and techniques that enable in-cell characterization of biomolecular condensates. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Phase-Separated Biomolecular Condensates: Methods and Protocols is a valuable resource that helps researchers learn and use established methods to study both biophysical properties and biological functions of biomolecular condensates.
Download or read book Metagenomics written by Wolfgang R. Streit and published by Springer Nature. This book was released on 2022-10-28 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like function-driven mining of metagenomic DNA, screening and analyzing protocols for a wide array of different genes encoding enzymes, bacterial viruses and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.
Download or read book The Bacterial Nucleoid written by Olivier Espéli and published by Humana Press. This book was released on 2017-08-25 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents state-of-the-art protocols for key experiments that have revolutionized our understanding of the bacterial nucleoid. This book is divided into five parts: Part I introduces molecular genetic methods to study bacterial nucleoids; Part II highlights the study of bacterial nucleoid with whole genome analysis method; Part III discusses molecular biology methods to study nucleoid structuring factors; Part IV looks at imaging bacterial nucleoid; and Part V explores biophysics of the bacterial nucleoid. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, The Bacterial Nucleoid: Methods and Protocols is a valuable resource that provides a wealth of new information about this chromosome.
Download or read book Microinjection written by Chengyu Liu and published by Humana Press. This book was released on 2018-10-18 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.
Download or read book Plant Microtechniques and Protocols written by Edward Chee Tak Yeung and published by Springer. This book was released on 2015-09-19 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
