Download or read book Microfluidics enabled Combinatorial Chemistry for High throughput Screening written by Siwei Zhao and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Combinatorial chemistry is one of the most important technological advances in the last century in the field of biomedicine and material sciences. Allowing rapid synthesis and screening of a large number of compounds for certain functions or properties, combinatorial chemistry has greatly speeded up the discovery of new materials in the areas of cancer treatment and diagnosis, cell surface profiling, biomolecule epitope mapping, electronic/magnetic system, catalyst development, etc. Although current combinatorial chemistry methods have been capable of creating and screening very large-scale combinatorial libraries, the structural identification of positive hits is not straight forward and can be extremely time consuming. In this dissertation, we aim to develop a novel Microfluidics-enabled Combinatorial Chemistry method, which delivers large-scale peptide library, enables high-throughput screening and permits easy chemical identification as a package. Inspired by the contemporary matrix theories, a systemic approach has been developed to design and optimize a microfluidic network for parallel synthesis of exclusive large-scale combinatorial libraries. A facile multi-layer microfluidics fabrication strategy is implemented by the theoretical operations utilizing laser micromachining and reversible bonding techniques, which makes it widely applicable to regular biomedical and material discoveries. Moreover, a novel digitally encoded microdisc array platform is used as the substrate for combinatorial library synthesis, in which each carrier is assigned with a binary barcode and exhibits a unique chemical activity. This platform allows direct chemical identification of library compounds and thus leads to high-efficient and high-throughput combinatorial screening. We demonstrate the applicability of our Microfluidics-enabled Combinatorial Chemistry method by synthesizing and screening a combinatorial tetrapeptide library for discovery of cancer-targeting ligands. Peptide ligands against [alpha]4[beta]1 cancer cell surface integrin with high binding affinity have been identified. Therefore, we believe that our Microfluidics-enabled Combinatorial Chemistry approach can serve as a generic platform for broader applications of high-throughput biomolecule screening, including but not limited to peptidomimetics, small molecules, DNA/RNA and polysaccharides.
Download or read book R F A Newsletters Nos 11 to 20 Oct 1966 to June 1971 written by and published by . This book was released on 1971 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book High throughput Screening in Combinatorial Chemistry for Drug Discovery written by Ante M. Krstulović and published by . This book was released on 1999 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidic Devices for Biomedical Applications written by Xiujun (James) Li and published by Woodhead Publishing. This book was released on 2021-08-05 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidic Devices for Biomedical Applications, Second Edition provides updated coverage on the fundamentals of microfluidics, while also exploring a wide range of medical applications. Chapters review materials and methods, microfluidic actuation mechanisms, recent research on droplet microfluidics, applications in drug discovery and controlled-delivery, including micro needles, consider applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds, and cover the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. This book is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores a wide range of medical applications Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies Details applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and its role in developing tissue scaffolds, and stem cell engineering
Download or read book Microfluidics in Pharmaceutical Sciences written by Dimitrios A. Lamprou and published by Springer Nature. This book was released on with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book High Throughput Analysis in the Pharmaceutical Industry written by Perry G. Wang and published by CRC Press. This book was released on 2008-08-20 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of combinatorial chemistry technology has increased the amount of compounds generated in a year from 50 to 2000. Conventional analytical approaches simply cannot keep up. These circumstances have caused drug discovery to take on the shape of a bottleneck, like traffic through a toll booth. In order to break the bottleneck, a corres
Download or read book Encyclopedia of Microfluidics and Nanofluidics written by Dongqing Li and published by Springer Science & Business Media. This book was released on 2008-08-06 with total page 2242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering all aspects of transport phenomena on the nano- and micro-scale, this encyclopedia features over 750 entries in three alphabetically-arranged volumes including the most up-to-date research, insights, and applied techniques across all areas. Coverage includes electrical double-layers, optofluidics, DNC lab-on-a-chip, nanosensors, and more.
Download or read book Combinatorial Library written by Lisa B. English and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: The continued successes of large- and small-scale genome sequencing projects are increasing the number of genomic targets available for drug d- covery at an exponential rate. In addition, a better understanding of molecular mechanisms—such as apoptosis, signal transduction, telomere control of ch- mosomes, cytoskeletal development, modulation of stress-related proteins, and cell surface display of antigens by the major histocompatibility complex m- ecules—has improved the probability of identifying the most promising genomic targets to counteract disease. As a result, developing and optimizing lead candidates for these targets and rapidly moving them into clinical trials is now a critical juncture in pharmaceutical research. Recent advances in com- natorial library synthesis, purification, and analysis techniques are not only increasing the numbers of compounds that can be tested against each specific genomic target, but are also speeding and improving the overall processes of lead discovery and optimization. There are two main approaches to combinatorial library production: p- allel chemical synthesis and split-and-mix chemical synthesis. These approaches can utilize solid- or solution-based synthetic methods, alone or in combination, although the majority of combinatorial library synthesis is still done on solid support. In a parallel synthesis, all the products are assembled separately in their own reaction vessels or microtiter plates. The array of rows and columns enables researchers to organize the building blocks to be c- bined, and provides an easy way to identify compounds in a particular well.
Download or read book Macromolecular Crystallography Part C written by Charles W. Carter Jr. and published by Elsevier. This book was released on 2003-12-15 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Accurate molecular structures are vital for rational drug design and for structure-based functional studies directed toward the development of effective therapeutic agents and drugs. Crystallography can reliably predict structure, both in terms of folding and atomic details of bonding. * Methodological methods in crystals* Methodological methods data analysis
Download or read book A Practical Guide to Combinatorial Chemistry written by Anthony W. Czarnik and published by . This book was released on 1997 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combinatorial chemistry is the ability to simultaneously synthesize vast numbers of diverse compounds. Its techniques have revolutionized the drug discovery process, and are widely used throughout the biotechnology community. Aimed at a wide audience, this text is a down-to-earth introductionto small molecule combinatorial chemistry. It uses a tutorial approach to provide a detailed survey of solid-phase peptide synthesis and solution-phase synthesis. It also reviews current automated approaches and equipment for both solid- and solution-phase library synthesis.
Download or read book Droplet and Digital Microfluidics written by Sanket Goel and published by Elsevier. This book was released on 2024-03-11 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Droplet and Digital Microfluidics: Ideation to Implementation is a detailed introduction to the dynamics of droplet and digital microfluidics, also featuring coverage of new methods and applications. The explosion of applications of microelectromechanical systems (MEMS) in recent years has driven demand for expertise and innovation in fluid flow in the microchannels they contain. In this book, detailed descriptions of methods for biological and chemical applications of microfluidics are provided, along with supporting foundational knowledge. In addition, the principles of droplet and digital microfluidics are explained, along with their different applications and governing physics. New additions to the technological knowledgebase that enable advances in droplet and digital microfluidics include machine learning and exciting future avenues for research. Provides step-by-step fabrication, testing, and characterization instructions in each chapter to support implementation Includes explanations of applications and methods in biological and chemical settings Describes the path to automation of digital and droplet microfluidic platforms
Download or read book Combinatorial Materials Synthesis written by Xiao-Dong Xiang and published by CRC Press. This book was released on 2003-08-19 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pioneered by the pharmaceutical industry and adapted for the purposes of materials science and engineering, the combinatorial method is now widely considered a watershed in the accelerated discovery, development, and optimization of new materials. Combinatorial Materials Synthesis reveals the gears behind combinatorial materials chemistry and thin-film technology, and discusses the prime techniques involved in synthesis and property determination for experimentation with a variety of materials. Funneling historic innovations into one source, the book explores core approaches to synthesis and rapid characterization techniques for work with combinatorial materials libraries.
Download or read book Microfluidic Devices in Nanotechnology written by Challa S. S. R. Kumar and published by John Wiley & Sons. This book was released on 2010-11-29 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology, especially microfabrication, has been affecting every facet of traditional scientific disciplines. The first book on the application of microfluidic reactors in nanotechnology, Microfluidic Devices in Nanotechnology provides the fundamental aspects and potential applications of microfluidic devices, the physics of microfluids, specific methods of chemical synthesis of nanomaterials, and more. As the first book to discuss the unique properties and capabilities of these nanomaterials in the miniaturization of devices, this text serves as a one-stop resource for nanoscientists interested in microdevices.
Download or read book High troughput Screening in Combinatorial Chemistry for Drug Discovery written by Ante M. Krstulovic and published by . This book was released on 1999 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Encyclopedia of Analytical Science written by and published by Elsevier. This book was released on 2019-04-02 with total page 5142 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of the Encyclopedia of Analytical Science, Ten Volume Set is a definitive collection of articles covering the latest technologies in application areas such as medicine, environmental science, food science and geology. Meticulously organized, clearly written and fully interdisciplinary, the Encyclopedia of Analytical Science, Ten Volume Set provides foundational knowledge across the scope of modern analytical chemistry, linking fundamental topics with the latest methodologies. Articles will cover three broad areas: analytical techniques (e.g., mass spectrometry, liquid chromatography, atomic spectrometry); areas of application (e.g., forensic, environmental and clinical); and analytes (e.g., arsenic, nucleic acids and polycyclic aromatic hydrocarbons), providing a one-stop resource for analytical scientists. Offers readers a one-stop resource with access to information across the entire scope of modern analytical science Presents articles split into three broad areas: analytical techniques, areas of application and and analytes, creating an ideal resource for students, researchers and professionals Provides concise and accessible information that is ideal for non-specialists and readers from undergraduate levels and higher
Download or read book Miniaturization of High Throughput Screening in Microfluidics Based on Plugs and SlipChip written by Liang Li and published by . This book was released on 2010 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation describes the development of microfluidic systems based on plugs and SlipChip, for applications in high throughput screening. The advancement of high throughput screening and assays benefits from miniaturization of fluid handling. Robotic systems automate the process of pipette-dispensing solutions into well plates and run on a much smaller scale, but these systems are expensive and inherit some intrinsic problems from dispensing solutions into open wells. Microfluidics handles small amounts (nanoliters to femtoliters) of fluids in enclosed chambers or channels; consequently, it overcomes the intrinsic problems in robotic systems. Among microfluidics-based systems, valve-based microfluidics, compact-disc (CD)-based microfluidics, and droplet (plug)-based microfluidics are applicable to high throughput screening and assays. Valve-based and CD-based microfluidics performs thousands of nanoliter experiments in parallel, but the devices are complicated to manufacture and require expensive instrumentation to operate. Droplet (plug)-based microfluidics drives fluids by pumps and droplets form spontaneously, therefore it is less complicated. This thesis will discuss the development and improvement of methods using plug-based microfluidics to perform high throughput screening. It will also describe the development of the SlipChip, a new technology that accurately manipulates nanoliter fluids without any instruments.
Download or read book Directed Enzyme Evolution written by Frances H. Arnold and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity clones early in evolution; (3) sufficiently reproducible to allow one to find small improvements; (4) robust, which means that the signal afforded by active clones is not dependent on difficult-to-control environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum “you get what you screen for. ” The protocols in Directed Enzyme Evolution describe a series of detailed p- cedures of proven utility for directed evolution purposes. The volume begins with several selection strategies for enzyme evolution and continues with assay methods that can be used to screen enzyme libraries. Genetic selections offer the advantage that functional proteins can be isolated from very large libraries s- ply by growing a population of cells under selective conditions.
