Download or read book Microfluidic Systems for Investigating and Modulating Single cell Cancer Invasion Dynamics written by Michael Mak and published by . This book was released on 2014 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: My research entails the design and implementation of microfluidic systems to investigate the mechanical dynamics in cancer invasion at the single -cell level. Metastasis, the process of cancer spreading, is the leading cause of cancer related deaths, but the principles and dynamics that drive this process remain largely unknown. The challenge is in part due to the large spatial and temporal scales that the metastatic process spans, as it could involve single-cells transporting to distal sites across meters and over months to years. To address this challenge, my goal is to create microsystems that aim to recapitulate the critical steps in dissemination. Cell scaled microchannels with subnucleus-scaled barriers are incorporated to elucidate the mechanical transition dynamics of invasive cancer cells. Single-cell - single-barrier interactions are induced and invasive behavior is elicited. Different regulators of invasion are explored, including molecular modulators of microtubules and actomyosin as well as mechanical factors such as dimensional, directional, and other engineered spatially asymmetric cues. Results of my work have shown that microtubule stabilization suppresses cell invasion across subnucleus-scaled barriers, physiologically ubiquitous mechanical cues such as dimensionality and directionality modulate migratory cell decision making, myosin IIa activity inhibition can alter invasion patterns, and certain mechanically asymmetric microenvironments can potentially suppress dissemination via the phenomenon iteratio ad nauseam.
Download or read book Investigating Single Cell Growth Dynamics of Mycrobacteria with Microfluidics written by Solmaz Ariana Golchin and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidics for Investigating Single cell Biodynamics written by Scott Warren Cookson and published by . This book was released on 2008 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress in synthetic biology requires the development of novel techniques for investigating long-term dynamics in single cells. Here, we demonstrate the utility of microfluidics for investigating single-cell biodynamics within tightly-controlled environments in the model organisms Saccharomyces cerevisiae and Escherichia coli. First, we develop a microfluidic chemostat for monitoring single-cell gene expression within large populations of S. cerevisiae over many cellular generations. We overcome typical difficulties in tracking individual cells throughout long sequences of time-lapse microscopy images by constraining colony growth to a monolayer. Second, we construct a variant of this device to elucidate a new mechanism of cellular ordering within dense E. coli populations. By comparing colony growth and alignment inside a long and narrow microfluidic channel with continuum models and discrete element simulations, we conclude that the observed dynamic transition from an isotropic disordered phase to a nematic ordered phase is caused by biomechanical interactions arising from cellular growth and division. Finally, we use microfluidics and time-lapse microscopy to characterize an engineered genetic oscillator in E. coli. We find the circuit to exhibit fast and robust oscillatory periods that can be tuned by altering inducer levels, temperature, and the growth medium. Together, these studies illustrate the role of novel measurement techniques in advancing the goals of synthetic biology. Specifically, the ability to generate single-cell expression profiles for a large number of cells is essential to understanding the roles of regulatory motifs within native and synthetic gene networks. Through such an understanding, we aim to develop an engineering-based approach to building gene-regulatory circuits, where design specifications generated from computational modeling drive the construction of regulatory networks with desired properties.
Download or read book Systems Biology of Cancer written by Sam Thiagalingam and published by Cambridge University Press. This book was released on 2015-04-09 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: An overview of the current systems biology-based knowledge and the experimental approaches for deciphering the biological basis of cancer.
Download or read book Essentials of Single Cell Analysis written by Fan-Gang Tseng and published by Springer. This book was released on 2016-01-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Download or read book Integrated Microfluidic Device for Single cell High Throughput Screening in Dynamic Gene Expression Analysis written by Lawrence Kwan Yeung Hui and published by . This book was released on 2008 with total page 60 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past decade, interest in microfluidics has surged as applications have trended towards novel biological assays. Specifically, the ability of microfluidics to parallelize cellular studies through array-based chip designs has attracted researchers interested in investigating cellular function under a wide variety of environmental conditions. The capability of microfluidic devices to control microenvironment conditions and induce dynamic perturbation to cellular systems makes microfluidics (or "lab-on-a-chip") an attractive platform to study gene expression dynamics. In this project, the functionality of microfluidic technology is exploited to design and construct a device for isolation and observation of cells in high throughput. The integration of a concentration gradient with homogenous medium within each chamber was designed specifically to investigate gene regulation in Saccharomyces cerevisiae under various concentrations of chemical inducers. These devices were designed to sustain cells for extended periods of time with high temporal resolution to study dynamic gene expression in single cells. The device builds on previous studies by probing up to eight distinct cell cultures in parallel. The microfluidic platform was then used to study yeast cells at various levels of inducer perturbations. Further experimentation revealed the utility of a parallel gradient by producing an induction curve of the yeast response. Such high-throughput designs will prove essential to yeast systems biology research as it strives to understand the complex regulatory interactions that dictate cell function by probing vast regions of parameter space.
Download or read book Silicon Carbide Technology for Advanced Human Healthcare Applications written by Stephen E. Saddow and published by Elsevier. This book was released on 2022-07-13 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: After over two decades of focused research and development, silicon carbide (SiC) is now ready for use in the healthcare sector and Silicon Carbide Technology for Advanced Human Healthcare Applications provides an up-to-date assessment of SiC devices for long-term human use. It explores a plethora of applications that SiC is uniquely positioned for in human healthcare, beginning with the three primary areas of technology which are closest to human trials and thus adoption in the healthcare industry: neural implants and spinal cord repair, graphene and biosensors, and finally deep tissue cancer therapy using SiC nanotechnology. Biomedical-inspired engineers, scientists, and healthcare professionals will find this book to be very useful in two ways: (I) as a guide to new ways to design and develop advanced medical devices and (II) as a reference for new developments in the field. The book's intent is to stimulate ideas for further technological enhancements and breakthroughs, which will provide alternative solutions for human healthcare applications. - Discusses the utilization of SiC materials for biomedical applications - Provides a logical pathway to understand why SiC is ideal for several critical applications, in particular for long-term implantable devices, and will serve as a guide to new ways to design and develop advanced medical devices - Serves as a reference for new developments in the field and as a technology resource for medical doctors and practitioners looking to identify and implement advanced engineering solutions to everyday medical challenges that currently lack long-term, cost-effective solutions
Download or read book Nanotechnology for Microfluidics written by Xingyu Jiang and published by John Wiley & Sons. This book was released on 2020-09-08 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book focuses on microfluidics with applications in nanotechnology. The first part summarizes the recent advances and achievements in the field of microfluidic technology, with emphasize on the the influence of nanotechnology. The second part introduces various applications of microfluidics in nanotechnology, such as drug delivery, tissue engineering and biomedical diagnosis.
Download or read book Theoretical Microfluidics written by Henrik Bruus and published by Oxford University Press. This book was released on 2007-09-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics is a young and rapidly expanding scientific discipline, which deals with fluids and solutions in miniaturized systems, the so-called lab-on-a-chip systems. It has applications in chemical engineering, pharmaceutics, biotechnology and medicine. As the lab-on-a-chip systems grow in complexity, a proper theoretical understanding becomes increasingly important. The basic idea of the book is to provide a self-contained formulation of the theoretical framework of microfluidics, and at the same time give physical motivation and examples from lab-on-a-chip technology. After three chapters introducing microfluidics, the governing equations for mass, momentum and energy, and some basic flow solutions, the following 14 chapters treat hydraulic resistance/compliance, diffusion/dispersion, time-dependent flow, capillarity, electro- and magneto-hydrodynamics, thermal transport, two-phase flow, complex flow patterns and acousto-fluidics, as well as the new fields of opto- and nano-fluidics. Throughout the book simple models with analytical solutions are presented to provide the student with a thorough physical understanding of order of magnitudes and various selected microfluidic phenomena and devices. The book grew out of a set of well-tested lecture notes. It is with its many pedagogical exercises designed as a textbook for an advanced undergraduate or first-year graduate course. It is also well suited for self-study.
Download or read book Advances in Clinical Chemistry written by Gregory S. Makowski and published by Academic Press. This book was released on 2020-09-20 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Clinical Chemistry, Volume 99, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists. The serial discusses the latest and most up-to-date technologies related to the field of clinical chemistry, with this release including chapters on Extracellular vesicle associated proteins as potential biomarkers, Molecular and non-molecular approaches to etiologic diagnosis of gastroenteritis, Circular RNAs and cancer: Opportunities and challenges, Mass spectrometry-based metabolomics for an in-depth questioning of human health, Application of microfluidic technology in cancer research and therapy, Advances in aptasensor technology, and much more. - Covers the most up-to-date technologies in clinical chemistry and clinical laboratory science - Authored by world renowned clinical laboratory scientists, physicians and research scientists - Provides the international benchmark for novel analytical approaches in the clinical laboratory
Download or read book Analytical Instrumentation Handbook written by Jack Cazes and published by CRC Press. This book was released on 2004-11-30 with total page 1066 pages. Available in PDF, EPUB and Kindle. Book excerpt: Compiled by the editor of Dekker's distinguished Chromatographic Science series, this reader-friendly reference is as a unique and stand-alone guide for anyone requiring clear instruction on the most frequently utilized analytical instrumentation techniques. More than just a catalog of commercially available instruments, the chapters are wri
Download or read book Microfluidic Lab on a Chip for Chemical and Biological Analysis and Discovery written by Paul C.H. Li and published by CRC Press. This book was released on 2005-11-01 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information. Microfluidic
Download or read book Early Detection and Treatment of Head Neck Cancers written by Rami El Assal and published by Springer Nature. This book was released on 2021-05-07 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: Head and neck cancer (HNC) is a heterogeneous group of cancers that, if combined, represent one of the most common cancer types. Patients with HNC suffer significant morbidity and mortality due to the importance of the structures involved. Over two-thirds of these patients are diagnosed at a late stage, leading to a poor prognosis. Therefore, advancements in early detection and treatment of HNC are crucial. Volume I begins with a general overview, including the industry landscape, of HNC detection, diagnosis, and treatment. Next, it covers the applications of innovative technologies such as microfluidics, nanotechnology, and deep learning to early detect as well as study HNC. For example, studying the cellular features at a single-cell level became possible with the advancement of technologies such as mass cytometry or specifically, Cytometry by Time Of Flight Mass Spectrometry (CyTOF), which has revolutionized the way we can study complex human diseases such as HNC. Finally, the last few chapters are dedicated to describing the standard of care of HNC. The first volume of Early Detection and Treatment of Head & Neck Cancers is highly pertinent to the next generation of interdisciplinary clinicians, scientists, residents, and students who are particularly interested in HNC and in the translation of early detection methods, technologies, and research to clinical practice.
Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.
Download or read book Microfluidics for Single Cell Analysis written by Jin-Ming Lin and published by Springer Nature. This book was released on 2019-08-28 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Download or read book Bioelectrics written by Hidenori Akiyama and published by Springer. This book was released on 2016-09-19 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on bioelectrics, a new multidisciplinary field encompassing engineering and biology with applications to the medical, environmental, food, energy, and biotechnological fields. At present, 15 universities and institutes in Japan, the USA and the EU comprise the International Consortium of Bioelectrics, intended to advance this novel and important research field. This book will serve as an introductory resource for young scientists and also as a textbook for use by both undergraduate and graduate students – the world’s first such work solely devoted to bioelectrics.
Download or read book Cutaneous Wound Healing written by Vincent Falanga and published by CRC Press. This book was released on 2001-08-23 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene therapy, bioengineered skin, and other methods in advanced biology are revolutionizing the treatment of wounds. Written by experts in research and clinical practice, Cutaneous Wound Healing examines the current knowledge and emerging treatment methods. This volume explains the normal molecular and cellular functions that occur when a wound heals, as well as dysfunctional events, such as a chronic wound or an ulcer. Such dysfunctions signal an imbalance in the body, explained here along with possible treatments. The book's mini-atlas is an indispensable reference tool. Dermatologists, plastic surgeons, and general practitioners can benefit from this text.
