Download or read book Microfluidic Platforms for Cell Culture and Microenvironment Control written by Yandong Gao and published by . This book was released on 2011 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidic Cell Culture Systems written by Jeffrey T Borenstein and published by Elsevier. This book was released on 2018-09-12 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems. This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances. Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies. Comprehensively covers strategies and techniques ranging from academic first principles to industrial scale-up approaches. Readers will gain insight into cell-material interactions, microfluidic flow, and design principles. Offers three fundamental types of information: 1) design principles, 2) operation techniques, and 3) background information/perspectives. The book is carefully designed to strike a balance between these three areas, so it will be of use to a broad range of readers with different technical interests and educational levels.
Download or read book Microfluidic Organ on a Chip Revolutionary Platforms for Disease Comprehension and Treatment written by Shi-Cong Tao and published by Frontiers Media SA. This book was released on 2022-01-11 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Existing culture systems have a limited ability to reproduce the complicated and dynamic microenvironment of a functioning organ. To solve the issues of conventional culture techniques, multidisciplinary researchers, involving medical doctors, stem cell and developmental biology experts, engineers and physical scientists, have emerged to innovate methods and devices. A microfluidic organ-on-a-chip (μOOC) is a cell culture device, based on microfluidic technology, which contains continuously perfused chambers with cells to simulate organ-level physiology/pathology. The μOOC is not to build a whole living organ, but rather to synthesize minimal functional units that recapitulate organ-/tissue-level functions. The μOOC can be applied to study not only the convention stimulation on cells by molecular/drugs, but also physical forces (fluid shear stress, cyclic strain and mechanical compression), organ-specific cell-cell intercommunication, and organ-organ coupling responses. There is an emerging need for innovative approaches for the production, control, analysis, and utilization of the μOOC, and even the multiple interconnected μOOC (Human-on-a-Chip). Although the μOOC has attracted much attention and is continuous being studied, there are still many difficult problems to be solved. Some of the most mentioned challenges include microenvironmental (biochemical, biophysical, biomechanical, nutrient, etc.) control, modeling tissue–tissue and multiorgan interactions, and reducing variability (automated control, high-throughput manipulation/analysis, integration of biosensing and etc.).
Download or read book Microfluidics in Cell Biology Part C Microfluidics for Cellular and Subcellular Analysis written by and published by Academic Press. This book was released on 2018-11-22 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics
Download or read book Microfluidics for Cellular Applications written by Gerardo Perozziello and published by Elsevier. This book was released on 2023-04-13 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics for Cellular Applications describes microfluidic devices for cell screening from a physical, technological and applications point-of-view, presenting a comparison with the cell microenvironment and conventional instruments used in medicine. Microfluidic technologies, protocols, devices for cell screening and treatment have reached an advanced state but are mainly used in research. Sections break them down into practical applications and conventional medical procedures and offers insights and analysis on how higher resolutions and fast operations can be reached. This is an important resource for those from an engineering and technology background who want to understand more and gain additional insights on cell screening processes. Outlines the major applications of microfluidic devices in medicine and biotechnology Assesses the major challenges of using microfluidic devices in terms of complexity of the control set-up, ease of use, integration capability, automation level, analysis throughput, content and costs Describes the major fabrication techniques for assembling effective microfluidic devices for bioapplications
Download or read book A Microfluidic System for Mouse Embryonic Stem Cell Culture and Microenvironment Control written by Faisal Moledina and published by . This book was released on 2011 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: The embryonic stem cell (ESC) microenvironment contains various localized physical and biochemical cues to direct cell fate. Current approaches for microenvironmental regulation rely on restricting cell behaviour to control endogenous signals such as secreted ligands. This report presents a microfluidic device that can directly manipulate the removal of autoregulatory ligands from culture and control the activation of Signal Transducer and Activator of Transcription-3 (Stat3) in ESCs. Specifically, the response of Stat3 was measured under diffusive and convective mass transfer regimes. A Brownian dynamics algorithm was also developed to simulate ligand transport and predict cellular response under these conditions. Stat3 activation under perfusion culture was found to depend on flow rate and axial distance in the flow direction. Long-term perfusion also allowed for the formation of a sustained gradient of Stat3 activation that led to selective loss of ESC pluripotency. These results demonstrate the utility of microfluidic culture for stem cell bioengineering applications.
Download or read book Integrated Microfluidic Platforms for Quantitative Analysis of Cell Culture and Uptakes written by Tak For Yu and published by . This book was released on 2009 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Medical and Industrial Applications of Microfluidic based Cell Tissue Culture and Organs on a Chip Advances in Organs on a Chip and Organoids Technologies written by Qasem Ramadan and published by Frontiers Media SA. This book was released on 2019-10-16 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in microfluidics have demonstrated enormous potential of microscale cell culture for biology studies and recognized as instrumental in performing rapid and efficient experiments on small-sample volumes. Microfluidic-based cell culture is an area of research that keeps growing and gaining importance as a prominent technology, able to link scientific disciplines with industrial and clinical applications. In particular, organotypic cell culture and its integration in microfluidic devices would enable the realization of “in vivo-like” cell microenvironment within systems that are more amenable to automation and integration. Such remarkable advancement forms the foundation and motivation to transfer research from the laboratory to the field. Although the microfluidics and cell culture technologies have influenced many areas of science, significant research efforts are currently focus on finding methods to transform drug screening and toxicity testing from a system reliant on high-dose animal studies to one based primarily on human-relevant in vitro models. In line with regulatory developments precluding the use of animal testing, as well as fundamental differences in animal versus human, human in vitro methodologies are required to replace the animal-based testes while permitting physiologically relevant model equivalents for superior prediction. Organs-on-a-chip is an ambitious and rapidly growing technology that promise to bridge the gap between in vivo and in vitro studies and open wide possibilities in medical and industrial applications. However, many challenges are still ahead. This eBook present recent state-of-the-art works and critical reviews in organs-on-a-chip technology which highlight the new advances in this growing field with an emphasis on the interface between technological advancements and high impact applications.
Download or read book Versatile Microenvironment controlled Microfluidic Platforms for Studying Cell Behaviors written by Lijun Li and published by . This book was released on 2021 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microengineering Aligned Collagen Substrates in Microfluidic Systems written by Adeel Ahmed and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Cells in vivo are surrounded by a fibrous matrix of proteins and macromolecules called the extracellular matrix (ECM), of which type I collagen is the major constituent. During tissue development or cell-matrix interactions, collagen fibers organize into aligned domains with defined degrees of alignment and directionality. Aligned fibers guide stem cell differentiation and influence cell-cell communication and cell motility. In the tumor microenvironment, aligned fibers guide tumor cell invasion and have been linked to poor patient outcomes. Since fiber alignment instructs cell behavior in vivo, there is a need for in vitro models to replicate fiber alignment and thus provide a relevant microenvironment for cells. Microfluidic systems have been established as advanced cell culture platforms to provide precise control over soluble factor concentration, cell patterning, and fluid flow. However, controlling the fiber alignment of a 3D material within them has remained a challenge. This work addresses existing technological challenges to integrate 3D collagen matrices with aligned fibers into microfluidic platforms. To do so, this work i) Demonstrates for the first time that extensional flows can align 3D collagen matrices (250 [microns] thick) in a microchannel, ii) Develops modular microfluidic platforms with capabilities to directly access and perfuse 3D collagen, and iii) Develops biofabrication capabilities to create interfaces between different ECM materials in 3D, and create tissue barriers using ultrathin nanomembranes. It is anticipated that the novel ECM microengineering capabilities and approach to integrating the engineered matrices into microfluidic devices will provide a path to develop tissue-specific in vitro models with engineered matrices."--Abstract.
Download or read book Open Microfuidic Platforms for Tissue Culture written by Theodorus Evan De Groot and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The tissue microenvironment is a complex and dynamic system. When microenvironments become dysregulated it can lead to a number of diseases including cancer. Understanding the interactions in the microenvironment is key to understanding disease progression and could lead to the development of new and effective therapies. The complexity of the microenvironment makes the development of in vitro models challenging. A model needs to have the appropriate context to make it a good biological proxy for the system it's designed to investigate, but also needs to simple enough so that researchers can manipulate it to get good data from the model. Microfluidic techniques and technologies are amenable for complex tissue engineering tools to achieve high biological relevance and allow for precise control and manipulation over many signals experienced in the model making them well suited for developing in vitro models. In spite of their benefits, microfluidics has not yet achieved widespread adoption in the biology community. To achieve higher penetration of microfluidics-based in vitro models on the biology bench, engineers need to design systems with not only the biology in mind, but also the biologist. This document describes how choice of material, fabrication method, and how a user interfaces with a microfluidic platform can not only lead a more biology friendly in vitro model, but can enable models that would be difficult of impossible to achieve without these design considerations.
Download or read book Microfluidics in Cell Biology Part A Microfluidics for Multicellular Systems written by and published by Academic Press. This book was released on 2018-07-20 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. Contains contributions from experts in the field from across the globe Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics
Download or read book Microfluidic Platforms for Studying Cell Behavior in Complex Microenvironments written by and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Microfluidic Cell Culture Platforms for Investigating Cellular Phenomena written by Bryson McKinley Brewer and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Integrated Nanoplasmonic Optical Microfluidics for Label free Bioassays written by Yir-shyuan Wu and published by . This book was released on 2009 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current cellular assays are limited to multi-well based cell culture samples, and the existing cellular protein detection methods are restricted to labeling the targeting molecules with fluorescent dyes or other reporters. Microfluidic cell culture technology can provide precise and physiologically relevant microenvironment control to improve the quality of cell based assays. Nanoplasmonic optical probes enable the label-free detection of cellular protein with high temporal and spatial resolution. The goal of this dissertation is to develop and integrate real-time label-free nanoplasmonic detection with high-throughput microfluidic cell culture platforms. In this dissertation, several novel nanoplasmonic geometries, such as crescent-shaped nanoholes and nanocorals are developed to provide sensitive, robust and low-cost solutions for biomolecular detection. The sharp tips and large curvature features on the nanostructures are designed to maximize the sensitivity of both localized surface plasmon resonance (LSPR) peak shift and surface enhanced Raman spectroscopy (SERS) sensing applications. Two robust cell culture platforms - long-term single cell culture arrays and self-assembled tumor spheroid arrays - that facilitate monitoring arrayed cells with precise micro-environment control are also demonstrated. The integration of nanoplasmonic biosensors and microfluidic cell culture platforms are demonstrated on both chip-size and at the whole 4" wafer scale. Such integrated systems provide high resolution dynamic information on large array of precisely controlled cell populations. We expect this new tool will have significant impact on both fundamental cellular research and new drug evaluation.
Download or read book A Microfluidics Based Cell Culture Device with Controlled Temperature Gradient written by and published by . This book was released on 2015 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidics in Cell Biology Part B Microfluidics in Single Cells written by and published by Academic Press. This book was released on 2018-08-27 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics
