Download or read book Microfluidic Devices for Forensic DNA Analysis written by Jayna J. Shah and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The development of integrated, miniaturized, and portable DNA analysis systems is crucial to alleviate massive backlog of unanalyzed samples and to address ever increasing demand for these assays. This thesis work contributes towards the development of a fully integrated microdevice capable of 'sample in -- answer out' for forensic DNA analysis. Specifically, this work describes the development of rapid and robust fabrication protocol for solvent-actuated bonding of polymeric thermoplastic substrates at room temperature, the development of microchannel wall coating strategies to eliminate analyte-wall interactions for high resolution separation of single-stranded DNA, and the characterization of a thin-film planar microwave transmission line for microfluidic heating applications. The solvent-actuated bonding protocol was based on the difference in capillary forces between the microchannel and the interstitial space between the surfaces of the two substrates to be bonded. This force differential wicked the bonding solvent into the gap between the substrates causing them to bond. The technique was implemented by placing the two substrates under moderate pressure, applying a moderate pneumatic vacuum to the fluidic channel, and introducing tens of microliter of bonding solvent through one end of the fluidic channel. The effect of bonding solvent on the dimensions of the microchannel was analyzed, and the mechanical robustness of the bonded devices was also characterized. Electrophoretic separation of single-stranded DNA (ssDNA) was successfully performed to demonstrate the functionality of these devices. To enhance ssDNA separation performance, schemes to modify poly(methyl methacrylate) (PMMA) -- the primary substrate used in this work -- were explored. This two step process consisted of altering surface hydrophilicity via surface activation using either nitric acid or UV/ozone followed by coating the surfaces with adsorptive polymers. Contact-angle measurements of the pristine and modified PMMA substrates were performed to quantify the change in wettability of the surface. Twofold increase in the separation efficiency was achieved by implementing these surface passivation strategies. Finally, the use of a thin-film planar microwave transmission line as a microwave power source was investigated for on-chip microwave heating of fluids. The microwave characterization data was used to develop a first-order analytic model of the microwave power absorption. The model was used to understand microwave power flow through the device and to calculate the fraction of the incident power absorbed in the fluid. Additionally, a fit of the predicted temperature obtained using this model to the measured temperature was performed to evaluate efficiency of this heating method"--Abstract
Download or read book Microfluidic Techniques written by Shelley D. Minteer and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Polymeric Microfluidic Devices for DNA Analysis written by Yi Sun and published by . This book was released on 2010 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Sample Extraction and Processing in Microfluidic DNA Analysis Devices written by Rongsheng Lin and published by . This book was released on 2005 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidic Methods for Molecular Biology written by Chang Lu and published by Springer. This book was released on 2016-05-14 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics
Download or read book Forensic DNA Analysis written by Jaiprakash G. Shewale and published by CRC Press. This book was released on 2013-08-19 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of forensic DNA analysis has grown immensely in the past two decades and genotyping of biological samples is now routinely performed in human identification (HID) laboratories. Application areas include paternity testing, forensic casework, family lineage studies, identification of human remains, and DNA databasing. Forensic DNA Analysis:
Download or read book DNA Purification in Microfluidic Systems for Clinical and Forensic Application written by Joan Marie Bienvenue and published by . This book was released on 2007 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Integrated Modular Microfluidic System for Forensic Alu Dna Typing written by Samuel Njoroge and published by LAP Lambert Academic Publishing. This book was released on 2012 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: To produce a microsystem with favorable performance characteristics for genetic-based analyses, several key operational elements must be strategically chosen, including device substrate material, temperature control, fluidic control and reaction product readout. The advantages afforded by fully integrated microfluidic systems to enable challenging applications, such as single-copy DNA sequencing, single-cell gene expression analysis, pathogen detection, and forensic DNA analysis in formats that provide high throughput and point-of-analysis capabilities must be addressed by the researcher. This book focus on discussion on microfluidic systems that are composed of two or more microdevices directed toward DNA analyses. The discussions primarily focus on the integration of various processing steps with u-capillary electrophoresis (uCE) with discoveries that have led to the development of fully autonomous and functional integrated systems for genome processing that can supply "sample in / answer out" capabilities."
Download or read book Microfluidic Devices for Biomedical Applications written by Xiujun (James) Li and published by Elsevier. This book was released on 2013-10-31 with total page 689 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications.The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis.Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. - Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores in detail a wide range of medical applications - Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies - Considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering
Download or read book Advanced Topics in Forensic DNA Typing Methodology written by John M. Butler and published by Academic Press. This book was released on 2011-07-21 with total page 700 pages. Available in PDF, EPUB and Kindle. Book excerpt: John M. Butler.
Download or read book DNA Purification on Microfluidic Devices with a Focus on Large Volume Forensic Biological Samples written by Carmen Reneé Reedy and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidic Systems for Cancer Diagnosis written by Jose L. Garcia-Cordero and published by Springer Nature. This book was released on 2023-06-10 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores recent developments in microfluidics technologies for cancer diagnosis and monitoring. The book is divided into two sections that delve into techniques for liquid biopsy for cancer diagnosis and platforms for precision oncology or personalized medicine in order to create effective patient avatars for testing anti-cancer drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microfluidic Systems for Cancer Diagnosis serves as an ideal guide that will be helpful to either replicate the construction of microfluidic devices specifically developed for cancer diagnosis or to catalyze development of new and better cancer diagnostic devices.
Download or read book Multidisciplinary Microfluidic and Nanofluidic Lab on a Chip written by Xiujun James Li and published by Elsevier. This book was released on 2021-09-23 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring. Provides calculations, solved problems, data tables and design rules to help understand microfluidic basic theory and principles Gives an applied understanding of the principles behind the design, manufacture, and testing of microfluidic systems Emphasizes on quickly developing and emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology
Download or read book Modern Forensic Tools and Devices written by Deepak Rawtani and published by John Wiley & Sons. This book was released on 2023-05-26 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: MODERN FORENSIC TOOLS AND DEVICES The book offers a comprehensive overview of the latest technologies and techniques used in forensic investigations and highlights the potential impact of these advancements on the field. Technology has played a pivotal role in advancing forensic science over the years, particularly in modern-day criminal investigations. In recent years, significant advancements in forensic tools and devices have enabled investigators to gather and analyze evidence more efficiently than ever. Modern Forensic Tools and Devices: Trends in Criminal Investigation is a comprehensive guide to the latest technologies and techniques used in forensic science. This book covers a wide range of topics, from computer forensics and personal digital assistants to emerging analytical techniques for forensic samples. A section of the book provides detailed explanations of each technology and its applications in forensic investigations, along with case studies and real-life examples to illustrate their effectiveness. One critical aspect of this book is its focus on emerging trends in forensic science. The book covers new technologies such as cloud and social media forensics, vehicle forensics, facial recognition and reconstruction, automated fingerprint identification systems, and sensor-based devices for trace evidence, to name a few. Its thoroughly detailed chapters expound upon spectroscopic analytical techniques in forensic science, DNA sequencing, rapid DNA tests, bio-mimetic devices for evidence detection, forensic photography, scanners, microscopes, and recent advancements in forensic tools. The book also provides insights into forensic sampling and sample preparation techniques, which are crucial for ensuring the reliability of forensic evidence. Furthermore, the book explains the importance of proper sampling and the role it plays in the accuracy of forensic analysis. Audience The book is an essential resource for forensic scientists, law enforcement officials, and anyone interested in the advancements in forensic science such as engineers, materials scientists, and device makers.
Download or read book Cell Analysis on Microfluidics written by Jin-Ming Lin and published by Springer. This book was released on 2017-10-25 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.
Download or read book Secure and Trustworthy Cyberphysical Microfluidic Biochips written by Jack Tang and published by Springer. This book was released on 2019-05-28 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes novel hardware security and microfluidic biochip design methodologies to protect against tampering attacks in cyberphysical microfluidic biochips (CPMBs). It also provides a general overview of this nascent area of research, which will prove to be a vital resource for practitioners in the field.This book shows how hardware-based countermeasures and design innovations can be a simple and effective last line of defense, demonstrating that it is no longer justifiable to ignore security and trust in the design phase of biochips.
Download or read book Optimization of Trustworthy Biomolecular Quantitative Analysis Using Cyber Physical Microfluidic Platforms written by Mohamed Ibrahim and published by CRC Press. This book was released on 2020-05-31 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: A microfluidic biochip is an engineered fluidic device that controls the flow of analytes, thereby enabling a variety of useful applications. According to recent studies, the fields that are best set to benefit from the microfluidics technology, also known as lab-on-chip technology, include forensic identification, clinical chemistry, point-of-care (PoC) diagnostics, and drug discovery. The growth in such fields has significantly amplified the impact of microfluidics technology, whose market value is forecast to grow from $4 billion in 2017 to $13.2 billion by 2023. The rapid evolution of lab-on-chip technologies opens up opportunities for new biological or chemical science areas that can be directly facilitated by sensor-based microfluidics control. For example, the digital microfluidics-based ePlex system from GenMarkDx enables automated disease diagnosis and can bring syndromic testing near patients everywhere. However, as the applications of molecular biology grow, the adoption of microfluidics in many applications has not grown at the same pace, despite the concerted effort of microfluidic systems engineers. Recent studies suggest that state-of-the-art design techniques for microfluidics have two major drawbacks that need to be addressed appropriately: (1) current lab-on-chip systems were only optimized as auxiliary components and are only suitable for sample-limited analyses; therefore, their capabilities may not cope with the requirements of contemporary molecular biology applications; (2) the integrity of these automated lab-on-chip systems and their biochemical operations are still an open question since no protection schemes were developed against adversarial contamination or result-manipulation attacks. Optimization of Trustworthy Biomolecular Quantitative Analysis Using Cyber-Physical Microfluidic Platforms provides solutions to these challenges by introducing a new design flow based on the realistic modeling of contemporary molecular biology protocols. It also presents a microfluidic security flow that provides a high-level of confidence in the integrity of such protocols. In summary, this book creates a new research field as it bridges the technical skills gap between microfluidic systems and molecular biology protocols but it is viewed from the perspective of an electronic/systems engineer.
