Download or read book Microfluidic and Electronic Detection of Protein Biomarkers written by Dan Wu (Ph.D.) and published by . This book was released on 2020 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are essential components of our bodies and play critical roles in biological processes. Quantifying protein levels can help characterize these biological processes and detect malfunctions. Thus, protein testing is widely utilized in clinical diagnostics. The current workflow for protein testing involves the processing of patient samples that are collected at healthcare facilities. These samples are sent to centralized laboratories and analyzed using sophisticated instruments. As a result, it can take days to deliver results. However, to manage acute conditions such as sepsis, rapid results are desired to ensure timely diagnosis and treatment. In contrast, point-of-care testing has shown great promise for rapid results through the use of miniaturized and inexpensive devices in non-laboratory settings. While there have been many advances in this field, only a small number of proteins (e.g., cardiac biomarkers) are covered by point-of-care devices, due to the profound challenge of maintaining sensitivity while miniaturizing testing system and reducing assay time. In this thesis, this technical challenge was tackled to develop a point-of-care system for the measurement of interleukin-6, which can be used as a biomarker for sepsis or cytokine release syndrome. Inspired by the success of commercial glucose meters, an all-electrical system was developed, due to easy miniaturization and low cost of electronics. First, surface chemistries were developed to coat antibodies onto electrodes and successful surface modification was validated via various techniques. Non-Faradaic impedimetric, Faradaic impedimetric and chemiresistive label-free electrical biosensors were developed and examined. However, it was found that these biosensing platforms are susceptible to drifts due to the non-specific nature of the signal transduction. Then, building on the enzyme-linked immunosorbent assay (ELISA, the gold standard), a bead-based electronic ELISA was developed, where beads expedite the testing and electrical readout replaces the colorimetric readout in the gold standard ELISA. An integrated mathematical model was developed to comprehensively understand the assay and inform optimization. While providing comparable limit of detection with the gold standard ELISA (

Download or read book Portable Microfluidic Fluid Handling System for the Detection of Protein Biomarkers in Whole Human Blood written by Michael Franzblau and published by . This book was released on 2014 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Electrochemical Detection of Multiple Cancer Protein Biomarkers Using Microfluidic Device written by Bhaskara V. Chikkaveeraiah and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microfluidic Device for Rapid Simultaneous Detection of Dna Rna and Protein Biomarkers in Single Cells written by and published by . This book was released on 2015 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microfluidic Biosensors written by Wing Cheung Mak and published by Elsevier. This book was released on 2022-11-05 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidic Biosensors provides a comprehensive overview covering the most recent emerging technologies on the design, fabrication, and integration of microfluidics with transducers. These form various integrated microfluidic biosensors with device configurations ranging from 2D to 4D levels. Coverage also includes advanced printed microfluidic biosensors, flexible microfluidics for wearable biosensors, autonomous lab-on-a-chip biosensors, CMOS-base microanalysis systems, and microfluidic devices for mobile phone biosensing. The editors and contributors of this book represent both academia and industry, come from a varied range of backgrounds, and offer a global perspective. This book discusses the design and principle of microfluidic systems and uses them for biosensing applications. The microfluidic fabrication technologies covered in this book provide an up-to-date view, allowing the community to think of new ways to overcome challenges faced in this field. The focus is on existing and emerging technologies not currently being analyzed extensively elsewhere, providing a unique perspective and much-needed content. The editors have crafted this book to be accessible to all levels of academics from graduate students, researchers, and professors working in the fields of biosensors, microfluidics design, material science, analytical chemistry, biomedical devices, and biomedical engineering. It can also be useful for industry professionals working for microfluidic device manufacturers, or in the industry of biosensors and biomedical devices. Presents an in-depth overview of microfluidic biosensors and associated emerging technologies such as printed microfluidics and novel transducers Addresses a range of microfluidic biosensors with device configurations ranging from 2D to 4D levels Includes the commercialization aspects of microfluidic biosensors that provide insights for scientists and engineers in research and development

Download or read book Semi automated Ultrasensitive Electrochemical Microfluidic Immunoarray for On line Capture and Detection of Cancer Protein Biomarkers written by Brunah Akinyi Otieno and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Early cancer diagnoses promise to improve treatment outcomes and patient survival rates. However, current cancer diagnostics often rely on finding a tumor, making early detection difficult and possibly compromising therapy outcomes. Screening for cancer without tumor detection can be based on assays of body fluids such as serum or plasma for cancer biomarker proteins to provide an instantaneous record of a patient’s disease status. Measurement of these biomarker proteins should be accurate, sensitive, cheap and preferably at point-of-care (POC) for translation to the clinic. In addition, due to low predictive power of some biomarker proteins and their over-expression by multiple cancer types, it is essential to measure panels of biomarker proteins as opposed to a single biomarker for reliable diagnosis of cancer. Simultaneous measurement of the levels of a panel of biomarker proteins can lead to more accurate diagnoses and offer clarity about disease characteristics and progression that may help guide treatment strategies. The long-term goal of this thesis research is to provide multiple biomarker-based diagnostics to enable accurate early detection of cancer and therapy monitoring. A simple, low-cost, 8-electrode semi-automated modular microfluidic system for on-line capture and electrochemical detection of panels of cancer protein biomarkers was designed and validated. Proteins that were validated include oral cancer biomarkers (IL-6 IL-8) and oral mucositis biomarkers (IL-6, TNF-α, IL-1Î2 and CRP). This thesis also describes our first electrochemical assay for parathyroid hormone related peptide (PTHrP); a paraneoplastic peptide responsible for hypercalcemia in cancer patients and also implicated in cancer metastasis. Also described in the dissertation, is a novel 16-electrode electrochemical set up for simultaneous detection of upto 8-prostate cancer biomarkers, many of which are thought to be specific for aggressive prostate cancer. Measurement of these small panels of selective biomarkers holds remarkable promise for future cancer diagnostics and personalized cancer therapies and most importantly in identifying aggressive vs indolent forms of prostate cancer.

Download or read book Immunosensing for Detection of Protein Biomarkers written by Huangxian Ju and published by Elsevier. This book was released on 2017-05-18 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunosensing for Detection of Protein Biomarkers not only introduces the principles, methods, and classification of immunoassay, but also presents the latest achievements in areas such as electrochemical immunosensors, nanoprobe-based immunoassay, chemiluminescence immunoassay, electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging for immunoassay, signal amplification for immunoassay, and so on. In recent years, immunosensing and immunoassay methods have attracted considerable interest due to their applications in different fields, particularly clinical diagnosis. Although a large number of academic papers in immunosensing and immunoassay have been published in different journals recently, it is still a difficult and time-consuming task for researchers, especially those new to the area, to understand the principles, methods, and research progress of immunosensing. Based on the research experience of the authors and their research groups, this book offers readers with new research ideas to develop immunosensing methodology. As a monograph, it offers deeper and more complete coverage than review papers, which only report certain aspects of progress. Grounded in the research experience of Professor Ju’s research group, the book focuses on immunosensing for detection of protein biomarkers, summarizing understanding, research, and practice on immunosensing methodology in detection of protein biomarkers. Presents the latest research and thinking on immunosensing for detection of protein biomarkers Offers current techniques, and looks to the development of new methodologies Offers the latest developments in various aspects of immunosensing, including electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging immunoassay, and signal amplification immunoassay Offers readers new ideas to research and develop immunosensing methodologies for the future

Download or read book Electrochemical Based Detection of Cancer Biomarker Proteins Using Multi labeled Magnetic Particles in Microfluidic Systems written by and published by . This book was released on 2015 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of a Microfluidic Immunoassay Platform for the Rapid Quantification of Low picomolar Concentrations of Protein Biomarkers written by Marc Herrmann and published by . This book was released on 2008 with total page 149 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microfluidics and Biosensors in Cancer Research written by David Caballero and published by Springer Nature. This book was released on 2022-06-27 with total page 599 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers a comprehensive overview of the development and application of microfluidics and biosensors in cancer research, in particular, their applications in cancer modeling and theranostics. Over the last decades, considerable effort has been made to develop new technologies to improve the diagnosis and treatment of cancer. Microfluidics has proven to be a powerful tool for manipulating biological fluids with high precision and efficiency and has already been adopted by the pharmaceutical and biotechnology industries. With recent technological advances, particularly biosensors, microfluidic devices have increased their usefulness and importance in oncology and cancer research. The aim of this book is to bring together in a single volume all the knowledge and expertise required for the development and application of microfluidic systems and biosensors in cancer modeling and theranostics. It begins with a detailed introduction to the fundamental aspects of tumor biology, cancer biomarkers, biosensors and microfluidics. With this knowledge in mind, the following sections highlight important advances in developing and applying biosensors and microfluidic devices in cancer research at universities and in the industry. Strategies for identifying and evaluating potent disease biomarkers and developing biosensors and microfluidic devices for their detection are discussed in detail. Finally, the transfer of these technologies into the clinical environment for the diagnosis and treatment of cancer patients will be highlighted. By combining the recent advances made in the development and application of microfluidics and biosensors in cancer research in academia and clinics, this book will be useful literature for readers from a variety of backgrounds. It offers new visions of how this technology can influence daily life in hospitals and companies, improving research methodologies and the prognosis of cancer patients.

Download or read book Foundational Studies for Array based Electrophoretic Exclusion of Proteins written by Fanyi Zhu and published by . This book was released on 2019 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Disease prevention and personalized treatment will be impacted by the continued integration of protein biomarkers into medical practice. While there are already numerous biomarkers used clinically, the detection of protein biomarkers among complex matrices remains a challenging problem. One very important strategy for improvements in clinical application of biomarkers is separation/preconcentration, impacting the reliability, efficiency and early detection. Electrophoretic exclusion can be used to separate, purify, and concentrate biomarkers. This counterflow gradient technique exploits hydrodynamic flow and electrophoretic forces to exclude, enrich, and separate analytes. The development of this technique has evolved onto an array-based microfluidic platform which offers a greater range of geometries/configurations for optimization and expanded capabilities and applications. Toward this end of expanded capabilities, fundamental studies of subtle changes to the entrance flow and electric field configurations are investigated. Three closely related microfluidic interfaces are modeled, fabricated and tested. A charged fluorescent dye is used as a sensitive and accurate probe to test the concentration variation at these interfaces. Models and experiments focus on visualizing the concentration profile in areas of high temporal dynamics, and show strong qualitative agreement, which suggests the theoretical assessment capabilities can be used to faithfully design novel and more efficient interfaces. Microfluidic electrophoretic separation technique can be combined with electron microscopy as a protein concentration/purification step aiding in sample preparation. The integrated system with grids embedded into the microdevice reduces the amount of time required for sample preparation to less than five minutes. Spatially separated and preconcentrated proteins are transferred directly from an upstream reservoir onto grids. Dilute concentration as low as 0.005 mg/mL can be manipulated to achieve meaningful results. Selective concentration of one protein from a mixture of two proteins is also demonstrated. Electrophoretic exclusion is also used for biomarker applications. Experiments using a single biomarker are conducted to assess the ability of the microdevice for enrichment in central reservoirs. A mixture of two protein biomarkers are performed to evaluate the proficiency of the device for separations capability. Moreover, a battery is able to power the microdevice, which facilitates the future application as a portable device.

Download or read book The Detection of Biomarkers written by Sibel A. Ozkan and published by Academic Press. This book was released on 2021-12-05 with total page 615 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reliable, precise and accurate detection and analysis of biomarkers remains a significant challenge for clinical researchers. Methods for the detection of biomarkers are rather complex, requiring pre-treatment steps before analysis can take place. Moreover, comparing various biomarker assays and tracing research progress in this area systematically is a challenge for researchers. The Detection of Biomarkers presents developments in biomarker detection, including methods tools and strategies, biosensor design, materials, and applications. The book presents methods, materials and procedures that are simple, precise, sensitive, selective, fast and economical, and therefore highly practical for use in clinical research scenarios. This volume situates biomarker detection in its research context and sets out future prospects for the area. Its 20 chapters offer a comprehensive coverage of biomarkers, including progress on nanotechnology, biosensor types, synthesis, immobilization, and applications in various fields. The book also demonstrates, for students, how to synthesize and immobilize biosensors for biomarker assay. It offers researchers real alternative and innovative ways to think about the field of biomarker detection, increasing the reliability, precision and accuracy of biomarker detection. Locates biomarker detection in its research context, setting out present and future prospects Allows clinical researchers to compare various biomarker assays systematically Presents new methods, materials and procedures that are simple, precise, sensitive, selective, fast and economical Gives innovative biomarker assays that are viable alternatives to current complex methods Helps clinical researchers who need reliable, precise and accurate biomarker detection methods

Download or read book Microfluidic Devices with Integrated Sample Preparation for Improved Analysis of Protein Biomarkers written by Pamela Nsang Nge and published by . This book was released on 2012 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt: To fabricate polymeric microdevices, I prepared templates from silicon wafers by the process of photolithography. The design on the template was transferred to a polymer piece by hot embossing, and a complete device was formed by bonding the imprinted piece with a cover plate. I prepared affinity columns in these devices and used them for protein extraction. The affinity monolith was prepared from reactive monomers to faciliate immobilization of antibodies. Extraction and concentration of biomarkers on this column showed specificity to the target molecule. This shows that biomarkers could be extracted, purified and concentrated with the use of microfluidic affinity columns.

Download or read book Microfluidics Diagnostics written by Valérie Taly and published by Springer Nature. This book was released on with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Principles of Measurement and Transduction of Biomedical Variables written by Vera Button and published by Academic Press. This book was released on 2015-04-07 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Measurement and Transduction of Biomedical Variables is a comprehensive text on biomedical transducers covering the principles of functioning, application examples and new technology solutions. It presents technical and theoretical principles to measure biomedical variables, such as arterial blood pressure, blood flow, temperature and CO2 concentration in exhaled air and their transduction to an electrical variable, such as voltage, so they can be more easily quantified, processed and visualized as numerical values and graphics. The book includes the functioning principle, block diagram, modelling equations and basic application of different transducers, and is an ideal resource for teaching measurement and transduction of biomedical variables in undergraduate and postgraduate biomedical engineering programs. Will help you to understand the design and functioning of biomedical transducers through practical examples and applied information Covers MEMS and laser sensors Reviews the range of devices and techniques available plus the advantages and shortcomings for each transducer type

Download or read book Advancing Microfluidic based Protein Biosensor Technology for Use in Clinical Diagnostics written by Seokheun Choi and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Demand for biosensor research applications is growing steadily. According to a new report by Frost & Sullivan, the biosensor market is expected to reach $14.42 billion by 2016. Clinical diagnostic applications continue to be the largest market for biosensors, and this demand is likely to continue through 2016 and beyond. Biosensor technology for use in clinical diagnostics, however, requires translational research that moves bench science and theoretical knowledge toward marketable products. Despite the high volume of academic research to date, only a handful of biomedical devices have become viable commercial applications. Academic research must increase its focus on practical uses for biosensors. This dissertation is an example of this increased focus, and discusses work to advance microfluidic-based protein biosensor technologies for practical use in clinical diagnostics. Four areas of work are discussed: The first involved work to develop reusable/reconfigurable biosensors that are useful in applications like biochemical science and analytical chemistry that require detailed sensor calibration. This work resulted in a prototype sensor and an in-situ electrochemical surface regeneration technique that can be used to produce microfluidic-based reusable biosensors. The second area of work looked at non-specific adsorption (NSA) of biomolecules, which is a persistent challenge in conventional microfluidic biosensors. The results of this work produced design methods that reduce the NSA. The third area of work involved a novel microfluidic sensing platform that was designed to detect target biomarkers using competitive protein adsorption. This technique uses physical adsorption of proteins to a surface rather than complex and time-consuming immobilization procedures. This method enabled us to selectively detect a thyroid cancer biomarker, thyroglobulin, in a controlled-proteins cocktail and a cardiovascular biomarker, fibrinogen, in undiluted human serum. The fourth area of work involved expanding the technique to produce a unique protein identification method; Pattern-recognition. A sample mixture of proteins generates a distinctive composite pattern upon interaction with a sensing platform consisting of multiple surfaces whereby each surface consists of a distinct type of protein pre-adsorbed on the surface. The utility of the "pattern-recognition" sensing mechanism was then verified via recognition of a particular biomarker, C-reactive protein, in the cocktail sample mixture.

Download or read book Diagnostic Devices with Microfluidics written by Francesco Piraino and published by CRC Press. This book was released on 2017-06-26 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a current view of the research and commercial landscape of diagnostics devices, particularly those that utilize microscale technologies, intended for both patient and laboratory use. Common diagnostic devices that are based on microfluidic principles include glucose sensors for diabetic patients and over-the-counter pregnancy tests. Other diagnostic devices are being developed to quickly test a patient for bacterial and viral infections, and other diseases. The chapters, written by experts from around the world, discuss how to fabricate, apply, and market microfluidic diagnostic chips – for lab and at-home use. Most importantly, the book also contains a discussion of topics relevant to the private sector, including patient-focused, market-oriented development of diagnostics devices. Chapter 9 of this book is freely available as a downloadable Open Access PDF under a CC-BY 3.0 license. https://s3-us-west-2.amazonaws.com/tandfbis/rt-files/docs/Open+Access+Chapters/9781498772938_oachapter9.pdf