Download or read book Methods of Adipose Tissue Biology Part A written by and published by Academic Press. This book was released on 2014-01-27 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale. The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels
Download or read book Methods of Adipose Tissue Biology Part B written by and published by Academic Press. This book was released on 2014-02-11 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale. The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels
Download or read book Methods of Adipose Tissue Biology written by Ormond A. Macdougald and published by Academic Press. This book was released on 2014 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale. 1) The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications. 2) Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues. 3) The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels.
Download or read book Methods of Adipose Tissue Biology Part A written by and published by Academic Press. This book was released on 2014-02-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.
Download or read book Adipose Tissue Biology written by Michael E. Symonds and published by Springer Science & Business Media. This book was released on 2011-11-20 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to provide a comprehensive insight into current perspectives and challenges in adipose tissue biology. In Adipose Tissue Biology, scientists and clinicians discuss adipocyte precursors, differentiation and growth, brown and white adipose tissue, gender, inflammation, dietary and genetic determinants of fat mass, together with evolutionary and developmental aspects of adiposity.
Download or read book Brown Adipose Tissue written by David A. Guertin and published by Springer Nature. This book was released on 2022-02-15 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes. The content reflects the advancing technologies in genetics, imaging, and 'omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, which remain the core tenets of BAT biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brown Adipose Tissue: Methods and Protocols provides perspectives and detailed protocols for the benefit of both new BAT researchers looking for guidance as well as seasoned researchers who would like to expand their toolkits. Chapter 12 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Adipose Derived Stem Cells written by Jeffrey M. Gimble and published by Springer Nature. This book was released on with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Adipose Tissue Protocols written by Gérard Ailhaud and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduction and good health. Adipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. Unfortunately, excess WAT leads to obesity, which is the most common health problem in industrialized countries. Therefore, from both a scientific and a technical point of view, the time has come to create a survey of adipose tissues and their neglected adipocytes. In Adipose Tissue Protocols, I have attempted to gather together chapters from all areas of adipose tissue research—from in vivo to in vitro studies—and to provide methods covering a wide variety of techniques, including the choice of adipose tissue depot and of morphological techniques for the study of BAT and WAT; the isolation, subcellular fractionation, and transfection of adipocytes where the low density of these cells must be taken into account; assays of nutrient and ion fluxes and the metabolic aspects of nutrient uptake; assays of lipid-related enzymes; biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT of various species, including human tissue; measurements of adipose secretory products; and assessment of WAT metabolism in vivo.
Download or read book Adipose Tissue Biology written by Michael E. Symonds and published by Springer. This book was released on 2017-04-03 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen an exponential increase in our knowledge and understanding of adipose tissue biology. This has coincided with the continued rise in obesity across all generations. Clearly despite substantial advances in research into adipose tissue this still has had limited impact on the on-going obesity epidemic across a majority of countries in the world. This book brings together many leading experts in the field to provide an up to date and comprehensive review of the key aspects of adipose tissue. It therefore includes chapters on evolution, development and inflammation together with a detailed review of brown and beige adipose tissue biology and their potential significance in preventing or combating obesity. These chapters are complemented by those on genetics and gender influences, together with nutrition through the life cycle. Ultimately the book provides an overview of the complexities of adipose tissue biology and the continuing challenge to combat obesity in the 21st century.
Download or read book Adipose Tissue Protocols written by Kaiping Yang and published by Humana Press. This book was released on 2010-10-28 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: The radical expansion and revision of techniques and experimental approaches employed in the study of adipose tissue are reflected in this revised book. Several updated chapters are combined with many new contributions from internationally recognized experts. Conventional and state-of-the-art methodologies are covered, as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. This revised all-in-one manual is essential reading for newcomers to this burgeoning area as well as a valuable volume for more experienced researchers.
Download or read book Scientific Principles of Adipose Stem Cells written by Lauren Kokai and published by Academic Press. This book was released on 2021-08-19 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Principles of Adipose Stem Cells provides readers with in-depth and expert knowledge on adipose stem cells, their developmental biologic origins, foundational research on ASC signaling mechanisms and immunomodulatory properties, and clinical insights into applications in regenerative medicine. Topics covered include basic adipose stem cell developmental biology and mechanisms of regulating self-renewal and activation in the stem cell niche, important methods for isolation and characterizing ASCs, and data on the impact on human demographics (age, sex, BMI) on ASC phenotype. A section devoted to ASC biology, ASCs for stem cell therapy and regenerative medicine, and ASCs in tissue engineering applications are also included. The book is written for scientists and clinicians who are broadly familiar with stem cells and basic cell biology principles and those seeking advanced information on adipose stem cells. Coverage of basic adipose stem cell developmental biology (maturation process during embryogenesis) and mechanisms of regulating self-renewal and activation in the stem cell niche Includes important methods for isolation and characterizing ASCs, as well as known data any impact of human demographics (age, sex, BMI) on ASC phenotype An entire section dedicated to ASC biology, additional sections will be devoted to ASCs for stem cell therapy and regenerative medicine, as well as ASCs in tissue engineering applications
Download or read book Methods in Molecular Biology Adipose tissue protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Thermogenic Fat written by Irfan J. Lodhi and published by Springer Nature. This book was released on 2023-04-19 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, and mass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Thermogenic Fat: Methods and Protocols aims to be comprehensive guide for researchers in the field.
Download or read book Adipose Derived Stem Cells written by Jeffrey M. Gimble and published by Humana Press. This book was released on 2011-08-24 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular BiologyTM series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.
Download or read book Adipose Tissue and Adipokines in Health and Disease written by Giamila Fantuzzi and published by Springer Science & Business. This book was released on 2014-04-25 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of adipose tissue biology has been expanding at a very rapid pace in the last few years. Numerous advances have been made since publication of the first edition of this book, in terms of basic adipocyte biology, understanding of the determinants of obesity, distribution of body fat and weight loss, as well as the mechanisms linking excess adiposity to various co-morbidities. Adipose Tissue and Adipokines in Health and Disease, Second Edition contains updated chapters from the previous volume but as the field has evolved, some areas covered in the first edition have been refocused to address the new knowledge. The volume is divided in four sections: the first two deal with basic adipose tissue and adipokine biology, while the last two address the problem of obesity and alterations in adipose tissue function from an epidemiological and clinical standpoint. The chapters are written by experts in their fields and include the most up to date scientific information. Adipose Tissue and Adipokines in Health and Disease, Second Edition is a useful resource for physicians interested in adipose tissue biology and basic scientists who want to know more about applied aspects of the field. The book targets endocrinologists, residents and fellows, internists, nutritionists and general practitioners who are exposed to an ever-expanding obese population.
Download or read book Organ on Chip Systems Integrating Human Adipose Tissues written by Julia Rogal and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adipose tissue constitutes about one fourth of a healthy adult human's body mass and is involved in a large variety of (patho-)physiological processes. Especially in the era of 'diabesity', a thorough understanding of human adipose tissue has become more important than ever. Yet, research on human adipose biology is hampered by a lack of predictive model systems. Even though many valuable insights could be gained from animal models, they often fall short of predicting human physiology. Then again, unusual characteristics of mature adipocytes, such as buoyancy, fragility, and large size, make conventional cell culture approaches challenging. In recent years, organ-on-chip (OoC) technology has emerged from a synergy of tissue engineering and microfluidics approaches. OoC systems integrate engineered tissues into physiological microenvironments supplied by a vasculature-like perfusion. Yet even though OoC technology is thriving regarding other organ systems, there has only been very little focus on adipose tissue so far. Hence, the objectives of this thesis were to design, develop and characterize adipose tissue-on-chip models. To achieve this, designs, biomaterials and fabrication approaches were developed leading to three generations of microfluidic platforms specifically tailored to the needs of human adipose tissues. Moreover, protocols and logistics for sourcing, isolating, and utilizing almost all adipose tissue cell types from one donor were established. Together, this enabled the generation of white and beige adipose tissues (WAT and bAT, respectively) on chip either by injecting mature adipose cell types (in case of WAT) or by inducing adipogenesis on chip (in case of bAT). Along the way towards a highly complex, immunocompetent autologous model integrating almost all adipose-associated cell types, a mix-and-match strategy was established allowing for a flexible combination of cellular modules to fit-for-purpose models serving a specific scientific question. Moreover, a toolbox of readout methods was compiled that enabled a comprehensive characterization of on-chip adipose tissue structure and function, demonstrating functional on-chip WAT culture times beyond one month. Case studies on compound screening and immune responses highlighted the models' suitability as tools for target identification in drug discovery or for studies on immunometabolism. All in all, the developed models hold great potential for mechanistic studies on adipose tissue biology or disease modelling in the context of obesity and diabetes, as well as for personalized or precision medicine due to its fully autologous character.
Download or read book Brown Adipose Tissue written by Alexander Pfeifer and published by Springer. This book was released on 2019-04-02 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: The main focus of this book is on brown adipose tissue and its metabolic function. The book provides a timely update on the latest research and shows where the field is heading. Brown adipose tissue (BAT) dissipates energy and has received considerable attention in the last few years, having been re-discovered in adult humans in 2007/9. Moreover, BAT might offer a target for novel therapies to address obesity, a health condition that has reached pandemic dimensions.
