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Book Protocols in Molecular Parasitology

Download or read book Protocols in Molecular Parasitology written by John E. Hyde and published by Taylor & Francis US. This book was released on 1993-08-11 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.

Book Protocols in Molecular Parasitology

Download or read book Protocols in Molecular Parasitology written by John E. Hyde and published by Humana Press. This book was released on 1993-08-11 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols in Molecular Parasitology offers a wide range of experi mental protocols, each written by experts, to research workers interested in exploring the molecular aspects of parasitology. This is a field that has expanded enormously in scope and potential over the last decade, although it is as yet significantly underrepresented in the literature out side of academic journals. The need for manuals such as this one stems in part from the increasing difficulty of reproducing experimental tech niques from reports in standard journal papers, where pressures on space usually result in highly abbreviated descriptions. The "Notes" sections of the following chapters are particularly aimed at counteracting this problem. Since the emphasis of the book is on aspects of molecular analysis, it was possible to devote only a limited amount of space to describing protocols for culture and isolation of the primary parasitic material. Therefore, of the numerous parasitic systems that are currently being investigated in laboratories around the world, only techniques for c- turing the "big five" are described here, since they collectively account for the great bulk of research in this field. It is also possible that some minor front-end modifications to certain protocols may be necessary, depending on exactly which parasite is being studied.

Book Malaria

    Book Details:
  • Author : Robert Ménard
  • Publisher : Humana Press
  • Release : 2016-08-23
  • ISBN : 9781493959587
  • Pages : 626 pages

Download or read book Malaria written by Robert Ménard and published by Humana Press. This book was released on 2016-08-23 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory researchers.

Book Parasite Genomics

    Book Details:
  • Author : Luis M. de Pablos
  • Publisher : Humana
  • Release : 2022-08-11
  • ISBN : 9781071616833
  • Pages : 336 pages

Download or read book Parasite Genomics written by Luis M. de Pablos and published by Humana. This book was released on 2022-08-11 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book provides a comprehensive series of innovative research techniques and methodologies applied to the parasite genomics research area, all applying different approaches to analyzing parasite genomes and furthering the study of genetic complexity and the mechanisms of regulation. Beginning with chapters on novel sequencing and the bioinformatics pipeline, the volume continues by exploring diagnostic approaches using genomic tools, host-parasite interactions, as well as the genomics of parasite-derived extracellular vesicles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Parasite Genomics: Methods and Protocols creates a detailed picture of genomic approaches for researchers seeking a better understanding of characterizing parasite nucleic acid content.

Book Parasite Genomics Protocols

    Book Details:
  • Author : Sara E. Melville
  • Publisher : Springer Science & Business Media
  • Release : 2008-02-02
  • ISBN : 1592597939
  • Pages : 454 pages

Download or read book Parasite Genomics Protocols written by Sara E. Melville and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: Parasitic diseases remain a major health problem throughout the world, for both humans and animals. For many of us, our technologically advanced lifestyle has decreased the prevalence and transmission of parasitic diseases, but for the majority of the world’s population, they are ever present in homes, domestic animals, food, or the environment. The study of parasites and parasitic disease has a long and distinguished history. In some cases, it has been driven by the great importance of the presence of the parasite to the community, for example, those that affect our livestock. In other cases, it is clear that applied research has suffered for lack of funding because the parasite affects people with few resources, such as the rural poor in resource-poor countries. These instances include the so-called “neglected diseases,” as defined by the World Health Organization (WHO). Parasites have complicated life cycles, and a thorough understanding of the unique characteristics of a particular parasite species is vital in attempts to avoid, prevent, or cure infection or to alleviate symptoms. Of course, the biological characteristics that each parasite has developed to aid survival and transmission, to avoid destruction by the immune system, and to adapt to a changing environment are of lasting fascination to basic biologists as well. The elegance of these biological systems has ensured that the study of protozoan and metazoan parasites also remains an active field of research in countries where the diseases are not a threat to the population.

Book Cryptosporidium

Download or read book Cryptosporidium written by Jan R. Mead and published by . This book was released on 2019 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.

Book Protocols used in Molecular Biology

Download or read book Protocols used in Molecular Biology written by Sandeep Singh and published by Bentham Science Publishers. This book was released on 2020-01-23 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Book Current Protocols in Molecular Biology

Download or read book Current Protocols in Molecular Biology written by Frederick M. Ausubel and published by Current Protocols. This book was released on 2003-12 with total page 1600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Protocols in Molecular Biology (CPMB), the first in the Current Protocols family of laboratory manuals, is the international standard by which all other laboratory manuals are judged. Originally published in 1987, CPMB now boasts of three looseleaf volumes (soon to expand to four volumes). Basic methods for DNA preparation and isolation, library screening, and sequencing have been joined by more advanced procedures detailing DNA-protein interactions, yeast manipulation, and phosphorylation analyses. From basics to the cutting edge, CPMB is the resource you need for successful experiments. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Quarterly updates, which are filed into the looseleaf, keep the set current with the latest developments in molecular biology methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.

Book Short Protocols in Molecular Biology

Download or read book Short Protocols in Molecular Biology written by Frederick M. Ausubel and published by . This book was released on 1992 with total page 852 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Trypanosomatids

Download or read book Trypanosomatids written by Paul A. M. Michels and published by . This book was released on 2020 with total page 823 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases. Chapters 14, 15, 16, 23, 24, 30, and 48 are open access under a CC BY 4.0 license.

Book Current Protocols in Molecular Biology

Download or read book Current Protocols in Molecular Biology written by Frederick M. Ausubel and published by Current Protocols. This book was released on 1993-04 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Protocols in Molecular Biology on CD-ROM (CPMB on CD) is the international standard by which all other laboratory manuals are judged. CPMB on CD contains the complete contents of the corresponding three-volume looseleaf (soon to expand to four volumes) with the added benefits of a quick and intuitive search engine and user interface. Basic methods for DNA preparation and isolation, library screening, and sequencing have been joined by more advanced procedures detailing DNA-protein interactions, yeast manipulation, and phosphorylation analyses. From basics to the cutting edge, CPMB on CD is the resource you need for successful experiments. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Quarterly update discs keep the set current with the latest developments in molecular biology methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.

Book Molecular Parasitology

    Book Details:
  • Author : John E. Hyde
  • Publisher : Chapman & Hall
  • Release : 1990-01-01
  • ISBN : 9780442308247
  • Pages : 302 pages

Download or read book Molecular Parasitology written by John E. Hyde and published by Chapman & Hall. This book was released on 1990-01-01 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book T  Cruzi Infection

Download or read book T Cruzi Infection written by Karina Andrea Gómez and published by . This book was released on 2019 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Schistosoma Mansoni

    Book Details:
  • Author : David J. Timson
  • Publisher : Humana
  • Release : 2021-06-09
  • ISBN : 9781071606377
  • Pages : 265 pages

Download or read book Schistosoma Mansoni written by David J. Timson and published by Humana. This book was released on 2021-06-09 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Parasites and Molecular Biology

Download or read book Parasites and Molecular Biology written by A. J. G. Simpson and published by . This book was released on 1986 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Host Pathogen Interactions

Download or read book Host Pathogen Interactions written by Steffen Rupp and published by Humana Press. This book was released on 2008-10-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research. In Host-Pathogen Interactions: Methods and Protocols, top experts examine the relationship between the host and the pathogen, crucial in the outcome of an infection and the establishment of disease or asymptomatic, commensal colonization by organisms. The step-by-step laboratory methods and protocols of this volume study host-pathogen interaction, with a focus on fungal, bacterial and parasitic pathogens, at a molecular level in order to reveal the mechanisms of infection and to identify the vulnerabilities of the pathogen of interest. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters feature brief subject introductions, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Host-Pathogen Interactions: Methods and Protocols serves as an easy entry point for all those investigating the factors responsible for the pathogenicity of microorganisms.