Download or read book Flavoprotein Protocols written by Steven K. Chapman and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
Download or read book Methods in Molecular Biology Flavoprotein protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Flavins and Flavoproteins written by Stefan Weber and published by Humana. This book was released on 2016-09-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins. These include review style methods and protocols to exemplify the variety, the power and the success of modern techniques and methods in application to flavoproteins. Part I of this Volume covers general properties, syntheses and applications of free flavins as well as its analogs and flavoproteins. Part II covers characterizations of flavins and flavoproteins using modern experimental techniques as well as theoretical methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Flavins and Flavoproteins: Methods and Protocols aids scientists in continuing to tackle the countless questions that need to be answered to more fully comprehend the vast diversity and specificity of flavin-governed biological processes.
Download or read book Flavoprotein Protocols written by Steven K. Chapman and published by Humana Press. This book was released on 1999-07-01 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
Download or read book Bioconjugation Protocols written by Christof M. Niemeyer and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are a number of outstanding volumes that provide a comprehensive overview of bioconjugation techniques. However, many of the conventional approaches to the synthesis of chemically modified protein conjugates lack efficient means to control the stoichiometry of conjugation, as well as the s- cific site of attachment of the conjugated moiety. Moreover, the recent dev- opments in microarray technologies as well as in nanobiotechnology—a novel field of research rapidly evolving at the crossroads of physics, chemistry, b- technology, and materials science—call for a summary of modern bioconjugation strategies to overcome the limitations of the classical approaches. Bioconjugation Protocols: Methods and Strategies is intended to provide an update of many of the classic techniques and also to introduce and summarize newer approaches that go beyond the pure biomedical applications of bioconjugation. The purpose of Bioconjugation Protocols: Methods and Str- egies is therefore to provide instruction and inspiration for all those scientists confronting the challenges of semisynthesizing functional biomolecular reagents for a wide variety of applications ranging from novel biomedical diagnostics, to therapeutics, to biomaterials. Part I contains seven protocols for the preparation of protein conjugates.
Download or read book Short Protocols in Molecular Biology written by Frederick M. Ausubel and published by . This book was released on 2002 with total page 838 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level Expanded to 2 volumes.
Download or read book Protocols used in Molecular Biology written by Sandeep Singh and published by Bentham Science Publishers. This book was released on 2020-01-23 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
Download or read book Short Protocols in Molecular Biology written by Frederick M. Ausubel and published by Current Protocols. This book was released on 1999-05-03 with total page 1134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Short Protocols in Molecular Biology Fourth Edition The Desktop Guide to Your Lab Edited by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl Providing condensed descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition of the classic laboratory manual thoroughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated-Short Protocols in Molecular Biology, Fourth Edition offers both. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in cutting-edge techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection have also benefited from the updating and reflect the methods currently used in leading research facilities around the world. New topics to this edition include: * Informatics for Molecular Biologists * Analysis of Protein Interactions * Epitope Tagging * Mathematics and Statistics for Molecular Biologists Short Protocols in Molecular Biology, Fourth Edition is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of molecular biology methods.
Download or read book Current Protocols in Molecular Biology written by Frederick M. Ausubel and published by Current Protocols. This book was released on 2003-12 with total page 1600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Protocols in Molecular Biology (CPMB), the first in the Current Protocols family of laboratory manuals, is the international standard by which all other laboratory manuals are judged. Originally published in 1987, CPMB now boasts of three looseleaf volumes (soon to expand to four volumes). Basic methods for DNA preparation and isolation, library screening, and sequencing have been joined by more advanced procedures detailing DNA-protein interactions, yeast manipulation, and phosphorylation analyses. From basics to the cutting edge, CPMB is the resource you need for successful experiments. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Quarterly updates, which are filed into the looseleaf, keep the set current with the latest developments in molecular biology methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.
Download or read book Methods in Molecular Biology Membrane protein protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Complement methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Chaperonin protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Crystallographic methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomembrane Protocols written by Graham, John and published by . This book was released on 1993 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Immunochemical protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Ribozyme protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Immunocytochemical methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
