Download or read book Connexin Methods and Protocols written by Roberto Bruzzone and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: Direct cell–cell communication is a common property of multicellular organisms that is achieved through membrane channels which are organized in gap junctions. The protein subunits of these intercellular channels, the connexins, form a multigene family that has been investigated in great detail in recent years. It has now become clear that, in different tissues, connexins speak several languages that control specific cellular functions. This progress has been made possible by the availability of new molecular tools and the improvement of basic techniques for the study of membrane channels, as well as by the use of genetic approaches to study protein function in vivo. More important, connexins have gained visibility because mutations in some connexin genes have been found to be linked to human genetic disorders. Connexin Methods and Protocols presents in detail a collection of te- niques currently used to study the cellular and molecular biology of connexins and their physiological properties. The field of gap junctions and connexin research has always been characterized by a multidisciplinary approach c- bining morphology, biochemistry, biophysics, and cellular and molecular biology. This book provides a series of cutting-edge protocols and includes a large spectrum of practical methods that are available to investigate the fu- tion of connexin channels. Connexin Methods and Protocols is divided into three main parts.
Download or read book Methods in Molecular Biology Connexin methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Connexin Hemichannels written by Fabio Mammano and published by Springer Nature. This book was released on with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Connexin Methods and Protocols written by Roberto Bruzzone and published by Humana Press. This book was released on 2000-12-01 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: Direct cell–cell communication is a common property of multicellular organisms that is achieved through membrane channels which are organized in gap junctions. The protein subunits of these intercellular channels, the connexins, form a multigene family that has been investigated in great detail in recent years. It has now become clear that, in different tissues, connexins speak several languages that control specific cellular functions. This progress has been made possible by the availability of new molecular tools and the improvement of basic techniques for the study of membrane channels, as well as by the use of genetic approaches to study protein function in vivo. More important, connexins have gained visibility because mutations in some connexin genes have been found to be linked to human genetic disorders. Connexin Methods and Protocols presents in detail a collection of te- niques currently used to study the cellular and molecular biology of connexins and their physiological properties. The field of gap junctions and connexin research has always been characterized by a multidisciplinary approach c- bining morphology, biochemistry, biophysics, and cellular and molecular biology. This book provides a series of cutting-edge protocols and includes a large spectrum of practical methods that are available to investigate the fu- tion of connexin channels. Connexin Methods and Protocols is divided into three main parts.
Download or read book Gap Junction Protocols written by Mathieu Vinken and published by Methods in Molecular Biology. This book was released on 2018-06-04 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Corrected statement of responsibility may be: edited by ...
Download or read book Two Hybrid Systems written by Paul N. MacDonald and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast) to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problems of artifacts and false positives and for identifying the interacting partners in important biological systems, including the Smad and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly. Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques.
Download or read book Bioconjugation Protocols written by Christof M. Niemeyer and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are a number of outstanding volumes that provide a comprehensive overview of bioconjugation techniques. However, many of the conventional approaches to the synthesis of chemically modified protein conjugates lack efficient means to control the stoichiometry of conjugation, as well as the s- cific site of attachment of the conjugated moiety. Moreover, the recent dev- opments in microarray technologies as well as in nanobiotechnology—a novel field of research rapidly evolving at the crossroads of physics, chemistry, b- technology, and materials science—call for a summary of modern bioconjugation strategies to overcome the limitations of the classical approaches. Bioconjugation Protocols: Methods and Strategies is intended to provide an update of many of the classic techniques and also to introduce and summarize newer approaches that go beyond the pure biomedical applications of bioconjugation. The purpose of Bioconjugation Protocols: Methods and Str- egies is therefore to provide instruction and inspiration for all those scientists confronting the challenges of semisynthesizing functional biomolecular reagents for a wide variety of applications ranging from novel biomedical diagnostics, to therapeutics, to biomaterials. Part I contains seven protocols for the preparation of protein conjugates.
Download or read book Molecular Chaperones written by Stuart K. Calderwood and published by Humana Press. This book was released on 2011-09-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular BiologyTM series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.
Download or read book Short Protocols in Molecular Biology written by Frederick M. Ausubel and published by . This book was released on 2002 with total page 838 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level Expanded to 2 volumes.
Download or read book Methods in Molecular Biology Protein structure prediction methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Two hybrid systems Methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology High throughput screening methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Bioinformatics methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Gene transfer and expression protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Protocols in human molecular genetics written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Complement methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Adhesion protein protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
