Download or read book Methods in Molecular Biology and Protein Chemistry written by Brenda D. Spangler and published by Wiley. This book was released on 2002-06-14 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ein praxisorientierter Leitfaden für Studenten im Hauptstudium. Vermittelt werden aktuelle Techniken der Molekularbiologie und Biochemie als fester Bestandteil eines Forschungsprojektes. Auf diese Weise kann der Lernende die Auswirkung der Gentechnik anhand der praktischen Anwendung von nassanalytischen biochemischen Verfahren erkennen. Mit Computer-Tutorials für Sequenzierung, Genomik und Molekülmodellierung. Mit Tipps und Hinweisen zur Sichtung der Primärliteratur, zu mündlichen Berichten, zur Protokollierung des Labortagebuchs und zum Verfassen von Berichten. Mit einer Liste von amerikanischen und europäischen kommerziellen Anbietern von Reagenzien. Verständlich und anschaulich geschrieben. Ein unverzichtbares Lehrbuch für fortgeschrittene Studenten und Doktoranden. "Methods in Molecular Biology" ist jedoch NICHT geeignet für aktive Forscher auf der Suche nach den neuesten hochdifferenzierten Verfahren.
Download or read book Advances in Protein Molecular and Structural Biology Methods written by Timir Tripathi and published by Academic Press. This book was released on 2022-01-14 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. Presents a thorough overview of the latest and emerging methods and technologies for protein study Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy Includes computational and machine learning methods Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins
Download or read book Methods in Molecular Biology and Protein Chemistry written by Brenda D. Spangler and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Protein Biochemistry written by Harald Tschesche and published by Walter de Gruyter. This book was released on 2011-12-23 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.
Download or read book Techniques in Protein Chemistry written by and published by Elsevier. This book was released on 1995-04-13 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques in Protein Chemistry VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: Protein sequencing and amino acid analysis Mass spectral analysis of peptides and proteins Posttranslational processing High-sensitivity protein and peptide separations Protein folding and NMR Analysis of protein interactions Protein design and engineering Techniques in Protein Chemistry VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: Protein sequencing and amino acid analysis Mass spectral analysis of peptides and proteins Posttranslational processing High-sensitivity protein and peptide separations Protein folding and NMR Analysis of protein interactions Protein design and engineering
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Techniques in Protein Chemistry written by and published by Elsevier. This book was released on 1997-07-21 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: Praise for the Series:"The mainly sharp scientific focus of this set of snapshots is a credit to both the contricutors and the editorial team."--Biotechnology and Applied Biochemistry Techniques in Protein Chemistry VIII is the latest volume in this successful series. As a valuable bench-top reference tool for protein chemists, the ten section sof the book are divided by subject area to show the reader which techniques are currently applied to particular problems in protein science. This approach reflects current trends in which specific instruments and methodologies are used in several different areas. * * The book features the latest advances in protein chemistry methodologies in the following areas:* Protein sequencing and amino acid analysis* Mass spectral analysis of peptides and proteins* Posttranslational processing* High-sensitivity protein and peptide separations* Protein folding and NMR* Functional domain analysis* Protein design and engineering* Three-dimensional protein structure
Download or read book Physical Principles and Techniques of Protein Chemistry Part C written by Sydney Leach and published by Elsevier. This book was released on 2012-12-02 with total page 639 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry.
Download or read book Protein Purification Protocols written by Paul Cutler and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Download or read book Physical Principles and Techniques of Protein Chemistry Part B written by Sydney Leach and published by Elsevier. This book was released on 2012-12-02 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.
Download or read book Techniques in Molecular Biology written by John M. Walker and published by . This book was released on 1983 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Research in Protein Chemistry written by Villafranc and published by Elsevier. This book was released on 2012-12-02 with total page 635 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Research in Protein Chemistry: Techniques, Structure, and Function focuses on the techniques and methods used for determining the structure and function of proteins. Topics covered range from protein folding and stability to catalysis by chimeric proteins, amino acid and peptide analysis, applications of mass spectrometry to peptide and protein analysis, and protein sequencing. This book is divided into six sections encompassing 55 chapters. The first chapter describes a novel method for protein hydrolysis by means of microwave irradiation that uses Teflon-Pyrex tubes. This is followed by a discussion of the application of high performance capillary electrophoresis to the analysis of amino acids. The sections that follow focus on mass spectrometric methods, protein sequencing, and capillary electrophoresis as well as protein stability, chimeric proteins and enzyme modifications, and protein structure prediction. The crystal structure of human interleukin-1alpha, the acid-denatured states of proteins, solubility of recombinant proteins expressed in Escherichia coli, and catalysis by chimeric proteins are considered. The reader is also introduced to peptide mapping and internal sequencing of proteins from acrylamide gels, new approaches to covalent sequence analysis, alkaline denaturation of hemoglobin, and measurements of disulfide bond stabilities in protein folding intermediates. Students and researchers interested in protein chemistry will find this book extremely helpful.
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1099 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Download or read book Techniques in Protein Chemistry written by Tony Hugli and published by Elsevier. This book was released on 2012-12-02 with total page 631 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques in Protein Chemistry compiles reports of methods and techniques presented at the second symposium of the Protein Society in August 1988. This book includes methods and applications in protein sequencing, advanced applications of mass spectrometry and nuclear magnetic resonance technology, limitations of amino acid microanalysis, and advances in high-performance liquid chromatography. The structure of synthetic test peptide-3 (STP-3), a peptide designed to test the analytical limits of current technology in the field of protein chemistry is also elaborated. This publication is suited for chemists and researchers conducting work on the analytical techniques available for the molecular characterization of proteins.
Download or read book Protein NMR Techniques written by Alexander Shekhtman and published by Humana Press. This book was released on 2016-05-01 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: In its expanded third edition, this Methods in Molecular Biology volume offers techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing, materials lists, step-by-step protocols, troubleshooting tips and more."
Download or read book Techniques in Protein Chemistry III written by Ruth Hogue Angeletti and published by Academic Press. This book was released on 2014-06-28 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques in Protein Chemistry III compiles papers presented at the Fifth Protein Society Symposium in Baltimore on June 22-26, 1991. This book discusses the protein and peptide recovery from PVDF membranes; high-sensitivity peptide mapping utilizing reversed-phase microbore and microcolumn liquid chromatography; and capillary electrophoresis for preparation of peptides and direct determination of amino acids. The TFMSA/TFA cleavage in t-Boc peptide synthesis; applications of automatic PTC amino acid analysis; and identification of O-glycosylation sites with a gas phase sequencer are also elaborated. This text likewise covers the conformational stability of the molten globule of cytochrome c and role of aqueous solvation in protein folding. This publication is useful to students and researchers interested in methods and research approaches on protein chemistry.
Download or read book Chemical Protein Synthesis written by Xuechen Li and published by Springer Nature. This book was released on 2022-06-27 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chemical Protein Synthesis aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
