Download or read book Membrane Protein Assembly written by Gunnar von Heijne and published by R. G. Landes. This book was released on 1997 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Membrane Protein Structure and Function Characterization written by Jean-Jacques Lacapere and published by Humana Press. This book was released on 2017-08-10 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Protein Targeting Transport and Translocation written by Ross Dalbey and published by Elsevier. This book was released on 2002-04-09 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Targeting, Transport, and Translocation presents an in-depth overview on the topic of protein synthesis, covering all areas of protein science, including protein targeting, secretion, folding, assembly, structure, localization, quality control, degradation, and antigen presentation. Chapters also include sections on the history of the field as well as summary panels for quick reference. Numerous color illustrations complement the presentation of material. This book is an essential reference for anyone in biochemistry and protein science, as well as an excellent textbook for advanced students in these and related fields. Basic principles and techniques Targeting adn sorting sequences Protein export in bacteria Membrane protein integration into ER and bacterial membranes Protein translocation across the ER Disulfide bond formation in prokaryotes and eukaryotes Quality control in the export pathway Import of proteins into organelles The secretory pathway Vesicular transport Spectacular color throughout
Download or read book The BAM Complex written by Susan Buchanan and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
Download or read book Protein Export and Membrane Biogenesis written by R.E. Dalbey and published by Elsevier. This book was released on 1995-08-31 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: The incentive for putting together Volume 4 of this series was to review the wealth of new information that has become available in prokaryotic organisms in protein export and membrane biogenesis. Just in the last several years, protein translocation has now been efficiently reconstituted using defined components and the mechanism by which proteins are moved across membrane bilayers is now being examined at a higher resolution. In addition, because of a new technical breakthrough using osmolytes, it is now possible to reconstitute a number of channel proteins, ATPase, receptors, and transporters. In many cases, it is possible to successfully predict the membrane topology of these types of proteins using both "hydrophobicity analysis" and the "positive inside" rule. In this volume, two chapters focus on protein translocation across membranes (Biochemical Analyses of Components Comprising the Protein Translocation Machinery of E. Coli; Protein Translocation Genetics), while several others on how proteins assemble into the ineer membrane of E. Coli (Membrane Protein Assembly; Membrane Insertion of Small Proteins: Evolutionary and Functional Aspects; Pigment-Protein Complex Assembly in Rhodobacter sphaeroides and Rhodobacter Capsulatus). Other sections review recent progress on transporters (Identification and Reconstitution of Anion Exchange Mechanisms in Bacteria; Helic Packing in the C-Terminal Half of Lactose Permease) and signal transduction (Mechanism of Transmembrane Signaling in Osmoregulation) as well as the assembly of prints into the outer membrane (Export and Assembly of Outer Membrane Proteins in E. coli). Although the emphasis of the book is on proteins, the role of phospholipids in controlling various cell surface processes is reviewed (Role of Phospholipids in coli Cell Function). I should point out the reason for the rapid progress in bacteria research is because of the possibility to apply biochemistry and genetics in this organism.
Download or read book Membrane Protein Structure written by Stephen H White and published by Springer. This book was released on 2013-05-27 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of receptors, ion channels, and other membrane proteins require a solid understanding of the structural principles of these important biomolecules. Membrane protein structure is, however, a very challenging field. The structures of only three types of transmembrane proteins have been determined to moderate or high resolution during the last two decades, a period during which the amino acid sequences of hundreds, if not thousands, of membrane proteins have been reported. As a result, the creation of structural models to serve as guides for studies of receptors, channels, and other membrane proteins has become crucially important. This book has been assembled in order to share the experiences and findings of expert researchers in protein structure and structure-prediction methods as well as membrane biophysics and lipid physical chemistry, whose work establishes the basis for the development of suitable model structures. The reviews presented here emphasize fundamental ideas and provide an entry to the diverse and complex literature. The four major sections deal with the general nature of the membrane protein structure problem, biochemical and molecular biological approaches to protein topology, direct structural methods, and model and physicochemical approaches. The work will be of interest to physiologists, cellular and molecular biologists, biophysicists, and biochemists working on the function of membrane proteins such as receptors, ion channels, and transporters, as well as senior graduate students and independent investigators.
Download or read book Studies on the Membrane Protein Assembly in Escherichia Coli written by Guoqing Cao and published by . This book was released on 1995 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Local Structural Motifs Involved in Helical Membrane Protein Assembly and Dynamic Function written by Ilan Samish and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Lipid and Membrane Traffic written by North Atlantic Treaty Organization. Scientific Affairs Division and published by IOS Press. This book was released on 2000 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text concentrates on the following specific topics: the dynamic character of lipids and proteins in biological membranes; the existence of specific domains in membranes including their visualisation; the molecular mechanisms of intracellular transport of membrane constituents and the involvement of lipid-protein interactions in these processes; protein assembly; structure and folding and transport through membranes; and the intracellular sorting and targeting of individual membrane components as well as different organelles."
Download or read book A Study of Various Components Influencing Outer Membrane Protein Assembly in Escherichia Coli K 12 written by Michael Wilson Laird and published by . This book was released on 1996 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Boundaries written by Stephen H White and published by Garland Science. This book was released on 2021-12-31 with total page 565 pages. Available in PDF, EPUB and Kindle. Book excerpt: The central themes of Cell Boundaries concern the structural and organizational principles underlying cell membranes, and how these principles enable function. By building a biological and biophysical foundation for understanding the organization of lipids in bilayers and the folding, assembly, stability, and function of membrane proteins, the book aims to broaden the knowledge of bioscience students to include the basic physics and physical chemistry that inform us about membranes. In doing so, it is hoped that physics students will find familiar territory that will lead them to an interest in biology. Our progress toward understanding membranes and membrane proteins depends strongly upon the concerted use of both biology and physics. It is important for students to know not only what we know, but how we have come to know it, so Cell Boundaries endeavours to bring out the history behind the central discoveries, especially in the early chapters, where the foundation is laid for later chapters. Science is far more interesting if, as students, we can appreciate and share in the adventures—and misadventures—of discovering new scientific knowledge. Cell Boundaries was written with advanced undergraduates and beginning graduate students in the biological and physical sciences in mind, though this textbook will likely have appeal to researchers and other academics as well. Highlights the history of important central discoveries Early chapters lay the foundation for later chapters to build on, so knowledge is amassed High-quality line diagrams illustrate key concepts and illuminate molecular mechanisms Box features and spreads expand on topics in main text, including histories of discoveries, special techniques, and applications
Download or read book Integral Membrane Protein Assembly and Stability written by Amanda L. Stouffer and published by . This book was released on 2006 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural Biochemistry of the Tol Pal Inner Membrane Protein Assembly written by Cara Press and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Use of Beta lactamase Fusions to Study Membrane Protein Assembly and Protein Export in Escherichia Coli written by Yianbiao Zhang and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Use of Beta lactamase Fusions to Study Membrane Protein Assembly and Protein Export in Escherichia Coli written by and published by . This book was released on 1991 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A genetic system for directly expressing proteins in E. coli and assessing their ability to insert into the bacterial cytoplasmic membrane was developed, consisting of a direct expression vector, and a mature P-lactamase cassette plasmid for generating translational fusions of P-lactamase to the bacterially expressed proteins. This system was used to analyse the fate of three different eukaryotic plasma membrane proteins in E. coli. The ampicillin resistance/sensitivity phenotypes of the P-lactamase fusion proteins revealed that the P-subunit of the sheep-kidney Na,K-ATPase, a class II membrane protein, inserts into the cytoplasmic membrane of E. coli, with the same topology as in its native membrane. The Alzheimer's disease amyloid A4 protein precursor, a class I membrane protein, was also found to insert into the bacterial cytoplasmic membrane, and, although preliminary, the results were compatible with its correct topological insertion into the bacterial cytoplasmic membrane. In contrast, the P2-adrenergic receptor, a complex membrane protein that has an extracellular amino-terminal domain and seven transmembrane segments, was found to misinsert into the E. coli cytoplasmic membrane. A plasmid vector for cloning sequences able to function as cytoplasmic membrane translocators of P-lactamase in E. coli was also developed. Short DNA fragments of essentially random sequence were cloned, and recombinants with functional translocators were directly selected. The spectrum of random sequences able to function as bacterial cytoplasmic membrane translocators suggests that the hydrophobicity requirements for translocation across the bacterial cytoplasmic membrane and yeast endoplasmic reticulum membrane may be more similar than previously assumed.In the above studies new and widely applicable uses of P-lactamase fusion technology have been described. As applied in this thesis they highlight similarities and differences between the mechanisms by which proteins assemble into, or are translocated across, the bacterial cytoplasmic membrane and the eukaryotic endoplasmic reticulum membrane.
Download or read book Study of Bacteriorhodopsin in a Controlled Lipid Environment written by Vivien Yeh and published by Springer. This book was released on 2018-10-26 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.
