Download or read book Membrane Chromatography of Biological Nanoparticles written by Mark Raymond Etzel and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Nanoparticle Interactions written by Masoud Rahman and published by Springer Science & Business Media. This book was released on 2013-06-24 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the fabrication of nanomaterials and exploration of their properties have attracted the attention of various scientific disciplines such as biology, physics, chemistry, and engineering. Although nanoparticulate systems are of significant interest in various scientific and technological areas, there is little known about the safety of these nanoscale objects. It has now been established that the surfaces of nanoparticles are immediately covered by biomolecules (e.g. proteins, ions, and enzymes) upon their entrance into a biological medium. This interaction with the biological medium modulates the surface of the nanoparticles, conferring a “biological identity” to their surfaces (referred to as a “corona”), which determines the subsequent cellular/tissue responses. The new interface between the nanoparticles and the biological medium/proteins, called “bio-nano interface,” has been very rarely studied in detail to date, though the interest in this topic is rapidly growing. In this book, the importance of the physiochemical characteristics of nanoparticles for the properties of the protein corona is discussed in detail, followed by comprehensive descriptions of the methods for assessing the protein-nanoparticle interactions. The advantages and limitations of available corona evaluation methods (e.g. spectroscopy methods, mass spectrometry, nuclear magnetic resonance, electron microscopy, X-ray crystallography, and differential centrifugal sedimentation) are examined in detail, followed by a discussion of the possibilities for enhancing the current methods and a call for new techniques. Moreover, the advantages and disadvantages of protein-nanoparticle interaction phenomena are explored and discussed, with a focus on the biological impacts.
Download or read book Nanoparticle Protein Corona written by Ashutosh Kumar and published by Royal Society of Chemistry. This book was released on 2019-08-02 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoparticles have numerous biomedical applications including drug delivery, bone implants and imaging. A protein corona is formed when proteins existing in a biological system cover the nanoparticle surface. The formation of a nanoparticle–protein corona, changes the behaviour of the nanoparticle, resulting in new biological characteristics and influencing the circulation lifetime, accumulation, toxicity, cellular uptake and agglomeration. This book provides a detailed understanding of nanoparticle–protein corona formation, its biological significance and the factors that govern the formation of coronas. It also explains the impact of nanoparticle–protein interactions on biological assays, ecotoxicity studies and proteomics research. It will be of interest to researchers studying the application of nanoparticles as well as toxicologists and pharmaceutical chemists.
Download or read book Chromatographic and Membrane Processes in Biotechnology written by C.A. Costa and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation processes in biotechnology are of increasing industrial importance since they entail the major costs of bioprocessing especially when high purity is required. Chromatography and membranes are two of the most important technologies used for direct treatment of fermentation broths as well as for high resolution steps in product purification. The theoretical foundations of chromatographic and membrane processes are well understood for the case of small molecules. Nevertheless there is a need to adapt and further develop that knowledge to the processing of large biological molecules. This is being achieved with the contribution of other areas like molecular biology and materials science. The objective of this NATO Advanced Study Institute is to present an updated treatment of the fundamentals of chromatographic and membrane processes with special relevance in bioprocessing.This volume collects the lectures presented at this Institute. The lectures are arranged in five chapters. Chapter I deals with chromatographic processes covering topics like equilibrium, kinetics and contacting devices. Membrane processes and some applications in biotechnology are treated in chapter 2. Chapter 3 is devoted to affinity chromatographic and membrane processes. Chapter 4 considers the current developments on chromatographic supports and membranes both from the constitutive materials and form points of view. Scale-up, optimization and reaction/separation integration are the topics covered in chapter 5. We are very grateful to all lecturers and participants that made possible this Institute. Financial support from NATO Scientific Affairs Division, INIC, JNICT, FLAD, University of Ac;ores and DRT Ac;ores is gratefully acknowledged.
Download or read book Protein Chromatography written by Sinéad T Loughran and published by Springer Nature. This book was released on 2023-10-01 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.
Download or read book Separation of Nanometer sized Biological Particles Using Membrane Chromatography written by Joachim Fischer and published by . This book was released on 2001 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Membrane Separations written by Anil K. Pabby and published by CRC Press. This book was released on 2023-12-22 with total page 1334 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of the Handbook of Membrane Separations: Chemical, Pharmaceutical, Food, and Biotechnological Applications provides a comprehensive discussion of membrane applications. Fully updated to include the latest advancements in membrane science and technology, it is a one-of-its-kind overview of the existing literature. This fully illustrated handbook is written by experts and professionals in membrane applications from around the world. Key Features: • Includes entirely new chapters on organic solvent-resistant nanofiltration, membrane condensers, membrane-reactors in hydrogen production, membrane materials for haemodialysis, and integrated membrane distillation • Covers the full spectrum of membrane technology and its advancements • Explores membrane applications in a range of fields, from biotechnological and food processing to industrial waste management and environmental engineering This book will appeal to both newcomers to membrane science as well as engineers and scientists looking to expand their knowledge on upcoming advancements in the field.
Download or read book Nanofiltration Membranes written by Lau Woei Jye and published by CRC Press. This book was released on 2016-12-19 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering fabrication, characterization, and applications nanofiltration (NF) membranes, this book provides a comprehensive overview of the development of NF membrane technology over the past decade. It uniquely covers a variety of fabrication techniques, comparing the procedures of each technique to produce polymeric membranes of different morphologies. The book also discusses advances in the materials used in thin film composite (TFC) polyamide membrane fabrication and their influences on properties with respect to structural and separation characteristics. A comprehensive review on NF characterization methods and techniques is provided, assessing physical and chemical properties and separation characteristics and stability. Technical challenges in fabricating a new generation of NF membranes are also reviewed and the possible approaches to overcome the challenges are provided. The book concludes with relevant case studies on the use of NF membranes in industrial implementation of both aqueous and nonaqueous media. Details the latest progress on the fabrication techniques of asymmetric and composite NF membranes. Discusses characterization methods used in assessing membrane physical/chemical properties, separation characteristics, and performance stability. Describes the potential of advanced materials in improving properties of polyamide selective layer as well as microporous substrate. Reviews the technical challenges in fabricating a new generation of composite membrane—thin film nanocomposite (TFN) membrane—possible approaches to overcome challenges. Offers case studies on the applications of NF membranes for both aqueous and nonaqueous media.
Download or read book Nanoparticle Impact on Membrane Properties and Embedded Ion Channels written by Isabel Ursula Foreman-Ortiz and published by . This book was released on 2021 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Design of nanoscale materials for applications that interface with biological systems requires that mechanisms of nanoparticle interaction with cellular membranes be understood, both to improve performance and to assess nanomaterial safety. Model lipid bilayer systems are often used in studying nano-bio interactions because they simplify living systems to focus on individual membrane components, revealing mechanistic insight that would otherwise be obscured by the functional complexity of the cell. Here, we describe a series of studies that investigated anionic nanoparticle interaction with phospholipid membranes containing an embedded model ion channel (gramicidin A). Ion channels comprise a class of embedded membrane proteins that are critical for maintaining cellular homeostasis and other biological functions (e.g., epithelial transport, immune cell activation, conduction of nerve impulses, muscle contraction). Employing this model membrane system allowed us to study the impact of anionic gold nanoparticles on the function of gramicidin A ion channels, assess the effect of the presence of gramicidin A on nanoparticle binding, and reveal a mechanism by which AuNPs can exert effects on embedded ion channels. Our results indicate that nanoparticle effects on membrane protein function do not require direct interaction and can instead be mediated via changes in bilayer mechanical properties. Moreover, we present a novel combination of single-particle tracking and statistical mixture analysis to assess the impact of the gramicidin A ion channel on the extent of anionic gold nanoparticle binding.
Download or read book Biomimetic Nanotechnology written by Anja Mueller and published by Walter de Gruyter GmbH & Co KG. This book was released on 2023-04-03 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an interdisciplinary book for biomimetic nanotechnology, that correlates the biology on the molecular scale with nanotechnology mimicking human senses and movement. The introduction provides the background in life science, chemistry, material science, and engineering needed to understand sensors and movement on the molecular level. The chapters discuss human movement, vision, smell and taste, hearing, and touch. Each chapter explains the sense or movement on the molecular level, then discusses nanotechnology that uses the human molecules or mimics the function of the human sense and movement on the nanoscale. This is an excellent book for senior undergraduates and graduate students in the life sciences, chemistry, material sciences, and engineering. It will also appeal to any reader with an interest in life sciences and nanotechnology.
Download or read book Supported Lipid Membranes and Their Use for the Characterization of Biological Nanoparticles written by Silver Jõemetsa and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Membrane Separations written by Anil K. Pabby and published by CRC Press. This book was released on 2015-04-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Membrane Separations: Chemical, Pharmaceutical, Food, and Biotechnological Applications, Second Edition provides detailed information on membrane separation technologies from an international team of experts. The handbook fills an important gap in the current literature by providing a comprehensive discussion of membrane applications in the chemical, food, pharmaceutical, and biotechnology industries as well as in the treatment of toxic industrial effluents. This revised second edition has been updated and expanded with discussions of new membrane products and processes and novel applications in engineering, life sciences, and energy conversion. It also includes new chapters in the field of membrane science and technology covering recent advances in RO and UF, ionic liquids, nanotechnology, roles of membrane in power generation, updates on fuel cells, new membrane extraction configuration, and other important topics. The handbook is equally suited for the newcomer to the field as it is for process engineers and research scientists (membranologists/membrane experts) who are interested in obtaining more advanced information about specific applications. It provides readers with a comprehensive and well-balanced overview of the present state of membrane science and technology.
Download or read book Interaction of Amphphilic Nanoparticles with Model and Bacterial Membranes written by Jared Thomas Wiemann and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this doctoral work, we aimed to understand how amphiphilic nanoparticles, especially those with anisotropic surface chemistry, interact with biological membranes. Specifically, our overall goal was to elucidate the cytotoxic and antibacterial properties of anisotropic particles relative to amphiphilic nanoparticles with uniform surface chemistry. Nanoparticles with anisotropic surface chemistry have been synthesized for uses in sensing and immunotherapy, among other uses. In our early studies, we evaluated the impact of amphiphilic particles against free-standing biomimetic membranes to assess their potential cytotoxicity. Using “two-faced” amphiphilic Janus nanoparticles, which display separated hydrophobic and hydrophilic sides, we observed that anisotropic surface chemistry enhances the binding of particles to lipid membranes which leads to poration, membrane wrinkling, protrusions, and even collapse of entire membranes. Building upon these findings, we further observed that Janus nanoparticles selectively bind to liquid disordered domains in phase-separated lipid membranes. Extraction of lipids from liquid disordered domains to the hydrophobic nanoparticle hemispheres led to membrane compression and bulging of liquid ordered domains. These studies revealed the distinct consequences of anisotropic amphiphilic nanoparticles on lipid membrane integrity and structure. In the final part of this work, we investigated the antimicrobial properties of amphiphilic Janus nanoparticles. As more multidrug resistant bacteria emerge and fewer antibiotics are developed for clinical use, antibiotic resistance remains an increasing threat to public health. Researchers have since turned to nanomaterials with tailored surface chemistry to evade mechanisms of antibiotic resistance by directly disrupting lipid membranes and cell walls, often times using uniform coatings of cationic or amphiphilic ligands on particle surfaces. Given the potential enhanced antibiotic properties of Janus nanoparticles based on our first works, we fabricated “two-faced” amphiphilic Janus nanoparticles with one hemisphere of the Janus nanoparticles functionalized with hydrophobic alkyl chains and the other hemisphere of the nanoparticles functionalized with either a cationic antibiotic drug, colistin, or a cationic poly(amidoamine) (PAMAM) dendrimer that is not an antibiotic drug. We show that both the colistin/hydrophobic Janus nanoparticles and dendrimer/hydrophobic Janus nanoparticles effectively inhibit the growth of both Gram-negative and Gram-positive bacteria at picomolar concentrations. The half-maximum effective concentrations (EC50) of those cationic/hydrophobic Janus nanoparticles against a variety of bacteria are in the picomolar range and significantly lower than those of conventional nanoparticles with uniform surface chemistry. The Janus nanoparticles inhibit bacterial growth by disrupting bacterial membranes and by inducing reactive oxygen species (ROS). Our results demonstrate that the design of the segregated hydrophobic and cationic charges on the nanoparticle surface is key for their antibiotic potency. The antibiotic potency of the Janus nanoparticles is applicable to cationic ligands and not limited to cationic antibiotic molecules. Those anisotropic amphiphilic Janus nanoparticles are promising new broad-spectrum antibiotic nanoparticles.
Download or read book Nanoscale Fabrication Optimization Scale up and Biological Aspects of Pharmaceutical Nanotechnology written by Alexandru Mihai Grumezescu and published by William Andrew. This book was released on 2017-12-11 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoscale Fabrication, Optimization, Scale-up and Biological Aspects of Pharmaceutical Nanotechnology focuses on the fabrication, optimization, scale-up and biological aspects of pharmaceutical nanotechnology. In particular, the following aspects of nanoparticle preparation methods are discussed: the need for less toxic reagents, simplification of the procedure to allow economic scale-up, and optimization to improve yield and entrapment efficiency. Written by a diverse range of international researchers, the chapters examine characterization and manufacturing of nanomaterials for pharmaceutical applications. Regulatory and policy aspects are also discussed. This book is a valuable reference resource for researchers in both academia and the pharmaceutical industry who want to learn more about how nanomaterials can best be utilized. - Shows how nanomanufacturing techniques can help to create more effective, cheaper pharmaceutical products - Explores how nanofabrication techniques developed in the lab have been translated to commercial applications in recent years - Explains safety and regulatory aspects of the use of nanomanufacturing processes in the pharmaceutical industry
Download or read book Ion Exchange Chromatography and Related Techniques written by Pavel Nesterenko and published by Elsevier. This book was released on 2023-11-15 with total page 646 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ion-Exchange Chromatography and Related Techniques defines the current state-of-the-art in ion-exchange chromatography and related techniques and their implementation in laboratory and industrial practice. This book provides a compact source of information to facilitate the transfer of knowledge and experience acquired by separation science specialists to colleagues from diverse backgrounds who need to acquire fundamental and practical information to facilitate progress in research and management functions reliant on information acquired by separation. Individual chapters written by recognized experts lending credibility to the work will allow this book to serve as a high value reference source of current information for analytical and biopharmaceutical chemists. - Includes individual chapters written by recognized authoritative and visionary experts in the field to provide an overview and focused treatment of a single topic - Presents comprehensive coverage of ion-exchange techniques from theory, to methods, to selected applications for ions and biopolymers - Provides Tables and diagrams with commonly used data to facilitate practical work, comparison of results and decision-making
Download or read book Single Chain Polymer Nanoparticles written by José A. Pomposo and published by John Wiley & Sons. This book was released on 2017-08-18 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first book on this important and emerging topic presents an overview of the very latest results obtained in single-chain polymer nanoparticles obtained by folding synthetic single polymer chains, painting a complete picture from synthesis via characterization to everyday applications. The initial chapters describe the synthetics methods as well as the molecular simulation of these nanoparticles, while subsequent chapters discuss the analytical techniques that are applied to characterize them, including size and structural characterization as well as scattering techniques. The final chapters are then devoted to the practical applications in nanomedicine, sensing, catalysis and several other uses, concluding with a look at the future for such nanoparticles. Essential reading for polymer and materials scientists, materials engineers, biochemists as well as environmental chemists.
Download or read book Effects of the Nanoparticle Protein Corona on Nanoparticle cell Membrane Interaction written by Mohamad Javad Haghighat Manesh and published by . This book was released on 2020 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increasing applications of nanoparticles in biomedical research and consumer products has raised concerns regarding their potential toxicity. Although the effects of nanoparticles on human health and environment are still under investigation, previous studies have revealed significant evidence of nanoparticle toxicity to cells. With the cell plasma membrane being the first entity that first interacts with nanoparticles upon possible exposure, numerous studies have focused on nanoparticle interactions with cell plasma membrane. Nanoparticles entering a biological fluid are covered by proteins which adsorb to the particle surface, commonly called the nanoparticle protein corona. Protein corona changes nanoparticle characteristics, thereby altering their biological behavior. A better understanding of how the corona regulates nanoparticle-membrane interactions can help the design of the biomedical applications of nanoparticles. This study seeks to investigate the role of the corona from human serum on nanoparticle-induced cell membrane damage. Plain silica nanoparticles, known for disrupting the lipid bilayer of the cell membrane, were used as nanoparticle models. The effects of pristine nanoparticles are recorded after interactions with model membranes and red blood cells and compared to results from nanoparticles with the protein corona on their surface.
