Download or read book Melanoma written by Kristian M. Hargadon and published by Humana. This book was released on 2022-03-26 with total page 674 pages. Available in PDF, EPUB and Kindle. Book excerpt: This extensive book brings together leading melanoma researchers from across the world and highlights many of the cutting-edge protocols and experimental systems currently being used to investigate questions surrounding this disease. The volume opens with sections on 2D and 3D cell culture-based approaches for studying melanoma biology, and continues with collections of chapters examining various approaches for detecting, isolating, and characterizing circulating melanoma cells, circulating tumor DNA, and exosomes, as well as experimental procedures for studying and detecting melanoma metastasis in both pre-clinical and clinical settings, bioinformatics-based approaches, protocols for quantifying and characterizing immune cell infiltrates in both melanoma tumors and tertiary lymphoid structures, and development and evaluation of therapeutic strategies for melanoma treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Melanoma: Methods and Protocols aims to serve basic research scientists and clinicians who bring questions from the clinic into the lab in order to translate observations in the laboratory into improved patient care for this highly malignant form of cancer. Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Melanoma Techniques and Protocols written by Brian J. Nickoloff and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is about melanoma—its biology, immunology, and pathology, as well as the initial use of powerful genomic tools to study its fundamental mole- lar and genetic characteristics. The study of cancer will be profoundly impacted by the Human Genome Project. I would like to discuss some of these changes. The first draft of the human genome sequence was announced in June 2000, and we have just scratched the surface of the changes it will engender in medicine. A relevant question is what are the long-term effects of the Human Genome Project for medicine? I would argue that there are three, and each of these three point toward the view that systems biology will dominate biology and medicine of the 21st century. First, the Human Genome Project introduced a new type of s- ence—discovery science. Discovery science takes a biological system (e. g. , the genome) and defines all of its elements (e. g. , the sequences of the 24 human ch- mosomes). Thus, it creates a rich infrastructure from which the classical hypo- esis-driven science can be done more effectively. The effective integration of discovery- and hypothesis-driven science is a key for systems approaches to bi- ogy and medicine. Second, the Human Genome Project has provided a “periodic table of life.
Download or read book Melanoma written by Kristian M. Hargadon and published by . This book was released on 2021 with total page 674 pages. Available in PDF, EPUB and Kindle. Book excerpt: This extensive book brings together leading melanoma researchers from across the world and highlights many of the cutting-edge protocols and experimental systems currently being used to investigate questions surrounding this disease. The volume opens with sections on 2D and 3D cell culture-based approaches for studying melanoma biology, and continues with collections of chapters examining various approaches for detecting, isolating, and characterizing circulating melanoma cells, circulating tumor DNA, and exosomes, as well as experimental procedures for studying and detecting melanoma metastasis in both pre-clinical and clinical settings, bioinformatics-based approaches, protocols for quantifying and characterizing immune cell infiltrates in both melanoma tumors and tertiary lymphoid structures, and development and evaluation of therapeutic strategies for melanoma treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Melanoma: Methods and Protocols aims to serve basic research scientists and clinicians who bring questions from the clinic into the lab in order to translate observations in the laboratory into improved patient care for this highly malignant form of cancer. Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Molecular Diagnostics for Melanoma written by Magdalena Thurin and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Molecular Diagnostics for Melanoma: Methods and Protocols, expert researchers and clinicians in the field of melanoma provide updated information on biomarkers and assays for diagnosis, prognosis, and assays predicting response to treatment for routine testing. The focus of the volume is on biomarkers with established clinical validity rather than those on early discovery stage. With additional in-depth discussion of the molecular biology and pathology of melanoma, treatment options in adjuvant and metastatic setting, and implications of biomarker testing for clinical management of melanoma patients. Written in the highly successful Methods in Molecular Biology series format, chapters include extensive introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Molecular Diagnostics for Melanoma: Methods and Protocols seeks to provide both clinicians and scientists with technical information and extensive background information on the wide ranging approaches available in the field of diagnostics of melanoma.
Download or read book Cancer Cell Culture written by Simon P. Langdon and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.
Download or read book Cutaneous Melanoma written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Non Melanoma Skin Cancer and Cutaneous Melanoma written by Othon Papadopoulos and published by Springer Nature. This book was released on 2020-06-17 with total page 844 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes and illustrates the reconstructive surgical techniques appropriate in patients with skin cancer and melanoma, according to anatomic region, with a view to enabling readers to perform these techniques optimally. An extensive introductory section discusses skin cancer and melanoma from the points of view of dermatology, oncology, pathology, radiotherapy, nuclear medicine, and plastic and reconstructive surgery. The surgical treatment and reconstruction of localized skin cancer and melanoma in each anatomic region are then explained step by step with the aid of a wealth of color photos. The final section similarly presents the techniques and procedures appropriate for the surgical treatment and reconstruction of lymph node metastases, covering the sentinel node, lymphadenectomy, and lymphedema. This book will be immensely valuable for plastic and general surgeons, and also of interest for dermatologists, pathologists, oncologists, and nuclear medicine physicians.
Download or read book Practical Manual for Dermatologic and Surgical Melanoma Management written by Delphine J. Lee and published by Springer Nature. This book was released on 2020-05-11 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a practically applicable resource for all clinicians managing patients with or who may develop melanoma. Each chapter focuses on clinically relevant information on the latest advances in the field, including techniques for early detection of skin cancers, cross-sectional imaging and staging of regional nodes. Algorithms for clinical decision-making along with clinical vignettes are incorporated into each chapter, enabling the reader to develop a deep understanding of how to manage a range of scenarios. Practical Manual for Dermatologic and Surgical Melanoma Management systematically details the latest diagnostic criteria, treatment guidelines and management techniques available for treating these patients and is a valuable resource for the trainee and practising clinician.
Download or read book Biomarkers for Immunotherapy of Cancer written by Magdalena Thurin and published by Humana. This book was released on 2019-09-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the immune oncology (IO) community with a deeper understanding of the scope of the biomarker methods to potentially improve the outcome from immunotherapy. The editors secured the input from experts in the field dedicated to translating scientific research from bench to bedside was submitted. The book provides not only details about the technical, standardization and interpretation aspects of the methods but also introduces the reader to the background information and scientific justification for selected biomarkers and assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Cutaneous Melanoma written by Charles M. Balch and published by Springer. This book was released on 2020-01-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the leading melanoma experts from the United States, Australia, and Europe, this new edition incorporates the clinical outcomes of more than 70,000 patients treated at major melanoma centers throughout the world and is the definitive and most authoritative textbook on melanoma used worldwide. Providing the most up-to-date and comprehensive information needed for the clinical management and scientific study of melanoma, Cutaneous Melanoma, 6th edition covers everything from precursors of melanoma to advanced stages of metastatic disease.
Download or read book Cancer Cell Culture written by Ian A. Cree and published by Humana Press. This book was released on 2016-08-23 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.
Download or read book Cancer Immunoprevention written by Florencia McAllister and published by . This book was released on 2022 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides methods and techniques to further the study of cancer immunoprevention. Chapters describe tumor-associated antigens, cancer immune-preventive vaccines, generation of TILs, development of monoclonal antibodies, immunoprofiling technologies, tissue multispectral imaging techniques, mass cytometry on suspensions, mutiparametric flow cytometry, genomic expression analysis, and proteomic profiling of tumor microenvironment cell populations and metabolic assessment through novel imaging technologies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Cancer Immunoprevention: Methods and Protocol aims to further understanding, development of interventional active strategies, and immune-interception of cancer.
Download or read book Melanoma written by Howard L. Kaufman and published by Springer. This book was released on 2015-11-24 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Melanoma is one of the most types of cancer. When melanoma is detected at an early stage, treatment is highly successful, but outcomes can be poor when the disease is advanced. There has been significant progress in our understanding of the molecular biology, genetics, and immunology of melanoma over the past decade. This has been accompanied by rapid advances in therapeutic strategies for patients with melanoma. This book provides the clinician and the researcher with a broad understanding of the molecular and cellular pathogenesis of melanoma, explores the clinical characteristics and criteria for clinical and pathological staging of the disease, and provides an overview of current and evolving treatment strategies in the adjuvant, metastatic, and preventive settings. The treatment of special populations and rare variants of melanoma that often present particular clinical challenges is also covered. Authored by international experts in melanoma biology and clinical management, this volume concisely explains how to diagnose, treat, and prevent melanoma while reviewing advances in basic science and providing an overview of innovative approaches still under development.
Download or read book Surgical Treatment written by René Holzheimer and published by . This book was released on 2001 with total page 880 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book General Surgery written by Kirby I. Bland and published by Springer Science & Business Media. This book was released on 2008-11-12 with total page 2068 pages. Available in PDF, EPUB and Kindle. Book excerpt: General Surgery: Principles and International Practice is organized over two volumes into ten Sections, each representing an important branch of surgical science. Amply supported by line drawings and photographs, algorithms and anatomical depictions, it provides illustrative, instructive and comprehensive coverage depicting the rationale for the basic operative principles mandated by state-of-the-art surgical therapy.
Download or read book Sentinel Lymph Node Biopsy written by Hiram S. Cody and published by CRC Press. This book was released on 2001-11-08 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: An intuitive, ingenious and powerful technique, sentinel lymph node biopsy has entered clinical practice with astonishing rapidity and now represents a new standard of care for melanoma and breast cancer patients, while showing great promise for the treatment of urologic, colorectal, gynecologic, and head and neck cancers. This text, written by international experts in the technique, provides a clear and comprehensive guide, presenting a detailed overview and discussing the various mapping techniques available and how these are applied in a number of leading institutions. This essential resource for surgical onocologists, pathologists, and specialists in nuclear medicine will also provide key information for those planning to start a sentinel lymph node program.
Download or read book Metastasis Research Protocols written by Susan A. Brooks and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Volume I, Analysis of Cells and Tissues, we presented a range of protocols aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research protocols, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systems in turn must of course be put into perspective relative to their roles in entire living organisms.
