Download or read book Mechano biological Regulation of Human Mesenchymal Stem Cell Osteogenic Differentiation written by Nelson John Tacy and published by . This book was released on 2006 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mechanical and Biological Mechanisms of Regulating Human Mesenchymal Stem Cell Differentiation written by Anne Kathryn Staples and published by . This book was released on 2006 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of how hMSCs respond to different types of mechanical loading, how this response differs from a traditional growth factor approach of inducing cellular differentiation and how their responsiveness to mechanical stimulation varies with cell differentiation stage are all critical for the successful design of tissue engineering constructs that are optimally organized for a specific mechanical function.

Download or read book Essentials of Mesenchymal Stem Cell Biology and Its Clinical Translation written by Robert Chunhua Zhao and published by Springer Science & Business Media. This book was released on 2013-06-05 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesenchymal stem cells (MSCs), a type of adult stem cells, have attracted the attention of scientists and physicians alike due to their unique biological properties and potential for disease treatment. As stem cell research is complex and progressing rapidly, it is important that the experts in this field share their views and perspectives. This book, co-edited by leading global researchers, is divided into three major sections and covers a broad range of topics concerning MSCs during their transition from benchside to bedside. The book is intended for researchers and clinicians in the field of stem cells. Dr. Robert Chunhua Zhao, MD. Ph.D is Cheung Kong Professor of Stem Cell Biology, Professor of Cell Biology at the Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, China Director of Center for Tissue Engineering, PUMC Chief scientist of 973 program Regional Editor of Stem Cells and Development.

Download or read book The Biology and Therapeutic Application of Mesenchymal Cells written by Kerry Atkinson and published by John Wiley & Sons. This book was released on 2016-11-08 with total page 1007 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Biology and Therapeutic Application of Mesenchymal Cells comprehensively describes the cellular and molecular biology of mesenchymal stem cells and mesenchymal stromal cells, describing their therapeutic potential in a wide variety of preclinical models of human diseases and their mechanism of action in these preclinical models. Chapters also discuss the current status of the use of mesenchymal stem and stromal cells in clinical trials in a wide range of human diseases and disorders, for many of which there are limited, or no other, therapeutic avenues. Provides coverage on both the biology of mesenchymal stem cells and stromal cells, and their therapeutic applications Describes the therapeutic potential of mesenchymal stem and stromal cells in a wide variety of preclinical models of human diseases and their mechanism of action in these preclinical models Discusses the current status of mesenchymal stem and stromal cells in clinical trials in a wide range of human diseases and disorders, for many of which there are limited, or no other, therapeutic avenues Written and edited by leaders in the field The Biology and Therapeutic Application of Mesenchymal Cells is an invaluable resource for those studying stem cells, cell biology, genetics, gene or cell therapy, or regenerative medicine.

Download or read book Mesenchymal Stem Cells Basics and Clinical Application I written by Birgit Weyand and published by Springer. This book was released on 2014-07-08 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prospective Isolation and Characterization of Human Bone Marrow-Derived MSCs, by A. Harichandan, K. Sivasubramaniyan, H.-J. Bühring Urine as a Source of Stem Cells, by Christina Benda, Ting Zhou, Xianming Wang, Weihua Tian, Johannes Grillari, Hung-Fat Tse, Regina Grillari-Voglauer, Duanqing Pei, Miguel A. Esteban Expansion of Mesenchymal Stem/Stromal Cells under Xenogenic-Free Culture Conditions, by Sven Kinzebach, Karen Bieback Adipose-Derived Mesenchymal Stem Cells: Biology and Potential Applications, by Danielle Minteer, Kacey G Marra, J Peter Rubin Potential for Osteogenic and Chondrogenic Differentiation of MSC, by Antonina Lavrentieva, Tim Hatlapatka, Anne Neumann, Birgit Weyand, Cornelia Kasper Potential for Neural Differentiation of Mesenchymal Stem Cells, by Letizia Ferroni, Chiara Gardin, Ilaria Tocco, Roberta Epis, Alessandro Casadei, Vincenzo Vindigni, Giuseppe Mucci, Barbara Zavan Migratory Properties of Mesenchymal Stem Cells, by Thomas Dittmar, Frank Entschladen Dissecting Paracrine Effectors for Mesenchymal Stem Cells, by Stefania Bruno, Federica Collino, Ciro Tetta, Giovanni Camussi Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells, by Yin Xiao, Jiezhong Chen Does the Adult Stroma Contain Stem Cells?, by Richard Schäfer

Download or read book Biomaterials as Stem Cell Niche written by Krishnendu Roy and published by Springer Science & Business Media. This book was released on 2010-09-30 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in stem cell biology have opened new directions in cell therapy. This book provides the state-of-the-art developments in using biomaterials as artificial niches for engineering stem cells, both for the purpose of better understanding their biology under 3D biomimetic conditions as well as for developing new strategies for efficient long term maintenance and directed differentiation of stem cells into various therapeutic lineages. Animal and human stem cells of both embryonic and adult origin are discussed with applications ranging from nerve regeneration, orthopedics, cardiovascular therapy, blood cell generation and cancer therapy. Both synthetic and natural biomaterials are reviewed with emphasis on how material-stem cell interactions direct specific signaling pathways and ultimately modulate the cell fate. This book is valuable for biomaterial scientists, tissue engineers, clinicians as well as stem cell biologists involved in basic research and applications of adult and embryonic stem cells.

Download or read book Mesenchymal Stem Cell Therapy written by Lucas G. Chase and published by Springer Science & Business Media. This book was released on 2012-12-12 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past decade, significant efforts have been made to develop stem cell-based therapies for difficult to treat diseases. Multipotent mesenchymal stromal cells, also referred to as mesenchymal stem cells (MSCs), appear to hold great promise in regards to a regenerative cell-based therapy for the treatment of these diseases. Currently, more than 200 clinical trials are underway worldwide exploring the use of MSCs for the treatment of a wide range of disorders including bone, cartilage and tendon damage, myocardial infarction, graft-versus-host disease, Crohn’s disease, diabetes, multiple sclerosis, critical limb ischemia and many others. MSCs were first identified by Friendenstein and colleagues as an adherent stromal cell population within the bone marrow with the ability to form clonogenic colonies in vitro. In regards to the basic biology associated with MSCs, there has been tremendous progress towards understanding this cell population’s phenotype and function from a range of tissue sources. Despite enormous progress and an overall increased understanding of MSCs at the molecular and cellular level, several critical questions remain to be answered in regards to the use of these cells in therapeutic applications. Clinically, both autologous and allogenic approaches for the transplantation of MSCs are being explored. Several of the processing steps needed for the clinical application of MSCs, including isolation from various tissues, scalable in vitro expansion, cell banking, dose preparation, quality control parameters, delivery methods and numerous others are being extensively studied. Despite a significant number of ongoing clinical trials, none of the current therapeutic approaches have, at this point, become a standard of care treatment. Although exceptionally promising, the clinical translation of MSC-based therapies is still a work in progress. The extensive number of ongoing clinical trials is expected to provide a clearer path forward for the realization and implementation of MSCs in regenerative medicine. Towards this end, reviews of current clinical trial results and discussions of relevant topics association with the clinical application of MSCs are compiled in this book from some of the leading researchers in this exciting and rapidly advancing field. Although not absolutely all-inclusive, we hope the chapters within this book can promote and enable a better understanding of the translation of MSCs from bench-to-bedside and inspire researchers to further explore this promising and quickly evolving field.

Download or read book Mechanobiologic Regulation of Skeletal Progenitor Cell Differentiation written by Juan Manuel Taboas and published by . This book was released on 2004 with total page 844 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cyclic Tensile Strain Promotes COL I Induced Osteogenic Differentiation and Enhances Lineage Commitment Gene Focusing of Human Mesenchymal Stem Cells written by Donald F. Ward (Jr) and published by ProQuest. This book was released on 2007 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Multi parameter Assessment of Mechano sensitivity Driven Differentiation of Human Mesenchymal Stem Cells written by Lara Hauke and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: What factors and conditions control stem cell differentiation is currently one of the major questions in biomedical research. What factors ultimately influence the cells' decision is unclear but it has been shown, that stem cells differentiate reversibly through mechanical clues alone, expressing specific tissue markers as early as several days after plating on a substrate with similar stiffness. Stem cells cultured on elastic substrates with different Young's moduli E exhibit distinct structure formation of their cytoskeletal organization already within the first 24 hours. Whether this is ...

Download or read book Modulation of Osteogenic Differentiation in Human Mesenchymal Stem Cells by Submicron Topographically patterned Ridges and Grooves written by Shinya Watari and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent studies have shown that nanoscale and submicron topographic cues modulate a menu of fundamental cell behaviors, and the use of topographic cues is an emerging area of tissue engineering. We used topographically-patterned substrates containing anisotropically-ordered ridges and grooves to mimic one feature of the biophysical environment of type I collagen, which consists of aligned nano- to submicron-scale fibrils. We investigated the effects of topographic cues on mesenchymal stem cell morphology, proliferation, and osteogenic differentiation. Cells cultured on 1400 or 4000 nm pitches showed greater elongation and alignment relative to 400 nm pitch or planar control. Cells cultured on 400 nm pitch demonstrated significant increases in RUNX2 and BGLAP expression relative to cells cultured on 1400 or 4000 nm pitch or planar control. 400 nm pitch enhanced extracellular calcium deposition. Cells cultured in osteoinductive medium revealed combinatory effects of topography and chemical cues on 400 nm pitch as well as up-regulation of expression of ID1, a target of the BMP pathway. Our data demonstrate that a specific size scale of topographic cue promotes osteogenic differentiation with or without osteogenic agents. These data demonstrate that the integration of topographic cues may be useful for the fabrication of orthopedic implants.

Download or read book Thermal Regulation of Human Mesenchymal Stem Cell Differentiation Toward Bone and Cartilage Lineages written by Jing Chen and published by . This book was released on 2013 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book BMI1 Regulates Self renewal and Differentiation of Human Mesenchymal Stem Cells written by Yun Joon Jung and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the central questions in stem cell biology is to understand how stem cells regulate self-renewal and differentiation. Recently, polycomb group (PcG) proteins have been associated with the regulation of adult stem cells. BMI1, a constituent of PcG proteins, is known to be required for self-renewal in many tissues but its role in mesenchymal stem cells (MSCs) remains to be illustrated. Here, loss-of-function and gain-of-functions analysis in MSCs point out that BMI1 is critical for the MSC self-renewal and cell fate decision. Knockdown of BMI1 prevents cell proliferation induced by p14ARF/p16INK4A and increases apoptosis. Gain of functions analysis reveals that overexpression of BMI1 reduces apoptosis and increases cell proliferation by repressing p14ARF/p16INK4A. In addition, BMI1 is crucial for regulation of the multipotency of MSCs. Loss of BMI1 resulted in reduction in MSC differentiation potential due to cell cycle inhibition. Overexpression of BMI1 caused repression of transcripts of differentiation-related genes, RUNX2 and PPAR[gamma], in osteogenesis and adipogenesis, respectively. Thus, reduced expression of these genes leads to decreased differentiation potential in MSCs. My data, therefore, suggests that BMI1 regulates the self-renewal of MSCs.

Download or read book Laminin 5 Promotes Intracellular Signaling Pathways Resulting in the Osteogenic Differentiation of Human Mesenchymal Stem Cells written by Robert F. Klees (Jr) and published by . This book was released on 2007 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Computational and Experimental Analyses of Bone and Adult Stem Cell Mechanobiology written by and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Human bone marrow and adipose derived adult stem cells have shown great promise as a source of expandable and differentiable cells for tissue engineering and regenerative medicine. However, a more complete understanding of the optimal in vitro culture conditions is required to create functional engineered tissue. Especially key in the context of musculoskeletal tissue is the in vitro generation of nascent tissue with appropriate material properties for withstanding in vivo loading are the combined mechanical and chemical stimuli used to culture adult stem cells. Previous studies have shown that mechanical loading induces differentiation of human mesenchymal stem cells (hMSCs) and mesenchymal tissue into tissues such as bone, fibrous tissue, cartilage, and smooth muscle cells. More recently, human adipose derived adult stem cells (hASCs) have shown promise for similar tissue engineering applications. Fluid shear stress is believed to be one of the primary stimuli for osteocytes in the maintenance of mature bone, and tensile strain has been shown to induce the formation and repair of bone from mesenchymal tissue. However, the proper range of local stresses and strains required for stem cell-induced bone formation in vitro have yet to be determined. This body of work examined the application of different methods of external loading on human adult stem cells to induce their osteogenic differentiation. Both experimental and computational analyses were completed in order to provide a detailed understanding of cellular response to loading, and the local stresses and strains placed on cells during loading. Fluid shear stresses were applied to hASCs on and within a porous 3D scaffold to measure upregulation of osteogenic genetic markers. Cyclic tensile strain was applied to hASCs in 2D culture and calcium deposition was quantified as a measure of mRNA expression of osteogenic differentiation. Computational models were created to determine the range and location of different.

Download or read book Methods of Tissue Engineering written by Anthony Atala and published by Gulf Professional Publishing. This book was released on 2001-10-12 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book combines the tools, experimental protocols, detailed descriptions and know-how for the successful engineering of tissues and organs in one volume.

Download or read book The TGF beta Family written by Rik Derynck and published by CSHL Press. This book was released on 2008 with total page 1108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transforming growth factor-[beta] (TGF-[beta]), identified nearly three decades ago, is a secreted polypeptide that functions in critical cell cycle processes, including cellular proliferation, differentiation, and development: It belongs to a large protein family that, in humans, contains 33 members, including activins, inhibins, bone morphogenetic proteins, growth and differentiation factors, and Mullerian inhibiting substance. This volume draws on the world's leading laboratories to comprehensively cover all aspects of the biology of TGF-[beta] and related factors. In addition to providing historical and background information, it describes the cell biology and signaling pathways of TGF-[beta] members in detail, including the roles of TGF-[beta] factors in the development and physiology of humans and model organisms. The last few chapters are devoted to the role of TGF-[beta] members in cancer and other diseases, as well as the possibilities for therapeutics based on knowledge of signaling pathways and macromolecular structures. It serves as a comprehensive reference work for both specialists and researchers less familiar with the field.