Download or read book Mechanisms of Resistance in Carbofuran resistant Colorado Potato Beetles written by Joel Martin Wierenga and published by . This book was released on 1992 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization Synergism and Inheritance of Resistance to Azinphosmethyl Carbofurna and Permethrin Insecticides in the Colorado Potato Beetle Coleoptera Chrysomelidae written by Philippos M. Ioannidis and published by . This book was released on 1990 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Resistance and Metabolism of Imidacloprid in Colorado Potato Beetle Leptinotarsa Decemlineata Say Coleoptera Chrysomelidae written by David Mota-Sanchez and published by . This book was released on 2002 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Detection of Mutations in Colorado Potato Beetle written by Jessica Bridget Dunn and published by . This book was released on 2000 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanism of Resistance Against Insecticidal Double Stranded RNA dsRNA in Leptinotarsa Decemlineata Colorado Potato Beetle written by Swati Mishra (Researcher in Entomology) and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, silencing of vital genes by RNA interference (RNAi) through dsRNA ingestion has been identified as a revolutionary bioinsecticide technology. As commercialization of insecticidal dsRNA technology approaches, it becomes crucial to develop resistance management tools for the sustainability of this technology. Using chronic exposure through larval development, we developed a population (CEAS) of Colorado potato beetle (CPB) that is >5,000-fold resistant to insecticidal dsRNA. In this work we share findings from research focused on the identification of candidate resistance mechanisms and cross-resistance to Cry3Aa, the most active insecticidal protein from Bacillus thuringiensis against CPB. Bioassays and comparison of dsRNA stability in digestive fluids from susceptible and resistant CPB support cross-resistance to alternative dsRNA targets and that degradation of dsRNA by nucleases is not involved in resistance. Monitoring uptake of fluorescently-labeled dsRNA by midgut cells using confocal microscopy supports reduced uptake of dsRNA in midgut cells of CEAS compared to susceptible larvae. Results from this project will guide development of Insect Resistance Management (IRM) strategies for insecticidal RNAi and its combined used with insecticidal proteins from B. thuringiensis against CPB and will allow the optimization of insecticidal RNAi technology.
Download or read book Studies on Mechanisms of Resistance in the Wild Potato Solanum Berthaultii Hawkes to the Colorado Potato Beetle Leptinotarsa Decemlineata Say Coleoptera Chrysomelidae written by Michael Brent Dimock and published by . This book was released on 1985 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Combining Engineered Bt cry3A and Natural Resistance Mechanisms in Potato Solanum Tuberosum L for Control of the Colorado Potato Beetle Leptinotarsa Decemlineata Say written by Joseph John Coombs and published by . This book was released on 2000 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Managing Insecticide Resistance in the Colorado Potato Beetle Leptinotarsa Decemlineata Say written by Steven P. Mroczkiewicz and published by . This book was released on 1993 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Novel Mechanism for Resistance to Colorado Potato Beetle Leptinotarsa Decemlineata in Wild Solanum Species written by Evans Olonyi Sikinyi and published by . This book was released on 1996 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Insecticide Resistance in Colorado Potato Beetles written by Andrei Alyokhin and published by . This book was released on 2008 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization Synergism and Inheritance of Resistance to Azinphosmethyl Carbofuran and Permethrin Insecticides in the Colorado Potato Beetle Coleoptera Chrysomelidae written by Philippos M. Ioannidis and published by . This book was released on 1990 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Bacillus Thuringiensis Resistant Strain of Colorado Potato Beetle Coleoptera chrysomedlidae written by Y. Andi Trisyono and published by . This book was released on 1994 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Natural Plant Based Resistance Substances Against the Colorado Potato Beetle and Their Possible Mechanism of Action written by K. Schreiber and published by . This book was released on 1957 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Growth and Development of Insecticide resistant and Susceptible Colorado Potato Beetle Larvae Coleoptera Chrysomelidae on Different Solanaceous Hosts written by Patti Lea Rattlingourd and published by . This book was released on 1991 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Behavioral and Functional Characterization of Xenobiotic Responsible Enzymes in the Colorado Potato Beetle Leptinotarsa Decemlineata written by Casey Cruse and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolic detoxification is one of the major mechanisms through which insects adapt to their chemical environment. Xenobiotic responsible enzymes (XREs), including cytochrome P450s (P450s) and carboxyl/cholinesterases (CCEs) facilitate insects' adaptation to diverse xenobiotics, such as pesticides, ingested plant allelochemicals, insect and plant odors, pheromones and hormones, as well as other environmental toxins. The Colorado potato beetle (CPB), Leptinotarsa decemlineata, is a notorious pest of potatoes and other Solanaceous crops world-wide. It is well known that CPB has an exceptional ability to adapt to the toxins of its host plants, and to develop resistance to nearly all classes of pesticides. CPB is able to locate its host plants through the unique blend of volatile odors produced. Dissecting functions of XREs in the olfactory system of CPB will help us to understand the mechanisms of xenobiotic adaptation that facilitate host plant location and pesticide resistance. Here, my research focused on functional characterization of two XREs. One is an integument esterase (LdIntE) and the other is NADPH-Cytochrome P450 reductase (CPR) of L. decemlineata (LdCPR). CPR serves as a redox partner for all microsomal P450s in most eukaryotic cells. qRT-PCR revealed both XREs were highly expressed in the olfactory organs of CPB. Additionally, LdIntE could be induced 2-fold higher in beetles exposed to potato leaves and their volatiles compared to beetles fed an artificial diet. Silencing LdCPR and LdIntE separately through RNA interference (RNAi) altered the attraction of CPB to potato plants in behavior assay. Suppression of LdCPR through RNAi also increased susceptibility to imidacloprid in resistant beetles and increased their electrophysiological response to (E)-2-hexen-1-ol, and the volatile odors of potato leaves through electroantennogram. Our results suggest that LdIntE and LdCPR play vital roles in pesticide resistance, olfaction, and host plant location.
Download or read book Movement and Spatial Costs of Resistance in the Colorado Potato Beetle Leptinotarsa Decemlineata say Coleoptera Chrysomelidae written by Kathleen Schnaars Uvino and published by . This book was released on 2013 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Colorado potato beetle, Leptinotarsa decemlineata is infamous for its' ability to develop resistance to insecticides and remains the most important insect defoliator of potatoes today. Long Island populations of the Colorado potato beetle have been at the forefront of developing resistance to every newly developed insecticide. Managing the evolution of resistance requires cultural as well as chemical means. Cultural efforts include field rotation, crop rotation, chemical rotation as well as refugia. Movement plays an integral part of both, the cultural schemes intended to thwart resistance evolution and the life history traits of the Colorado potato beetle. The use of refuges and crop rotation are often promoted to supplement the use of chemical pesticides in an effort to control crop pests. Refuges are untreated areas adjacent to treated crops, where susceptible genes can survive. The efficacy of refuges depends on movement between treated and untreated areas. Differences in movement between resistant and susceptible beetles can play a big role in the success of the refuge or rotation plan. Crop rotation can reduce the amount of insecticide used through dosage levels or frequency of application and slows insects' resistance evolution. Resistance to insecticides often has fitness costs associated with that resistance. I hypothesized that resistance to the insecticide Imidacloprid is correlated with reduced movement capability in Colorado potato beetles, Leptinotarsa decemlineata (Say) (henceforth potato beetles), the primary insect defoliator of potato plants. I examined whether migratory ability or flight propensity have a cost of resistance to imidacloprid in Colorado potato beetles, Leptinotarsa decemlineata (Say) by examining LD50's of flying emergers and walking emergers in the spring. Imidacloprid is the most widely used and in some cases the only effective insecticide for Colorado potato beetle control and there is currently a wide range of variation in resistance. In the spring overwintering potato beetle adults halt diapause and emerge from overwintering sites. For the purposes of this work I will use the definition of diapause presented by Tauber et al (1986): "a neurohormonally mediated, dynamic state of minimal activity that occurs during a genetically determined stage(s) of metamorphosis, usually in response to environmental stimuli that precede unfavorable conditions." Diapause in the Colorado potato beetle begins before the harsh conditions set in (loss of host and cold temperatures). It is an important strategy employed by many temperate zone insects for overwintering. Upon emergence from the overwintering site they emigrate to colonize local and distant fields. Emergence from diapause therefore offers an opportunity to sample genetically diverse groups of beetles. My results indicate that emerging flyers have a higher level of resistance than emerging walkers from overwintering sites. I also examined populations that were under intense selection pressure from one chemical, Spinosad, and largely isolated from other fields or populations. Spinosad is produced by a soil dwelling bacterium called Saccharopolyspora spinosa and it kills by ingestion. Spinosad is currently the only approved chemical available to Organic farmers on Long Island. These results indicate complete failure of Spinosad on that population but less resistance on distant populations and less resistance on populations from conventionally managed fields, all in Suffolk County, Long Island. Additionally early spring colonists of rotated and `non-rotated' fields were evaluated for resistance levels for 3 years. For two of the three years, colonists on long distance rotated fields had high LD50. Assuming long distance colonization is more likely dependent on flight, this is consistent with my results that emerging flyers have a higher LD50 than emerging walkers.
Download or read book Reducing Insecticide Resistance of Colorado Potato Beetle written by Canada-Manitoba agreement on Agricultural Sustainabliity and published by . This book was released on 199? with total page 1 pages. Available in PDF, EPUB and Kindle. Book excerpt:
