EBookClubs

Read Books & Download eBooks Full Online

EBookClubs

Read Books & Download eBooks Full Online

Book Mechanism and Regulation of the Stress Response in Saccharomyces Cerevisiae

Download or read book Mechanism and Regulation of the Stress Response in Saccharomyces Cerevisiae written by Eric Edward Powers and published by . This book was released on 1998 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Yeast Stress Responses

    Yeast Stress Responses

    Book Details:
  • Author : Stefan Hohmann
  • Publisher : Springer Science & Business Media
  • Release : 2007-10-23
  • ISBN : 3540456112
  • Pages : 398 pages

Download or read book Yeast Stress Responses written by Stefan Hohmann and published by Springer Science & Business Media. This book was released on 2007-10-23 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.

Book Regulation of Stress Induced Gene Expression in Saccharomyces Cerevisiae

Download or read book Regulation of Stress Induced Gene Expression in Saccharomyces Cerevisiae written by Vasudha Bharatula and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription initiation is complex process involving transcription factors(TF), co-activators, nucleosome remodelers and the pre-initiation complex (PIC);general transcription factors (GTF) and RNA polymerase II (RNAP2). The spatiotemporalorganization of these different proteins and their role in regulatingplasticity and selectivity of transcriptional reprogramming in various stressesremains unknown. I describe the role of Multicopy Suppressor of Snf1 mutation(Msn2); a stress responsive transcription factor, Mediator (coactivator) andnucleosome remodeling in fine-tuning gene expression in response to stress inyeast.Msn2 exhibits distinct patterns of nucleo-cytoplasmic oscillations indifferent stresses. I determined that Msn2 target genes exhibited fast or slowinduction kinetics in response to transient and persistent Msn2 nuclearoccupancy respectively. Chromatin immunoprecipitation studies revealed thatMsn2 binds promoters of common stress genes as well as condition specificgenes in nutrient and oxidative stresses, suggesting that TF dynamics could playa role in selective gene regulation in different stresses. Additionally, Msn2binding to ~30 oxidative genes was dependent on Yap1. However, deleting Msn2did not lead to a significant decrease in expression of oxidative stress responsegenes as did the absence of Yap1. These results suggest that indirectcooperativity between Msn2 and Yap1 could lead to selective promoter bindingbut the effects of such interactions on gene expression remain unclear.I studied the role of Mediator in native conditions, where its function has not been adequately addressed. Mediator occupies chromosomal interacting domains (CID), which mark boundaries between interacting genomic regions, suggesting that Mediator could play a significant role in higher-order genome organization along with functioning as a coactivator.Finally, I assessed the combined effect of Msn2, Mediator and nucleosome remodeling on gene expression upon nutrient deprivation. Both Msn2 and Mediator are selectively recruited to promoters of genes activated and repressed in nutrient stress. Genes activated by Msn2 exhibited rapid loss of nucleosomes, in an Msn2 dependent manner. In contrast, repression of genes was not accompanied by a significant gain in nucleosomes, suggesting additional mechanisms of repression could exist. The absence of Msn2, significantly decreased Mediator recruitment at promoters of target genes, indicating that Mediator- TF interactions are crucial for gene expression changes in stress. Interestingly, both RNAP2 and Mediator remained poised at promoters of repressed genes, possibly to re-initiate transcription once the stress has passed.These findings offer novel insights on how Msn2 regulates expression of specific genes depending on the environment by modulating its dynamics and genomic binding. The binding of Msn2 helps recruit Mediator and facilitate nucleosome loss at genes induced in stress. Whereas, repressed genes seems to possess Mediator and RNAP2 in an inactive form which could be the mechanism of repression and a way to activate these genes immediate after stress.

Book Stress Response Mechanisms in Fungi

Download or read book Stress Response Mechanisms in Fungi written by Marek Skoneczny and published by Springer. This book was released on 2018-10-16 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers both the molecular basics of fungal stress response strategies as well as biotechnological applications thereof. The complex regulatory mechanisms of stress response pathways are presented in a concise and well-readable manner. Also, light will be shed on the interconnection of pathways responding to different types of stress. Profound knowledge of stress responses in yeast and filamentous fungi is crucial for further optimization of industrial processes. Applications are manifold, for example in fungicide development, for improving the resistance of crop plants to fungal pathogens, but also in medicine to help curing fungal infections. The book targets researchers from academia and industry, as well as graduate students interested in microbiology, mycology and biomedicine.

Book Mechanisms of Post Transcriptional Regulation in S Cerevisiae

Download or read book Mechanisms of Post Transcriptional Regulation in S Cerevisiae written by Vince Harjono and published by . This book was released on 2022 with total page 111 pages. Available in PDF, EPUB and Kindle. Book excerpt: Post-transcriptional regulation represents a powerful and versatile mechanism to fine-tune gene expression to meet cellular and environmental demands. One important aspect of post-transcriptional regulation involves regulation of protein translation, the process of building proteins from a messenger RNA. In this dissertation, I use biochemical and molecular biology techniques to study how translation is mechanistically regulated by both mRNA and protein factors. In chapter 2, I discuss the development of a quantitative method in eukaryotes to measure ribosomal stalls of cis-mRNA factors on protein elongation. We find that different distributions of nonoptimal codons trigger different surveillance and rescue pathways despite similar levels of elongation delay. In chapter 3, I explore the relationship between translatability and mRNA localization during glucose starvation and investigate potential factors that influence this relationship. We find that a complex made from Rvb1 and Rvb2 is involved in promoter-directed cytoplasmic fate in a subset of stress response genes in glucose starvation. Furthermore, we use carefully designed reporters to interrogate how translatability determines cytoplasmic localization and find that active translation is linked to exclusion from stress-induced cytoplasmic granules. Finally in chapter 4, I discuss improvements on the method we have developed, possible future directions for the work described in this dissertation, and my concluding remarks.

Book Protein Homeostasis

Download or read book Protein Homeostasis written by Richard I. Morimoto and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proper folding of proteins is crucial for cell function. Chaperones and enzymes that post-translationally modify newly synthesized proteins help ensure that proteins fold correctly, and the unfolded protein response functions as a homeostatic mechanism that removes misfolded proteins when cells are stressed. This book covers the entire spectrum of proteostasis in healthy cells and the diseases that result when control of protein production, protein folding, and protein degradation goes awry.

Book Biology of Microorganisms on Grapes in Must and in Wine

Download or read book Biology of Microorganisms on Grapes in Must and in Wine written by Helmut König and published by Springer. This book was released on 2017-11-01 with total page 711 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of the book begins with the description of the diversity of wine-related microorganisms, followed by an outline of their primary and energy metabolism. Subsequently, important aspects of the secondary metabolism are dealt with, since these activities have an impact on wine quality and off-flavour formation. Then chapters about stimulating and inhibitory growth factors follow. This knowledge is helpful for the growth management of different microbial species. The next chapters focus on the application of the consolidated findings of molecular biology and regulation the functioning of regulatory cellular networks, leading to a better understanding of the phenotypic behaviour of the microbes in general and especially of the starter cultures as well as of stimulatory and inhibitory cell-cell interactions during wine making. In the last part of the book, a compilation of modern methods complete the understanding of microbial processes during the conversion of must to wine.This broad range of topics about the biology of the microbes involved in the vinification process could be provided in one book only because of the input of many experts from different wine-growing countries.

Book Gene Regulation During Stress Response Transcription in Saccharomyces Cerevisiae

Download or read book Gene Regulation During Stress Response Transcription in Saccharomyces Cerevisiae written by Christian Miller and published by . This book was released on 2013 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Molecular Aspects of the Stress Response Chaperones Membranes and Networks

Download or read book Molecular Aspects of the Stress Response Chaperones Membranes and Networks written by Peter Csermely and published by Springer Science & Business Media. This book was released on 2007-08-09 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book makes a novel synthesis of the molecular aspects of the stress response and long term adaptation processes with the system biology approach of biological networks. Authored by an exciting mixture of top experts and young rising stars, it provides a comprehensive summary of the field and identifies future trends.

Book Dynamic Signaling and Adaptation in Saccharomyces Cerevisiae Stress Response Pathways

Download or read book Dynamic Signaling and Adaptation in Saccharomyces Cerevisiae Stress Response Pathways written by Zohreh AkhavanAghdam and published by . This book was released on 2017 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt: Living cells respond to environmental cues through complex signaling and gene regulatory networks. A common theme throughout this thesis will be exploring design principles in biological networks and how they operate dynamically to process information and make decisions. In Chapter 1, we tackle how different types of stresses induce distinct nuclear translocation dynamics of Msn2, an outstanding question in the field. In the absence of stress, PKA phosphorylates Msn2, causing it to be exported out of the nucleus. In response to stress, PKA activity is inhibited, Msn2 is dephosphorylated and translocated into the nucleus. In response to glucose limitation, Msn2 exhibits an initial homogenous pulse of nuclear translocation followed by sporadic nuclear pulses with dose-dependent frequency, but in response to osmotic stress Msn2 undergoes a single translocation pulse with dose-dependent duration. We hypothesized that the difference between glucose limitation and osmotic stress-induced Msn2 dynamics might be a result of glucose limitation-dependent Snf1 activation, since previous studies suggest that Snf1 and PKA mutually inhibit each other. We use modeling and experiments to demonstrate that these different upstream network structures could, in fact, be responsible for the differences we see in Msn2 translocation dynamics. In Chapter 2, we study a recurring scheme in gene regulatory networks, which is combinatorial gene regulation by seemingly redundant transcription factors (TFs), using time-lapse microscopy and microfluidics. We use the seemingly redundant yeast homologous stress responsive TFs Msn2 and Msn4 as a model to quantitatively study the functional relevance of closely related TFs in the same single cells and find that Msn2 and Msn4 have non-redundant and distinct functions in combinatorial gene regulation. In response to a transient input, either Msn2 or Msn4 alone is sufficient to induce the expression of target genes with fast kinetics promoters. Target genes with slow kinetics promoters, however, require activation of both Msn2 and Msn4 in these conditions. Importantly, slow kinetic promoter activation is dependent on duration of the upstream signal because in response to a prolonged input, slow kinetic promoter activation no longer requires both Msn2 and Msn4. Thus, in Chapter 2, we determine that coordinated gene regulation by seemingly redundant TFs is not fixed, but rather dependent on the dynamics of upstream signals. In Chapter 3, we demonstrate that cells retain a memory of many of upstream signaling events that occur in response to stress, which primes the cells to respond to future severe stress events. We use microfluidics and time-lapse microscopy to modulate the amplitude and duration of priming stimulus and also increase the break time in between the priming stimulus and severe stress. Using this system, we have determined that cells acquire an amplitude-dependent short-term memory of priming stimulus, which is induced and lost rapidly, and a duration-dependent long-term memory which is stable for a long period of time before finally declining after 100 minutes. We use this information about the dynamical specificity of different types of cellular memory and their stability to determine the cellular pathways responsible for the observed memory.

Book Termination of Replication Stress Signaling in Saccharomyces Cerevisiae

Download or read book Termination of Replication Stress Signaling in Saccharomyces Cerevisiae written by Carolyn Marie Jablonowski and published by . This book was released on 2016 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book The Molecular Biology of Schizosaccharomyces pombe

Download or read book The Molecular Biology of Schizosaccharomyces pombe written by Richard Egel and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fission yeast Schizosaccharomyces pombe is the favoured tool of many productive research groups throughout the world, serving as a useful model for fundamental principles and mechanisms, such as genome organization, differential gene regulation, cell-cycle control, signal transduction, or cellular morphogenesis. This book collates the current state of knowledge derived from molecular studies in this simple eukaryotic microorganism. The entire sequence of its genome has been completed, emphasizing the comparative value and model status of this yeast. The individual chapters, highlighting up-to-date views on prominent aspects of molecular organization, were written by active research scientists, presenting the results of their investigations to other workers in neighbouring fields. This book intends to serve the fission yeast community as a handy source of reference for years to come. It will also be of particular value to the ever-increasing number of researchers starting to look into fission yeast affairs for comparative reasons from other platforms of molecular genetics and cell biology.