Download or read book Measuring Cell Adhesion written by A. S. G. Curtis and published by Wiley. This book was released on 1991-09-05 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: A compilation and evaluation of research work and methods in the field of cell adhesion. It is the work of biologists and biophysicists interested primarily in the study of animal cells. However, the methods described can be extended to microbial or plant cells and to the study of interactions in the non-living world. It has origins in a workshop held to compare laboratory methods on the subject.
Download or read book Physical Basis of Cell Cell Adhesion written by Pierre Bongrand and published by CRC Press. This book was released on 2018-02-01 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Present book is aimed at providing a readable account of physical methods and results required to measure cell adhesion and interpret experimental data. Since on the one hand readability seemed a major quality for a book, and on the other hand, the problems posed referred to a wide range of domains of physics, chemistry, and biology, completeness had to sacrificed. Indeed, a whole book would not suffice to quote the relevant literature (and many more authors would be required to have read it). Hence, only a limited number of topics were selected for reliability of methods, availability of enough experimental results to illustrate basic conception or potential use in the future. These were discussed in three sections.
Download or read book Measuring Cell Mechanics written by Margaret Gardel and published by Biota Publishing. This book was released on 2015-09-01 with total page 77 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells are inherently physical entities that both experience mechanical forces from their external environment and generate their own internal forces to drive cell motion. Our particular aim here is to present the reader with an introduction to the primary tools used to measure these mechanical interactions and the material properties of cells that result from them. These approaches can be applied to a diverse array of physiological processes and systems, providing important insight into the regulatory roles of mechanical interactions in cells. We cover techniques at both the molecular and cellular scales, including those that actively and passively probe the system. Along the way we cover the fundamental principles of each approach, while emphasizing the relevant length and timescales, along with the typical magnitudes of the measurements that can be made. Each section ends by highlighting uses of the various techniques in recent relevant publications, illustrating the exciting future of cell mechanics in quantitative cell biology research.
Download or read book Characterization of Biomaterials written by Susmita Bose and published by Elsevier Inc. Chapters. This book was released on 2013-03-12 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: This brief introductory chapter provides a broad overview of materials, biomaterials and the need to understand different techniques to characterize biomaterials. From this chapter, the reader can gain a perspective on how the rest of the topics in different chapters are divided to fully comprehend this inherently multidisciplinary field. Application of appropriate characterization tools can not only save time to fully evaluate different biomaterials, it can also make commercial biomedical devices safer. In the long run, safer biomedical devices can only reduce the pain and suffering of mankind, a dream that resonates with every biomedical researcher.
Download or read book Physics of Cancer written by Claudia Mierke and published by Iph001. This book was released on 2018-10-24 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: This revised second edition is improved linguistically with multiple increases of the number of figures and the inclusion of several novel chapters such as actin filaments during matrix invasion, microtubuli during migration and matrix invasion, nuclear deformability during migration and matrix invasion, and the active role of the tumor stroma in regulating cell invasion.
Download or read book Characterization of biomaterials written by N.D. Gallant and published by Elsevier Inc. Chapters. This book was released on 2012-12-19 with total page 31 pages. Available in PDF, EPUB and Kindle. Book excerpt: This chapter reviews the quantification of cell adhesion and motility with a focus on the assays that enable these analyses. A description of these critical biological processes is provided and the characteristic parameters are described. Each assay is then classified according to the metrics used to quantify cell adhesion or locomotion. Finally, some recent advances and future prospects for the field are presented.
Download or read book Studying Cell Adhesion written by Pierre Bongrand and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell adhesion - the attachment of cells to any surface such as other cell membranes or tissues - is a complex process. In many physiological and pathological processes adhesion of a cell is the first critical step. A wide spectrum of the most powerful techniques currently available to study the basic parameters of cell adhesion, including binding strength, binding efficiency, membrane-membrane or membrane-substrate interaction, structural properties and dynamics of cell surface molecules, is presented in this strategy book. Sophisticated quantitative approaches as well as comprehensible semi-quantitative methods are described. The detailed theoretical background allows the critical assessment and application of these techniques.
Download or read book Measurement of Cell Adhesion Forces by Holographic Microscopy written by Stanislaw Makarchuk and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mechanical forces, generated by the cell plays crucial role in cell adhesion - common process for different cell lines. ln order to measure the force map during cellular adhesion, we use Traction Force Microscopy (TFM), where cell adheres to the soft substrate in 20 plane, and the forces are calculated from measured displacement field inside the substrate underneath the cell. We built the microscope, where instead of using fluorescent markers, we use spherical polystyrene beads in order to measure the displacement field. Positions of the markers are obtained by analyzing the interference pattern caused by the beads in bright-field light. With this technique, we reach nanometer accuracy of the microsphere position determination, that, respectively, influence accuracy of the calculated force field. With the microscope first measurements were performed with cancer cell line SW 480.
Download or read book Adhesion of Cells Viruses and Nanoparticles written by Kevin Kendall and published by Springer Science & Business Media. This book was released on 2010-11-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Adhesion of Cells, Viruses and Nanoparticles" describes the adhesion of cells, viruses and nanoparticles starting from the basic principles of adhesion science, familiar to postgraduates, and leading on to recent research results. The underlying theory is that of van der Waals forces acting between cells and substrates, embodied in the molecules lying at the surfaces, together with the geometry and elasticity of the materials involved. The first part describes the fundamental background to adhesion principles, including the phenomenology, the important equations and the modeling ideas. Then the mechanisms of adhesion are explored in the second part, including the elastic deformations of spheres and the importance of the energy of adhesion as measured in various tests. It is demonstrated that adhesion of cells is statistical and depends on Brownian movement and on the complex multiple contacts that can form as cells move around. Then, detailed chapters on cell adhesion, contact of viruses and aggregation of nanoparticles follow in Part 3. Finally, the last chapter looks to the future understanding of cell adhesion and points out some interesting directions of research, development and treatment of diseases related to these phenomena. This book is an ideal resource for researchers on adhesion molecules, receptors, cell and tissue culturing, virus infection, toxicity of nanoparticles and bioreactor fouling. It can also be used to support undergraduate and Masters level teaching courses. "This is a fascinating book and it is an invaluable resource for understanding particle-particle/surface adhesion at micro- and nano- scales. I intend to keep one for my future reference and highly recommend it to my students." (Prof. Zhibing Zhang, School of Chemical Engineering, University of Birmingham, UK)
Download or read book Cell Adhesion Molecules written by Martin E. Hemler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Fourth Annual Pezcoller Symposium entitled Adhesion Molecules: Cellular Recognition Mechanisms was held in Rovereto, Italy, June 24-26, 1992 and was focussed on the detailed mechanisms whereby cells utilize certain integral membrane proteins to perceive their surrounding environment and interact with it. With timely presentations and stimulating discussions this Symposium addressed the genetics and biochemistry of adhesion molecules, the regulation of their functions and their role in cancer and the immune system. Emphasis was given to adhesion proteins in the integrin family because of the widespread distribution of this group of molecules and its important role in essentially all eukaryotic biological systems. The regulation of integrin genes and their expression are discussed in detail, as are specific aspects of the genetics of fibronectin. The molecular basis for the regulation of certain integrins, the function of these proteins in determining cell adhesion, and the consequences of this adhesion for the function of the cells involved are discussed. The role of certain integrins in stimulating signal transduction, the essential involvement of integrins in conditioning the function of T and NK cells function, the heterogeneity of integrins and its biological consequences, and the role of cell adhesion molecules in tumor cells invasion and metastases are all extensively analyzed. New information was presented on the role of CD44 and splice variants in normal differentiation and tumor progression.
Download or read book Surface and Interfacial Aspects of Cell Adhesion written by Alain Carré and published by CRC Press. This book was released on 2011-01-19 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell adhesion comes into play in almost all domains of life. The range of situations in which it occurs, involving organisms, living tissues, microorganisms or single cells, is endless. Cell adhesion is involved in the binding of a cell to a surface, extracellular matrix, or another cell using cell adhesion molecules. It is crucial in the formation and maintenance of coherent multicellular structures. Cell surface adhesion molecules (integrins, for example) which transmit information from the extracellular matrix to the cell play vital roles in numerous cellular processes. Some of these include: cell growth, differentiation, embryogenesis, immune cell transmigration and response, and cancer metastasis. Also cell adhesion is involved in most of pathological situations. This book is divided into four parts as follows: Part 1: Fundamentals of Cell Adhesion; Part 2: Methods to Study Cell Adhesion; Part 3: Surface Treatments to Control Cell Adhesion and Behavior; and Part 4: Cell Adhesion in Medicine and Therapy. A bountiful information is covered in this book which represents the cumulative wisdom of many world-renowned researchers( physicists, materials scientists, chemists and biologists) engaged in unraveling the mechanisms of cell adhesion and how to mitigate or control it. It quite patent from the topics covered in this book that the subject of cell adhesion is truly interdisciplinary. This book should be of great interest and value to anyone interested in cell adhesion which is vitally important to human life.
Download or read book Single Cell Analysis in Biotechnology and Systems Biology written by and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annotation Cells play significant roles in our day to day life. However, the interactions of cells, the cellular responses of organelles to molecules, and their intracellular behaviour, are still not fully understood. To better understand the physiological interactions among molecules, organelles, and cells, the ensemble measurement of (on average, millions of) cells cannot provide detailed information. However, for example, research concerning the differentiation behaviors of stem cells or the metastatic processes of tumour initiation requires detailed information. Understanding genomic sequence information at a single cell level can promote an understanding of how individual parts of a cell are integrated in time and space to form dynamic cellular processes. The relationship between cellular heterogeneity and signaling pathway regulation may result in an understanding of disease states that can potentially drive therapeutic interventions. Thus single cell analysis (SCA) has been emerging as a powerful method of investigating exciting new insights into genomics, fluxomics, proteomics, and systems biology.
Download or read book Methods in Cell Matrix Adhesion written by Josephine Adams and published by Academic Press. This book was released on 2002-06-12 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual background for all aspects of cell-matrix adhesion research: the extracellular matrix, adhesion receptors, and the growing number of functional applications of matrix-adhesion in molecular cell biology. Also covered is the purification of the extracellular matrix to functional analyses of cellular responses.
Download or read book Physics of Bio Molecules and Cells written by Henrik Flyvbjerg and published by Springer Science & Business Media. This book was released on 2003-07-01 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aimed at those working to enter this rapidly developing field, this volume on biological physics is written in a pedagogical style by leading scientists giving explanations that take their starting point where any physicist can follow and end at the frontier of research in biological physics. These lectures describe the state-of-the-art physics of biomolecules and cells. In biological systems ranging from single biomolecules to entire cells and larger biological systems, it focuses on aspects that require concepts and methods from physics for their analysis and understanding, such as the mechanics of motor proteins; how the genetic code is physically read and managed; the machinery of protein--DNA interactions; force spectroscopy of biomolecules' velopes, cytoskeletons, and cytoplasms; polymerization forces; listeria propulsion; cell motility; lab-on-a-chip nanotechnology for single-molecule analysis of biomolecules; bioinformatics; and coding and computational strategies of the brain.
Download or read book Integrin and Cell Adhesion Molecules written by Motomu Shimaoka and published by Humana Press. This book was released on 2011-09-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Direct Measurements of Cell Adhesion Forces written by Geoffrey William Francis and published by . This book was released on 1993 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
