Download or read book Mass Spectrometry of Non Covalent Complexes written by Christoph A. Schalley and published by John Wiley & Sons. This book was released on 2009-09-08 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Details the many benefits of applying mass spectrometry to supramolecular chemistry Except as a method for the most basic measurements, mass spectrometry (MS) has long been considered incompatible with supramolecular chemistry. Yet, with today's methods, the disconnect between these two fields is not warranted. Mass Spectrometry and Gas-Phase Chemistry of Non-Covalent Complexes provides a convincing look at how modern MS techniques offer supramolecular chemists a powerful investigatory toolset. Bringing the two fields together in an interdisciplinary manner, this reference details the many different topics associated with the study of non-covalent complexes in the gas phase. The text begins with brief introductions to supramolecular chemistry and such relevant mass spectrometric methods as ionization techniques, analyzers, and tandem MS experiments. The coverage continues with: How the analyte's transition into the gas phase changes covalent bonding How limitations and pitfalls in analytical methods may produce data misinterpretations Artificial supramolecular aggregates and their examination Biomolecules, their complexes, and their examination After the general remarks making up the first section of the book, the following sections describe specific experimental procedures and are illustrated with numerous examples and short tutorials. Detailed citations end each chapter. Mass spectrometrists, supramolecular chemists, students in these fields, and interested readers from other disciplines involving the study of non-covalent bonds will all value Mass Spectrometry and Gas-Phase Chemistry of Non-Covalent Complexes as an innovative and practical resource.
Download or read book Dissecting Non covalent Interactions in Oligomeric Protein Complexes by Native Mass Spectrometry written by Katharina Root and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Approaches to the Stabilization of Non covalent Complexes in Matrix assisted Laser Desorption ionization Mass Spectrometry written by Anne Marie Distler and published by . This book was released on 2003 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Study of Non covalent Protein Complexes by Electrospray Ionization Mass Spectrometry written by Jiangxiao Sun and published by . This book was released on 2009 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Non covalent Complexes by Electrospray Ionization ESI Fourier Transform Mass Spectrometry FTMS written by Fei He and published by . This book was released on 1998 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The study of non covalent complexes in a quadrupole ion trap by electrospray ionization mass spectrometry written by Esther Caroline Kempen and published by . This book was released on 2000 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrospray Mass Spectrometry written by Norman K. Sproch and published by . This book was released on 1994 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Tandem Fourier transform Mass Spectrometry of Noncovalent Complexes of Biological Ions written by John Clement Jurchen and published by . This book was released on 2003 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chiral Studies of Non covalent Complexes by Electrospray Ionization ESI Fourier Transform Mass Spectrometry FTMS written by Gabriela Grigorean and published by . This book was released on 2002 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrospray Ionization Mass Spectrometry Analysis of Covalent and Non covalent DNA Complexes written by Sarah Elizabeth Pierce and published by . This book was released on 2010 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: The covalent and non-covalent interactions between DNA and external ligands and between DNA and itself are critical for cellular function. An increased knowledge of these interactions can be used for the development of disease-fighting agents, specifically anti-cancer drugs with improved sensitivity and specificity for tumor cells. Electrospray ionization mass spectrometry (ESI-MS) is useful in the screening and characterization of the interactions involving nucleic acids given the speed and small sample sizes that can be analyzed. In this dissertation, ESI-MS is used to characterize covalent and non-covalent interactions involving DNA to assist in determining how these interactions can lead to better therapeutics. The non-covalent binding of ligands to quadruplex oligonucleotides is discussed first. Pyrrole inosine ligands, which bind to guanine bases, were found to interact with both quadruplexes and with guanine rich oligonucleotides without a quadruplex structure. While those interactions were specific with guanine, novel platinum complexes were found to form specific interactions with quadruplex structures themselves as the size of the ligands matched the size of a guanine quartet. This allowed the ligands to end-stack with quadruplexes with large thymine-rich loops between guanine-rich regions. The non-covalent and covalent interactions between ligands and other DNA structures were also studied. The non-covalent binding of anthracycline ligands to mismatched DNA hairpins was probed. The analysis of solutions of approximately equimolar ligand and oligonucleotide indicated preferential binding to the mismatched sequences. Diazirdinyl benzoquinone crosslinkers, including the clinically studied RH1 and an analogue of RH1, were reacted with a variety of duplex oligonucleotides. The complexes were observed by LC-MS and dissociated using both CID and IRMPD to determine the sites of crosslinking. It was determined that both ligands could form interstrand crosslinks in DNA with 5'-GNC or 5'-GNNC sequences. The RH1 analogue, with a bulky phenyl group, formed fewer crosslinks than RH1. In addition to studying DNA/ligand interactions, the interactions between oligonucleotides were also probed. Oligonucleotides containing non-standard isoguanine repeats were annealed in the presence of various cations to determine how those cations would affect the resulting secondary structures. In most cases, isoguanine containing strands formed pentaplexes rather than quadruplexes, which were observed for strands containing guanine bases.
Download or read book Investigation of Noncovalent Complexes with Soft Ionization Mass Spectrometry written by Arno Wortmann and published by . This book was released on 2007 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrospray Mass Spectrometry written by Shadrack Osei Nkansah and published by . This book was released on 1996 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanoelectrospray Mass Spectrometry for Molecular Non covalent Interactions and Proteomics written by and published by . This book was released on 2006 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry has become an indispensable tool in biological analysis. Electrospray ionization is an important tool in the study and characterization of non-covalent molecular complexes because it is a soft ionization technique that preserves non-covalent binding stoichiometries. Nanoelectrospray, a miniaturized electrospray with improved ion transfer efficiency, is well-suited for the study of molecular non-covalent interactions. Polyaniline and graphite coated nanoelectrospray emitters, have long lifetimes and are utilized in the nanoelectrospray source coupled with commercial MS for the non-covalent interactions analysis and proteomics. Solution and gas-phase hydrogen/deuterium (H/D) exchange are promising methods in elucidating the solution or gas phase formation of non-covalent complexes. The H/D exchange technique is commonly used to provide insights into the roles of various non-covalent interactions in stabilizing the appropriate three-dimensional (3-D) structural information of solution and gaseous ions, including information on protein. We elucidated the mechanism of disulfide-bond reaction, and the specific role of hydrogen bonds in the formation of the disulfide linkages. This system provides an ideal platform to investigate the evolution of proteins. Additionally, the dual-functional nanoemitters are fabricated by immobilizing the trypsin onto the inner wall of the fused silica capillaries which are utilized as the nanoemitters with polyaniline coating as well. The diluted protein samples can be fast digested on line and are analyzed by the mass spectrometer without desalting. In general, this dissertation demonstrated the development of nanoelectrospray mass spectrometry for molecular non-covalent interactions and proteomics.
Download or read book Study of Non covalent Protein hydrophobic Ligand Complexes by Electrospray Ionization Mass Spectrometry written by Lan Liu and published by . This book was released on 2011 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mass Spectrometry in Biology Medicine written by A.L. Burlingame and published by Springer Science & Business Media. This book was released on 1999-09-14 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading practitioners detail revolutionary new spectrometric techniques for the identification and covalent structural characterization of macromolecules, proteins, glycoconjugates, and nucleic acids. Based on the Fourth International Symposium on Mass Spectrometry in the Health and Life Sciences held in San Francisco in 1998, this invaluable book contains tested strategies for solving many significant biomedical research problems. The techniques use mass spectrometry, automated computer processing of spectral information, and gene, protein, and EST databases for genomic and proteomic correlations. Mass Spectrometry in Biology and Medicine offers a unique opportunity to explore and apply these new techniques of mass spectrometry that are revolutionizing the identification and structural characterization of proteins, carbohydrates, and nucleic acids.
Download or read book The Investigation of Noncovalent Complexes Detected by Fast Atom Bombardment and Electrospray Mass Spectrometry written by John B. Cunniff and published by . This book was released on 1995 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Probing the Stability Structure and Reactivity of Noncovalent Complexes of Biological Interest Using Ion Trap Mass Spectrometry written by Emily Elizabeth Jellen and published by . This book was released on 2004 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
