Download or read book Mass Spectrometry based Strategies for Biomolecular Structure Analysis written by Yuetian Yan and published by . This book was released on 2015 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry is an important method for studying the structure of both small molecules and large biomolecules (e.g., proteins). The majority of the applications prior to 1970 were focused on small molecules, owing to the limited ionization methods which posed difficulties in producing gas-phase ions for large biomolecules then. Beginning in the 1980's, with the introduction of new ionization methods (ESI and MALDI), the applications have gradually switched to biological science measuring large bioorganic molecules. Today, with the developing interest in metabolomics and proteomics, and ongoing improvement in MS-based techniques, mass spectrometry is extensively applied in the study of both small and large molecules. The research presented in this thesis falls into two main parts, which focus on the application of MS in (1) structural analysis of steroid metabolites and (2) characterization of protein-protein interactions. In the first part, combinations of different MS methods are adopted and used to solve the structures of unknown steroid metabolites, which are the pheromones responsible for mouse communication in mouse urine. This part includes three chapters, the first two of which discuss the method development of using MS to study the structure of steroid metabolites; and the third chapter presents the application of the MS methods in solving a newly discovered steroid pheromone, which is determined as a sex-specific hormone. In the second part, two MS-based strategies, namely, hydrogen-deuterium exchange (HDX) and fast photochemical oxidation of proteins (FPOP), are applied in two studies of protein-protein interactions, including: (1) dimerization of SecA, which is a motor protein in bacteria translocation pathway; and (2) interface mapping of EGFR binding to Adnectin1. In the first chapter in Part 2, we used HDX MS to characterize the dimer interface of SecA, and, meanwhile, detected a conformational change from open to closed forms at the pre-protein binding domain upon dimerization. This conformational change provided leads for the active form of SecA. In the second chapter in Part 2, we applied FPOP, which is modified to suit therapeutic protein formulation conditions, to map the epitope of Adnectin1-EGFR interaction at amino acid residue level. The epitope identified agrees with that from both HDX study and crystallography results, presenting more evidence of the capability of FPOP in epitope mapping. These five studies on characterization of steroid metabolites and protein-protein interactions show the successful application of mass spectrometry in the structural study of both small molecules and large proteins. Furthermore, there's a great potential for study of more complex systems.

Download or read book Mass Spectrometry in Biophysics written by Igor A. Kaltashov and published by John Wiley & Sons. This book was released on 2005-05-06 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical background and technical aspects of eachmethod, this much-needed reference offers an unparalleled overviewof the current state of biophysical MS. Mass Spectrometry inBiophysics begins with a helpful discussion of general biophysicalconcepts and MS-related techniques. Subsequent chaptersaddress: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-basedmethods * Techniques used to study structure and behavior of non-nativeprotein states that become populated under denaturingconditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information onprotein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physicsof electrospray mass spectrometry, Mass Spectrometry in Biophysicsalso offers a compelling look at the current challenges facingbiomolecular MS and the potential applications that will likelyshape its future.

Download or read book Mass Spectrometry in Structural Biology and Biophysics written by Igor A. Kaltashov and published by John Wiley & Sons. This book was released on 2012-04-03 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive guide to mass spectrometry techniques in biology and biophysics The use of mass spectrometry (MS) to study the architecture and dynamics of proteins is increasingly common within the biophysical community, and Mass Spectrometry in Structural Biology and Biophysics: Architecture, Dynamics, and Interaction of Biomolecules, Second Edition provides readers with detailed, systematic coverage of the current state of the art. Offering an unrivalled overview of modern MS-based armamentarium that can be used to solve the most challenging problems in biophysics, structural biology, and biopharmaceuticals, the book is a practical guide to understanding the role of MS techniques in biophysical research. Designed to meet the needs of both academic and industrial researchers, it makes mass spectrometry accessible to professionals in a range of fields, including biopharmaceuticals. This new edition has been significantly expanded and updated to include the most recent experimental methodologies and techniques, MS applications in biophysics and structural biology, methods for studying higher order structure and dynamics of proteins, an examination of other biopolymers and synthetic polymers, such as nucleic acids and oligosaccharides, and much more. Featuring high-quality illustrations that illuminate the concepts described in the text, as well as extensive references that enable the reader to pursue further study, Mass Spectrometry in Structural Biology and Biophysics is an indispensable resource for researchers and graduate students working in biophysics, structural biology, protein chemistry, and related fields.

Download or read book Clinical Applications of Mass Spectrometry in Biomolecular Analysis written by Uttam Garg and published by Springer Nature. This book was released on 2022-09-20 with total page 541 pages. Available in PDF, EPUB and Kindle. Book excerpt: This updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.

Download or read book Introduction to Mass Spectrometry of Biomolecules written by Marko Jovanović and published by Nova Publishers. This book was released on 2016 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: The theses, lectures and solved problems within this book give an introduction to principles of mass spectrometry related to the analysis of biomolecules. The book focuses on MS and MS/MS analysis of peptides, oligosaccharides, lipids, glycoconjugates and polyphenols. The basic properties of modern MS ionization techniques and mass analyzers are presented, as well as appropriate MS methods for each class of biomolecules in modern biomedical scientific research. The biggest part of this book is focused on MS/MS analysis of biomolecules: how the MS and MS/MS data are generated, what are the basic strategies for their structural analysis, as well as how to interpret the respective MS and MS/MS data. This book also covers principles and examples of advanced MS/MS analyses, serving as a platform towards an intermediate level of understanding scientific research carried out in the field of structural MS analysis of biomolecules. It is the author's hope that the reader will be able to understand principles of MS analyses related to their scientific and/or analytical interest, as well as design/help to propose MS and MS/MS experiments, as well as interpret the obtained data. This book is primarily written for students with basic knowledge of general, organic and analytical chemistry, and who are interested in applying MS-based methodologies in biomedical research fields, as well as interested in pursuing a career in mass spectrometry. Therefore it is best suited for senior undergraduate students and first/second year Ph.D. students. However, since MS instrumentation is rapidly adopted on all levels of scientific as well as analytical laboratories, the book should prove useful to scientists -- as well as analysts -- interested in applying MS in their upgraded laboratories. The author hopes that the reader, after studying the content presented, will be able to read primary scientific literature containing MS data to learn more about specific topics of interest, as well as be more capable of choosing a MS-related career path. For senior scientists, this book can offer some basic insights related to choosing a post-doc laboratory, or simply easier and more fruitful cooperation with mass spectrometrists.

Download or read book Analyzing Biomolecular Interactions by Mass Spectrometry written by Jeroen Kool and published by John Wiley & Sons. This book was released on 2015-05-04 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.

Download or read book Sample Preparation in Biological Mass Spectrometry written by Alexander R. Ivanov and published by Springer Science & Business Media. This book was released on 2011-06-15 with total page 1081 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the latest inventions and introduction of highly sophisticated mass spectrometry equipment sample preparation becomes an extremely important bottleneck of biomedical analysis. We have a goal of giving the reader several successful examples of sample preparation, development and optimization, leading to the success in analytical steps and proper conclusions made at the end of the day. This book is structured as a compilation of contributed chapters ranging from protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.

Download or read book Advanced Fragmentation Methods in Biomolecular Mass Spectrometry written by Frederik Lermyte and published by Royal Society of Chemistry. This book was released on 2020-12-11 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: Breaking down large biomolecules into fragments in a controlled manner is key to modern biomolecular mass spectrometry. This book is a high-level introduction, as well as a reference work for experienced users, to ECD, ETD, EDD, NETD, UVPD, SID, and other advanced fragmentation methods. It provides a comprehensive overview of their history, mechanisms, instrumentation, and key applications. With contributions from leading experts, this book will act as an authoritative guide to these methods. Aimed at postgraduate and professional researchers, mainly in academia, but also in industry, it can be used as supplementary reading for advanced students on mass spectrometry or analytical (bio)chemistry courses.

Download or read book Selected Topics in Mass Spectrometry in the Biomolecular Sciences written by Richard M. Caprioli and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many fundamental aspects of the methods used in mass spectrometry are here presented by outstanding scientists, with reference to very recent developments. The principles and applications of electrospray, ion spray and MALDI ionization technique are presented, together with optimised GC/MS interfacing systems and tools for quantitative analysis. A comprehensive treatment of modern instrumentation for mass analysis and detection is also included. The major part of the book deals with bioanalytical applications to peptides, proteins, oligonucleotides, polysaccharides, lipids and plant metabolites. Several papers are devoted to the evaluation of adduct formation between DNA and carcinogens. Environmental applications are also included, with examples of some specific cases. Fundamentals and applications are treated with the same degree of depth: the first two parts of the book therefore provide a basis for the understanding of the biomolecular applications section. Audience: Ideal for advanced graduate students of chemistry who have learned some basic mass spectrometry. Also useful for Ph.D. students in chemistry, biology and medicine. Of value to researchers in academic and industrial laboratories.

Download or read book Mass Spectrometry Analysis for Protein Protein Interactions and Dynamics written by M. Chance and published by John Wiley & Sons. This book was released on 2008-09-22 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins. Preliminary chapters cover mass spectrometry methods for examining proteins and are then followed by chapters devoted to presenting very practical, how-to methods in a detailed way. Includes footprinting and plistex specifically, setting this book apart from the competition.

Download or read book Developing Mass Spectrometry based Strategies for the Enhanced Characterization of Biomolecules in the Nervous System written by Ashley Phetsanthad and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neuronal signaling is essential for the body to recognize and respond to stimuli, both internal and external. Neurons rely on proteins, neuropeptides, lipids, and other small molecules to properly receive and transmit signals; however, this can be affected or disrupted due to changes in structure, regulation, or the presence of any post-translational modifications (PTMs). Awareness of modification states and biomolecule dysregulation is critical to understanding the molecular mechanisms involved with basic biological function, as well as those altered by various neurological diseases and disorders. While elucidating PTM states can be difficult due to heterogeneity and low stoichiometry, the sensitivity and rapid data acquisition of mass spectrometry (MS) provides the ability for quantitation and accurate PTM characterization. This dissertation is devoted to addressing the gaps in MS method development and characterization for biomolecules related specifically to the nervous system. Firstly, I describe the utilization of definitive screening designs to improve data acquisition methods for the identification of neuropeptide signaling molecules by library-free data-independent acquisition MS. While data acquisition methods are required for successful characterization by MS, the analysis of the data is crucial as well. A post-processing data workflow was developed to capture a comprehensive summary of the gas phase structure of native proteins by ion mobility MS. This was applied to amplify discrete changes in protein structure, caused by PTMs, that we found leads to the increased cytotoxicity of AÎø, a protein associated with the neurodegenerative disease, Alzheimer's disease. As the state of glycosylation, a complex heterogeneous PTM, largely affects biomolecular properties and function, characterization of altered structures is critical to understand their roles in molecular mechanisms. A portion of this thesis describes the improvement of a workflow designed for increased discovery and characterization of glycosylated neuropeptides in Callinectes sapidus, a model organism for neurophysiology studies. Additional work includes the first investigation into the altered glycoproteome associated with a fragile X syndrome (FXS) mouse model. While many other neurological disorders are associated with aberrant glycosylation, the altered expression in the brain of affected individuals was yet to be probed. Efforts to characterize the dysregulation of synaptosome glycoproteins, more directly related to synaptic plasticity and neuronal signaling, resulting from FXS are described as well. This dissertation details the development of MS methodologies to improve our understanding of the endogenous peptides and proteins involved in neuronal signaling. It is my goal that the research and results described will contribute to a better understanding of the complex processes involved with neuroscience research.

Download or read book Mass Spectrometry in Biomolecular Sciences written by Richard M. Caprioli and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry in the Biological Sciences covers the most recent technological and applied developments in the area, including both ionization techniques and ion analysis. It introduces and reviews some of the newer ionization methods, describes the major instrumentation involved in mass analysis, and presents the scope of the technology in biology, medicine, and environmental science. Specific examples are given for a number of topics. It also deals with recent achievements in the on-line combination of separation techniques such as gas chromatography, liquid chromatography, and supercritical fluid technology.

Download or read book Analysis of Protein Post Translational Modifications by Mass Spectrometry written by John R. Griffiths and published by John Wiley & Sons. This book was released on 2016-10-12 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation Discussion of the chemistry behind each modification, along with key methods and references Contributions from some of the leading researchers in the field A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research

Download or read book Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics written by Prakash Saudagar and published by Elsevier. This book was released on 2022-09-28 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics presents the latest emerging technologies in spectroscopy and advances in established spectroscopic methods. The book presents a guide to research methods in biomolecular spectroscopy, providing comprehensive coverage of developments in the spectroscopic techniques used to study protein structure and dynamics. Seventeen chapters from leading researchers cover key aspects of spectroscopic methods, with each chapter covering structure, folding, and dynamics. This title will help researchers keep up-to-date on the latest novel methods and advances in established methods. Presents current, emerging, and evolving advances and applications of spectroscopic techniques in the study of biomolecules, including proteins and nucleic acids Discusses contemporary spectroscopic techniques used to study biomolecular structure, interaction, and dynamics

Download or read book Principles of Mass Spectrometry Applied to Biomolecules written by Chava Lifshitz and published by Wiley-Interscience. This book was released on 2006-10-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: An extensive compilation of articles by leading professionals, this reference explains the fundamental principles of mass spectrometry as they relate to the life sciences. Topics covered include spectroscopy, energetics and mechanisms of peptide fragmentation, electron capture dissociation, ion-ion and ion-molecule reactions, reaction dynamics, collisional activation, soft-landing, protein structure and interactions, thermochemistry, and more. The book empowers readers to develop new ways of using these techniques.

Download or read book Introduction to Mass Spectrometry written by J. Throck Watson and published by John Wiley & Sons. This book was released on 2013-07-09 with total page 972 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised and updated, this text provides an easy-to-read guide to the concept of mass spectrometry and demonstrates its potential and limitations. Written by internationally recognised experts and utilising "real life" examples of analyses and applications, the book presents real cases of qualitative and quantitative applications of mass spectrometry. Unlike other mass spectrometry texts, this comprehensive reference provides systematic descriptions of the various types of mass analysers and ionisation, along with corresponding strategies for interpretation of data. The book concludes with a comprehensive 3000 references. This multi-disciplined text covers the fundamentals as well as recent advance in this topic, providing need-to-know information for researchers in many disciplines including pharmaceutical, environmental and biomedical analysis who are utilizing mass spectrometry

Download or read book Advancing Academics Through Laboratory and Classroom Research written by Lance E. Talbert and published by . This book was released on 2019 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of mass spectrometry (MS) as a tool for the characterization of biological molecules has seen rapid growth over the past three decades. The structure of a peptide or protein is key to its function and the role it plays within a biological environment. Several tools are available for determination of primary structure, however higher order structural characterization continues to be more challenging. As such, the development of new analytical methodology for the structural characterization of peptides and proteins is of significant interest.