Download or read book Mapping the Actin and Actin Binding Proteins Interactions written by Jorge M. Ferrer and published by . This book was released on 2007 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: (cont.) Using imaging and microrheology techniques we characterized the effects of filament length and degree of crosslinking on the structural arrangement and mechanical properties of F-actin networks. We found that the mechanical properties of these networks are length-scale dependent. Also, when probed with active methods, the F-actin networks exhibited strain hardening followed by a gradual softening at forces -30 pN, in good agreement with the single molecule rupture force of 28-73 pN. Thus, with the combination of single molecule and network studies, we can expand the knowledge-base on the regulation and control of the cellular machinery starting from the molecular building blocks.
Download or read book Molecular Interactions of Actin written by C.G. dos Remedios and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Actin is one of the most widespread proteins in eukaryotic cells. This book and its companion (Molecular Interactions of Actin. Actin-Myosin Interaction, Actin-Based Regulation) provide an authoritative and opinionated view of the structure and function of this essential protein. Each section includes an historical perspective and a detailed commentary on actin protein chemistry, molecular and cell biology of actin. While some chapters review the body of knowledge of the subject, others contain new experimental data. This book will appeal to research scientists seeking contemporary overviews of actin and its binding proteins. Contributors include senior scientists as well as the new breed of younger scientists.
Download or read book Actin Monomer Binding Proteins written by Pekka Lappalainen and published by Springer Science & Business Media. This book was released on 2008-05-14 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive view on actin monomer-binding proteins and the mechanisms by which they contribute to actin dynamics and various actin-dependent cellular processes. This new title contains chapters that describe the basic mechanisms of actin dynamics as well as the structural principles by which various actin-binding proteins interact with actin. The book is suitable further reading for students in advanced cell biology classes.
Download or read book Molecular Interactions of Actin written by D.D. Thomas and published by Springer Science & Business Media. This book was released on 2012-11-06 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: Actin is one of the most widespread proteins in eukaryotic cells. This book and its companion ("Molecular Interactions of Actin. Actin Structure and Actin-Binding Proteins") provide an authoritative and opinionated view of the structure and function of this essential protein. Each section includes an historical perspective and a detailed commentary on actin protein chemistry, molecular and cell biology of actin. While some chapters review the body of knowledge of the subject, others contain new experimental data. This book will appeal to research scientists seeking contemporary overviews of actin-myosin interaction and actin-based regulation. Contributors include senior scientists as well as the new breed of younger scientists.
Download or read book Role of the 18 29 Amino Acid Sequence in Actin Function and Mapping of Caldesmon Binding Sites on Actin written by Susan Braver Adams and published by . This book was released on 1994 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mapping the Interaction of Cofilin with Actin written by Sabrina Amelle Benchaar and published by . This book was released on 2007 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mapping the Actin binding Protein Molecule Using Monoclonal Antibodies written by Sheila Egan Tucker and published by . This book was released on 1988 with total page 87 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mapping Functional Interactions on the Surface of Actin written by Jacqueline A. Hendries and published by . This book was released on 2004 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Cytoskeleton written by James Spudich and published by . This book was released on 1996 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Interaction of MAPs and Kinesin Motors with the Neuronal Cytoskeleton written by Jawdat Al-Bassam and published by . This book was released on 2003 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of neuronal architecture requires the microtubule and f-actin cytoskeleton. In developing neurons, the organization and dynamics of these cytoskeletal polymers are regulated by a diverse group of microtubule and f-actin associated proteins. However, microtubules and f-actin remain essential for the maintenance of neuronal architecture, as they are utilized as tracks by motor proteins for the transport a diverse organelles and macromolecules to and from the axons and dendrites. In this work, we investigate the conformations and biochemical activities of these two general classes of proteins. In chapter I, we show that although MAP2 and tau are unstructured in solution, they bind and fold onto the outer ridges of microtubule protofilaments. The extended MAP2 and tau conformations suggest that they stabilize microtubules by bridging multiple tubulins along protofilaments. Even though MAP2 and tau share homologous microtubule binding domains, we show in chapter II that this region of MAP2, but not the homologous region of tau, bind and bundles f-actin. We show that the MAP2-actin binding interaction is necessary for neurite initiation in cells. Based on this work, we propose that MAP2 may coordinate f-actin with microtubules during the neurite initiation process. In Chapter III, we studied the conformations of Unc104 kinesin; we show that the [alpha]-helices of the Unc104 neck can make two types of interactions: an intramolecular coiled coil in a monomeric motor, or an intermolecular coiled coil in a dimeric motor. Our work suggests the self-folding of the Unc104 neck regulates its dimerization, which is necessary for activation of fast processive motility. In chapter IV, we compared the microtubule binding interactions of Unc104 and conventional kinesin in the weak ADP state. We show that Unc104 and conventional kinesin bind microtubules with different binding modes; the Unc104.ADP binding is primarily mediated by the class conserved K-loop, whereas kinesin.ADP binding is mediated by the motor domain. However, MAP2 acts primarily as a competitive inhibitor of both motors binding to microtubules, in spite of their unique binding modes. A comparison of microtubule bound motor and MAP structures explains these biochemical observations, and suggests that steric hindrance due to the overlap of MAP and motor binding sites is primarily responsible for these inhibition effects. Our work suggests that the binding of MAPs and kinesins to microtubules must be locally regulated to prevent MAPS from interfering with organelle transport mediated by motor proteins. Our work suggests that MAPs and Kinesins utilize different strategies to bind the neuronal cytoskeleton. Furthermore, our work provides a number of examples supporting the notion that the evolution of different MAPs and kinesins has specified their polymer binding modes to their specialized functions in the cell.
Download or read book Mass Spectrometry Analysis for Protein Protein Interactions and Dynamics written by M. Chance and published by John Wiley & Sons. This book was released on 2008-09-22 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins. Preliminary chapters cover mass spectrometry methods for examining proteins and are then followed by chapters devoted to presenting very practical, how-to methods in a detailed way. Includes footprinting and plistex specifically, setting this book apart from the competition.
Download or read book Cell Polarity in Development and Disease written by Douglas W Houston and published by Academic Press. This book was released on 2017-11-14 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Polarity in Development and Disease offers insights into the basic molecular mechanisms of common diseases that arise as a result of a loss of ordered organization and intrinsic polarity. Included are diseases affecting highly polarized epithelial tissues in the lung and kidney, as well as loss and gain of cell polarity in the onset and progression of cancer. This book provides a basic resource for understanding the biology of polarity, offering a starting point for those thinking of targeting cell polarity for translational medical research. Provides basic science understanding of cell polarity disease and development Covers diseases affecting polarized epithelial tissues in the lung and kidney, also covering the progression of cancer Includes historical context of cell polarity research for potential future breakthroughs
Download or read book Effects of Actin Binding Proteins Tropomodulins and Tropomyosins on Dynamics of Actin Filaments in Non muscle Cells written by Balaganesh Kuruba and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability of the brain to adapt to environmental or activity-dependent changes is regarded as synaptic plasticity. Synaptic communication occurs between presynaptic terminal (axon) and post synaptic terminal, which in a neuron is an actin-rich protrusion arising from dendrites called spines. Dendritic spines are highly dynamic structures and the synaptic input dependent regulation of their morphology correlates to structural synaptic plasticity. Systematic modulation of cytoskeletal actin by numerous actin binding and regulating proteins, is essential for spine morphogenesis, maturation, stabilization, and organization. Cytoskeletal actin is composed of monomeric G-actin. This monomeric G-actin polymerizes to form polymeric filamentous actin (F-actin) in various forms such as linear, bundled, branched, mesh, etc. Actin filaments are highly dynamic structures with a slow growing (pointed) end and a fast growing (barbed) end. The kinetics of F-actin depolymerization at the pointed end serves as the rate limiting step for maintaining the cytoplasmic pool of G actin for filament assembly. Regulation of F-actin length and dynamics by the pointed end binding proteins still needs to be fully understood, especially in non-muscle cells. Due to the structural complexities posed by the pointed-end binding proteins - tropomodulin (Tmod) and tropomyosin (Tpm) - there is a lack of 3D structural information which is essential to understand the mechanism of the pointed end regulation. Very few studies have been conducted on the role of pointed-end binding proteins in regulation of dendritic spine morphology.In this dissertation, the work presented focuses on highlighting the role played by tropomodulin 2 (Tmod2), a brain-specific isoform, on the dendritic spine re-organization. Tmod2 regulates actin-polymerization by binding to the F-actin pointed end with its distinct actin and tropomyosin (Tpm) binding sites. To provide better understanding of the pointed end regulation by Tpm and Tmod, we engineered Tpm fragments that were used to obtain structural information regarding Tpm-Tmod interface at the pointed end. We provided the structural basis for this interface using circular dichroism (CD), nuclear magnetic resonance (NMR) spectroscopic techniques and molecular dynamic simulations (MDS) to map the region of Tpm-Tmod interactions. We further investigated the effects of overexpressing Tmod2 on the morphological reorganization of dendritic spines and other actin-based structures, after they were formed. We provided information that illuminated the effects that Tmod2 overexpression had on numbers of thin, mushroom, and stubby spines, and other actin-based structures - branched spines, excitatory shaft synapses, dendritic filopodia and spinules. We also provided data on the effects Tmod2 had on dendritic spine motility. We show that Tpm-binding and actin-binding abilities of Tmod2 have distinct roles in spine reorganization, thus accentuating the role of pointed end regulation in dendritic spine related structural synaptic plasticity.
Download or read book Interactions of Actin Myosin and an Actin binding Protein of Rabbit Pulmonary Macrophages III written by John Henry Hartwig and published by . This book was released on 1976 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA protein Interactions written by Kiyoshi Nagai and published by Oxford University Press, USA. This book was released on 1994 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.
Download or read book Actin written by Peter Sheterline and published by OUP Oxford. This book was released on 1999-01-28 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: Actins are a highly conserved family of proteins found in virtually all eukaryotic cells. They have prolific roles in cell motility - from the contraction of striated muscle to the movement of organelles within cells, and are known to interact with a diverse number of proteins families from myosins to gelsolins. This up-to-date edition gives a comprehensive account of actin sequence, mutation and structure as well as providing insight into ligand-binding sites and drug and toxin binding. Illustrated throughout, this modern text also contains an extensive bibliography for the interested reader.
