Download or read book Mammalian Alkaline Phosphatases written by José Luis Millan and published by John Wiley & Sons. This book was released on 2006-12-13 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review and discussion of new knowledge on the structure and function of mammalian alkaline phosphatases (APs) gained over the last 25 years. It covers: * The structure, regulation and expression of the AP genes * The three-dimensional structure of APs and mutagenesis work that further defined the structural/functional domains of the isozymes * The phenotypic abnormalities of the different AP knockout mice * Our current understanding of the in vivo role of the AP isozymes. The book also describes the possible use of APs as therapeutic agents and therapeutic targets and the many uses of these enzymes in clinical medicine and in biotechnology.
Download or read book Mammalian Alkaline Phosphatases written by José Luis Millán and published by Wiley-Blackwell. This book was released on 2006-02-28 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review and discussion of new knowledge on the structure and function of mammalian alkaline phosphatases (APs) gained over the last 25 years. It covers: * The structure, regulation and expression of the AP genes * The three-dimensional structure of APs and mutagenesis work that further defined the structural/functional domains of the isozymes * The phenotypic abnormalities of the different AP knockout mice * Our current understanding of the in vivo role of the AP isozymes. The book also describes the possible use of APs as therapeutic agents and therapeutic targets and the many uses of these enzymes in clinical medicine and in biotechnology.
Download or read book Alkaline Phosphatase written by Robert B. McComb and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 998 pages. Available in PDF, EPUB and Kindle. Book excerpt: There can be no doubt that alkaline phosphatase is one of the most extensively in vestigated of all enzymes. This has resulted from the ubiquity of its distribution, and from the ease and sensitivity with which its activity can be measured. Unfortunately, these wide-ranging but often superficial experimental studies have been followed up by intensive and systematic investigations in only a few limited areas of the biochemistry and chemical pathology of alkaline phosphatase. The result has been the accumulation of a scientific literature of intimidating proportions, and the inevitable rediscovery of already known facts about the enzyme. Scientists are taught early in their careers that, in the words of Sir John Herschel, "Hasty generalization is the bane of science. " Nevertheless, moments arrive in all spheres of scientific activity when generalization becomes essential, to codify and to select from the mass of data already accumulated, and to provide starting points for new developments and new lines of investigation. This is especially true in a field such as alkaline phosphatase research, in which very real dangers exist that the seeds of fundamental understanding will be lost amidst an unexamined harvest of empirical observations. The history of the study of alkaline phosphatase provides several instances when valuable generalizations have emerged. Occasionally, the conclusions drawn on the basis of available evidence were wrong; more frequently, they have stood the test of further experimentation, and always, they have provided new insights into the nature and proper ties of this enzyme.
Download or read book Phosphatase Modulators written by José Luis Millán and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driving further the research on mammalian alkaline phosphatase structure and function, Phosphatase Modulators collects expert contributions into one “how to” manual for basic scientists interested in initiating a drug discovery effort. While this book contains the traditional method chapters and some typical reviews on the structure and known functions of phosphatases, other contributions are meant to discuss approaches and alternatives useful in making “go/no-go” decisions in high throughput screening (HTS) and lead optimization campaigns. Many chapters focus on tissue-nonspecific alkaline phosphatase (TNAP) as well as protein phosphatases. Written for the highly successful Methods in Molecular Biology series, chapters in this volume include the kind of detail and key implementation advice that promotes reproducible results. Step-by-step and practical, Phosphatase Modulators offers a path to understanding many of the facets and complexities associated with undertaking a drug discovery effort and will serve as a roadmap to initiating those efforts.
Download or read book Purification and Properties of Human Alkaline Phosphatases written by Lorne Edwin Seargeant and published by . This book was released on 1979 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A general procedure for the purification of alkaline phosphatase from human tissues using affinity chromatography on a phosphonic acid- Sepharose derivative was developed. The enzymes from human liver, kidney, intestine, placenta and the serum of a patient with Paget's disease of bone were purified to apparent homogeneity as judged by several criteria. Some catalytic properties of alkaline phosphatase purified from human liver were studied. Under in vitro conditions the enzyme catalysed the hydrolysis of a number of phosphomonoesters which have been suggested to be possible physiological substrates. The V values for the hydrolysis of most substrates were very similar and the lowest K values were obtained at near-neutral pH. A calcium-sensitive ATPase activity could not be demonstrated, even in the presence of a calcium-dependent regulator protein. Ca and Mg ions abolished the ATPase and pyrophosphatase activities of liver alkaline phosphatase by formation of metal-substrate complexes. Phosphodiesters and phosphonic acids were not substrates of the enzyme although the latter compounds were inhibitors. The inhibition of liver alkaline phosphatase by phosphate (K^ = 35 yM) suggested that the enzyme activity in vivo may be largely inhibited. Vanadate was a potent competitive inhibitor of alkaline phosphatases from several sources (K_^ values were less than 1 yM) . The inhibition at physiological concentrations of vanadate, and the reversal of this inhibition by compounds such as L-epinephrine indicated that alkaline phosphatase may be regulated by vanadate. The removal of sialic acid residues from liver alkaline phosphatase by neuraminidase treatment caused the isoelectric point to change from 4.0 to 6.5, but had no influence on the specific activity of the enzyme, the values for six substrates, or the inhibition by L-homoarginine. The neuraminidase-treated enzyme was inactivated by heating at 56°C at the same rate as the native enzyme, but was inactivated by SDS more rapidly than the native enzyme. The amino acid compositions from 13 reports of alkaline phosphatase from mammalian and bacterial sources were compared. With the exception of the enzyme from human intestine, the results suggested that a high degree of compositional similarity was present. Apparent subunit molecular weight values, determined by SDS-PAGE for human alkaline phosphatases from liver, kidney, intestine, and Paget's serum were similar (92000-96000). The corresponding value for the enzyme from placenta w T as 74000. Maps of the radioiodinated peptides of alkaline phosphatase purified from human liver, kidney and Paget's serum were highly similar whereas the corresponding maps of the enzyme purified from intestine or placenta were different from each other and from the maps for the enzymes from the other tissues. These results and the data from heat inactivation and differential inhibition studies strongly suggest that three different structural genes code for the protein moiety of the alkaline phosphatase present in these tissues. The general purification protocol and the procedure for mapping the tryptic peptides from alkaline phosphatase radioiodinated within polyacrylamide gels appear to be useful for the characterization and structural identification of tumor-associated alkaline phosphatases.
Download or read book Human Alkaline Phosphatases written by Torgny Stigbrand and published by . This book was released on 1984 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Phosphoric Acid Industry written by Michael Schorr and published by BoD – Books on Demand. This book was released on 2017-07-12 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phosphoric acid is an important industrial acid that is utilized for manufacturing phosphatic fertilizers and industrial products, for pickling and posterior treatment of steel surfaces to prevent corrosion, for ensuring appropriate paint adhesion, and for the food and beverages industry, e.g., cola-type drinks to impart taste and slight acidity and to avoid iron sedimentation. This industry is spread out in countries of four continents - Asia, Africa, America, and Europe - which operate mines and production plants and produce fertilizers. Phosacid is one of the most widely known acids. The global phosacid market and its many phosphate derivatives are expanding worldwide; this trend is expected to continue in the next years, thus producing innovative products.
Download or read book Purification and Properties of Alkaline Phosphatase from Bovine Mammary Tissue written by Richard Brendan O'Keeffe and published by . This book was released on 1978 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Alkaline Phosphatases written by Torgny Stigbrand and published by John Wiley & Sons. This book was released on 1984-11 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Therapeutic Enzymes Function and Clinical Implications written by Nikolaos Labrou and published by Springer Nature. This book was released on 2019-09-03 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Therapeutic enzymes exhibit fascinating features and opportunities, and represent a significant and promising subcategory of modern biopharmaceuticals for the treatment of several severe diseases. Research and drug developments efforts and the advancements in biotechnology over the past twenty years have greatly assisted the introduction of efficient and safe enzyme-based therapies for a range of both rare and common disorders. The introduction and regulatory approval of twenty different recombinant enzymes has enabled effective enzyme-replacement therapy. This volume aims to overview these therapeutic enzymes, focusing in particular on more recently approved enzymes produced by recombinant DNA technology. This volume is composed of four sections. Section 1 provides an overview of the production process and biochemical characterization of therapeutic enzymes, while Section 2 focuses upon the engineering strategies and delivery methods of therapeutic enzymes. Section 3 highlights the clinical applications of approved therapeutic enzymes, including aspects on their structure, indications and mechanisms of action. Together with information on these mechanisms, safety and immunogenicity issues and various adverse events of the recombinant enzymes used for therapy are discussed. Section 4, provides discussion on the prospective and future developments of new therapeutic enzymes. This book is aimed at academics, researchers and students undertaking advanced undergraduate/postgraduate programs in the biopharmaceutical/biotechnology area who wish to gain a comprehensive understanding of enzyme-based therapeutic molecules.
Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Download or read book Phosphatase Modulators written by José Luis Millán and published by Humana Press. This book was released on 2013-07-17 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driving further the research on mammalian alkaline phosphatase structure and function, Phosphatase Modulators collects expert contributions into one “how to” manual for basic scientists interested in initiating a drug discovery effort. While this book contains the traditional method chapters and some typical reviews on the structure and known functions of phosphatases, other contributions are meant to discuss approaches and alternatives useful in making “go/no-go” decisions in high throughput screening (HTS) and lead optimization campaigns. Many chapters focus on tissue-nonspecific alkaline phosphatase (TNAP) as well as protein phosphatases. Written for the highly successful Methods in Molecular Biology series, chapters in this volume include the kind of detail and key implementation advice that promotes reproducible results. Step-by-step and practical, Phosphatase Modulators offers a path to understanding many of the facets and complexities associated with undertaking a drug discovery effort and will serve as a roadmap to initiating those efforts.
Download or read book Phosphate Metabolism written by Shaul Massry and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 611 pages. Available in PDF, EPUB and Kindle. Book excerpt: We present to our readers the proceedings of the Second International Workshop on Phosphate. A short account of the history of the effort led to the Phosphate Workshops is appro priate and can be of interest to the reader. The idea for Phosphate Workshops was born in the early days of November, 1974. One of us (S. G. M. ) suggested the thought to a group of scientists gathered for a luncheon in one of the attrac tive small restaurants in Weisbaden, Germany. The purpose of the workshop was to bring together interested scientists to discuss the newer developments and the recent advances in the field of phosphate metabolism and the other related minerals. An Organizing Committee made of Shaul G. Massry (USA), Louis V. Avioli (USA), Philippe Bordier (France), Herbert Fleisch (Switzerland), and Eduardo Slatopolsky (USA) was formed. The First Workshop was held in Paris during June 5-6, 1975 and was hosted by Dr. Philippe Bordier. Its proceeding was already published. The Second Workshop took place in Heidelberg during June 28-30, 1976 and was hosted by Dr. Eberhard Ritz. Both of these workshops were extremely successful scientific endeavors, and the need for them was demonstrated by the great interest they generated among the scientific community. The Or ganizing Committee, therefore, decided to continue with the tradi tion to hold additional Workshops annually or every other year.
Download or read book How Tobacco Smoke Causes Disease written by and published by . This book was released on 2010 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report considers the biological and behavioral mechanisms that may underlie the pathogenicity of tobacco smoke. Many Surgeon General's reports have considered research findings on mechanisms in assessing the biological plausibility of associations observed in epidemiologic studies. Mechanisms of disease are important because they may provide plausibility, which is one of the guideline criteria for assessing evidence on causation. This report specifically reviews the evidence on the potential mechanisms by which smoking causes diseases and considers whether a mechanism is likely to be operative in the production of human disease by tobacco smoke. This evidence is relevant to understanding how smoking causes disease, to identifying those who may be particularly susceptible, and to assessing the potential risks of tobacco products.
Download or read book Enzyme Synthesis and Degradation in Mammalian Systems written by Miloslav Rechcígl and published by . This book was released on 1971 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applied Manure and Nutrient Chemistry for Sustainable Agriculture and Environment written by Zhongqi He and published by Springer Science & Business Media. This book was released on 2014-04-07 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the rapid increase in world population and improving living standards, the global agriculture sector is confronting with challenges for the sustainability of agricultural production and of the environment. Intensive high-yield agriculture is typically dependent on addition of fertilizers (synthetic chemicals, animal manure, etc.). However, non-point nutrient losses from agricultural fields due to fertilization could adversely impact the environment. Increased knowledge on plant nutrient chemistry is required for improving utilization efficiency and minimizing loses from both inorganic and organic nutrient sources. For this purpose, the book is composed of 19 chapters that highlight recent research activities in applied nutrient chemistry geared toward sustainable agriculture and environment. Topics of interest include, but are not limited, to speciation, quantification, and interactions of various plant nutrients and relevant contributories in manure, soil, and plants. This book outlooks emerging researchable issues on alternative utilization and environmental monitoring of manure and other agricultural by products that may stimulate new research ideas and direction in the relevant fields.
Download or read book Purinergic Signaling written by Pablo Pelegrín and published by Humana. This book was released on 2020-11-07 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.
