Download or read book Lysyl tRNA Synthetases of E Coli written by Morad Hassani and published by . This book was released on 1995 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Characterization of a Lysyl tRNA Synthetase Mutant of E Coli K 12 written by Ramon Tenreiro and published by . This book was released on 1982 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mapping and Characterization of the Constitutive Lysyl tRNA Synthetase Gene of Escherichia Coli K 12 written by Rosemarie V. Emmerich and published by . This book was released on 1987 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Engineering of Lysyl TRNA Synthetase LysU from Escherichia Coli written by Alison Damaris Hindley and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Comparative Study of Low PH Stress in E Coli and S Typhimurium and a Comparative Study of the Inducibility of Lysyl tRNA Synthetase in the Enterobacteriaceae written by Edward William Hickey and published by . This book was released on 1988 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book ThioA written by Rodger P. Silletti and published by . This book was released on 1985 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Aminoacyl tRNA Synthetases written by Michael Ibba and published by CRC Press. This book was released on 2005-04-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: By virtue of their role as catalysts of the aminoacylation reaction, the aminoacyl-tRNA synthetases ensure that the first step of translation is performed quickly and accurately. In this volume of 36 separate chapters, the many facets of this ancient and ubiquitous family are reviewed, including their surprising structural diversity, enzymology, tRNA interaction properties, and curious alternative functions. These chapters illustrate the degree to which the aminoacyl-tRNA synthetases employ a variety of mechanisms to carry out both the standard functions related to the synthesis of aminoacylated tRNA for protein synthesis, as well as the surprising functions associated with amino acid biosynthesis, cytokine function, and even the processivity of DNA replication. Other chapters explore the regulation of their synthesis, their role in disease, and their prospects as targets for antibacterial therapeutics. This monograph will be a valuable resource for all scientists interested in the fundamentals of protein synthesis from both a basic research and clinical perspective, as well as the relation of translational components to the evolution of the genetic code.

Download or read book Mutational Alterations in the Aminoacyl TRNA Synthetase System of Escherichia Coli written by William Robert Folk and published by . This book was released on 1970 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Non Natural Amino Acids written by and published by Academic Press. This book was released on 2009-07-24 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. - Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube - Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules - Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Download or read book Rationale for the Evolutionary Retention of Two Unrelated Lysyl tRNA Synthetases written by Sandro Fernandes Ataide and published by . This book was released on 2006 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Lysine insertion during coded protein synthesis requires lysyl-tRNALys, which is synthesized by lysyl-tRNA synthetase (LysRS). Two unrelated forms of LysRS are known: LysRS2, which is found in eukaryotes, most bacteria and a few archaea, and LysRS1, which is found in most archaea and a few bacteria. A detailed comparison of the amino acid recognition strategies of LysRS1 (Borrelia burgdorferi) and LysRS2 (Escherichia coli) was undertaken by studying the effects of lysine analogues on the aminoacylation reaction in vitro and in vivo. Also, based on comparisons of crystal structures and discrimination of lysine analogues by both LysRSs, the roles of the key residues in the active site of LysRS2 (lysS encoded) from E. coli were determined in vitro and in vivo. The differences in resistance to naturally occurring non-cognate amino acids suggest the distribution of LysRS1 and LysRS2 contributes to quality control during protein synthesis. LysRS1 and LysRS2 are not normally found together within one organism. In the pathogen Bacillus cereus both forms of LysRS are encoded; to investigate what role these two LysRSs might play in B. cereus, their RNA substrate specificities were investigated. It was found that in B. cereus the two different LysRSs together aminoacylate a small RNA of unknown function named tRNAOther. Aminoacylation of tRNAOther was found to be confined to stationary phase, suggesting a role for this non-canonical tRNA in growth phase-specific protein synthesis. Analysis of the non-canonical Watson-Crick base pairs and a bulge in the predicted secondary structure of tRNAOther indicate the importance of these identity elements in recognition by the LysRS1:LysRS2 complex. The role of tRNAOther in B. cereus was also investigated by the construction of a deletion strain, indicating that tRNAOther is not an essential gene. The results implicate tRNAOther in multiple regulatory functions that remain, as yet, uncharacterized.

Download or read book The Occurrence of Duplicate Lysyl tRNA Dunthetase Genes in E Coli and Other Procaryotes written by Maria V. Saluta and published by . This book was released on 1992 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studies on Aminoayl TRNA Synthetases from Escherichia Coli written by Wayne N. Marchant and published by . This book was released on 1973 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Genetics written by Robert C. King and published by Springer. This book was released on 1974-11-01 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this and future volumes of the Handbook of Genetics is to bring together a collection of relatively short, authoritative essays or annotated compilations of data on topics of significance to geneticists. Many of the essays will deal with various aspects of the biology of certain species selected because they are favorite subjects for genetic investigation in nature or the laboratory. Often there will be an encyclopedic amount of information available on such a species, with new papers appearing daily. Most of these will be written for specialists in a jargon that is be wildering to a novice, and sometimes even to a veteran geneticist working with evolutionarily distant organisms. For such readers what is needed is a written introduction to the morphology, life cycle, reproductive be havior, and culture methods for the species in question. What are its par ticular advantages (and disadvantages) for genetic study, and what have we learned from it? Where are the classic papers, the key bibliographies, and how does one get stocks of wild type or mutant strains? The chapters devoted to different species will contain information of this sort. Only a few hundreds of the millions of species available to biologists have been subjected to detailed genetic study. However, those that have make up a very heterogeneous sample of the living world.

Download or read book A Study to Investigate the Coordinate Expression of TRNA Synthetases in E Coli written by Susan Lorraine Tausta and published by . This book was released on 1984 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book TRNA written by Dieter Söll and published by . This book was released on 1995 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Orthogonality and Codon Preference of the Pyrrolysyl tRNA Synthetase tRNAPyl Pair in Escherichia Coli for the Genetic Code Expansion written by Keturah Odoi and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Systematic studies of basal nonsense suppression, orthogonality of tRNAPyl variants, and cross recognition between codons and tRNA anticodons are reported. E. coli displays detectable basal amber and opal suppression but shows a negligible ochre suppression. Although detectable, basal amber suppression is fully inhibited when a pyrrolysyl-tRNA synthetase (PylRS)-tRNAPyl_CUA pair is genetically encoded. trnaPyl_CUA is aminoacylated by an E. coli aminoacyl-tRNA synthetase at a low level, however, this misaminoacylation is fully inhibited when both PylRS and its substrate are present. Besides that it is fully orthogonal in E. coli and can be coupled with PylRS to genetically incorporate a NAA at an ochre codon, tRNAPyl_UUA is not able to recognize an UAG codon to induce amber suppression. This observation is in direct conflict with the wobble base pair hypothesis and enables using an evolved M. jannaschii tyrosyl-tRNA synthetase-tRNAPyl_UUA pair and the wild type or evolved PylRS-tRNAPyl_UUA pair to genetically incorporate two different NAAs at amber and ochre codons. tRNAPyl_UCA is charged by E. coli tryptophanyl-tRNA synthetase, thus not orthogonal in E. coli. Mutagenic studies of trnaPyl_UCA led to the discovery of its G73U form which shows a higher orthogonality. Mutating trnaPyl_CUA to trnaPyl_UCCU not only leads to the loss of the relative orthogonality of tRNAPyl in E. coli but also abolishes its aminoacylation by PylRS.

Download or read book Ap4a and Other Dinucleoside Polyphosphates written by Alexander G. McLennan and published by CRC Press. This book was released on 1992-05-22 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ap4A and Other Dinucleoside Polyphosphates is the first compilation of contributions from major investigators involved in studying the chemistry and biology of dinucleoside polyphosphates. The book features reviews and original research (including unpublished and anecdotal material) covering the whole area of dinucleoside polyphosphate research from its inception to the present day. Ap4A and Other Dinucleoside Polyphosphates will be a useful reference for biological scientists studying nucleotide metabolism, cell signalling, DNA replication and repair, and cellular response to stress.