Download or read book Luminescence Spectroscopy and Imaging written by L. Nasdala and published by . This book was released on 2013 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescence Spectroscopy Imaging and Probes written by Ruud Kraayenhof and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.
Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.
Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Download or read book Topics in Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.
Download or read book Topics in Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy continues its advance to more sophisticated methods and applications. As one looks over the previous decades, its appears that the first practical instruments for time-resolved measurements appeared in the 1970’s. The instrumentation and analysis methods for time-resolved fluorescence advanced rapidly throughout the 1980’s. Since 1990 we have witnessed a rapid migration of the principles of time-resolved fluorescence to cell biology and clinical appli- tions. Most recently, we have seen the introduction of multi-photon excitation, pump-probe and stimulated emission methods for studies of biological mac- molecules and for cellular imaging. These advanced topics are the subject of the present volume. Two-photon excitation was first predicted by Maria Goppert-Mayer in 1931, but was not experimentally observed until 1961. Observation of two-photon excitation required the introduction of lasers which provided adequate photon density for multi-photon absorption. Since the early observations of two-photon excitation in the 1960s, multi-photon spectroscopy has been limited to somewhat exotic applications of chemical physics, where it is used to study the electronic symmetry of small molecules. Placing one’s self back in 1980, it would be hard to imagine the use of multi-photon excitation in biophysics or cellular imaging.
Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by . This book was released on 2014-01-01 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.
Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
Download or read book Advances in Luminescence Spectroscopy written by L. J. Cline Love and published by ASTM International. This book was released on 1985 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optical Spectroscopy And Imaging For Cancer Diagnostics Fundamentals Progress And Challenges written by Noureddine Melikechi and published by World Scientific. This book was released on 2023-01-06 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an interdisciplinary book that presents the applications of novel laser spectroscopy and imaging techniques for the detection of cancers recently developed by some of the world's most renown researchers. The book consists of three parts and a total of 16 chapters. Each chapter is written by leading experts who are actively seeking to develop novel spectroscopic and analytical methods for cancer detection and diagnosis.In Part I, the authors present fundamentals on optics, atoms and molecules, biophysics, cancer and machine learning. These chapters are intended for those who are not experts in the field but wish to learn about fundamentals' aspects of some of the key topics that are addressed in this book. Particular attention has been given to providing key references for those who wish to go further into the fundamental aspects of atoms and molecules, light-matter interaction, optical instrumentation, machine learning and cancer.In Part II, the authors present key applications of various laser spectroscopic methods in cancer diagnosis. They have provided recent progress in cancer diagnostics obtained by combining laser spectroscopy and machine learning for the analysis of the spectra acquired from biomedical tissues and biofluids.In Part III, the authors present chapters that discuss key developments in the applications of various laser imaging techniques for cancer detection.This is one of the few books that addresses cancer detection and diagnosis using laser spectroscopic and imaging tools with an eye on providing the reader the scientific tools, including machine learning ones.
Download or read book Topics in Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial two volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. The third volume will appear shortly. The first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).
Download or read book New Trends in Fluorescence Spectroscopy written by Bernard Valeur and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Download or read book Luminescence written by Jagannathan Thirumalai and published by BoD – Books on Demand. This book was released on 2016-11-10 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this book is to give readers a broad review of topical worldwide advancements in theoretical and experimental facts, instrumentation and practical applications erudite by luminescent materials and their prospects in dealing with different types of luminescence like photoluminescence, electroluminescence, thermo-luminescence, triboluminescence, bioluminescence design and applications. The additional part of this book deals with the dynamics, rare-earth ions, photon down-/up-converting materials, luminescence dating, lifetime, bioluminescence microscopical perspectives and prospects towards the basic research or for more advanced applications. This book is divided into four main sections: luminescent materials and their associated phenomena; photo-physical properties and their emerging applications; thermoluminescence dating: from theory to applications, and bioluminescence perspectives and prospects. Individual chapters should serve the broad spectrum of common readers of diverse expertise, layman, students and researchers, who may in this book find easily elucidated fundamentals as well as progressive principles of specific subjects associated with these phenomena. This book was created by 14 contributions from experts in different fields of luminescence and technology from over 20 research institutes worldwide.
Download or read book Proceedings of Physiological Imaging Spectroscopy and Early detection Diagnostic Methods written by Randall L. Barbour and published by . This book was released on 1993 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Modern Luminescence Spectroscopy of Minerals and Materials written by Michael Gaft and published by Springer. This book was released on 2015-11-29 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is devoted to three types of laser-based spectroscopy of minerals, namely Laser-Induced Time-Resolved Luminescence, Laser-Induced Breakdown spectroscopy and Gated Raman Spectroscopy. This new edition presents the main new data, which have been received after the publication of the first edition ten years ago both by the authors and by other researchers. During this time, only the authors published more than 50 original papers devoted to laser-based spectroscopy of minerals. A lot of new data have been accumulated, both in fundamental and applied aspects, which are presented in new edition.
Download or read book Single Molecule Fluorescence Spectroscopy and Imaging Advanced Methods and Applications in Life Sciences written by Arindam Ghosh and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The visualization of biological structures down to the molecular length scale has been recently made possible by the development of super-resolution fluorescence microscopy. These techniques now routinely resolve biological structures down to a few nanometers. Various super-resolution techniques have been developed, the most successful being Stimulated Depletion Emission (STED) microscopy and Single Molecule Localization Microscopy (SMLM). In what follows, I will focus on the latter class of techniques which is based on the fact that a single molecule image allows for localizing the molecul...
Download or read book Fluorescence Methods and Applications written by Otto S. Wolfbeis and published by Wiley-Blackwell. This book was released on 2008-07 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume features papers on new spectroscopic methods and techniques, the development and application of fluorescent probes, and new techniques and applications of fluorescence imaging. Specific areas include the following: fluorescence lifetime, fluorescence (in vivo) imaging, time-resolved fluorescence, luminescence anisotropy, fluorescent (NMIR) labels, luminescent lanthanides, fluorescent sensors and probes, fluorescence microscopy, FRET, fluorescent nanoparticles and dots, high-throughput screening, fluorescent bioassays, luminescence-based DNA technologies, FISH and immunohistochemistry, luminescence on metal surfaces, fluorescent proteins, upconversion, multiphoton fluorescence, confocal techniques, near-field and far-field techniques, single photon counting, fluorescence correlation spectroscopy (FCS), and flow cytometry. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit www.nyas.org/membership/main.asp for more information about becoming a member.
