Download or read book Low Frequency Resonance Raman Studies of Heme Protein Model Complexes written by Melody Lee Mitchell and published by . This book was released on 1984 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Reactive Intermediates and Structure Determination of Heme Proteins Studied by Using Resosnance i e Resonance Raman Spectroscopy written by Jose F. Cerda and published by . This book was released on 2002 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resonance Raman Studies of Heme Complexes and Proteins written by Sunhee Choi and published by . This book was released on 1982 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resonance Raman Studies of Isotopically Labeled Heme Proteins written by Freeborn Rwere and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: One effective approach for exploring structure/function relationships in heme proteins is to study proteins that have been reconstituted with modified hemes so as to systematically perturb the protein-heme interface. However, some reconstituted heme proteins may contain substantial fractions of a "non native" state in which the orientation of the heme in the folded pocket differs from the native conformation by a 180° rotation about the [alph alpha - gamma gamma] meso axis. In fact, this "non native" state has also been shown to exist in some native proteins, including several mammalian globins. In order to define changes in the active site structure associated with this "disorder", we have applied resonance Raman spectroscopy to the metMb derivatives, using selectively deuterated protohemes to associate the observed modes with specific fragments of the heme. Resonance Raman spectroscopy is also employed to characterize heme site structural changes arising from conformational heterogeneity in deoxyMb and ligated derivatives; i.e., the ferrous CO (MbCO) and ferric cyanide (MbCN) complexes. Interestingly, while substantial changes in the disposition of the peripheral vinyl and propionate groups can be inferred from the dramatic spectral shifts, the bonds to the internal histidyl imidazole ligand and those of the Fe-CO and Fe-CN fragments are not significantly affected by the heme rotation, as judged by lack of significant shifts in the [upsilon](Fe-NHis), [upsilon](Fe-C) and [upsilon](C-O) modes. We have synthesized protohemes with selectively labeled vinyl groups and have effectively reconstituted them into apo-myoglobin in order to assign the so-called "vinyl bending" modes of heme group in native and reversed forms of myoglobin to their specific molecular fragments based on their isotopic shift with these vinyl labeled protohemes. In a separate project, these vinyl labeled hemes have been employed to further define structural changes in cytochrome P450cam upon substrate binding. Substrate binding to cytochrome P450cam is known to induce the distortions of the out of plane modes such as [gamma gamma]6 and [gamma gamma]7 modes as well as the heme peripheral substituents. The detection of these low frequency modes is especially important as the disposition of these groups can modify the heme reduction potential.

Download or read book The Application of Optical Absorption and Resonance Raman Spectroscopy to the Study of Heme Proteins and Model Compounds written by Robert T. Kean and published by . This book was released on 1987 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resonance Raman Studies of Proteins and Copper Protein Model Complexes written by Debra Sue Caswell and published by . This book was released on 1986 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resonance Raman Studies of Hemoproteins and Model Heme Complexes written by Shun-Hua Lin and published by . This book was released on 1987 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resonance Raman Spectra of Heme and Metalloproteins written by Thomas G. Spiro and published by Wiley-Interscience. This book was released on 1988 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resonance Raman Spectroscopy as a Probe of Heme Protein Structure and Dynamics written by Thomas G. Spiro and published by . This book was released on 1985 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Resonance Raman Study of Ligand Binding to Model Heme Complexes and Hemoproteins written by Ellen Augustine Kerr and published by . This book was released on 1984 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resonance Raman Studies of Protein cofactor Interactions in Wild Type and Mutant Purple Bacterial Reaction Centers written by Lei Chen and published by . This book was released on 2005 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Vibrational Spectroscopy in Protein Research written by Yukihiro Ozaki and published by Academic Press. This book was released on 2020-05-19 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies. Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies. Provides thorough guidance in implementing cutting-edge vibrational spectroscopic methods from international leaders in the field Emphasizes in vivo, in situ and non-invasive analysis of proteins in biomedical and life science research more broadly Contains chapters that address vibrational spectroscopy for the study of simple purified proteins and protein aggregates, fibrous proteins, membrane proteins and protein assemblies

Download or read book Resonance Raman Studies of Heme Proteins written by Guangzhi Yuan and published by . This book was released on 2004 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resonance and Variable Temperature Raman Studies of Chloroperoxidase and Methemoglobin written by Ronald David Remba and published by . This book was released on 1980 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dissertation Abstracts International written by and published by . This book was released on 2003 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Porphyrins V3 written by David Dolphin and published by Elsevier. This book was released on 2012-12-02 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Porphyrins, Volume III: Physical Chemistry, Part A deals with the physical chemistry of porphyrins, their precursors, catabolic derivatives, and related systems. The book covers electronic structure and spectroscopy including circular dichroism and magnetic circular dichroism as well as electronic, infrared, resonance Raman, and Zeeman spectroscopy. Porphyrin stereochemistry, X-ray crystallography, and mass spectroscopy are also included. This volume is organized into 12 chapters and begins with an overview of the optical absorption and emission spectra of porphyrins and the theory by which these facts are understood. The discussion then shifts to the electronic states of the iron porphyrin complex in various heme proteins as displayed by the light absorption properties in the visible region of the electromagnetic spectrum. The reader is methodically introduced to the electronic absorption spectra and molecular orbital theory of chlorophylls, the magnetic optical activity of porphyrins and hemoproteins, and circular dichroism studies of hemoproteins. The book also examines the mass spectra and infrared spectroscopy of porphyrins, resonance Raman scattering from metalloporphyrins and hemoproteins, and photographic essay of porphyrins and related macrocycles. It then concludes with a chapter on high-resolution Zeeman spectroscopy of metalloporphyrins. This book should be useful to inorganic, organic, physical, and biochemists interested in the physical chemistry of porphyrins.