Download or read book Live Imaging In Zebrafish Insights Into Development And Disease written by Karuna Sampath and published by World Scientific. This book was released on 2010-08-30 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: The zebrafish embryo has now been well established as an organism of choice for the genetic and cell biological basis of vertebrate development. Large-scale forward genetic screens together with reverse genetic tools have resulted in a flood of new information on the regulation of vertebrate development. Furthermore, the unique transparency of the zebrafish embryo allows unprecedented access to cells and tissues and many important discoveries on gene function have already been made using live imaging techniques in this organism. This book successfully brings together state-of-the-art methodologies of live imaging, such as the use of fluorescence correlation spectroscopy (FCS), that will be useful for investigators in the field of developmental biology to address their favorite problem using cell biological approaches in the zebrafishOne of the key reasons that has made the zebrafish such a popular vertebrate model is its transparency and its potential for high-resolution cell biology and imaging. However, there is no comprehensive and up-to-date guide to the new “live imaging” tools that can now be used in the zebrafish. Furthermore, there are many new microscopy methods that have been adapted for use in zebrafish, and this book can be a good guide even for scientists already familiar with the imaging advantages of this model.
Download or read book The Cytoskeleton written by James Spudich and published by . This book was released on 1996 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Confocal Raman Microscopy written by Jan Toporski and published by Springer. This book was released on 2018-03-01 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition provides a cutting-edge overview of physical, technical and scientific aspects related to the widely used analytical method of confocal Raman microscopy. The book includes expanded background information and adds insights into how confocal Raman microscopy, especially 3D Raman imaging, can be integrated with other methods to produce a variety of correlative microscopy combinations. The benefits are then demonstrated and supported by numerous examples from the fields of materials science, 2D materials, the life sciences, pharmaceutical research and development, as well as the geosciences.
Download or read book Germline Development in the Zebrafish written by Roland Dosch and published by Humana. This book was released on 2022-03-06 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Foodborne Bacterial Pathogens written by Arnaud Bridier and published by Humana. This book was released on 2018-12-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens.
Download or read book Imaging from Cells to Animals In Vivo written by Margarida Barroso and published by CRC Press. This book was released on 2020-12-03 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.
Download or read book Imaging Manipulation and Optogenetics in Zebrafish written by Itia Amandine Favre‐Bulle and published by Springer. This book was released on 2018-08-12 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt: The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.
Download or read book Neutrophil Methods and Protocols written by Mark T. Quinn and published by Springer Science & Business Media. This book was released on 2007-08-02 with total page 1099 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.
Download or read book Make Life Visible written by Yoshiaki Toyama and published by Springer Nature. This book was released on 2019-10-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.
Download or read book The Zebrafish Cellular and Developmental Biology Part B written by and published by Academic Press. This book was released on 2011-04-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.
Download or read book Whole brain Voltage Imaging in Larval Zebrafish Using Light field Microscopy written by Zeguan Wang and published by . This book was released on 2020 with total page 68 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monitoring pan-neuronal electrical activity in awake vertebrates is a yet-to-be-achieved goal in neuroscience that will facilitate our efforts to understand how the brain processes information and mediates behaviors. Here we present Sparse Decomposition Light-Field Microscopy (SDLFM), a light-field microscopy method with improved resolution for volumetric neural activity imaging, and computational and experimental evaluations of its capacity for whole-brain voltage imaging in larval zebrafish. The work in this thesis includes three parts. First, we designed and implemented SDLFM and demonstrated its cellular-resolution ability in larval zebrafish expressing pan-neuronal GCaMP6. Second, we simulated the voltage imaging and the computation processes of SDLFM for zebrafish brains and found that in order to achieve a >0.9 correlation coefficient between the extracted SDLFM signals and the real voltage signals with our current hardware and voltage indicators, the proportion of the neurons randomly labeled with fluorescence in a zebrafish brain must be reduced to below 20%. Finally, we imaged live brains of zebrafish larvae expressing pan-neuronal Voltron525-ST and transient SomArchon at a volume rate of 307 Hz with a volumetric field-of-view of ~700 [mu]mx~400 [mu]mx~300 [mu]m. Although spontaneous spikes were traced in these recordings, we speculate some signals might result from noise and artifacts. In summary, we have developed SDLFM, which allows high-speed whole-brain imaging at cellular resolution in larval zebrafish. New camera sensors with higher quantum efficiency and larger dynamic range, and new voltage indicators with increased brightness and sensitivity are needed to achieve the signal-to-noise ratio required to record the voltage activity from a densely labeled fish brain.
Download or read book Sourcebook of Models for Biomedical Research written by P. Michael Conn and published by Springer Science & Business Media. This book was released on 2008-03-07 with total page 756 pages. Available in PDF, EPUB and Kindle. Book excerpt: The collection of systems represented in this volume is a unique effort to reflect the diversity and utility of models used in biomedicine. That utility is based on the consideration that observations made in particular organisms will provide insight into the workings of other, more complex systems. This volume is therefore a comprehensive and extensive collection of these important medical parallels.
Download or read book Essential Zebrafish Methods Genetics and Genomics written by H. William Detrich III and published by Academic Press. This book was released on 2009-09-05 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade" to both experienced researchers and more junior members in the lab. Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error
Download or read book The Zebrafish Cellular and Developmental Biology Part A Cellular Biology written by and published by Academic Press. This book was released on 2016-06-04 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology, is the latest edition in the Methods in Cell Biology series that looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology. Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies Written by experts in the field Contains cutting-edge material on the topic of developmental biology in zebrafish New two part edition of this important volume
Download or read book Exploring the Microclot driven Pre metastatic Niche written by Juma R. R. Ward and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Zebrafish Cellular and Developmental Biology written by and published by Academic Press. This book was released on 2010-12-24 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls Summarizes the Zebrafish Genome Project
Download or read book The Zebrafish Disease Models and Chemical Screens written by H. William Detrich and published by Academic Press. This book was released on 2011-09-23 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls This volume concentrates on Disease Models and Chemical Screens
