Download or read book Liposomes written by Gerard G.M. D'Souza and published by Springer Nature. This book was released on 2023-02-13 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.
Download or read book Liposome Methods and Protocols written by Subhash C. Basu and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.
Download or read book Liposome Methods And Protocols written by Subhash C. Basu and published by . This book was released on 2009-02-01 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Topics I Physical Properties 1. Preparation, Isolation, And Characterisation Of Liposomes Containing Natural And Synthetic Lipids 2. Preparation And Use Of Liposomes For The Study Of Sphingolipid Segregation In Membrane Model Systems II Liposome Fusion/Modulation 3. Peptide-Induced Fusion Of Liposomes 4. Liposomes: Applications In Protein Lipid Interaction Studies 5. Lipids In Viral Fusion III Application Of Liposomes 6. Liposome-Mediated, Fluorescence-Based Studies Of Sphingolipid Metabolism In Intact Cells 7. Micelles And Liposomes In Metabolic Enzyme And Glycolipid Glycosyl Transferase Assays
Download or read book Liposomes written by Gerard G. M. D'Souza and published by . This book was released on 2016 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry. .
Download or read book Liposome Based Drug Delivery Systems written by Wan-Liang Lu and published by Springer. This book was released on 2021-07-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes the protocols for fabrication of liposomal drug delivery system, and consists of two parts. The first part emphasizes the basic protocols and concepts for fabrication of drug-loaded liposomes. For new investigators, laying the groundwork is the first step. This part focuses on the fabrication details in liposomes formulations, which are the so-called small tricks while usually not disclosed in mostly published research articles. However, these processing details are crucial for the preparation of liposomes. And the second part focuses on the strategies in modified and/or functionalized liposomes to adapt to pathophysiological environment, as well as their application in treating diseases. As new analytical and synthetic technologies become available, and improved understanding of pathophysiology, various functionalized drug liposomes are developing to fit the requirements of different therapeutic purposes, exhibiting a broad range of applications. Accordingly, the objectives of this part are aimed at deeply unearthing the formulation of drug-loaded liposomes, describing the detailed operation of liposomal formulation, and further demonstrating their potential applications. Therefore, this volume shows the features of experimental report or protocol for proving a guide to scientists, research students, and young investigators in pharmaceutical enterprises.
Download or read book Liposomes Part E written by and published by Elsevier. This book was released on 2005-02-28 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Part E is a continuation of previous Methods in Enzymology Liposome volumes A, B, C and D. One of the most highly respected publications in the field of biochemistry since 1955 Frequently consulted, and praised by researchers and reviewers alike Truly an essential publication for anyone in any field of the life sciences
Download or read book Liposomes written by Volkmar Weissig and published by Humana Press. This book was released on 2010-04-29 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that “the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer” [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.
Download or read book Liposome Protocols written by Ashok K. Shukla and published by . This book was released on 1999-04-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Liposomes written by Volkmar Weissig and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane.?Volume 1: Pharmaceutical Nanocarriers focuses on the state-of-the-art aspects of developing liposome-based nanoscale drug delivery techniques, delving into the depths of the emerging field of pharmaceutical and biomedical nanotechnology. As a volume in the highly successful Methods in Molecular BiologyTM series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Liposomes: Methods and Protocols promises to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol.
Download or read book Liposomes in Nanomedicine written by Wiley and published by John Wiley & Sons. This book was released on 2012-12-12 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes are synthetic vesicles consisting of one or more phospholipid bilayers, able to accommodate water- and lipid-soluble molecules. They are used as a delivery system for drugs, genes and vaccines in therapeutics. This collection of reviews and laboratory protocols provides the reader with an excellent introduction to some of the many ways in which liposomes can be labeled and used in both diagnostic and therapeutic applications. Diagnostic uses include attachment of radiolabels to track nanometer-sized liposomes in the body in a quantitative fashion using PET or SPECT imaging. The ability to track the uptake of liposomes in humans and research animals on a whole-body basis provides an excellent tool for developing liposome-based drug delivery agents. The attachment of therapeutic molecules to liposomes also has great promise in cancer therapy. In addition, recent developments in the use of liposomes carrying therapeutic radionuclides for cancer therapy will be reviewed. A laboratory protocol is also provided to illustrate how liposomes are routinely used to transfect (deliver) DNA into cells. This e-book is a curated collection from eLS, WIREs, and Current Protocols on the topic of liposomes in nanomedicine. It is a great introduction to the field for students or interdisciplinary collaborators.
Download or read book Liposome Technology written by Gregory Gregoriadis and published by CRC Press. This book was released on 2016-04-19 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposome Technology, Volume I: Liposome Preparation and Related Techniques, Third Edition, is a thoroughly updated and expanded new edition of a classic text in the field. Including step-by-step technical details, Volume I illustrates numerous methods for liposome preparation and auxiliary techniques necessary for the stabilization and characteriza
Download or read book Liposomes A Practical Approach written by V. P. Torchilin and published by Oxford University Press. This book was released on 2003-06-05 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an up-to-date and unique collection of experimental protocols from an area of pharmaceutical research that is essential for the development of new, highly specific drugs as well as for the exploration of completely new therapeutic approaches to disease treatments.
Download or read book Liposomes for Functional Foods and Nutraceuticals written by Sreerag Gopi and published by CRC Press. This book was released on 2022-06-15 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes have been used primarily for drug delivery, and there has been only been limited development of this technology in the food industry. This volume helps to fill that gap by focusing on the advanced trends and applications of liposomes in the nutraceuticals and functional foods industry. The volume begins by discussing the processes and protocols of formation of liposomes and the structures of liposomes produced by different methods. It then reviews their physico-chemical properties and the science of encapsulation of bioactive compounds using liposomes. It continues with an overview of liposomal methods, protocols, preparation techniques and explores the uses of liposomes as drug carriers but focuses primarily on liposomal carrier systems and technology in bioactive functional foods and nutraceuticals. The volume presents advances on liposomes as anti-tubercular and anticancer delivery systems and also discusses liposomal supplements. Liposomes for Functional Foods and Nutraceuticals: From Research to Application will be a valuable resource for those who produce lipids and those who seek to incorporate them into appropriate food products.
Download or read book Liposome Technology Second Edition written by Gregory Gregoriadis and published by CRC Press. This book was released on 1992-10-13 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposome Technology, Second Edition, is an updated, expanded new edition of a classic volume in the field. It covers all aspects of liposome technology, including liposome preparation and analysis, drug entrapment, and techniques used for in vivo and in vitro evaluation of liposomes. Leading authorities have contributed 70 chapters to create what is destined to be the standard liposome technology book for the 1990s. Many of the chapters describe methodologies practiced in authors' laboratories and provide specific examples of how these methodologies are applied in specific circumstances. Liposome Technology, Second Edition will be an essential reference volume for academic and industrial researchers in pharmacology, pharmacy, medicine, biochemistry, and immunology. What's new in the 2nd Edition? The 2nd Edition covers significant developments in liposome technology that have occurred since the publication of the 1st Edition in 1982. These developments include the following: New preparative procedures Special approaches to accommodate the incorporation of certain drugs New targeting techniques Large scale production of liposomes, patents, and clinical trials
Download or read book Liposomes written by Angel Catala and published by BoD – Books on Demand. This book was released on 2017-10-25 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes have received increased attention in recent years. Nevertheless, liposomes, due to their various forms and applications, require further investigation. These structures can deliver both hydrophilic and hydrophobic drugs. Preparation of liposomes results in different properties for these systems. In addition, there are many factors and difficulties that affect the development of liposome drug delivery structure. The purpose of this book is to concentrate on recent developments on liposomes. The articles collected in this book are contributions by invited researchers with a long-standing experience in different research areas. We hope that the material presented here is understandable to a broad audience, not only scientists but also people with general background in many different biological sciences. This volume offers you up-to-date, expert reviews of the fast-moving field of liposomes.
Download or read book Liposomes Part A written by Nejat Duzgunes and published by Elsevier. This book was released on 2003-11-11 with total page 345 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Methods in Liposome Preparation Physiochemical Characterization of Liposomes
Download or read book Liposomes written by Volkmar Weissig and published by Humana Press. This book was released on 2009-12-18 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that “the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer” [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.
