Download or read book Mass Spectrometry Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.
Download or read book Lipid Modifications of Proteins written by Milton J. Schlesinger and published by CRC Press. This book was released on 1992-11-30 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: The only comprehensive one-volume work describing protein-bound lipids Lipid Modifications of Proteins is the first single-volume publication to provide a comprehensive discussion of the five major kinds of protein-bound lipids. The book examines the biochemical activities involved in covalent attachment of different kinds of lipids to proteins, and it indicates the extent of lipid modifications to proteins. The book also thoroughly evaluates current hypotheses on roles of covalent lipids in protein structure and function. This one-of-a-kind volume is essential for molecular biologists, cell biologists, biochemists, biophysicists, microbiologists, and other researchers interested in the effect of lipids on proteins.
Download or read book Lipid Modification of Proteins written by N. M. Hooper and published by Oxford University Press, USA. This book was released on 1992 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipid Modification of Proteins: A Practical Approach is a unique guide to the latest methods is use, written by the acknowledged experts in the field. Detailed protocols are provided for all the key techniques, and the relevant background material is included. This book is an essential manual for a wide range of scientists studying the modification of protein by lipids, including membrane and protein biochemists, cell biologists, immunologists, bacteriologists, parasitologists, and virologists.
Download or read book Lipid Modifications of Proteins written by Patrick J. Casey and published by Academic Press. This book was released on 1995-05-16 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is organized around the three major classes of lipids that have been identified in covalent attachment to proteins in eukaryotic cells: isoprenoids, saturated fatty acyl groups and glycosylphosphatidylinositol ( GPI ).
Download or read book Membrane Dynamics and Domains written by Peter J. Quinn and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fluid-mosaic model of membrane structure formulated by Singer and Nicolson in the early 1970s has proven to be a durable concept in terms of the principles governing the organization of the constituent lipids and proteins. During the past 30 or so years a great deal of information has accumulated on the composition of various cell membranes and how this is related to the dif ferent functions that membranes perform. Nevertheless, the task of explaining particular functions at the molecular level has been hampered by lack of struc tural detail at the atomic level. The reason for this is primarily the difficulty of crystallizing membrane proteins which require strategies that differ from those used to crystallize soluble proteins. The unique exception is bacteriorhodopsin of the purple membrane of Halobacterium halobium which is interpolated into a membrane that is neither fluid nor in a mosaic configuration. To date only 50 or so membrane proteins have been characterised to atomic resolution by diffraction methods, in contrast to the vast data accumulated on soluble proteins. Another factor that has been difficult to explain is the reason why the lipid compliment of membranes is often extremely complex. Many hundreds of different molecular species of lipid can be identified in some membranes. Remarkably, the particular composition of each membrane appears to be main tained within relatively narrow limits and its identity distinguished from other morphologically-distinct membranes.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lipid Modification by Enzymes and Engineered Microbes written by Uwe T. Bornscheuer and published by Elsevier. This book was released on 2018-03-27 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipid Modification by Enzymes and Engineered Microbes covers the state-of-the art use of enzymes as natural biocatalysts to modify oils, also presenting how microorganisms, such as yeast, can be designed. In the past ten years, the field has made enormous progress, not only with respect to the tools developed for the development of designer enzymes, but also in the metabolic engineering of microbes, the discovery of novel enzyme activities, and in reaction engineering/process development. For the first time, these advances are covered in a single-volume that is edited by leading enzymatic scientist Uwe Borchscheuer and authored by an international team of experts. Identifies how, and when, to use enzymes and microbes for lipid modification Provides enzymatic, microbial and metabolic techniques for lipid modification Covers lipases, acyltransferases, phospholipases, lipoxygenases, monooxygenases, isomerases and sophorolipids Includes lipid modification for use in food, biofuels, oleochemicals and polymer precursors
Download or read book Biochemistry of Lipids Lipoproteins and Membranes written by Neale Ridgway and published by Elsevier. This book was released on 2015-07-24 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry of Lipids: Lipoproteins and Membranes, Volume Six, contains concise chapters that cover a wide spectrum of topics in the field of lipid biochemistry and cell biology. It provides an important bridge between broad-based biochemistry textbooks and more technical research publications, offering cohesive, foundational information. It is a valuable tool for advanced graduate students and researchers who are interested in exploring lipid biology in more detail, and includes overviews of lipid biology in both prokaryotes and eukaryotes, while also providing fundamental background on the subsequent descriptions of fatty acid synthesis, desaturation and elongation, and the pathways that lead the synthesis of complex phospholipids, sphingolipids, and their structural variants. Also covered are sections on how bioactive lipids are involved in cell signaling with an emphasis on disease implications and pathological consequences. Serves as a general reference book for scientists studying lipids, lipoproteins and membranes and as an advanced and up-to-date textbook for teachers and students who are familiar with the basic concepts of lipid biochemistry References from current literature will be included in each chapter to facilitate more in-depth study Key concepts are supported by figures and models to improve reader understanding Chapters provide historical perspective and current analysis of each topic
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Download or read book Biochemistry of Lipids Lipoproteins and Membranes written by Jean E. Vance and published by . This book was released on 1991 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Post translational Modification of Proteins by Lipids written by Urs Brodbeck and published by Springer. This book was released on 1988-10-17 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: The growing interest in recent years in the anchoring to membranes of proteins by post translational modification is documented by the large number of publications which ap peared in this field. In September 1987, scientists from 10 countries from all over the world met in the resort village of Les Diablerets, Switzerland, to discuss the most recent advances made in this field. The sessions were devoted to the anchoring of membrane proteins by cova lent attachment of fatty acids and of glycophospholipids. The workshop brought together many scientists working on vastly different proteins such as the variant surface glycoprotein of Trypanosomes and antigens of the mammalian cells. The subject of the workshop unified many scientists who had not met before and thus greatly stimulated interdisciplinary work. In addition to the lectures, each participant was provided with a collection of Methods currently in use in the study of membrane proteins anchored by post-translational modifica tion. An updated version of this collection is now presented as a Laboratory Manual, and the techniques described therein will give researchers easy and practical access to the investiga tion of post-translationally modified proteins. The publication of the present book by Springer follows an established tradition of previously published manuals on the handling of membrane proteins. Our thanks go to the authors who made the essential contribution in writing and adapting the experimental protocols, to Mrs. R.
Download or read book Protein Lipidation written by Maurine E. Linder and published by . This book was released on 2019 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Lipidation: Methods and Protocols is a valuable resource for experts in the field and for investigators who encounter protein lipidation through their research on a particular cellular process or favorite protein.
Download or read book Posttranslational Modification of Proteins written by Christoph Kannicht and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the posttranslational modifications of protein, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins.
Download or read book Enzymes in Lipid Modification written by Uwe Theo Bornscheuer and published by Wiley-Blackwell. This book was released on 2000-09-13 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic methods of lipid modification, particularly of fats and oils, have developed rapidly since the 1980s. In parallel to the rapid progress in research a wide range of applications have emerged, e.g. in the food industry. The book is written by leading experts in the field and reflects the state-of-the-art of enzymatic lipid modification. It provides the reader with guidelines how to select suitable enzymes and how to apply them efficiently. Applications of lipases and phospholipases, lipoxygenases and P450-monooxygenases and the use of whole-cell systems in lipid modification are described. Cloning, expression and mutagenesis as well as attempts to understand the molecular basis of specificity and stereoselectivity are outlined. In addition engineering aspects and the choice of solvent systems are addressed.
Download or read book The Enzymes of Biological Membranes written by A.N. Martonosi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the first edition of The Enzymes of Biological Membranes, published in four volumes in 1976, we collected the mass of widely scattered information on membrane-linked enzymes and metabolic processes up to about 1975. This was a period of transition from the romantic phase of membrane biochemistry, preoccupied with conceptual developments and the general properties of membranes, to an era of mounting interest in the specific properties of membrane-linked enzymes analyzed from the viewpoints of modem enzymology. The level of sophistication in various areas of membrane research varied widely; the structures of cytochrome c and cytochrome b5 were known to atomic detail, while the majority of membrane-linked enzymes had not even been isolated. In the intervening eight years our knowledge of membrane-linked enzymes ex panded beyond the wildest expectations. The purpose of the second edition of The Enzymes of Biological Membranes is to record these developments. The first volume describes the physical and chemical techniques used in the analysis of the structure and dynamics of biological membranes. In the second volume the enzymes and met abolic systems that participate in the biosynthesis of cell and membrane components are discussed. The third and fourth volumes review recent developments in active transport, oxidative phosphorylation and photosynthesis.
Download or read book Diet and Health written by National Research Council and published by National Academies Press. This book was released on 1989-01-01 with total page 765 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diet and Health examines the many complex issues concerning diet and its role in increasing or decreasing the risk of chronic disease. It proposes dietary recommendations for reducing the risk of the major diseases and causes of death today: atherosclerotic cardiovascular diseases (including heart attack and stroke), cancer, high blood pressure, obesity, osteoporosis, diabetes mellitus, liver disease, and dental caries.
Download or read book Biology for AP Courses written by Julianne Zedalis and published by . This book was released on 2017-10-16 with total page 1923 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology for AP® courses covers the scope and sequence requirements of a typical two-semester Advanced Placement® biology course. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology for AP® Courses was designed to meet and exceed the requirements of the College Board’s AP® Biology framework while allowing significant flexibility for instructors. Each section of the book includes an introduction based on the AP® curriculum and includes rich features that engage students in scientific practice and AP® test preparation; it also highlights careers and research opportunities in biological sciences.
