Download or read book Lipases and Phospholipases written by Georgina Sandoval and published by Humana. This book was released on 2019-12-10 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.
Download or read book Lipase written by B.K. Konwar and published by CRC Press. This book was released on 2018-05-23 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial lipases are industrially important and have gained attention due to their stability, selectivity, and broad substrate specificity. Lipases are used as medicine, and they also aid in indigestion, heartburn, allergy to gluten in wheat products (celiac disease), Crohn’s disease, and cystic fibrosis. This volume considers the industrial demand for new sources of lipases with different catalytic characteristics that stimulate the isolation, growth, and development of new microbial strains. The volume narrates the challenging metagenomic approach with the isolation of the lipase gene, its cloning into Escherichia coli, culture of the recombinant bacteria, and extraction and assessment of the lipase enzyme. Lipase-producing bacteria are available in different habitats, such as industrial wastes, vegetable oil processing factories, dairy plants, and soils contaminated with oil and oil seeds, among others. This volume is the effort of the authors to document the scientific findings carried out over the last eight years in the area of un-culturable soil microorganisms. The book presents the physic-chemical features of lipases and their specific applications in different commercial industries. The in-depth study looks at metagenomics for lipases from all angles and provides a truly informative resource. It describes the biochemical characterization of lipase enzymes with the high activity in the presence of 1% tributyrin. A wide review has been presented in the book on lipase enzymes purified from a large collection of microbes present in soil, seawater, waste-dumping sites, animal systems (including human beings), and the atmosphere. Stability of enzymes over changing environments of the industry is indeed a big issue, and the book deals at length with the changing temperatures and pH and metal ion concentrations.
Download or read book The Exocrine Pancreas written by Stephen Pandol and published by Morgan & Claypool Publishers. This book was released on 2011 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt: The secretions of the exocrine pancreas provide for digestion of a meal into components that are then available for processing and absorption by the intestinal epithelium. Without the exocrine pancreas, malabsorption and malnutrition result. This chapter describes the cellular participants responsible for the secretion of digestive enzymes and fluid that in combination provide a pancreatic secretion that accomplishes the digestive functions of the gland. Key cellular participants, the acinar cell and the duct cell, are responsible for digestive enzyme and fluid secretion, respectively, of the exocrine pancreas. This chapter describes the neurohumoral pathways that mediate the pancreatic response to a meal as well as details of the cellular mechanisms that are necessary for the organ responses, including protein synthesis and transport and ion transports, and the regulation of these responses by intracellular signaling systems. Examples of pancreatic diseases resulting from dysfunction in cellular mechanisms provide emphasis of the importance of the normal physiologic mechanisms.
Download or read book New Lipases and Proteases written by Abu Bakar Salleh and published by Nova Publishers. This book was released on 2006 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic processing of lipids and oils is becoming an important area of research. Hydrolytic enzymes, such as lipases and proteases are being sought after as the biocatalysts. This book focuses on the search and acquisition, isolation and purification and the characterisation of these enzymes
Download or read book Basic Concepts in Clinical Biochemistry A Practical Guide written by Vijay Kumar and published by Springer. This book was released on 2018-03-30 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
Download or read book Lipases written by Paul Woolley and published by Cambridge University Press. This book was released on 2012-01-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 1994 book provides a fascinating account of the fast-moving field of lipase research. The contributions, written by active research workers, summarise developments in the field and give access to recent literature. It covers both the lipases proper (triglyceride lipases and the phospholipases. It gives a comprehensive picture of the state of knowledge of these enzymes, with a strong bias towards the fields that are attracting the greatest attention: their detailed molecular structure, their mechanism of action, their position in the evolution of enzymes, and their application both in the laboratory and industry. The book will continue to be of interest to those working in universities, in research institutes and in companies specialising in biotechnology. The book will also be a useful reference book for postgraduate students entering this field of research.
Download or read book Industrial Enzymes written by Julio Polaina and published by Springer Science & Business Media. This book was released on 2007-05-16 with total page 629 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in genetic engineering and protein chemistry are bringing ever more powerful means of analysis to bear on the study of enzyme structure. This volume reviews the most important types of industrial enzymes. In a balanced manner it covers three interrelated aspects of paramount importance for enzyme performance: three-dimensional protein structure, physicochemical and catalytic properties, and the range of both classical and novel applications.
Download or read book Lipase and Phospholipase Protocols written by Mark Doolittle and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman's disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from any other enzyme because of the physical complexity of the environment in which catalysis occurs.
Download or read book Engineering Of with Lipases written by F.X. Malcata and published by Springer Science & Business Media. This book was released on 1996-03-31 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: The innovative uses of lipases in a wide variety of organic syntheses and the modification of existing fats and oils have increased exponentially over the last five years, due to the increasing availability of lipases from (genetically engineered) microbial sources, coupled with their special capacity to act as catalysts at hydrophilic/hydrophobic interfaces. As a result of the structural characterisation performed during the same period, applications of these lipases can now be developed in a much more rational way. Engineering of/with Lipases presents two major topics: the design and production of lipases with desired, preselected properties, and the use of lipases for desired applications. Audience: Doctoral and post-doctoral crystallographers, biochemists, geneticists and enzyme kineticists. Food, chemical and biochemical engineers. The former will learn about the practical aims and constraints associated with industrial applications of lipases, enabling them to design lipases for specific purposes. The latter will learn how to take advantage of the structural knowledge of lipases and their metabolic genesis to better design media, processes and products in terms of biochemical and technical feasibility.
Download or read book Nano and Biocatalysts for Biodiesel Production written by Avinash P. Ingle and published by John Wiley & Sons. This book was released on 2021-06-21 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reviews recent advances in catalytic biodiesel synthesis, highlighting various nanocatalysts and nano(bio)catalysts developed for effective biodiesel production Nano- and Biocatalysts for Biodiesel Production delivers an essential reference for academic and industrial researchers in biomass valorization and biofuel industries. The book covers both nanocatalysts and biocatalysts, bridging the gap between homogenous and heterogenous catalysis. Readers will learn about the techno-economical and environmental aspects of biodiesel production using different feedstocks and catalysts. They will also discover how nano(bio)catalysts can be used as effective alternatives to conventional catalysts in biodiesel production due to their unique properties, including reusability, high activation energy and rate of reaction, easy recovery, and recyclability. Readers will benefit from the inclusion of: Introductions to CaO nanocatalysts, zeolite nanocatalysts, titanium dioxide-based nanocatalysts and zinc-based in biodiesel production An exploration of carbon-based heterogeneous nanocatalysts for the production of biodiesel Practical discussions of bio-based nano catalysts for biodiesel production and the application of nanoporous materials as heterogeneous catalysts for biodiesel production An analysis of the techno-economical considerations of biodiesel production using different feedstocks Nano- and Biocatalysts for Biodiesel Production focuses on recent advances in the field and offers a complete and informative guide for academic researchers and industrial scientists working in the fields of biofuels and bioenergy, catalysis, biotechnology, bioengineering, nanotechnology, and materials science.
Download or read book Biotechnological Applications of Cold Adapted Organisms written by Rosa Margesin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: How can industry profit from the biochemical tricks of cold-adapted organisms? This book covers a range of aspects in this fascinating field, from genetic tools to environmental biotechnology.
Download or read book The Metabolic Molecular Bases of Inherited Disease written by Charles R. Scriver and published by New York ; Montreal : McGraw-Hill. This book was released on 2001 with total page 6338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents clinical, biochemical, and genetic information concerning those metabolic anomalies grouped under inborn errors of metabolism.
Download or read book Lipases written by Hannes Broeken and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this collection, the authors discuss endothelial lipase (EL), a recently identified member of the lipase gene family which exhibits a high degree of sequence similarity to lipoprotein lipase and hepatic lipase. The opening chapter summarizes the recent findings with regard to the molecular structure and biological mechanisms of EL, factors affecting the EL expression and activity as well as its role in some inflammatory disorders. Additionally, enzyme lipase is discussed; specifically its wide range of applications in the food, cosmetic, pharmaceutical as well as oleochemical industries. The capability of enzyme lipase to catalyse reactions with higher efficiency and stability, their chemo-, regio- and enantioselectivity properties, as well as their eco-friendly characteristics resulted in their high demand worldwide. Following this, the use of enzyme immobilization as an efficient strategy to overcome certain drawbacks is analyzed. Immobilization of lipases enhances properties such as thermostability and activity, facilitates separation of products, and provides more flexibility with enzyme/substrate contact by using various reactor configurations. This chapter will also highlight benefits of immobilization in lipase stabilization with respect to other employed methods such as chemical modification and recombinant DNA technology. In the concluding study, three commercially available lipases, namely Lipase PS-CI and Lipase PS-DI from Pseudomonas cepacia (non-specific lipase), and Lipozyme RM-IM from Rhizomucor miehei (1,3-regioselective lipase) were screened for their effect on catalyzing the acidolysis of tricaprylin with ALA. The effect of substrate molar ratio, temperature and time of reaction on incorporation of ALA were investigated and optimized by response surface methodology. The study reveals the potential of lipase-catalyzed acidolysis of palm oil with ALA as a suitable alternative to improve the nutritional properties and health-beneficial effects of edible oils.
Download or read book Applications of Lipases as Biocatalysts written by R. C. Hammond and published by CRC Press. This book was released on 1990 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen increased awareness of the possibility of using lipases for modification of lipids and related compounds. A major goal of application studies has been the development of systems allowing the efficient reaction of lipophilic and hydrophilic substrates with lipases. Ingenious reaction systems using immobilized, entrapped and retained lipases have been developed and some scaled up to industrial scale. This special issue includes contributions from established workers in the are of lipases and covers their characteristics and applications in reviews and original papers.
Download or read book Lingual and Gastric Lipases written by Margit Hamosh and published by CRC Press. This book was released on 2020-04-15 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1990: This book discusses the role of fat digestion with regards to Lingual and Gastric Lipases.
Download or read book Lipases and Phospholipases in Drug Development written by Günter Müller and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipases and Phospholipases are key control elements in mammalian metabolism. They share many common features that set them apart from other metabolic enzyme classes, most importantly their association with biological membranes. Their potential as drug targets for the treatment of metabolic diseases is widely recognized, and the first lipase inhibitor drugs have been successfully introduced. Providing drug developers with a firm foundation for lipase-centered drug design, the editors of this volume have assembled experts from different scientific disciplines to create a comprehensive handbook for all pharmaceutical chemists, biochemists and physiologists working with lipases. The authors examine fundamental aspects of lipase function in vitro and in vivo, explaining how this knowledge may be used to develop lipase assays. They also treat the physiological roles of lipases in normal and disordered metabolism, as well as strategies to target lipases for the treatment of diabetes, obesity and related disorders. Additional topics include the application of phospholipases for liposome-based drug delivery and their use as diagnostic tools.
Download or read book Esterases Lipases and Phospholipases written by M.I. Mackness and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: The NATO-Advanced Research Workshop "Esterases, Lipases and Phospholipases: From Structure to Clinical Significance" was held at the University of Bordeaux II, France from 22nd- 24th September 1993 under the Directorship of Professor Michel Clerc of the University of Bordeaux II. The meeting was organised by Hugues Chap (INSERM U 326, Toulouse, France), Georges Ferard (University of Strasbourg, France), Wolfgang Junge (University of Kiel, Germany) and Michael Mackness (University of Manchester, UK). In recent years it has become increasingly apparent that hydrolytic enzymes of the esterase, lipase and phospholipase type play central roles in the pathophysiology of many human diseases. The purpose of this NATO-ARW was to bring together experts (both clinical and scientific) in all three interrelated fields to review the current basic and clinical position and discuss future developments particularly with respect to future research aimed at determining the basic biochemical lesion involving hydrolytic enzymes involved in human disease and the use of these enzymes in diagnosis. As well as formal lectures from established researchers, the meeting also involved a number of lively round-table discussions on future developments and presentations from younger research workers, all of which are recorded in this Proceedings and which contribute to the success of the meeting.
