Download or read book Light Sheet Adaptive Optics Microscope for 3D Live Imaging written by Cyril Jules Tugdual Bourgenot and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical microscopy is still the main research tool for many biological studies. Indeed with the advent of genetic manipulation and specifically, the use of fluorescent protein expressing in animals and plants it has actually seen a renaissance in the past ten years, in particular with the development of novel techniques such as CARS, PALM, STORM, STED and SPIM. In all of microscopy methods one has to look through the sample at some point. The sample thus adds an additional and uncontrolled optical path, which leads to aberrations in the final image. Adaptive optics (AO) is a way of removing these unwanted aberrations which can cause image degradation and even potentially artifacts within the image. This thesis is concerned with the implementation of AO in non scanning microscopes and presents some novel methods both in wavefront sensored and sensorless configurations. A first implementation of AO on the emission path of a light sheet microscope is also presented.
Download or read book Light Sheet Microscopy Incorporating Adaptive Optics written by Keelan Michael Lawrence and published by . This book was released on 2018 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: When imaging deep into biological samples in Light Sheet Microscopy, refractive index variations within the sample, as well as differences between the sample, immersion medium, and sample holder distort the optical wavefront causing a loss of resolution and a decrease of the signal to noise ratio of the image. By implementing Adaptive Optics in a light sheet system, optical aberrations can be corrected and resolution can be increased despite the wavefront distortions cause by the organism. Adaptive Optics systems operate by sensing the wavefront of the incident light and then correcting the distortions in the wavefront with a modulation element. In this paper, we cover the design and implementation of an Adaptive Optics system composed of a deformable mirror and Shack-Hartmann Wavefront Sensor for the measurement and correction of wavefront aberrations caused by zebrafish (D. rerio) larvae during live imaging with Light Sheet Microscopy.
Download or read book Adaptive Optics for Biological Imaging written by Joel A Kubby and published by CRC Press. This book was released on 2013-04-26 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adaptive Optics for Biological Imaging brings together groundbreaking research on the use of adaptive optics for biological imaging. The book builds on prior work in astronomy and vision science. Featuring contributions by leaders in this emerging field, it takes an interdisciplinary approach that makes the subject accessible to nonspecialists who want to use adaptive optics techniques in their own work in biology and bioengineering. Organized into three parts, the book covers principles, methods, and applications of adaptive optics for biological imaging, providing the reader with the following benefits: Gives a general overview of applied optics, including definitions and vocabulary, to lay a foundation for clearer communication across disciplines Explains what kinds of optical aberrations arise in imaging through various biological tissues, and what technology can be used to correct for these aberrations Explores research done with a variety of biological samples and imaging instruments, including wide-field, confocal, and two-photon microscopes Discusses both indirect wavefront sensing, which uses an iterative approach, and direct wavefront sensing, which uses a parallel approach Since the sample is an integral part of the optical system in biological imaging, the field will benefit from participation by biologists and biomedical researchers with expertise in applied optics. This book helps lower the barriers to entry for these researchers. It also guides readers in selecting the approach that works best for their own applications.
Download or read book Light Sheet Fluorescence Microscopy written by Emmanuel G. Reynaud and published by John Wiley & Sons. This book was released on 2024-04-08 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: An indispensable guide to a novel, revolutionary fluorescence microscopy technique! Light sheet-based fluorescence microscopy has revolutionized microscopy, since it allows scientists to perform experiments in an entirely different manner and to record data that had not been accessible before. With contributions from noted experts in the fields of physics, biology, and computer science, Light Sheet Fluorescence Microscopy is a unique guide that offers a practical approach to the subject, including information on the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis. Comprehensive in scope, the book is filled with the cutting-edge methods as well as valuable insider tips. Grounded in real-world applications, the book includes chapters from major manufacturers that explores their recent systems and developments. In addition, the book hightlights a discussion of a “do-it-yourself” light sheet microscope, making the technique affordable for every laboratory. This important book: Serves as an easy-to-understand introduction to light sheet-based fluroescence Includes numerous tips and tricks for advanced practitioners Provides in-depth information on hardware and software solutions for a straightforward implementation of light sheet fluorescence microscopy in the lab Includes chapters from the major manufacturers including Zeiss, Leica, Lavision Biotech, Phase View, and Asi Aimed at cell biologists, biophysicists, developmental biologists, and neurobiologists, Light Sheet-based Fluorescence Microscopy offers a comprehensive overview of the and the most recent applications of this microscopy technique.
Download or read book Fluorescence Microscopy written by Anda Cornea and published by Elsevier. This book was released on 2014-02-24 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. - Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research - Offers insight into common challenges in implementing techniques, as well as effective solutions
Download or read book Fundamentals of Light Microscopy and Electronic Imaging written by Douglas B. Murphy and published by John Wiley & Sons. This book was released on 2012-08-22 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
Download or read book Multi Parametric Live Cell Microscopy of 3D Tissue Models written by Ruslan I. Dmitriev and published by Springer. This book was released on 2017-10-26 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
Download or read book Adaptive Optics and Wavefront Control for Biological Systems written by Thomas G. Bifano and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Live Cell Optical Microscopy with Limited Light Dose Methods and applications 2 1 Cell cultivation 2 2 Wide field microscopy 2 3 Confocal laser scanning microscopy 2 4 Light sheet fluorescence microscopy 2 5 Structured illumination microscopy 2 6 Axial tomography 2 7 Total internal reflection fluorescence microscopy 2 8 Spectral imaging FLIM and FRET written by Herbert Schneckenburger and published by . This book was released on 2018 with total page 38 pages. Available in PDF, EPUB and Kindle. Book excerpt: Live-cell microscopy is a rapidly growing field for the detection of cell or tissue architecture and for cell metabolism. This Spotlight examines the early detection of diseases and cellular responses to pharmaceutical agents with 3-D systems and non-phototoxic light doses. Techniques discussed in this eBook include 3-D microscopy, super-resolution microscopy, membrane imaging, long-exposure imaging, and spectral imaging.
Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Download or read book Quantitative Imaging in Cell Biology written by and published by Academic Press. This book was released on 2014-06-25 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Download or read book Developments in Surface Contamination and Cleaning Volume 12 written by Rajiv Kohli and published by Elsevier. This book was released on 2019-06-08 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developments in Surface Contamination and Cleaning: Methods for Assessment and Verification of Cleanliness of Surfaces and Characterization of Surface Contaminants, Volume Twelve, the latest release in the Developments in Surface Contamination and Cleaning series, provides best practices on determining surface cleanliness. Chapters include an introduction to the nature and size of particles, a discussion of cleanliness levels, detailed coverage of measurement methods, characterization methods and analytical methods for evaluating surfaces, and an overview of analysis methods for various contaminants. As a whole, the series creates a unique and comprehensive knowledge base for those in research and development in a variety of industries. Manufacturing, quality control and procurement specification professionals in the aerospace, automotive, biomedical, defense, energy, manufacturing, microelectronics, optics and xerography industries will find this book to be very helpful. In addition, researchers in an academic setting will also find these volumes excellent source books. - Includes an extensive listing, with a description of available methods for the assessment of surface cleanliness - Provides a single source of information on methods for verification of surface cleanliness - Serves as a guide to the selection, assessment and verification of methods for specific applications
Download or read book Optical Measurements for Scientists and Engineers written by Arthur McClelland and published by Cambridge University Press. This book was released on 2018-04-19 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: An accessible, introductory text explaining how to select, set up and use optical spectroscopy and optical microscopy techniques.
Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Download or read book Adaptive Optics Wide field Microscopy Using Direct Wavefront Sensing written by Oscar A. Azucena and published by . This book was released on 2011 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Principles of Light Microscopy From Basic to Advanced written by Volodymyr Nechyporuk-Zloy and published by Springer Nature. This book was released on 2022-11-29 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook is an excellent guide to microscopy for students and scientists, who use microscopy as one of their primary research and analysis tool in the laboratory. The book covers key microscopy principles and explains the various techniques such as epifluorescence microscopy, confocal/live cell imaging, SIM/light sheet microscopy, and many more. Easy-to-understand protocols provide helpful guidance for practical implementation in various commercially available imaging systems. The reader is introduced to histology and further be guided through advanced image acquisition, classification and analysis. The book is written by experienced imaging specialists from the UK, other EU countries, the US and Asia, and is based on advanced training courses for master students and PhD students. Readers are not expected to be familiar with imaging and microscopy technologies, but are introduced to the subject step by step. This textbook is indented for biomedical and medical students, as well as scientists and postdocs who want to acquire a thorough knowledge of microscopy, or gain a comprehensive overview of modern microscopy techniques used in various research laboratories and imaging facilities. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Light Sheet Based Fluorescence Microscopy written by Ernst H. K. Stelzer and published by Wiley-Blackwell. This book was released on 2013-08-05 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book introducing a revolutionary new imaging technology, light sheet fluorescence microscopy. Written in a comprehensive fashion by the same people who developed this technique, this treatise is a must have for everyone who plans to work with the new technology.
