Download or read book Le Mode d imagerie wet STEM written by Agnès Bogner and published by . This book was released on 2006 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ce travail de thèse explore les potentialités d'un nouveau mode d'imagerie dévéloppé en microscopie à balayage environnementale (ESEM). L'ESEM constitue une des principales évolutions de la microscopie électronique à balayage ces dernières années. La présence d'un environnement gazeux au sein de l'enceinte du microscope permet de s'affranchir des restrictions liées au haut vide nécessaire à la microscopie conventionnelle. Il devient ainsi possible d'observer des échantillons hydratés, liquides et non conducteurs dans leur état natif et sans préparation préalable. Exploré en mode hydraté pour l'observation d'échantillons liquides comme les émulsions, ce type de microscopie a montré de fortes potentialités. Toutefois, des limites ont parfois été rencontrées en terme de contraste et de résolution (au mieux 50 à 200 nm). Le développement du mode wet-STEM, le mode STEM (microscopie électronique à balayage en transmission) utilisé en ESEM, a pour objectif d'étendre les possibilités de caractérisation d'échantillons liquides à l'échelle nanométrique et d'améliorer les contrastes obtenus. Il s'agit d'observer en transmission un film fin de liquide contenant des objets nanométriques à micrométriques, en utilisant une détection de type champ sombre annulaire à grands angles. Dans ce mode, une résolution de 5 nm est atteinte sur des nanoparticules d'or en suspension dans l'eau. Par ailleurs, un contraste de type masse-épaisseur, mis en évidence sur les images, est vérifié par modélisation Monte Carlo. Les images sont parfois plus complexes à interpréter : des phénomènes d'inversion de contraste sont rencontrés, attribués à la variation de l'épaisseur de liquide ; des phénomènes de dégradation liés à l'impact du faisceau électronique peuvent fortement modifier les images obtenues. Les potentialités révélées du wet-STEM sont alors mises à profit pour l'étude d'échantillons liquides plus complexes tels que des mini-émulsions monomère-eau et des latex, et confrontées aux techniques habituelles de caractérisation. Tout d'abord, les images wet-STEM permettent d'estimer la taille et la polydispersité des objets en suspension. Ensuite, l'influence des différents paramètres sur les observations en wet-STEM de latex est explorée : température de transition vitreuse du polymère constituant les particules, présence de surfactant, ajout de charges par mélange ou encapsulation, greffage réalisé sur des particules de latex par modification chimique. Quelques observations en condition d'évaporation ouvrent des perspectives quant à l'étude de la filmification in situ
Download or read book Make Life Visible written by Yoshiaki Toyama and published by Springer Nature. This book was released on 2019-10-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.
Download or read book Advances in Electronics and Electron Physics written by L. Marton and published by . This book was released on 1956 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quantitative Imaging in Cell Biology written by and published by Academic Press. This book was released on 2014-06-25 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Download or read book Progress in Clinical and Biological Research written by George J. Brewer and published by . This book was released on 1975 with total page 748 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Microscopy of Plant Cells written by C Hawes and published by Academic Press. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Download or read book Radiation Protection in Radiology large Facilities written by Canada. Health Canada and published by . This book was released on 2008 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Movements written by Dennis Bray and published by Garland Science. This book was released on 2001 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book vividly describes how complex and integrated movements can arise from the properties and behaviors of biological molecules. It provides a uniquely integrated account in which the latest findings from biophysics and molecular biology are put into the context of living cells. This second edition is updated throughout with recent advances in the field and has a completely revised and redrawn art program. The text is suitable for advanced undergraduates, graduate students, and for professionals wishing for an overview of this field.
Download or read book Color and Colorimetry Multidisciplinary Contributions written by Maurizio Rossi and published by . This book was released on 2012 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Giovanni Aurelio Augurello 1441 1524 and Renaissance Alchemy written by Matteo Soranzo and published by BRILL. This book was released on 2019-12-09 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first in-depth study of the life and works of Augurello, Italian alchemist, poet and art connoisseur from the time of Giorgione.
Download or read book Principles and Practice of Variable Pressure Environmental Scanning Electron Microscopy VP ESEM written by Debbie Stokes and published by John Wiley & Sons. This book was released on 2008-12-22 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a simple starting point to VPSEM, especially for new users, technicians and students containing clear, concise explanations Crucially, the principles and applications outlined in this book are completely generic: i.e. applicable to all types of VPSEM, irrespective of manufacturer. Information presented will enable reader to turn principles into practice Published in association with the Royal Microscopical Society (RMS) -www.rms.org.uk
Download or read book Studies in the Iconography of Northwest Semitic Inscribed Seals written by Benjamin Sass and published by Saint-Paul. This book was released on 1993 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Scanning Electron Microscopy and X Ray Microanalysis written by Joseph Goldstein and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.
Download or read book Bioimaging written by Douglas E. Chandler and published by Jones & Bartlett Publishers. This book was released on 2009 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.
Download or read book Vulvar Disease written by Jacob Bornstein and published by Springer. This book was released on 2019-04-13 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on modern approaches to vulvar lesions, taking into account recent management recommendations and employing the consensus terminology of the International Society for the Study of Vulvovaginal Disease (ISSVD). Further, it breaks with past misconceptions and myths, and explains in detail the modern approach. The aim is to help clinicians perform the differential diagnosis of vulvar conditions and implement the new recommended treatments. The core chapters of the book are arranged in accordance with lesion presentation: red lesions, white lesions, patches and plaques, papules and nodules, erosions and ulcers, blisters, etc. For each lesion, the multidisciplinary management is fully explained, including clarifications of the role of gynecologists, dermatologists, physical therapists, pain specialists, sex therapists, and others. Clinical case presentations and numerous illustrations of treatment procedures are included, supplemented by informative online videos. A separate chapter is devoted to vulvar pain, which is increasingly becoming recognized as a growing problem. Here, again the past approaches are replaced with new paradigms. All of the authors are acknowledged experts in the field and the editor is a past president of the ISSVD. The book will be of value for all vulvar specialists and a wide range of other clinicians.
Download or read book European Russian Space Cooperation written by Brian Harvey and published by Springer Nature. This book was released on 2021-04-10 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: The story of European-Russian collaboration in space is little known and its importance all too often understated. Because France was the principal interlocutor between these nations, such cooperation did not receive the attention it deserved in English-language literature. This book rectifies that history, showing how Russia and Europe forged a successful partnership that has continued to the present day. Space writer Brian Harvey provides an in-depth picture of how this European-Russian relationship evolved and what factors—scientific, political and industrial—propelled it over the decades. The history begins in the cold war period with the first collaborative ventures between the Soviet Union and European countries, primarily France, followed later by Germany and other European countries. Next, the chapters turn to the missions when European astronauts flew to Russian space stations, the Soyuz rocket made a new home in European territory in the South American jungle and science missions were flown to study deep space. Their climax is the joint mission to explore Mars, called ExoMars, which has already sent a mission to Mars. Through this close examination of these European-Russian efforts, readers will appreciate an altogether new perspective on the history of space exploration, no longer defined by competition, but rather by collaboration and cooperation.
Download or read book General Relativity and Gravitational Waves written by Joseph Weber and published by Courier Corporation. This book was released on 2004-01-01 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: An internationally famous physicist and electrical engineer, the author of this text was a pioneer in the investigation of gravitational waves. Joseph Weber's General Relativity and Gravitational Waves offers a classic treatment of the subject. Appropriate for upper-level undergraduates and graduate students, this text remains ever relevant. Brief but thorough in its introduction to the foundations of general relativity, it also examines the elements of Riemannian geometry and tensor calculus applicable to this field. Approximately a quarter of the contents explores theoretical and experimental aspects of gravitational radiation. The final chapter focuses on selected topics related to general relativity, including the equations of motion, unified field theories, Friedman's solution of the cosmological problem, and the Hamiltonian formulation of general relativity. Exercises. Index.
