Download or read book Laser Imaging and Manipulation in Cell Biology written by Francesco S. Pavone and published by John Wiley & Sons. This book was released on 2011-07-28 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here, the editor has gathered a team of international experts to present the latest advances in the field of laser imaging and manipulation techniques. The result is broad coverage of the interactions with biological samples to perform novel optical manipulation operations, both on the cellular and tissue levels. Of interest to physicists working and researching laser tissue mechanisms, cell biologists investigating new imaging and manipulation operation on the cellular level, medical doctors working with new laser therapies and diagnostic tools, as well as engineers developing new technologies in the field of optics and lasers.
Download or read book Laser Manipulation of Cells and Tissues written by Michael W. Berns and published by Academic Press. This book was released on 2007-06-20 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. Laser Manipulation of Cells and Tissues ties all these systems and studies together, describing all of the different kinds of research and practical/analytical applications of laser manipulation. It also provides the reader with the basic information needed to actually build one's own laser micro-manipulation system. The combination of imaging and molecular probe technology with laser micromanipulation greatly extends the use of this technology in molecular, cellular, developmental and patho-biology/medicine. This book should be valuable to scientists, clinicians, and students in the fields of cell and developmental biology, cell physiology, cancer biology, pathology, and stem cell biology. Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis - a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchers
Download or read book Pulsed Laser Microbeams for Cellular Manipulation written by Amy Noel Stacy Hellman and published by ProQuest. This book was released on 2008 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highly focused pulsed laser radiation (pulsed laser microbeams) provide the ability to deposit energy with high spatial precision and controllable cellular damage. As a result, pulsed laser microbeams have been explored as a fast, non-contact means for cellular manipulations such as cellular microsurgery, transient cell membrane permeabilization, and targeted cell lysis. In this dissertation we examine the mechanisms of highly focused laser microbeams of nanosecond and picosecond duration to achieve cell lysis, cell necrosis, and molecular delivery. We have developed a time-resolved imaging system to visualize these processes with nanosecond temporal resolution and use image analysis to measure the physical perturbation applied to the cells as a function of laser microbeam pulse energy and pulse duration. Fluorescence assays are used to assess the biological response (necrosis, molecular delivery, and biochemical pathway activity) to the laser microbeam irradiation, and a biophysical model is developed to establish connections between specific physical characteristics and the resulting cellular effect. Our studies reveal that pulsed laser microbeam processes are mediated by optical breakdown resulting in plasma formation, shock wave emission, and cavitation bubble formation, expansion, and collapse. Cavitation bubble expansion was found to be the primary mechanism responsible for cellular modification. Hydrodynamic analysis based on the measured time evolution of the cavitation bubble growth, combined with assessment of the cellular response, revealed that the maximum wall shear stress associated with the cavitation bubble expansion governs the location and spatial extent of cell lysis, cell necrosis, and molecular delivery. In addition, we demonstrate how the variation of laser microbeam pulse duration can allow modulation of the spatial extent of cellular modification in order to tailor the cellular perturbations and optimize specific applications. These detailed studies provide a basis for the informed selection of specific laser parameters (i.e. pulse duration and energy) to achieve a desired cellular outcome, with controllable degrees of collateral damage. We demonstrate three different applications in which the laser parameters are well matched for particular applications in cellular analytics, activation of biochemical pathways, and microfluidics.
Download or read book Laser Tweezers in Cell Biology written by and published by Academic Press. This book was released on 1997-11-24 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer.* * Provides a practical laboratory guide for methods and technologies used with laser tweezers* Includes comprehensive and easy-to-follow protocols
Download or read book Micromanipulation by Light in Biology and Medicine written by Karl Otto Greulich and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are probably few people who do not dream of the good old times, when do ing science often meant fascination, excitement, even adventure. In our time, do ing science involves often technology and, perhaps, even business. But there are still niches where curiosity and fascination have their place. The subject of this book, technological as its title may sound, is one of the fortunate examples. It will report on lasers generating the coldest places in the Universe, and on table top laser microtools which can produce a heat "inferno" as it prevails in the interior of the Sun, or simulate, for specific plant cells, microgravity of the space around our plan et Earth. There will be some real surprises for the reader. The applications range from basic studies of the driving forces of cell division (and thus life) via genetic modification of cells (for example, for plant breeding) to medical applications such as blood cell analysis and finally in vitro fertilization. What are these instruments: laser microbeams and optical tweezers? Both are lasers coupled with a fluorescence microscope. The laser microbeam uses a pulsed ultraviolet laser. Light is focused, as well as possible, in space and time, in order to obtain extremely high light intensities - high enough to generate, for a very short instant, extremely hot spots which can be used to cut, fuse or perforate biological material.
Download or read book Imaging Cell Signaling written by Christoph Wülfing and published by Springer Nature. This book was released on with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lasers and Current Optical Techniques in Biology written by Giuseppe Palumbo and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of innovative light sources, fibre laser sources and light emitting diodes, is opening unexpected perspectives into optical techniques and is promising new exciting applications in the field of biomedicine. Lasers and Current Optical Techniques in Biology aims to provide an overview of light sources, together with an extensive and authoritative description of the optical techniques in bio-medicine. This book is designed to give biomedical researchers a strong feel for the capability of physical approaches, promote new interdisciplinary interests and persuade more practitioners to take advantage of optical techniques. Current developments in a variety of optical techniques, including Near-Infra Red Spectroscopy, and traditional and advanced fluorescence techniques are covered, ranging from those that are becoming common practice to those that need much more experimentation before they can be accepted as real breakthroughs. Further topics include optical coherence tomography and its variations, polarised light imaging and, principle laser and lamp sources- a usually fragmentary topic, often dispersed among specialist publications. The wide range of topics covered make Lasers and Current Optical Techniques in Biology of interest to a diverse range of scientific communities.
Download or read book Proceedings of Functional Imaging and Optical Manipulation of Living Cells written by International Biomedical Optics Society and published by SPIE-International Society for Optical Engineering. This book was released on 1997 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laser Manipulation of Cells and Tissues written by Michael W. Berns and published by Academic Press. This book was released on 2007-07-04 with total page 792 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. Laser Manipulation of Cells and Tissues ties all these systems and studies together, describing all of the different kinds of research and practical/analytical applications of laser manipulation. It also provides the reader with the basic information needed to actually build one's own laser micro-manipulation system. The combination of imaging and molecular probe technology with laser micromanipulation greatly extends the use of this technology in molecular, cellular, developmental and patho-biology/medicine. This book should be valuable to scientists, clinicians, and students in the fields of cell and developmental biology, cell physiology, cancer biology, pathology, and stem cell biology. Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis - a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchers
Download or read book Imaging from Cells to Animals In Vivo written by Margarida Barroso and published by CRC Press. This book was released on 2020-12-03 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.
Download or read book The Use of Optical Tweezers and Laser Scissors in Cell Biology written by Nicholas W. Hyun and published by . This book was released on 2014 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical tweezers and laser scissors are invaluable tools to manipulate cells on the micro and nano scale level. By integrating laser technologies with imaging and biochemical techniques, robust systems can be created to study various biological and biochemical processes. The purpose of this thesis is to describe two different optical methods used to study two cell-based problems. In the first experiment, optical tweezers are used to examine the effects of viscosity on sperm motility and energetics using a 1064nm Nd:YVO4 continuous laser. In the second experiment, laser scissors are used to study growth cone response from laser induced damage using a Vanguard Nd: YVO4 second harmonic generator (SHG) 532 nm picosecond green laser. The proceeding sections describe the optical systems for each experiment as well as the methods used. Results of both studies are presented and discussed in detail.
Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Download or read book Robotics for Cell Manipulation and Characterization written by Changsheng Dai and published by Elsevier. This book was released on 2023-04-20 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Robotics for Cell Manipulation and Characterization provides fundamental principles underpinning robotic cell manipulation and characterization, state-of-the-art technical advances in micro/nano robotics, new discoveries of cell biology enabled by robotic systems, and their applications in clinical diagnosis and treatment. This book covers several areas, including robotics, control, computer vision, biomedical engineering and life sciences using understandable figures and tables to enhance readers' comprehension and pinpoint challenges and opportunities for biological and biomedical research. - Focuses on, and comprehensively covers, robotics for cell manipulation and characterization - Highlights recent advances in cell biology and disease treatment enabled by robotic cell manipulation and characterization - Provides insightful outlooks on future challenges and opportunities
Download or read book Laser Capture in Microscopy and Microdissection written by P. Michael Conn and published by Academic Press. This book was released on 2002-10-21 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic principles; Specialized uses; Genetic applications.
Download or read book Confocal Microscopy written by Stephen W. Paddock and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.
Download or read book Laser Microirradiation of Cells written by Takahiro Kasuya and published by Routledge. This book was released on 1989 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: First volume in a new series designed to provide information quickly on current as well as promising developments in lasers and consisting of self-contained tracts and handbooks pertinent to laser science and technology. Five color plates. Annotation copyrighted by Book News, Inc., Portland, OR
Download or read book Imaging Manipulation and Analysis of Biomolecules Cells and Tissues IX written by Daniel L. Farkas and published by SPIE-International Society for Optical Engineering. This book was released on 2011 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes Proceedings Vol. 7821
