Download or read book Laboratory Methods in Vesicular and Vectorial Transport written by Alan Tartakoff and published by Academic Press. This book was released on 2012-12-02 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Methods in Vesicular and Vectorial Transport describes the procedures used to study the mechanisms of vesicular transport along the secretory and endocytic paths, including electron microscopy, autoradiography, and methods associated with cyto- and immunocytochemistry, genetics, and biochemistry. It investigates vectorial transport to the cisternal space of the rough endoplasmic reticulum (ER), as well as protein translocation across the ER, strategies for gaining access to the cytoplasm, cell-free analysis of vesicle fusion, the structure of glycoproteins, and the use of cell systems for analysis of vesicular traffic. Organized into seven parts encompassing 20 chapters, this volume begins with an overview of protein topology in the ER and the use of cross-linking methods to probe the molecular environment of translocating polypeptide chains. It then discusses the reconstitution of secretory protein translocation from detergent-solubilized rough microsomes; the use of anti-idiotype antibodies to characterize protein-protein interactions; the use of perforated cells to elucidate intracellular membrane transport; delivery of macromolecules into cells expressing a viral membrane fusion protein; and digitonin permeabilization procedures for studying endosome acidification and function. The reader is also introduced to reconstitution of intracellular vesicle fusion in a cell-free system after receptor-mediated endocytosis; immunoisolation using magnetic solid supports; endosome and lysosome purification by free-flow electrophoresis; remodeling of glycoprotein oligosaccharides after endocytosis; and replica plating of animal cells. This book will interest students, researchers, geneticists, biochemists, and cell biologists.
Download or read book Methods in Plant Cell Biology Part A written by and published by Elsevier. This book was released on 1995-10-10 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants
Download or read book Methods for Analysis of Golgi Complex Function written by and published by Elsevier. This book was released on 2013-11-26 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Download or read book Neurons Methods and Applications for the Cell Biologist written by Peter J. Hollenbeck and published by Gulf Professional Publishing. This book was released on 2003-07-08 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many cell biologists study the properties of cells grown outside of the animal, in the controlled environment of a culture dish. Many cell biologists would benefit from directing their attention to nerve cells, but are afraid to try growing nerve cells in culture because it has the reputation for being much more difficult and unreliable than growing other kinds of cells. This volume is written by cell biologists and neuroscientists who are experts at growing various kinds of neurons in culture and using them for diverse experimental applications. The aim of this volume is to demystify the techniques used to grow nerve cells and to show cell biologists that it is something that they can easily master. * Contains culture methodology for more than a dozen types of CNS and PNS neurons * Includes most recent and reliable techniques from expert practitioners for specific experimental applications * Addresses the latest strategies for transfecting neurons.
Download or read book Recent Advances in Cytometry Part A written by and published by Academic Press. This book was released on 2011-06-13 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays Discusses many new assays developed since the previous edition Presents recent developments in cytometric intrumentation/technology
Download or read book Biophysical Tools for Biologists written by and published by Academic Press. This book was released on 2009-01-19 with total page 739 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method
Download or read book Biophysical Tools for Biologists written by John J. Correia and published by Academic Press. This book was released on 2011-09-21 with total page 996 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method Includes full-color plates to illustrate techniques
Download or read book Development of Sea Urchins Ascidians and Other Invertebrate Deuterostomes Experimental Approaches written by and published by Elsevier. This book was released on 2004-11-16 with total page 909 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a practical guide to experimental methods for studying the development invertebrate deuterostomes as animal model systems. The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches. * Covers a wide range of methods, from classical embryology through modern genomics * Discusses animals related to vertebrates, providing a valuable evolutionary perspective * Includes a practical guide to the use of sea urchins in the teaching laboratory
Download or read book Microtubules in vitro written by John J. Correia and published by Academic Press. This book was released on 2010-07-03 with total page 722 pages. Available in PDF, EPUB and Kindle. Book excerpt: There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect both experimental procedures and new developments in the field of microtubule in vitro research Combines classical approaches and modern technologies Presents easy-to-use protocols and thorough background information, compiled by leaders in the field
Download or read book Lipids written by and published by Academic Press. This book was released on 2012-03-28 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipids are a broad group of naturally occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The main biological functions of lipids include energy storage, as structural components of cell membranes, and as important signaling molecules. This volume of Methods in Cell Biology covers such areas as Membrane structure and dynamics, Imaging, and Lipid Protein Interactions. It will be an essential tool for researchers and students alike. Covers such areas as membrane structure and dynamics, imaging, and lipid protein interactions An essential tool for researchers and students alike International authors Renowned editors
Download or read book The Zebrafish Cellular and Developmental Biology written by and published by Elsevier. This book was released on 2004-12-07 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Cell Biology, the first of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls Illustrates many techiques in full-color Summarizes the Zebrafish Genome Project
Download or read book Nuclear Structure and Function written by Miguel Berrios and published by Academic Press. This book was released on 1998 with total page 659 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a comprehensive guide to the methodologies used in the study of structural domains of cell nuclei. The text covers chromatin, the karyoskeleton, the soluble domain, and the nucleolus. It details methods that are used to isolate components from these domains and techniques used to assemble and disassemble nuclear elements. There is also coverage of three-dimensional mapping and localization of nuclear processes. Key Features * Provides a practical laboratory guide for studying cell nuclei * Includes comprehensive and easy-to-follow protocols
Download or read book Micropatterning in Cell Biology Part A written by and published by Elsevier. This book was released on 2014-01-16 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Cell Biology looks at micropatterning in cell biology and includes chapters on protein photo-patterning on PEG with benzophenone, laser-directed cell printing and dip pen nanolithography. The cutting-edge material in this comprehensive collection is intended to guide researchers for years to come. Includes sections on micropatterning in 2D with photomask, maskless micropatterning and 2D nanopatterning Chapters are written by experts in the field Cutting-edge material
Download or read book Lysosomes and Lysosomal Diseases written by and published by Academic Press. This book was released on 2015-02-04 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Cell Biology looks at methods for lysosomes and lysosomal diseases. Chapters focus upon practical experimental protocols to guide researchers through the analysis of multiple aspects of lysosome biology and function. In addition, it details protocols relevant to clinical monitoring of patients with lysosomal diseases. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Download or read book Centrosomes and Spindle Pole Bodies written by Robert E. Palazzo and published by Elsevier. This book was released on 2001-09-07 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Containing a comprehensive collection of convenient and quantitative methods for studying centrosomes, spindle pole bodies and related organelles, this text is a valuable resource for researchers and others interested in studying the role of these organelles in cell replication. Chapters outlining the role of these organelles in other cell functions are also included, and a wide variety of experimental systems for analyzing these organelles are presented. Detailed protocols for experiments are contained in each chapter for researchers to perform in their own labs. This volume outlines key methodologies used to analyze centrosomes and spindle pole bodies, their replication, and reproduction in the clear, well-illustrated style of the Methods in Cell Biology series.
Download or read book Apoptosis written by and published by Elsevier. This book was released on 2001-05-30 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Apoptosis provides a current and comprehensive collection of methods for the study of cell death. Using a diverse range of technical approaches and model systems, the chapters in this volume cover topics from the cellular and organismal to the molecular and anatomical. The methods are illustrated with user-friendly recipes and over 100 tables, halftones, and diagrams. Current methodologies for studying cell death Wide range of model systems Molecular, biochemical, cellular, and genetic approaches Complements the original Cell Death volume Up-to-date methodology for a fast moving field Designed with the needs of both basic scientists and clinicians in mind Authors are leaders in their respective fields
Download or read book Micropatterning in Cell Biology Part B written by and published by Elsevier. This book was released on 2014-01-27 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Cell Biology is the second volume describing micropatterning, complementing Volume 120. Chapters are written by experts in the field and include cutting-edge material. Includes sections on micropatterning in 2D with photomask, maskless micropatterning and 2D nanopatterning Chapters are written by experts in the field Cutting-edge material
