Download or read book Laboratory Manual Of Biochemistry written by R.S.Sengar and published by New India Publishing Agency. This book was released on 2014-01-15 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book "Laboratory Manual of Biochemistry: Methods and Techniques" is the outcome of 17 years of teaching and research experience of the authors. Biochemistry is a comparatively recent branch but the utility and variability of research work and the dazzling pace of its development has positioned this discipline in the forefront of scientific hierarchy. As Biochemistry works at a molecular level (i.e. finer than that accessed by the ultra-modern optical or phase-contrast microscopes) it embraces other disciplines also. Biochemistry has thus strengthened the integrated approach concept and solving biological riddles. Biochemical Techniques are used in all branches of biological sciences and biotechnology. Biochemical experiments are conducted in the laboratory as practical as well as for persuing research. A researcher has to refer to many journals and books before he/she could get to the working protocol for his/her experiment. This book attempts to give often-used methods in a single volume. This first edition is divided into 11 Units. Each experiment includes principle, requirements, procedure, calculation and observations. At the end of each , references for additional reading are provided. Important precautions, warnings and tips are given under the notes section. In addition, there are 12 appendices, which give minute details on basic chemistry, buffer preparations and other aspects required for the conduct of the experiments. The methods given in the book will be useful for conducting practical classes at the undergraduate and postgraduate levels in biochemistry, biotechnology, microbiology, agricultural sciences, environmental science, botany, zoology, nutrition, pharmaceutical science and other biology-related subjects. This book will be a bonanza for the research workers since it covers procedures from the classical basic biochemistry to the modern PCR techniques.
Download or read book Biochemistry Laboratory Manual For Undergraduates written by Timea Gerczei Fernandez and published by Walter de Gruyter GmbH & Co KG. This book was released on 2015-03-11 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
Download or read book Fundamental Laboratory Approaches for Biochemistry and Biotechnology written by Alexander J. Ninfa and published by John Wiley & Sons. This book was released on 2009-05-26 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Download or read book Biochemistry Laboratory written by Rodney F. Boyer and published by Pearson. This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The biochemistry laboratory course is an essential component in training students for careers in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Increasingly, many biochemistry lab instructors opt to either design their own experiments or select them from major educational journals. Biochemistry Laboratory: Modern Theory and Techniques addresses this issue by providing a flexible alternative without experimental protocols. Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.
Download or read book Biochemistry in the Lab written by Benjamin F. Lasseter and published by CRC Press. This book was released on 2019-09-30 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.
Download or read book Magnetic Cell Separation written by and published by Elsevier. This book was released on 2011-08-31 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation Describes the parameters used to determine cell magnetophoresis
Download or read book FRET and FLIM Techniques written by Theodorus W. J. Gadella and published by Elsevier. This book was released on 2011-07-29 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
Download or read book Laboratory Guide to the Methods in Biochemical Genetics written by Nenad Blau and published by Springer Science & Business Media. This book was released on 2008-05-31 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual deals specifically with laboratory approaches to diagnosing inborn errors of metabolism. The key feature is that each chapter is sufficiently detailed so that any individual can adopt the described method into their own respective laboratory.
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by T. S. Work and published by . This book was released on 1969 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laboratory Methodology in Biochemistry written by Carlo Fini and published by CRC Press. This book was released on 2019-08-08 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides information on methodologies and techniques concerning the biochemical laboratory, as well as improvements or advancements made on existing methodologies. Original methodologies for the purification of biological macromolecules and methodologies for metabolic pathways and enzyme kinetics are covered. The application of biochemical and biophysical methodologies for the structural and dynamic characterization of biological macromolecules is considered. The elaboration of automated systems for biochemical research and computer programs for the management and processing of experimental data are both reviewed. Development of instruments and equipment for biochemical research is also presented.
Download or read book Molecular Biology Techniques written by Heather Miller and published by Academic Press. This book was released on 2011-10-18 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Biochemistry Laboratory Techniques written by Sterling Chaykin and published by . This book was released on 1970 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Download or read book Modern Experimental Biochemistry written by Rodney F. Boyer and published by Pearson. This book was released on 2000 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: This successful text provides students majoring in biochemistry, chemistry, biology, and related fields with a modern and complete experience in experimental biochemistry. Its unique two-part organization offers flexibility to accommodate various requirements of the course, and allows students to reference detailed theory sections for clarification during labs. Part I, Theory and Experimental Techniques, provides in-depth theoretical discussion organized around important techniques. A valuable reference for instructors and students, it's particularly useful to instructors who prefer to use their own customized experiments. Part II, Experiments, offers optimum flexibility through 15 tested experiments designed to accommodate the capabilities of laboratories and students at most four-year schools. Alternate methods are suggested and labs may be divided into manageable hour segments.
Download or read book Basic Laboratory Methods for Biotechnology written by Lisa A. Seidman and published by CRC Press. This book was released on 2021-12-29 with total page 1210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content.
Download or read book Techniques in Free Radical Research written by A.T. Diplock and published by Elsevier. This book was released on 1991-12-11 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or "FOOTPRINTS" of radical-mediated damage. Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.
Download or read book Biochemistry Laboratory Techniques written by S. Chaykin and published by John Wiley & Sons. This book was released on 1966-01-15 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
