Download or read book Laboratory Manual For Genetic Engineering written by VENNISON, S. JOHN and published by PHI Learning Pvt. Ltd.. This book was released on 2009-01-01 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt: This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering. The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology. KEY FEATURES : Includes about 60 different experiments. Contains several figures to reinforce the understanding of the techniques discussed. Gives useful information about preparation of stock solutions, DNA/protein conversions, restriction enzymes and their recognition sequences, and so on in Appendices.
Download or read book Genetic Engineering written by Hala M. Abdelmigid and published by CreateSpace. This book was released on 2013-11 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: The primary motive for compiling and publishing this manual was to provide scientists, researchers, and students from national agricultural research systems, universities, and small private companies in developing countries, as well as advanced research institutions in the developed world, with a useful guide on the protocols currently in use in genetic engineering. This manual is intended to introduce you to some of the most widely used experimental procedures in biotechnology, including DNA isolation, manipulation, and cloning. You will also gain some familiarity with some of the types of equipment frequently used in biochemistry and molecular biology.The objective of this laboratory course is to provide you with hands-on experience in some of the basic, but essential laboratory skills required in molecular biology and biotechnology. Emphasis will be placed on understanding the concepts behind designing and implementing controlled experiments. The genetic engineering laboratory, like all laboratory courses, is an exploration of procedures. This means that, in order to get full benefit from the course, you will need to read the Manual; this reading will provide background information and an outline of the procedures to be performed. If you do not do this, you will find yourself wasting large amounts of class time, and annoying both your lab partners and your instructor. To encourage your understanding of the material, you will have problem sets that cover material related to the planned experiments. The genetic engineering laboratory is conducted as a “directed” research project. This means that although the general procedures are well established, the overall goal of each experiment is the acquisition of new information. Because of the nature of scientific research, predicting the outcome of experiments that have not previously been performed is difficult. It may therefore be necessary to design new experiments based on the results of previous ones, or to repeat experiments that yielded ambiguous results. On the other hand, if you approach the course will an open and flexible mindset, you will learn how research is performed in a genetic engineering laboratory.
Download or read book Laboratory Manual on Biotechnology written by P. M. Swamy and published by Rastogi Publications. This book was released on 2008 with total page 638 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Manipulation of Streptomyces written by D. A. Hopwood and published by . This book was released on 1985 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology Techniques written by Sue Carson and published by Academic Press. This book was released on 2019-03-05 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Manipulation and Expression of Recombinant DNA written by Sue Carson and published by Elsevier. This book was released on 2005-12-15 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Recombinant DNA Laboratory Manual written by Judith W. Zyskind and published by Elsevier. This book was released on 2014-05-12 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
Download or read book Plant Molecular Biology A Laboratory Manual written by Melody S. Clark and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Download or read book Techniques in Genetic Engineering written by Isil Aksan Kurnaz and published by CRC Press. This book was released on 2015-05-08 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although designed for undergraduates with an interest in molecular biology, biotechnology, and bioengineering, this book-Techniques in Genetic Engineering-IS NOT: a laboratory manual; nor is it a textbook on molecular biology or biochemistry. There is some basic information in the appendices about core concepts such as DNA, RNA, protein, genes, and
Download or read book CRISPR Cas written by Jennifer A. Doudna and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.
Download or read book Genetic Manipulation of Streptomyces written by David A. Hopwood and published by . This book was released on 1985-01-01 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gene Transfer written by Theodore Friedmann and published by CSHL Press. This book was released on 2007 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.
Download or read book Zero to Genetic Engineering Hero written by Justin Pahara and published by Maker Media, Inc.. This book was released on 2021-08-19 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zero to Genetic Engineering Hero is made to provide you with a first glimpse of the inner-workings of a cell. It further focuses on skill-building for genetic engineering and the Biology-as-a-Technology mindset (BAAT). This book is designed and written for hands-on learners who have little knowledge of biology or genetic engineering. This book focuses on the reader mastering the necessary skills of genetic engineering while learning about cells and how they function. The goal of this book is to take you from no prior biology and genetic engineering knowledge toward a basic understanding of how a cell functions, and how they are engineered, all while building the skills needed to do so.
Download or read book Gene Transfer and Expression written by Michael Kriegler and published by Springer. This book was released on 1990-06-18 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.
Download or read book Advanced Bacterial Genetics written by Ronald W. Davis and published by . This book was released on 1982 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Synthetic Biology A Lab Manual written by Forster Anthony C and published by World Scientific. This book was released on 2014-04-16 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
