Download or read book Isotopic Tracer Techniques in Preclinical Research written by Shanmugasundaram Ganapathy-Kanniappan and published by Springer Nature. This book was released on 2022-05-03 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book serves as a practical guide and a manual on the application of isotopic tracers in understanding the cellular processes (e.g. molecular interactions, protein synthesis) in growth, development, and disease conditions. The techniques described in the book have been carefully chosen to underscore the role and relevance of isotopic tracers in the identification of molecular targets and mechanisms, and preclinical validation of potential therapeutics. This book is intended to be a valuable resource to non-experts that may include students, researchers, educators/teachers, and others who have an interest to understand the application of isotopic tracers in research. Each chapter includes a background, an outline of the method and underlying principles, and a detailed step-wise protocol. To augment the clarity and intellectual grasp, the chapters also include relevant illustrations and study questions. More importantly, the book covers techniques involving radioactive as well as non-radioactive tracers to expand the scope of isotopes in preclinical research including basic and applied science.
Download or read book Isotopic Tracer Techniques in Preclinical Research Tracer technique in applied research written by Shanmugasundaram Ganapathy-Kanniappan and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book serves as a practical guide and a manual on the application of isotopic tracers in understanding the cellular processes (e.g. molecular interactions, protein synthesis) in growth, development, and disease conditions. The techniques described in the book have been carefully chosen to underscore the role and relevance of isotopic tracers in the identification of molecular targets and mechanisms, and preclinical validation of potential therapeutics. This book is intended to be a valuable resource to non-experts that may include students, researchers, educators/teachers, and others who have an interest to understand the application of isotopic tracers in research. Each chapter includes a background, an outline of the method and underlying principles, and a detailed step-wise protocol. To augment the clarity and intellectual grasp, the chapters also include relevant illustrations and study questions. More importantly, the book covers techniques involving radioactive as well as non-radioactive tracers to expand the scope of isotopes in preclinical research including basic and applied science.
Download or read book Isotopic Tracers in Biology written by Martin D. Kamen and published by Elsevier. This book was released on 2013-10-22 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isotopic Tracers in Biology: An Introduction to Tracer Methodology, Third Edition focuses on stable isotopes, structures, composition, and reactions of nitrogen and oxygen, and radioactive tracers. The book first takes a look at atomic nuclei, radioactivity, and the production of radioactive isotopes and radiation characteristics of tracer atoms. Topics include nuclear reactions, general properties of nuclei, radioactivity, target techniques and radiochemistry, and beta and gamma radiations. The text also discusses isotopic assay, radiation hazards, procedures related with sample preparation for radioactive assay, and combustion of labeled materials. The manuscript examines the biochemical, physiological, and medical aspects of tracer methodology, as well as biochemical applications, value of tracer methods for biology, intermediary metabolism, and applications to clinical research. The text also ponders on the isotopes of hydrogen, carbon, oxygen, nitrogen, phosphorus, and sulfur. Concerns include assay of deuterium and tritium, short-lived and heavy stable carbon, and oxygen, nitrogen, sulfur, and phosphorus isotopes. The publication is a dependable reference for readers interested in isotopic tracers.
Download or read book Tracers in Metabolic Research written by Robert R. Wolfe and published by . This book was released on 1984 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods for studying metabolism. Determination of substrate kinetics. Constant isotope infusion technique. Determination of substrate kinetics. Bolus-injection technique. Determination of substrate oxidation. Bicarbonate kinetics and natural enrichment. Priming the pool. Nonsteady state kinetics. Selection of sites for infusing isotope andj drawing samples. Specific application. Glucose metabolism. Specific application. Urea. Specific application. Fat meltabolism. Amino acids. Whole-body protein turnover. Total body water. Clinical application of stable isotopes. Analytical methods. Instrumentation. Mass spectrometry. Selected ion monitoring. Preparation of samples. Chromatography. Determination of specific radioactivity. Collection of CO2. Isotope ratio mass spectrometry techniques. Gas chromatography-mass spectrometry methods. In vivo approaches. Isotopic tracers. Advantages of stable isotopes. Biological effects. Enzymatic effects. Definition of terms. Influence of uptake on calculation of rate of appearance. Influence of pool size and complexity. Net movement of tracer and tracee. Clearance. Stable versus radioisotopes. Single-pool analysis. Bicarbonate recovery. Natural abundance of 13C. Practical consideration of performing recovery studies. Determining NaH13 CO3 infusion rate. Determining VCO2. Theory of priming dose. Priming secondary pools. Steele equation. In vivo assessment of steele equation. Glucose. Effective volume of distribution. Urea. Arterial versus central venous infusion.
Download or read book Translational Research Methods for Diabetes Obesity and Cardiometabolic Drug Development written by Andrew J. Krentz and published by Springer. This book was released on 2014-11-29 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: The world is beset by a pandemic of obesity and type 2 diabetes and the need for new drugs is startlingly clear; recent years have seen a huge increase in research activity to fill this gap. The development of new drugs for diabetes and obesity must be founded upon a sound appreciation of the pathophysiology of these common disorders. The dual defects of insulin resistance and impaired insulin secretion are fundamental to the pathogenesis and progression of obesity-associated type 2 diabetes. There is a need to explain how new drugs can counter insulin resistance and insulin deficiency to a broad range of professionals, from clinical scientists active in early (and later) phase drug development to specialist physicians and increasingly primary care doctors who must tailor drug regimens to the individual patient. Clinical research methods for measuring insulin action and insulin secretion have become well-established in proof-of-mechanism studies; however, selection of the best techniques is by no means straightforward. The purpose of the book is to aid the selection of the most appropriate techniques for assessing insulin action, insulin secretion and body composition in humans (with particular reference to new drugs) in phase 1 and 2 studies and aid the understanding of drug effects and non-drug treatment strategies on key biochemical-hormonal defects of obesity and type 2 diabetes. The book will assume a working knowledge of human physiology relating to glucose metabolism and will be of interest to biomedical scientists, pharmacologists, academics involved in metabolic research and clinicians practicing in these specialties.
Download or read book Translational Research Methods in Diabetes Obesity and Nonalcoholic Fatty Liver Disease written by Andrew J. Krentz and published by Springer. This book was released on 2019-04-02 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to aid the selection of the most appropriate methods for use in early phase (1 and 2) clinical studies of new drugs for diabetes, obesity, non-alcoholic fatty liver disease (NAFLD) and related cardiometabolic disorders. Clinical research methods to assess the pharmacokinetics and pharmacodynamics of new diabetes drugs, e.g. the euglycemic clamp technique, have become well-established in proof-of-mechanism studies. However, selection of the most appropriate techniques is by no means straightforward. Moreover, the application of such methods must conform to the regulatory requirements for new drugs. This book discusses the need for new pharmacotherapies for diabetes, obesity and NAFLD and the molecular targets of drugs currently in development. Emerging technologies including functional imaging, circulating biomarkers and omics are considered together with practical and ethical issues pertaining to early phase clinical trials in subjects with cardiometabolic disorders. Translational Research Methods in Diabetes, Obesity, and Non-Alcoholic Fatty Liver Disease is of interest to biomedical scientists, pharmacologists, academics involved in metabolic research and clinicians practicing in these specialties.
Download or read book Methods of Development of New Anticancer Drugs written by National Cancer Institute (U.S.) and published by . This book was released on 1977 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Animal Cell Technology written by Manuel J.T. Carrondo and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal cell technology has undergone a rapid transformation over the last decade from a research tool and highly specialised technology to a central resource for innovation in pharmaceutical research and development. These proceedings of the 14th Meeting of the European Society for Animal Cell Technology (Vilamoura, Portugal, May 1996) bring up to date the historical perspective of animal cell technology for the benefit of society, `From Vaccines to Genetic Medicine', and will charter this vital technology for the years to come. Strong contributions are grouped in the traditional ESACT areas of 'Cell and Physiology Engineering' dealing with cell state, including genetics, and its environment, and 'Animal Cell Process Engineering' covering integration of bioreaction with bioseparation coupled with on-line monitoring to improve protein production and consistency. Extensive coverage of metabolic engineering on synthesis, folding, assembly, transiting and secretion is dealt with in the session on 'Recombinant Proteins: Biosynthesis and Bioprocessing'. Two traditional but expanding areas of animal cell technology relevance are highlighted in the broad sessions of 'Animal Cells as Tools for Discovery and Testing' and 'Animal Cell Vaccines: Present and Future'. Two sessions finally cover the more recent domains of animal cell technology work - 'Tissue Engineering and Biomedical Devices' and 'Cells and Vectors for Genetic Medicine' - where one can foresee a very bright future.
Download or read book Biophysical Techniques in Drug Discovery written by Angeles Canales and published by Royal Society of Chemistry. This book was released on 2017-11-20 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: With perspectives from academia and industry across a spectrum of techniques, this is a go-to volume for biophysicists, analytical chemists and medicinal chemists looking for a broad overview of techniques of contemporary interest in drug discovery.
Download or read book Radiopharmaceutical Chemistry written by Jason S. Lewis and published by Springer. This book was released on 2019-04-02 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a comprehensive guide to radiopharmaceutical chemistry. The stunning clinical successes of nuclear imaging and targeted radiotherapy have resulted in rapid growth in the field of radiopharmaceutical chemistry, an essential component of nuclear medicine and radiology. However, at this point, interest in the field outpaces the academic and educational infrastructure needed to train radiopharmaceutical chemists. For example, the vast majority of texts that address radiopharmaceutical chemistry do so only peripherally, focusing instead on nuclear chemistry (i.e. nuclear reactions in reactors), heavy element radiochemistry (i.e. the decomposition of radioactive waste), or solely on the clinical applications of radiopharmaceuticals (e.g. the use of PET tracers in oncology). This text fills that gap by focusing on the chemistry of radiopharmaceuticals, with key coverage of how that knowledge translates to the development of diagnostic and therapeutic radiopharmaceuticals for the clinic. The text is divided into three overarching sections: First Principles, Radiochemistry, and Special Topics. The first is a general overview covering fundamental and broad issues like “The Production of Radionuclides” and “Basics of Radiochemistry”. The second section is the main focus of the book. In this section, each chapter’s author will delve much deeper into the subject matter, covering both well established and state-of-the-art techniques in radiopharmaceutical chemistry. This section will be divided according to radionuclide and will include chapters on radiolabeling methods using all of the common nuclides employed in radiopharmaceuticals, including four chapters on the ubiquitously used fluorine-18 and a “Best of the Rest” chapter to cover emerging radionuclides. Finally, the third section of the book is dedicated to special topics with important information for radiochemists, including “Bioconjugation Methods,” “Click Chemistry in Radiochemistry”, and “Radiochemical Instrumentation.” This is an ideal educational guide for nuclear medicine physicians, radiologists, and radiopharmaceutical chemists, as well as residents and trainees in all of these areas.
Download or read book National Cancer Institute Monograph written by and published by . This book was released on 1977 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Energy Research Abstracts written by and published by . This book was released on 1987 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Comprehensive Guide to Toxicology in Preclinical Drug Development written by Ali S. Faqi and published by Academic Press. This book was released on 2012-10-18 with total page 903 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Comprehensive Guide to Toxicology in Preclinical Drug Development is a resource for toxicologists in industry and regulatory settings, as well as directors working in contract resource organizations, who need a thorough understanding of the drug development process. Incorporating real-life case studies and examples, the book is a practical guide that outlines day-to-day activities and experiences in preclinical toxicology. This multi-contributed reference provides a detailed picture of the complex and highly interrelated activities of preclinical toxicology in both small molecules and biologics. The book discusses discovery toxicology and the international guidelines for safety evaluation, and presents traditional and nontraditional toxicology models. Chapters cover development of vaccines, oncology drugs, botanic drugs, monoclonal antibodies, and more, as well as study development and personnel, the role of imaging in preclinical evaluation, and supporting materials for IND applications. By incorporating the latest research in this area and featuring practical scenarios, this reference is a complete and actionable guide to all aspects of preclinical drug testing. - Chapters written by world-renowned contributors who are experts in their fields - Includes the latest research in preclinical drug testing and international guidelines - Covers preclinical toxicology in small molecules and biologics in one single source
Download or read book Drug Discovery and Evaluation Methods in Clinical Pharmacology written by H.Gerhard Vogel and published by Springer Science & Business Media. This book was released on 2010-12-15 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: Drug Discovery and Evaluation has become a more and more difficult, expensive and time-consuming process. The effect of a new compound has to be detected by in vitro and in vivo methods of pharmacology. The activity spectrum and the potency compared to existing drugs have to be determined. As these processes can be divided up stepwise we have designed a book series "Drug Discovery and Evaluation" in the form of a recommendation document. The methods to detect drug targets are described in the first volume of this series "Pharmacological Assays" comprising classical methods as well as new technologies. Before going to man, the most suitable compound has to be selected by pharmacokinetic studies and experiments in toxicology. These preclinical methods are described in the second volume „Safety and Pharmacokinetic Assays". Only then are first studies in human beings allowed. Special rules are established for Phase I studies. Clinical pharmacokinetics are performed in parallel with human studies on tolerability and therapeutic effects. Special studies according to various populations and different therapeutic indications are necessary. These items are covered in the third volume: „Methods in Clinical Pharmacology".
Download or read book Advancing Nuclear Medicine Through Innovation written by National Research Council and published by National Academies Press. This book was released on 2007-09-11 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nearly 20 million nuclear medicine procedures are carried out each year in the United States alone to diagnose and treat cancers, cardiovascular disease, and certain neurological disorders. Many of the advancements in nuclear medicine have been the result of research investments made during the past 50 years where these procedures are now a routine part of clinical care. Although nuclear medicine plays an important role in biomedical research and disease management, its promise is only beginning to be realized. Advancing Nuclear Medicine Through Innovation highlights the exciting emerging opportunities in nuclear medicine, which include assessing the efficacy of new drugs in development, individualizing treatment to the patient, and understanding the biology of human diseases. Health care and pharmaceutical professionals will be most interested in this book's examination of the challenges the field faces and its recommendations for ways to reduce these impediments.
Download or read book Cancer Chemotherapy Reports written by and published by . This book was released on 1968 with total page 1262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Summary Report of the Chemotherapy Program NCI 1972 written by National Cancer Institute (U.S.) and published by . This book was released on 1972 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
