Download or read book Isolation of Genes from Chronosome Region 1p31 Involved in the Development of Breast Cancer written by and published by . This book was released on 2003 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt: Loss of heterozygosity in the 1p3l region is a common event in breast cancer development suggesting the location of a tumor suppressor gene. Using a combination of molecular cloning and bioinformatics strategies, we identified 18 known genes and 16 predicted genes within the critical region of 1p31. Molecular analysis of selected genes such as TTC4 and the progesterone receptor (PTGFR) did not identify nonsense mutations in breast tumors. A comparison of expression profiles for the 18 known genes between tumor and normal tissue demonstrated seven which wee down regulated in breast cancer. No nonsense mutations were found in these genes although protein altering missense mutations were detected specifically in tumor DNA compared with the paired normal tissue. Verification of the rolf of any of these genes in breast cancer will require functional assays. During the course of these analyses, we also identified the CLCA2 gene in 1p31 which was down regulated in 50% of breast cancer and cell lines. Bisulfite secuencing showed that promoter methylation was the cause of the inactivation of this gene and treatment with 5-azacytidine could reactive it in the MDA-NB435 and MDA-MB231 cell lines. These results strongly suggest that CLCA2 could be a critical gene in 1p31 for breast cancer development.
Download or read book Isolation of Genes from Chromosome Region Ip31 Involved in the Development of Breast Cancer written by and published by . This book was released on 1999 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: Loss of heterozygosity (LOH) involving the p31 region of chromosome 1 has been reported as one of the most frequent genetic changes in human breast cancer. LOH is generally considered a mechanism for exposing recessive mutations in genes critical for tumorigenesis. We have begun creating a contiguous array of bacterial artificial chromosome (BAC) clones spanning the minimum region in Ip31 defined by LOH studies. These clones will facilitate the search for genes from the region which can be tested for mutations in breast tumors. One such gene, TTC4, has been identified, cloned and characterized. This gene contains a tetratricopeptide repeat motif which has been implicated in protein-protein binding and one member of this extended family of genes has been implicated in liver tumorigenesis.
Download or read book Obzor raboty gorodskich bibliotek written by and published by . This book was released on 1967 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation of a Breast Cancer Tumor Suppressor Gene From Chromosome 3p written by and published by . This book was released on 1996 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Loss of tumor suppressor genes (TSGs) represent critical molecular events in the development and progression of breast cancer. Based on loss of heterozygosity (LOH) studies as well as direct cytogenetic studies of breast tumors, one or more TSGs likely resides on the short arm of chromosome 3 (3p) and appears to be involved in nearly 50% of breast cancers. Four distinct regions within 3p PL2, PL4, P2L (PROXIMAL) AND P2L (DISTAL) undergo recurrent deletions in human carcinomas and are the most likely sites for a breast cancer TSG. We demonstrated recurrent homozygous deletion or rearrangement in breast cancer cell lines involving 3pl4. A set of DNA clones spanning the critical region has been sequenced revealing several potential gene coding segments, some of which we now know are expressed in breast tumors. YACs containing the critical region have been modified with the Neomycin resistance gene for selective retention in mammalian cells. Introduction of the YAC into a murine fibrosarcoma cell line appears to inhibit its tumorigenicity, supporting the hypothesis that a tumor suppressor gene resides in the critical region. Evaluation of other regions for involvement in breast cancer as originally proposed using deletion detection, DNA sequence analysis, gene isolation and expression assays, is ongoing.
Download or read book Identifying and Isolating Breast Cancer Associated Genes on Chromosomes written by and published by . This book was released on 1999 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: A region on human chromosome ii at plS.S is associated with breast cancer. Metastasis and poor outcome are the cancer phenotypes associated with this region. This is a study to identif
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1850 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cancer Research written by and published by . This book was released on 2007-02 with total page 1086 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mendelian Inheritance in Man written by Victor A. McKusick and published by JHU Press. This book was released on 1998-06-29 with total page 1728 pages. Available in PDF, EPUB and Kindle. Book excerpt: The twelfth edition of this classic reference work includes: - More than 2,000 new entries - A total of more than 9,000 entries - New features and enhancement of the familiar old features - Mapping information on more than 4,000 genes of known function - Information on specific point mutations responsible for more than 700 genetic disorders or neoplasms Mendelian Inheritance in Man (MIM) is a genetic knowledgebase that serves clinical medicine and biomedical research, including the Human Genome Project. It aims to be comprehensive (not only complete, but also collated, integrated, and interpreted), authoritative (not only accurate but also sound in its interpretations and judgements), and timely (not only up-to-date but also historically dimensioned). From a review of the eleventh edition, Reproductive Toxicology: "Even the convenience of computer-based forms of MIM cannot eliminate the need for MIM in book form. The preface provides a wonderful synopsis of human genetics. The information contained in this text serves as a concise review for those with a genetics background." From a review of the tenth edition, New England Journal of Medicine: " Victor McKusick] has been for all these years the shepherd of the development of the field of clinical genetics]. Perhaps his most important pragmatic achievement has been the 10 editions of Mendelian Inheritance in Man, which rapidly became and has remained the principal source of information on inherited diseases for all clinical geneticists. "In addition to the erudite entries in the books, the references given with each description represent a magnificent bibliography of clinical genetics. With McKusick's leadership and continued interest in gene mapping, the book also rep-resents an important compen-dium of the location of genes on specific chromosomes. "The book is a magnificent security blanket for the clinical geneticist and should be in the libraries not only of these specialists, but also of all others who see patients with diseases that have genetic components."
Download or read book Genetics Abstracts written by and published by . This book was released on 1999 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proceedings of the National Academy of Sciences of the United States of America written by National Academy of Sciences (U.S.) and published by . This book was released on 1988 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Proceedings of the National Academy of Sciences (PNAS) publishes research reports, commentaries, reviews, colloquium papers, and actions of the Academy. PNAS is a multidisciplinary journal that covers the biological, physical, and social sciences.
Download or read book Index Medicus written by and published by . This book was released on 2002 with total page 1734 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2566 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cambridge Scientific Biochemistry Abstracts written by and published by . This book was released on 1988 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Principles and Applications of Molecular Diagnostics written by Nader Rifai and published by Elsevier. This book was released on 2018-06-13 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. Explains the principles and tools of molecular biology Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results Provides a detailed description of current molecular applications used to solve diagnostics tasks
Download or read book Molecular Genetic Pathology written by Liang Cheng and published by Springer. This book was released on 2013-03-05 with total page 1136 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Genetic Pathology, Second Edition presents up-to-date material containing fundamental information relevant to the clinical practice of molecular genetic pathology. Fully updated in each area and expanded to include identification of new infectious agents (H1N1), new diagnostic biomarkers and biomarkers for targeted cancer therapy. This edition is also expanded to include the many new technologies that have become available in the past few years such as microarray (AmpliChip) and high throughput deep sequencing, which will certainly change the clinical practice of molecular genetic pathology. Part I examines the clinical aspects of molecular biology and technology, genomics. Poharmacogenomics and proteomics, while Part II covers the clinically relevant information of medical genetics, hematology, transfusion medicine, oncology, and forensic pathology. Supplemented with many useful figures and presented in a helpful bullet-point format, Molecular Genetic Pathology, Second Edition provides a unique reference for practicing pathologists, oncologists, internists, and medical genetisists. Furthermore, a book with concise overview of the field and highlights of clinical applications will certainly help those trainees, including pathology residents, genetics residents, molecular pathology fellows, internists, hematology/oncology fellows, and medical technologists in preparing for their board examination/certification.
Download or read book Gene Therapy of Cancer written by Stanton L. Gerson and published by Elsevier. This book was released on 2002-04-04 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of Gene Therapy of Cancer provides crucial updates on the basic science and ongoing research in this field, examining the state of the art technology in gene therapy and its therapeutic applications to the treatment of cancer. The clinical chapters are improved to include new areas of research and more successful trials. Chapters emphasize the scientific basis of gene therapy using immune, oncogene, antisense, pro-drug activating, and drug resistance gene targets, while other chapters discuss therapeutic approaches and clinical applications. This book is a valuable reference for anyone needing to stay abreast of the latest advances in gene therapy treatment for cancer. Provides in-depth description of targeted systems and treatment strategies Explains the underlying cancer biology necessary for understanding a given therapeutic approach Extensively covers immune therapeutics of vaccines, cytokines, and peptide-induced responses Presents translational focus with emphasis on requirements for clinical implementation Incorporates detailed illustrations of vectors and therapeutic approaches ideal for classroom presentations and general reference
Download or read book Thiol Redox Transitions in Cell Signaling Part B written by and published by Academic Press. This book was released on 2010-08-19 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
