Download or read book Isolation and Genetic Characterization of a Temperature sensitive Mutant of Escherichia Coli Defective in the Modification of 50S Ribosomal Proteins written by Valerie Maples and published by . This book was released on 1975 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Characterization of Temperature sensitive Protein Synthesis Mutants of Escherichia Coli by Directed Mutagenesis of the Defective Bacteriophage Lambda Fus2 written by Kenton Lee Lohman and published by . This book was released on 1985 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cold sensitive Mutants of Escherichia Coli Defective in Ribosome Assembly written by Christine Guthrie and published by . This book was released on 1970 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Characterization of a Temperature sensitive Mutation in the 88 89 Minutes of the Escherichia Coli Chromosome written by Issmat Ibrahim Kassem and published by . This book was released on 2003 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: In an attempt to isolate a temperature sensitive allele of ftsN, chemical random mutagenesis was performed using hydroxylamine. SMM 12 a temperature sensitive filamentous strain was successfully isolated. However, it did not carry an ftsN(Ts) allele. Transforming with different pasmids carrying cloned genes and random genomic plasmid libraries have yielded no complementation. Using P I -mediated transduction, the location of the mutation was found to be at the 88-89 minute region on the E. coli chromosome. This finding represents the discovery of an unprecedented gene at the 88- 89 minute region that blocks cell division. in addition, we report in this work the isolation of the first temperature-sensitive mutant that is suppressed by L-glutamate. Although the precise identity of the mutated gene remains illusive, all data produced by this work point out that we may have isolated a previously unknown player in the cell- division protein machinery in E. coli.
Download or read book Isolation and Partial Characterization of a Temperature sensitive Escherichia Coli Mutant with Altered Leucyl transfer Ribonucleic Acid Synthetase written by Bahram Moezzi Ghajar and published by . This book was released on 1972 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Two Temperature sensitive Mutants of Escherichia Coli Exhibiting an Altered L22 Ribosomal Protein written by Bonnie A. Burnette-Vick and published by . This book was released on 1991 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Genetic Characterization of Aminotriazole Resistant Temperature sensitive Mutants of Escherichia Coli microform written by William Mark.* Toone and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1991 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1994 with total page 1364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Genetic Characterization of Aminotriazole resistant Temperature sensitive Mutants of Escherichia Coli written by William Mark.* Toone and published by . This book was released on 1991 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Characterization of a Temperature sensitive Strain of Escherichia Coli written by William Harley Schubach and published by . This book was released on 1971 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Eight Temperature Sensitive Mutants of Escherichia Coli by Genetic Analysis and Molecular Cloning written by Katherine Armstrong and published by . This book was released on 1981 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Isolation and Partial Characterization of a Temperature Sensitive Mutant of E coli K12 Defective in Oxidative Phosphorylation written by Gila Shtrauch and published by . This book was released on 1974 with total page 27 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Suppression of Filamentation in Escherichia Coli Mutant SM6716 by Overexpression of RNA Polymerase Beta and Ribosomal Protein L7 12 written by Rima Ghassan Alaouie and published by . This book was released on 2006 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt: Strain SM6716 is a division mutant of Escherichia coli with a temperature- sensi tive phenotype and previously isolated using P1-mediated hydroxylamine mutagenes is. Strain SM6716 was suspected to carry a mutation in the essential cell divisi on gene ftsN. However, transformation with plasmids carrying wild-type ftsN fail ed to complement the mutation, and chromosomal mapping by P1-transduction showed that the mutation is located in 89-90 minute region on the chromosome of E. col i. Using a pBR325-based plasmid library of HindIII-generated fragments of the E. coli genome, we obtained a clone with a ̃10Kbp HindIII fragment that was able t o complement the temperature-sensitive mutation in SM6716. Further restriction a nd deletion analysis of the complementing plasmid (pR10) demonstrated that the c o-overexpression of the rplL and rpoB genes, encoding the L7/L12 protein of the 50S subunit of the E. coli ribosome and the beta subunit of the RNA polymerase, respectively, was required for complementation. The results suggest strongly tha t the mutation responsible for the temperature-sensitive phenotype in strain SM6 716 resides in and affects expression from an upstream promoter (rplJp), resulti ng in under-expression of rplL and rpoB and leading to failure in cell division at 42 degrees C. Further characterization of the mutation in SM6716 should lead to the identification of the mutation site, and to proposing a mechanism by whi ch transcription, ribosomal function and cell division in Escherichia coli are r elated.
Download or read book Isolation and characterization of dnaX and dnaY temperature sensitive mutants of Escherichia coli written by Joan Marie Henson and published by . This book was released on 1979 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Genetic Analysis of Ribosomal Proteins in Escherichia Coli written by Janice Marie Zengel and published by . This book was released on 1976 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proceedings of the National Academy of Sciences of the United States of America written by National Academy of Sciences (U.S.) and published by . This book was released on 1977-12 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Genetic Characterization of a New Mutation in Escherichia Coli Requiring Functional RecA Protein for Viability written by Jonathan Clyman and published by . This book was released on 1984 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
