Download or read book Isolation and Characterization of Polychlorinated Biphenyl PCB Degrading Bacteria Isolated from a PCB Contaminated Reservoir in Donna TX written by and published by . This book was released on 2014 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Metabolic and Phenotypic Characterization of Aerobic PCB Degrading Bacteria Isolated from Kalamazoo River Sediments written by Brenton K. Lehmkuhl and published by . This book was released on 2006 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Kalamazoo River is contaminated with polychlorinated biphenyls (PCBs) which are neurotoxins and suspected carcinogens. The goal of this study was to isolate, identify, and metabolically characterize PCB degrading strains of aerobic bacteria. Biphenyl enrichments resulted in the isolation of putative PCB degrading strains from different locations along the river. Based on 16S rRNA gene analysis the isolated strains were identified as belonging to the genera of Achromobacter, Acidovorax, Cellulosimicrobium, Paenibacillus, Pseudomonas, and Rhodococcus. These strains were screened for the PCB degrading ability and congener specificity. All of the isolated strains degraded PCBs via the 2,3-dioxygenase pathway. The range of PCBs degraded was limited to mono- to tri-substituted congeners. In addition, the isolated strains oxidized 2,4'-CB but accumulated problematic intermediate metabolites. These results indicate that most of the aerobic-PCB-degrading bacteria isolated in this study from the Kalamazoo River have a moderate potential to degrade the lesser chlorinated PCBs, while one strain (D1) was found to have above average PCB-degrading capabilities.
Download or read book Isolation and Characterization of Bacteria for Polychlorinated Biphenyl Degradation written by and published by . This book was released on 1992 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Identification of Biphenyl and Polychlorinated Biphenyl Degrading Microbes written by Hasan Hasnaeen Ahmed and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The San Jacinto River system south of the I-10 overpass is heavily contaminated with biphenyl and polychlorinated biphenyls (PCBs), carcinogenic aromatic hydrocarbons that have a deleterious impact on the environment. While typically recalcitrant to most forms of environmental degradation, biphenyl and PCBs compounds are known to share a common microbial degradation pathway with other chlorinated aromatics such as polychlorinated dioxin (PCDD) and dibenzofuran (PCDF). As such the purpose of this research is to isolate and test bacterial strains collected from contaminated San Jacinto River sediment and soil for the capacity to degrade or fully metabolize biphenyl and its derivatives. As conventional clean-up is expensive, such strains can be used in the field of bioremediation as a more cost-effective way of treating environmental contamination. The samples were screened for six weeks in minimal media with putative positive samples isolated for further bioinformatics analysis. Microorganisms identified as Pseudomonas pseudoalcaligenes, Pseudomonas nitroreducens, and Comamonas sp. were noted to harbor a putative 2,3 biphenyl dioxygenase enzyme and were further assessed for possible degradative capacity through a combination of growth kinetics and GC-MS analysis to determine the quality and quantity of the intermediate metabolites. Sample growth results suggest that each of the three isolated study strains are unable to utilize biphenyl or 3-chlorobiphenyl as a sole carbon source. In addition, expected metabolites of biphenyl degradation were not observed in either the supernatant or cell lysate indicating that expression of these putative 2,3 biphenyl dioxygenase enzymes and/or their catalytic activity against biphenyl is likely to be minimal.
Download or read book Characterization of Soil Bacterial Communities Involved in Polychlorinated Biphenyl Remediation written by Ekawan Luepromchai and published by . This book was released on 2001 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Physiological Characterization of Two Chlorobenzoic Acid Degrading Bacteria from Polychlorinated Biphenyl Contaminated Soils written by Carlos Barreno Miguez and published by . This book was released on 1993 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Uptake of benzoic acid by BRI 3010 and BRI 6011 was inducible, exhibited saturation kinetics and the substrate was accumulated intracellularly against a concentration gradient by a factor of 8 and 10, respectively, indicative of active transport. Uptake of 2,4-DCBA by BRI 6011 was constitutive and saturation kinetics were not observed, suggesting passive diffusion of 2,4-DCBA and other CBAs into the cell down a concentration gradient." --
Download or read book PCB in Water written by Water Resources Scientific Information Center and published by . This book was released on 1973 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Determination of Polychlorinated Biphenyls PCBs in Sediment and Biota written by Lynda Webster and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Screening of a Polychlorinated Biphenyl PCB Degrading Organism and the Measurement of Its PCB Degrading Capability by the Use of an Immunoassay System written by Alfredo I. Chandia and published by . This book was released on 1999 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: An organism with PCB-degrading capabilities was isolated from a site located in Eagle Lake, Minnesota, and its PB-degrading competence was measured. Study confirms that there are naturally occurring microorganisms in soil with the ability to degrade man-made compounds.
Download or read book Microbial Dechlorination of PCBs in Vitro Studies Using an Anaerobic Environmental Isolate Final Report written by and published by . This book was released on 1990 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt: An earlier progress report, prepared in September 1990, gave an account of the scientific concepts on which this research project is based. It described the design and initial results of an experiment that was carried out to examine this important scientific question. The question, on which scientific and technical personnel of the Oak Ridge Research Institute (ORRI) and the Oak Ridge National Laboratory (ORNL) have been collaborating, is whether there are microbial isolates whose metabolism may be harnessed for the biodegradation of complex organic molecules containing chlorine, such as PCBs. These compounds have been shown to have a persistent long-term presence in the environment and to represent a health hazard to the populations. The demonstration that certain strains of microorganisms have the capability to degrade these complex organic molecules would be important because of the difficulty of remediating PCB-polluted environmental sites by other noninvasive methods. The adaptability of microbial metabolism is key to the concept that bacteria isolated from contaminated sites may be the most likely candidates for strains that could have developed the capability to either tolerate or possibly metabolize the organic contaminants. Consequently, a number of bacterial strains have been isolated from PCB-contaminated sites on the Department of Energy (DOE) reservation and screened for possible PCB dechlorination activity. The subject of the experiment described herein is an isolate designated ES-3 (an obligate anaerobe isolated from a PCB-contaminated site), which is considered on initial screening to possess possible PCB dechlorination ability. The experiment described in this report involved the incubation of aliquots of this isolate in an anaerobic environment for up to eight weeks with various concentrations, separately, of either of the Arochlors 1016 or 1260.
Download or read book Biostimulation of Polychlorinated Biphenyl pcb Dechlorination in Sediments of Escambia Bay Florida written by Brittany Elise Young and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Polychlorinated biphenyls (PCBs) contaminate the sediments of Escambia Bay in Pensacola, Florida. Given the right conditions anaerobic bacteria in sediments are capable of degrading PCBs by dechlorinating the molecule. The objective of this study is to assess, in the laboratory, conditions that may stimulate the naturally occurring bacteria in Escambia Bay to degrade the PCBs. The study may have implications for in situ treatment of contaminated sediments like these in Escambia Bay. Sediments were treated with three different electron donors (pyruvate, iron sulfide and elemental iron) that would stimulate the microbes to use the PCBs as an electron acceptor. Each treatment was tested in triplicate and allowed to dechlorinate for five months in an anaerobic environment. After five months, all three treatments resulted in a decrease in PCB concentration in sediment spiked with 2,3'4,4'5-pentachlorobiphenyl (congener 118) and in sediment spiked with Aroclor 1254. Unspiked PCB contaminated sediments showed a less consistent decrease in total PCB concentration for the various treatments. These results show that the bacteria found in Escambia Bay sediments can be stimulated to remediate the PCB contamination. Further research could develop this method into a viable in situ remediation strategy to address the contamination in Escambia Bay.
Download or read book Characterizing Polychlorinated Biphenyl Degradation Potential in Surface Water Bacteria from Rio Grande Valley Texas Reservoirs written by Alisha M. Janiga and published by . This book was released on 2018 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Source Identification and Fish Exposure for Polychlorinated Biphenyls Using Congener Analysis from Passive Water Samplers in the Millers River Basin Massachusetts written by John A. Colman and published by . This book was released on 2001 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: ... Reports results of a USGS study to determine source(s) of elevated PCB concentration observed in fish and to establish the extent of fish exposure to PCB's along the entire main stems of the Millers River and one of it's tributaries, the Otter River ...
Download or read book Response of Sediment Bacterial Communities to Polychlorinated Biphenyl PCB Contamination in the Upper Hudson River New York written by Bridget Lee Menasche and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Partial Characterization of PCB and PAH degrading Bacterial Consortia from Contaminated Sites in Stephenville and Argentia Island of Newfoundland written by Deborah V. Squires-Parsons and published by . This book was released on 2005 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of PCB polychlorobiphenyl Dechlorination by Anaerobic Microorganisms from Hudson River Sediments written by Dingyi Ye and published by . This book was released on 1994 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation of Polychlorinated Biphenyl Degrading Microorganisms from Puget Sound Washington written by Sheryl Everson Dyksterhouse and published by . This book was released on 1992 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt:
