Download or read book Isolation and Characterization of a Temperature sensitive Strain of Escherichia Coli written by William Harley Schubach and published by . This book was released on 1971 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Characterization of a Temperature sensitive Mutation in the 88 89 Minutes of the Escherichia Coli Chromosome written by Issmat Ibrahim Kassem and published by . This book was released on 2003 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: In an attempt to isolate a temperature sensitive allele of ftsN, chemical random mutagenesis was performed using hydroxylamine. SMM 12 a temperature sensitive filamentous strain was successfully isolated. However, it did not carry an ftsN(Ts) allele. Transforming with different pasmids carrying cloned genes and random genomic plasmid libraries have yielded no complementation. Using P I -mediated transduction, the location of the mutation was found to be at the 88-89 minute region on the E. coli chromosome. This finding represents the discovery of an unprecedented gene at the 88- 89 minute region that blocks cell division. in addition, we report in this work the isolation of the first temperature-sensitive mutant that is suppressed by L-glutamate. Although the precise identity of the mutated gene remains illusive, all data produced by this work point out that we may have isolated a previously unknown player in the cell- division protein machinery in E. coli.

Download or read book Isolation and Partial Characterization of a Temperature sensitive Escherichia Coli Mutant with Altered Leucyl transfer Ribonucleic Acid Synthetase written by Bahram Moezzi Ghajar and published by . This book was released on 1972 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and characterization of dnaX and dnaY temperature sensitive mutants of Escherichia coli written by Joan Marie Henson and published by . This book was released on 1979 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Genetic Characterization of a Temperature sensitive Mutant of Escherichia Coli Defective in the Modification of 50S Ribosomal Proteins written by Valerie Maples and published by . This book was released on 1975 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Isolation and Partial Characterization of Some Mutants of Escherichia Coli K 12 with Temperature sensitive Synthesis of DNA written by Philip Louis Carl and published by . This book was released on 1968 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Genetic Characterization of Aminotriazole Resistant Temperature sensitive Mutants of Escherichia Coli microform written by William Mark.* Toone and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1991 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Genetic Characterization of Aminotriazole resistant Temperature sensitive Mutants of Escherichia Coli written by William Mark.* Toone and published by . This book was released on 1991 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Characterization of Temperature sensitive Protein Synthesis Mutants of Escherichia Coli by Directed Mutagenesis of the Defective Bacteriophage Lambda Fus2 written by Kenton Lee Lohman and published by . This book was released on 1985 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Preliminary Characterization of a New Temperature sensitive Allele of the NAD dependent Ligase Gene ligA in Escherichia Coli written by Hadi Mohamad Yassine and published by . This book was released on 2003 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Temperature-sensitive (Ts) mutants are considered a valuable tool to study the functional properties of many essential proteins in Escherichia coli. These include protein-protein interactions, DNA-protein interactions, protein localization and the characterization of functional domains in proteins. Using P I-mediated localized mutagenesis, we were able to generate a number of Ts mutants by targeting genes at the 54.4 minutes on Escherichia coli chromosome. One of which, termed LS3533 was isolated for further testing. This 54.4 minutes locus on the Escherichia coli chromosome carries two essential genes that are involved in cell cycle. The genes are: zipA (a cell division gene) and ligA (encoding DNA ligase gene). Characterization of the mutant gene was carried out by two steps. First, through mapping the mutation using P-I transductions and second by complementing the Ts phenotype (observed at 42 C) through transforming the mutant strain with a plasmid that carries a wild type copy of the affected gene. Parallel to the identification process, the isolated strain was subjected to several physiological studies including phenotypic observation, chromosome patterns visualization, FtsZ localization and SOS response testing. Our findings demonstrated that the temperature-sensitive phenotype in isolate Ls3533 was due to a mutation in the ligA gene. The block to division at 42 C in this strain was shown to be a consequence of the induction of the SOS-response in a lexA-dependent but sfiA-independent fashion, in which the localization of the essential division protein FtsZ, to the division site is prohibited.

Download or read book Isolation and Characterization of PH Sensitive Mutants of Escherichia Coli Using Nitrosoguanidine Mutagenesis written by Ronald Scott Hutchison and published by . This book was released on 1987 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Eight Temperature Sensitive Mutants of Escherichia Coli by Genetic Analysis and Molecular Cloning written by Katherine Armstrong and published by . This book was released on 1981 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bacteriology Isolation and Characterization of Escherichia Coli Strains which are Sensitive to Hydrophilic And or Charged Antibiotics and Toxics written by B. Lambert and published by . This book was released on 1982 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Selection and Characterization of Temperature Sensitive Mutants of Escherichia Coli microform written by Roback, Ellen R and published by National Library of Canada. This book was released on 1971 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Characterization of Temperature sensitive Protein Sysnthesis Mutnats of Escherichia Coli by Directed Mutagensis of the Defective Bacteriophage Lambda FUS2 written by Kenton Lee Lohman and published by . This book was released on 1985 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of an E Coli Mutant Temperature Sensitive for RRNA Synthesis written by Jean Shearin Harris and published by . This book was released on 1976 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Two Temperature sensitive Mutants of Escherichia Coli Exhibiting an Altered L22 Ribosomal Protein written by Bonnie A. Burnette-Vick and published by . This book was released on 1991 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: