Download or read book Isoelectric Focusing Theory Methodology and Application written by Pier Giorgio Righetti and published by Elsevier. This book was released on 2000-04-01 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with theoretical and practical developments of IEF and offers detailed methodology for many of the commonly used procedures, such as IEF in gels. It is intended both as a reference guide and a practical manual.
Download or read book Laboratory techniques in biochemistry and molecular biology written by and published by Elsevier. This book was released on 1983 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isoelectric Focusing written by P. G. Righetti and published by . This book was released on 1983 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isoelectric Focusing written by Nicholas Catsimpoolas and published by Elsevier. This book was released on 2012-12-02 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isoelectric Focusing deals with the theory and applications of isoelectric focusing. The book reviews some alternative approaches to generation of pH gradients for isoelectric focusing analysis, along with other relevant topics such as the theoretical aspects of steady-state isoelectric focusing, gel electrofocusing/gel electrophoresis, and transient state isoelectric focusing. This volume is organized into nine chapters and begins by looking at the history of the discovery of isoelectric focusing and correcting some blurred impressions conveyed in the literature. It then turns to a discussion of the differential equation of isoelectric focusing, the carrier ampholytes used in carrying out the technique, the use of isoelectric focusing in polyacrylamide and granulated gels as well as in free solution, and continuous-flow electrophoresis and isoelectric focusing. An attempt is made to illuminate problems in isoelectric focusing and to point out possible solutions in the areas of theory, mechanisms, ampholyte chemistry, and apparatus. Some of the processes that occur during an isoelectric focusing experiment are also considered. This book is intended for anyone who needs to know what isoelectric focusing is all about.
Download or read book Guide to Protein Purification written by Murray P. Deutscher and published by Gulf Professional Publishing. This book was released on 1990 with total page 936 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.
Download or read book Immobilized PH Gradients Theory and Methodology written by P. G. Righetti and published by Elsevier. This book was released on 1990 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc.The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.
Download or read book Handbook of Isoelectric Focusing and Proteomics written by David Garfin and published by Elsevier. This book was released on 2005-09-29 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics.* Is the only current book available on the subject * Includes author insights and practical tips* Is an ideal single source for students and researchers working in proteomics
Download or read book Biochemistry and Molecular Biology Compendium written by Roger L. Lundblad and published by CRC Press. This book was released on 2019-11-11 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an accessible resource offering practical information not found in more database-oriented resources. The first chapter lists acronyms with definitions, and a glossary of terms and subjects used in biochemistry, molecular biology, biotechnology, proteomics, genomics, and systems biology. There follows chapters on chemicals employed in biochemistry and molecular biology, complete with properties and structure drawings. Researchers will find this book to be a valuable tool that will save them time, as well as provide essential links to the roots of their science. Key selling features: Contains an extensive list of commonly used acronyms with definitions Offers a highly readable glossary for systems and techniques Provides comprehensive information for the validation of biotechnology assays and manufacturing processes Includes a list of Log P values, water solubility, and molecular weight for selected chemicals Gives a detailed listing of protease inhibitors and cocktails, as well as a list of buffers
Download or read book Capillary Electromigration Separation Methods written by Colin F. Poole and published by Elsevier. This book was released on 2018-04-13 with total page 628 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins. - Edited and authored by world-leading capillary electrophoresis experts - Presents comprehensive coverage on the subject - Includes extensive referencing that facilitates the identification of key research developments - Provides more than 50 figures and tables that aid in the retention of key concepts
Download or read book Encyclopedia of Analytical Science written by and published by Elsevier. This book was released on 2019-04-02 with total page 5142 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of the Encyclopedia of Analytical Science, Ten Volume Set is a definitive collection of articles covering the latest technologies in application areas such as medicine, environmental science, food science and geology. Meticulously organized, clearly written and fully interdisciplinary, the Encyclopedia of Analytical Science, Ten Volume Set provides foundational knowledge across the scope of modern analytical chemistry, linking fundamental topics with the latest methodologies. Articles will cover three broad areas: analytical techniques (e.g., mass spectrometry, liquid chromatography, atomic spectrometry); areas of application (e.g., forensic, environmental and clinical); and analytes (e.g., arsenic, nucleic acids and polycyclic aromatic hydrocarbons), providing a one-stop resource for analytical scientists. Offers readers a one-stop resource with access to information across the entire scope of modern analytical science Presents articles split into three broad areas: analytical techniques, areas of application and and analytes, creating an ideal resource for students, researchers and professionals Provides concise and accessible information that is ideal for non-specialists and readers from undergraduate levels and higher
Download or read book Electrophoresis in Practice written by Reiner Westermeier and published by John Wiley & Sons. This book was released on 2016-03-16 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis. The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.
Download or read book Encyclopedia of Chromatography written by Jack Cazes and published by CRC Press. This book was released on 2009-10-12 with total page 2968 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thoroughly revised and expanded, this third edition offers illustrative tables and figures to clarify technical points in the articles and provides a valuable, reader-friendly reference for all those who employ chromatographic methods for analysis of complex mixtures of substances. An authoritative source of information, this introductory guide to specific chromatographic techniques and theory discusses the relevant science and technology, offering key references for analyzing specific chemicals and applications in industry and focusing on emerging technologies and uses.
Download or read book Proceedings of the International Symposium on the Forensic Applications of Electrophoresis written by and published by Forensic Science Research and Training Center FBI Academy. This book was released on 1985 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Purification Protocols written by Paul Cutler and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Download or read book Isoelectric Focusing Theorie Methodology and Applications written by Pier Giorgio Righetti and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Theoretical Advancement in Chromatography and Related Separation Techniques written by Francesco Dondi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 651 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography and all the related separation techniques are experimental in their origin and justification. However, the spectacular progress made in this area since World War II has given rise to a theoretical underpinning. The present book covers the current status of the research area and places it in perspective with the general concepts of the fields of physical chemistry involved. The ASI lectures/authors -- well known leaders in their fields -- have written presentations at the graduate level, accessible to all those who have a good general background in the thermodynamics and mass transfer theory of phase equilibria. The book will be useful to young scientists and engineers who wish to access the current frontiers in chromatography and other separation sciences.
Download or read book Capillary Electrophoresis written by Patrick Camilleri and published by CRC Press. This book was released on 1997-11-25 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.
