Download or read book Investigations in Integrative and Molecular Bioscience written by John Andrew Hawkins and published by . This book was released on 2018 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern biology is going through a revolution of new methods and insights resulting from the new availability of high-throughput DNA sequencing technology. I here present work contributing mathematical and computational methods for gaining insight from large DNA sequencing data sets at three distinct levels. First, I present a method for improving the accuracy and efficiency of DNA barcodes, short sequences of DNA used to label individual molecules in pooled samples. Many DNA sequencing applications depend on the use of DNA barcodes. However, errors in DNA synthesis and sequencing--substitutions, insertions, and deletions--confound the correct interpretation of these barcodes. I here present Filled/truncated Right End Edit (FREE) barcodes designed for barcode error-correction in the context of a downstream sequence. Second, I present the Chip-Hybridized Affinity Mapping Platform (CHAMP), a novel technology for repurposing used DNA sequencing chips to study the mechanism and sequence preferences of DNA-binding proteins. Since 2012, the CRISPR family of proteins have gained wide application for their efficiency and ease of use in editing genomes in vivo. Using CHAMP, I, in collaboration with experimentalists in Ilya Finkelstein's lab, investigated the mechanism and sequence preference of the CRISPR Cascade complex, and discovered a novel periodic lack of sequence specificity in DNA binding. I further determined specific nucleotides important for recruitment of and processing by the nuclease domain, Cas3. Third, I present a meta-analysis of the order Chiroptera, the order of bats, using the new wealth of DNA sequence information of eighteen bat species. The transcriptome sequencing data for two of these bats--Hypsignathus monstrosus and Rousettus aegyptiacus, bats associated with studies of the Ebola and Marburg viruses respectively-- is novel to this study. Using all this DNA sequence information, I reconstructed a high- confidence Chiropteran phylogeny and found 299 genes with signatures of positive selection, a signature associated with viral antagonism. Further study of these genes may shed light on the mechanism through which several bat viruses relevant to human health hijack the cell, including SARS, Ebola, Hendra, and Nipah

Download or read book Integrated Molecular and Cellular Biophysics written by Valerica Raicu and published by Springer Science & Business Media. This book was released on 2008-06-17 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics represents perhaps one of the best examples of interdisciplinary research areas, where concepts and methods from disciplines such as physics, biology, b- chemistry, colloid chemistry, and physiology are integrated. It is by no means a new ?eld of study and has actually been around, initially as quantitative physiology and partly as colloid science, for over a hundred years. For a long time, biophysics has been taught and practiced as a research discipline mostly in medical schools and life sciences departments, and excellent biophysics textbooks have been published that are targeted at a biologically literate audience. With a few exceptions, it is only relatively recently that biophysics has started to be recognized as a physical science and integrated into physics departments’ curr- ula, sometimes under the new name of biological physics. In this period of cryst- lization and possible rede?nition of biophysics, there still exists some uncertainty as to what biophysics might actually represent. A particular tendency among phy- cists is to associate biophysics research with the development of powerful new te- niques that should eventually be used not by physicists to study physical processes in living matter, but by biologists in their biological investigations. There is value in that judgment, and excellent books have been published that introduce the int- ested reader to the use of physical principles for the development of new methods of investigation in life sciences.

Download or read book Integrative Omics written by Manish Kumar Gupta and published by Elsevier. This book was released on 2024-05-10 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Integrative Omics: Concepts, Methodology and Applications provides a holistic and integrated view of defining and applying network approaches, integrative tools, and methods to solve problems for the rationalization of genotype to phenotype relationships. The reference includes a range of chapters in a systemic ‘step by step’ manner, which begins with the basic concepts from Omic to Multi Integrative Omics approaches, followed by their full range of approaches, applications, emerging trends, and future trends. All key areas of Omics are covered including biological databases, sequence alignment, pharmacogenomics, nutrigenomics and microbial omics, integrated omics for Food Science and Identification of genes associated with disease, clinical data integration and data warehousing, translational omics as well as omics technology policy and society research. Integrative Omics: Concepts, Methodology and Applications highlights the recent concepts, methodologies, advancements in technologies and is also well-suited for researchers from both academic and industry background, undergraduate and graduate students who are mainly working in the area of computational systems biology, integrative omics and translational science. The book bridges the gap between biological sciences, physical sciences, computer science, statistics, data science, information technology and mathematics by presenting content specifically dedicated to mathematical models of biological systems. Provides a holistic, integrated view of a defining and applying network approach, integrative tools, and methods to solve problems for rationalization of genotype to phenotype relationships Offers an interdisciplinary approach to Databases, data analytics techniques, biological tools, network construction, analysis, modeling, prediction and simulation of biological systems leading to ‘translational research’, i.e., drug discovery, drug target prediction, and precision medicine Covers worldwide methods, concepts, databases, and tools used in the construction of integrated pathways

Download or read book Integrative Physiology in the Proteomics and Post Genomics Age written by Wolfgang Walz and published by Springer Science & Business Media. This book was released on 2005-03-22 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advent of molecular techniques has shifted the focus of physiology from its traditional role as an integrative science concerned with the study of regulatory mechanisms leading to adaptation and homeostasis, to a field preoccupied with the problems and challenges inherent in those techniques. In Integrative Physiology in the Proteomics and Post-Genomics Age, internationally recognized researchers highlight the major questions and accomplishments of modern physiological research and demonstrate that modern molecular methods can well be incorporated and strengthen the original integrative perspectives of physiology set out by Claude Bernard's concept of the "milieu interieur." Among the critical issues discussed are the place of functional genomics in regulatory physiology, the role of model systems in integrative physiology, the function of neural circuits in behavior and consciousness, and the influence of external challenges to the whole body and the environment on genes. The question of integrative physiology in curriculum design for the health sciences is also discussed. Perceptive and timely, Integrative Physiology in the Proteomics and Post-Genomics Age bridges the gap between molecular biology and whole body function, establishing the future of physiology as an integrative science based on new molecular insights.

Download or read book Integrated Molecular Evolution written by Scott Orland Rogers and published by CRC Press. This book was released on 2011-07-27 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular evolution, phylogenetics, genomics, and other related topics are all critical to understanding evolutionary processes. All too frequently, however, they are treated separately in textbooks and courses, such that students fail to connect all of the concepts, principles, and nuances of the evolutionary processes. Integrated Molecular Evolut

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Leland J. Cseke and published by CRC Press. This book was released on 2016-04-19 with total page 735 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology

Download or read book Principles of Molecular Regulation written by P. Michael Conn and published by Springer Science & Business Media. This book was released on 2000-07-28 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the dramatic growth in our knowledge of hormone action and the mechanisms of cell regulation, the need for an up-to-date broad-ranging survey of these processes has become pressing. In Principles of Molecular Regulation, P. Michael Conn and Anthony Means have successfully assembled a panel of leading investigators to provide an integrated review of the key areas-membrane receptor-initiated cell signaling and nuclear receptor-initiated gene regulation. Chosen for the excellence of their research as well as their demonstrated writing skills, these distinguished authors illuminate the molecular machinery underlying the regulatory processes of cells. In addition to their comprehensive review of the signaling mechanisms involving cell surface receptors, nuclear receptors, and ion channels, they detail the roles of calcium, lipids, cyclic AMP, protein kinases, and protein phosphatases. They also discuss the molecular regulation of cell proliferation and death, as well as the impact of new technologies on rational drug discovery. Capturing the excitement now present at this vibrant union of molecular biology, cell biology, and endocrinology, Principles of Molecular Regulation constitutes a major new resource for understanding the many and complex elements of biological regulation. Its up-to-date critical synthesis is certain to prove of high value to all basic and clinical investigators working with these processes today.

Download or read book Molecular Biology of B Cells written by Tasuku Honjo and published by Elsevier. This book was released on 2024-01-15 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology of B Cells, Third Edition is a comprehensive reference to how B cells are generated, selected, activated, and engaged in antibody production. These developmental and stimulatory processes are described in molecular, immunological, and genetic terms to give a clear understanding of complex phenotypes. Molecular Biology of B Cells, Third Edition offers an integrated view of all aspects of B cells to produce a normal immune response as a constant, and the molecular basis of numerous diseases due to B cell abnormality. The new edition continues its success with updated research on B cell development and function, the use of therapeutic antibodies in cancer and infectious disease, therapeutic targeting of B cells for clinical application, new developments in lymphoma biology. With updated research and continued comprehensive coverage of all aspects of B cell biology, Molecular Biology of B Cells, Third Edition is the definitive resource, vital for researchers across molecular biology, immunology, and genetics. Provides new research on normal versus abnormal B cell development and function Contains studies on therapeutic antibodies in cancer and infectious diseases Covers research on therapeutically targeting B cells in inflammation or autoimmune diseases

Download or read book Biology of T Cells written by and published by Academic Press. This book was released on 2018-09-24 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology of T Cells: Part A, Volume 341, the latest release in the International Review of Cell and Molecular Biology, reviews and details current advances in cell and molecular biology. The IRCMB series maintains the highest standard by publishing timely topics authored by prominent cell and molecular biologists. Specialized topics in this release include TCR signaling: Molecules and mechanisms, TCR diversity: Purpose and generation, Transcriptional programs underlying T-cell differentiation and function, Surface phenotypes of CD8+ and CD4+ T cells, Co-stimulation and co-inhibition in CD8+ and CD4+ T cells, Regulated cell death and T cells, Molecular mechanisms behind T-cell priming by DCs, and more. Publishes only invited review articles on selected topics Authored by established and active cell and molecular biologists and drawn from international sources Offers a wide range of perspectives on specific subjects

Download or read book Integrative Approaches to Molecular Biology written by Julio Collado-Vides and published by MIT Press. This book was released on 1996 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: It charts the course of the emerging discipline of integrative molecular biology from macromolecular sequences to a biological (and theoretical) perspective, showing that novel integrative methodologies and paradigms are emerging at the confluence of such disciplines as computer science, logic, linguistics, and mathematics.

Download or read book Integrated Molecular Evolution written by Scott O. Rogers and published by CRC Press. This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Section VI: Genomes -- 27. RNA Viruses -- 28. DNA Viruses -- 29. Bacteria and Archaea -- 30. Mutualists and Pathogens -- 31. Endosymbionts and Organelles -- 32. Protein Trafficking -- 33. Eukaryotic Genomes -- 34. Human Genome -- Back Cover.

Download or read book Biochemistry written by John T. Tansey and published by Wiley Global Education. This book was released on 2019-10-28 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry addresses the diverse needs of premed, biochemistry, and life science majors by presenting relevant material while still preserving a chemical perspective. Presented within the next generation of WileyPLUS, Biochemistry emphasizes worked problems through video walkthroughs, interactive elements and expanded end-of-chapter problems with a wide range of subject matter and difficulty. The worked problems in the course are both qualitative and quantitative and model for students the biochemical reasoning they need to practice. Students will often be asked to analyze data and make critical assessments of experiments.

Download or read book Molecular and Cell Biology of Cancer written by Rita Fior and published by Springer. This book was released on 2019-06-27 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook takes you on a journey to the basic concepts of cancer biology. It combines developmental, evolutionary and cell biology perspectives, to then wrap-up with an integrated clinical approach. The book starts with an introductory chapter, looking at cancer in a nut shell. The subsequent chapters are detailed and the idea of cancer as a mass of somatic cells undergoing a micro-evolutionary Darwinian process is explored. Further, the main Hanahan and Weinberg “Hallmarks of Cancer” are revisited. In most chapters, the fundamental experiments that led to key concepts, connecting basic biology and biomedicine are highlighted. In the book’s closing section all of these concepts are integrated in clinical studies, where molecular diagnosis as well as the various classical and modern therapeutic strategies are addressed. The book is written in an easy-to-read language, like a one-on-one conversation between the writer and the reader, without compromising the scientific accuracy. Therefore, this book is suited not only for advanced undergraduates and master students but also for patients or curious lay people looking for a further understanding of this shattering disease

Download or read book Integrative Human Biochemistry written by Andrea T. Da Poian and published by Springer Nature. This book was released on 2021-01-04 with total page 685 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers in detail the mechanisms for how energy is managed in the human body. The basic principles that elucidate the reactivity and physical interactions of matter are addressed and quantified with simple approaches. Three-dimensional representations of molecules are presented throughout the book so molecules can be viewed as unique entities in their shape and function. The book is focused on the molecular mechanisms of cellular processes in the context of human physiological situations such as fasting, feeding and physical exercise, in which metabolic regulation is highlighted. Furthermore the book uses key historical experiments that opened up new concepts in biochemistry to further illustrate how the human body functions at molecular level, helping students to appreciate how scientific knowledge emerges. New to this edition: - 30 challenging practical case studies (2-3 at the end of each chapter) based on movies, novels, biographies, documentaries, paintings, and other cultural and artistic creations far beyond canonic academic exercises. - A set of challenging questions and problems in the end of each case study to further engage students with the applications of medical biochemistry - Insights into the answers to the challenging questions to help steer teaching/learning interactions key to productive lectures, PBL (problem-based learning) or traditional tutorials, or e-learning approaches. Advance praise for the second edition: “The Challenging Cases are compelling both from a scientific viewpoint and for the perspective they provide on the history of medicine.” David M. Jameson, University of Hawaii “Using case studies to reinforce the biochemistry lessons is extremely effective – as well as entertaining!” Joseph P. Albanesi, UT Southwestern Medical Center Advance Praise for the first edition: “This textbook provides a modern and integrative perspective of human biochemistry and will be a faithful companion to health science students following curricula in which this discipline is addressed. This textbook will be a most useful tool for the teaching community.” Joan Guinovart Former director of the Institute for Research in Biomedicine, Barcelona, Spain, and former president of the International Union of Biochemistry and Molecular Biology, IUBMB

Download or read book Systems Biology in Drug Discovery and Development written by Qing Yan and published by Humana Press. This book was released on 2010-09-21 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the failing “one-drug-fits-all” model, it has become increasingly necessary to develop personalized medicine that treats whole systems and brings the right drug to the right patient with the right dosages. In Systems Biology in Drug Discovery and Development: Methods and Protocols, leading experts provide a practical, state-of-the-art, and holistic view of the translation of systems biology into better drug discovery and personalized medical practice. While the first part of the book describes cutting-edge technologies and methods in the field, the second part illustrates how the technologies can be applied in science for disease understanding and therapeutic discovery. As a volume in the highly successful Methods in Molecular BiologyTM series, this collection provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and up-to-date, Systems Biology in Drug Discovery and Development: Methods and Protocols covers topics from fundamental concepts to advanced technologies in order to best serve biomedical students and professionals at all levels who are interested in vital integrative studies in molecular biology, genetics, bioinformatics, bioengineering, biochemistry, physiology, pathology, microbiology, immunology, pharmacology, toxicology, drug discovery, and clinical medicine.

Download or read book Antisense Research and Applications written by Stanley T. Crooke and published by CRC Press. This book was released on 1993-05-27 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antisense Research and Applications is a comprehensive review of oligonucleotide research covering molecular biological advances in this field, the current status of antisense drug research, and strategies for future research and therapeutic applications. In bringing together the latest research from an array of authoritative scientists, Antisense Research and Applications provides an integrated conceptual basis for considering oligonucleotide therapeutics. Topics covered in the 32 chapters of this book include nucleic acid structure and function, antisense RNA, medicinal chemistry of oligonucleotides, analogs, pharmacokinetics and toxicology, and activities of current antisense drugs. This volume addresses advances in a broad range of disciplines and is an excellent resource for basic researchers and applied investigators in pharmaceutical laboratories and in such fields as biochemistry and molecular biology.

Download or read book Microarrays for an Integrative Genomics written by Isaac S. Kohane and published by Bradford Books. This book was released on 2003 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introduction to the use of DNA microarrays in functional genomics.