Download or read book Investigation of the N2 Fixing Enzyme Complex from Azotobacter Vinelandii Part I written by Robert Vernon Klucas and published by . This book was released on 1967 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Investigation of the Nitrogenase Enzyme Complex of Azotobacter Vinelandii written by Koven Lamont Hadfield and published by . This book was released on 1970 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Catalysts for Nitrogen Fixation written by Barry E. Smith and published by Springer Science & Business Media. This book was released on 2013-03-20 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological nitrogen fixation provides more than 50% of the total annual input of the essential element nitrogen to world agriculture. Thus, it is of immense agronomic importance and critical to food supplies, particularly in developing countries. This book, with chapters authored by internationally renowned experts, provides a comprehensive and detailed account of the fascinating history of the process - including the surprising discoveries of molybdenum-independent nitrogenases and superoxide-dependent nitrogenase; a review of Man's attempts to emulate the biological process - most successfully with the commercially dominant Haber-Bosch process; and the current state of the understanding art with respect to the enzymes - called nitrogenases - responsible for biological nitrogen fixation. The initial chapters use a historical approach to the biological and industrial processes, followed by an overview of assay methodologies. The next set of chapters focuses on the classical enzyme, the molybdenum nitrogenase, and details its biosynthesis, structure, composition, and mechanism of action as well as detailing both how variants of its two component proteins are constructed by recombinant DNA technology and how computational techniques are being applied. The sophisticated chemical modelling of the metal-containing clusters in the enzyme is reviewed next, followed by a description of the two molybdenum-independent nitrogenases - first, the vanadium-containing enzyme and then the iron-only nitrogenase - together with some thoughts as to why they exist! Then follows an up-to-date treatment of the clearly "non-classical" properties of the superoxide-dependent nitrogenase, which more closely resembles molybdenum-containing hydroxylases and related enzymes, like nitrate reductase, that it does the other nitrogenases. Each chapter contains an extensive list of references. This book is the self-contained first volume of a comprehensive seven-volume series. No other available work provides the up-to-date and in-depth coverage of this series and this volume. This book is intended to serve as an indispensable reference work for all scientists working in this area, including agriculture and the closely related metals-in-biology area; to assist students to enter this challenging area of research; and to provide science administrators easy access to vital relevant information.
Download or read book Biological Nitrogen Fixation written by Frans J. de Bruijn and published by John Wiley & Sons. This book was released on 2015-06-16 with total page 2250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nitrogen is arguably the most important nutrient required by plants. However, the availability of nitrogen is limited in many soils and although the earth's atmosphere consists of 78.1% nitrogen gas (N2) plants are unable to use this form of nitrogen. To compensate , modern agriculture has been highly reliant on industrial nitrogen fertilizers to achieve maximum crop productivity. However, a great deal of fossil fuel is required for the production and delivery of nitrogen fertilizer. Moreover carbon dioxide (CO2) which is released during fossil fuel combustion contributes to the greenhouse effect and run off of nitrate leads to eutrophication of the waterways. Biological nitrogen fixation is an alternative to nitrogen fertilizer. It is carried out by prokaryotes using an enzyme complex called nitrogenase and results in atmospheric N2 being reduced into a form of nitrogen diazotrophic organisms and plants are able to use (ammonia). It is this process and its major players which will be discussed in this book. Biological Nitrogen Fixation is a comprehensive two volume work bringing together both review and original research articles on key topics in nitrogen fixation. Chapters across both volumes emphasize molecular techniques and advanced biochemical analysis approaches applicable to various aspects of biological nitrogen fixation. Volume 1 explores the chemistry and biochemistry of nitrogenases, nif gene regulation, the taxonomy, evolution, and genomics of nitrogen fixing organisms, as well as their physiology and metabolism. Volume 2 covers the symbiotic interaction of nitrogen fixing organisms with their host plants, including nodulation and symbiotic nitrogen fixation, plant and microbial "omics", cyanobacteria, diazotrophs and non-legumes, field studies and inoculum preparation, as well as nitrogen fixation and cereals. Covering the full breadth of current nitrogen fixation research and expanding it towards future advances in the field, Biological Nitrogen Fixation will be a one-stop reference for microbial ecologists and environmental microbiologists as well as plant and agricultural researchers working on crop sustainability.
Download or read book The Nitrogen fixing Enzyme Complex written by Jiann-Chaur Hwang and published by . This book was released on 1968 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetics and Regulation of Nitrogen Fixation in Free Living Bacteria written by Werner Klipp and published by Springer Science & Business Media. This book was released on 2006-01-28 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetics and Regulation of Nitrogen-Fixing Bacteria This book is the second volume of a seven-volume series, which covers all fields of research related to nitrogen fixation - from basic studies through applied aspects to environmental impacts. Volume II provides a comprehensive and detailed source of information concerning the genetics and regulation of biological nitrogen fixation in free-living prokaryotes. This preface attempts to provide the reader with some insight into how this volume originated, how it was planned, and then how it developed over the several years of its production. Once the editorial team was established, the first job was to decide which of the many free-living diazotrophs that have been subjected to genetic analysis should be included in this volume. Would we need to develop specific criteria for selection or would the organisms, in effect, select themselves? Of course, Klebsiella pneumoniae and Azotobacter vinelandii, which have served (and still serve) as the main model organisms for the genetic analysis of diazotrophy, plus some of the other bacteria described in this volume, did indeed select themselves. However, there was considerable discussion surrounding well-characterized fixing species, like Azorhizobium caulinodans and Herbaspirillum seropedicae, both of which are able to fix atmospheric N under free-living conditions.
Download or read book Azospirillum VI and Related Microorganisms written by Istvan Fendrik and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: Azospirillum is a plant growth promoting rhizobacterium used for inoculation of cereal and forage crops. The book covers its physiology, ecology, biochemistry, and molecular biology. The most advanced molecular techniques to understand the regulatory mechanisms of nitrogen fixation and ammonia assimilation, as well as the basis of phytohormone production, are included. In particular, the identification of novel types of promoters, specific regulatory circuits, and new regulatory proteins is described. New insights in the plant growth promoting role of the bacteria through the analysis of their interactions with the plant are presented. Also discussed are field applications, allowing the evaluation of the physiological and agronomic involvement of Azospirillum inoculations.
Download or read book Studies on N2 Fixation by Cell free Extracts of Azotobacter Vinelandii written by L. Merzer and published by . This book was released on 1975 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nitrogen Fixation written by Achim Muller and published by Springer. This book was released on 1983-03 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nitrogen fixation: its scope and importance; Aspects of biochemistry and genetics; Reactions and physicochemical properties of the nitrogenase MoFe proteins; Mossbauer investigation of nitrogenase; Iron-molybdenum cofactor and its complementary protein from mutant organisms; Genetics of nitrogen fixation in free-living organisms; Biochemical genetics of nitrogen fixation in Rhizobium; Regulation and control of nitrogenase activity; Chemical aspects; Thiomolybdates and thiotungstates: their properties and role as ligands in coordination chemistry; The chemistry of the Fe-M-S complexes (M=Mo, W); Iron-molybdenum-sulfur clusters; Dinitrogen complexes and their reactions; Structures of complexes of reduced nitrogen ligands; Overview of 95 Mo NMR; Multisulfur metal sites in enzymes, complexes, clusters and solids: possible relevance for nitrogenase.
Download or read book Nitrogen Fixation in Bacteria and Higher Plants written by R.C. Burns and published by Springer. This book was released on 1975-06-04 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our knowledge of the biochemistry and biophysics of dinitrogen fixa tion has developed rapidly in the 15 years since the first N2-fixing enzyme system was successfully extracted from a bacterium. This peri od has produced a literature that now describes the N2 fixation reac tion and the nitrogenase enzyme itself in sophisticated terms, though a detailed reaction mechanism at the chemical level has not yet emerged. It is the purpose of the present monograph to present an in-depth re view, analysis, and integration of this research as is possible with a non-contributed publication and to relate this work to considera tions of N2 fixation that reach beyond the confines of the biochem ist's laboratory. The first section is directed as much toward the general science read er as toward the specialist. It covers the agricultural origins of man's interest in N2 fixation and also pertinent areas of taxonomy, physiology, and evolution. Ecological aspects of the subject include a comprehensive evaluation of the nitrogen cycle leading to a sub stantially greater estimate of the rate of global N2 fixation than previous ones. The treatment is of a survey fashion, in part to pro vide a general over-view of N2 fixation and in part to provide context for the biochemistry and biophysics that follow in the second section.
Download or read book Structural Analysis of the Reductase Component AnfH of Iron only Nitrogenase from Azotobacter Vinelandii written by Christian Trncik and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Biological nitrogen fixation, the conversion of atmospheric dinitrogen into bioavailable ammonium, is exclusively catalyzed by the enzyme nitrogenase that is present in nitrogen-fixing organisms, the diazotrophs. So far, three different nitrogenase variants, encoded in their corresponding, distinct gene clusters, have been found in nature. Each one of these consists of a catalytic dinitrogenase component and a unique, ATP-dependent reductase, the Fe protein. The three variant nitrogenases differ in the composition of the active site and contain either molybdenum, vanadium or only iron in the dinitrogenase component. Here we present the 2.0 Å resolution crystal structure of the ADP-bound reductase component AnfH of the iron-only nitrogenase from the model diazotroph Azotobacter vinelandii. A comparison of this structure with the ones reported for the two other Fe protein homologs NifH and VnfH in the ADP-bound state shows that all are adopting the same conformation. However, cross-reactivity assays with the three nitrogenase homologs revealed AnfH to be compatible with iron-only nitrogenase and to a lesser degree with the vanadium-containing enzyme, but not with molybdenum nitrogenase
Download or read book Biology of the Nitrogen Cycle written by Hermann Bothe and published by Elsevier. This book was released on 2007 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanism of Substrate Reduction by Nitrogenase written by Nimesh Khadka and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nitrogen (N) is a chemical constituent for almost all biological molecules including proteins, DNA, RNA, lipids and is therefore vital for life. The ultimate source of nitrogen is the atmospheric dinitrogen (N2) but that only becomes bioavailable through a process of nitrogen fixation, the process that converts N2 to ammonia (NH3). The industrial Haber-Bosch process and biological nitrogen fixation account for the majority of nitrogen fixed every year. However, due to its high temperature, pressure and fossil fuel requirements, Haber-Bosch is an expensive process. Every year, approximately 3% of the global energy demand is used to manufacture ammonia through Haber-Bosch process. On the other hand, biological systems produce ammonia by reducing dinitrogen at ambient temperature and pressure using an anaerobic enzyme called nitrogenase. Research in understanding the mechanism of nitrogenase could eventually allow researchers to mimic the enzyme and fix nitrogen efficiently at standard temperature and pressure. In this research nitrogenase of Azotobacter vinelandii was studied to understand the mechanism of delivery of electrons/protons to the active site and how these accumulated reducing equivalents are used for substrates reduction. Through a series of studies, it has been demonstrated that the electrons and protons are added to the active site in a concerted manner which are then stored as bridging hydrides. The accumulated hydrides are used in four different mechanisms, namely reductive elimination, hydride protonolysis, migratory insertion and proton coupled electron transfer, to catalyze the reduction of varieties of unsaturated molecules. This fundamental understanding of molecular detail of nitrogenase catalysis could eventually help in development of more efficient, robust and selective catalysts.
Download or read book Enzymatic Bioelectrocatalysis written by Kenji Kano and published by Springer Nature. This book was released on 2020-11-13 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamental aspects of the electrochemistry and redox enzymes that underlie enzymatic bioelectrocatalysis, in which a redox enzyme reaction is coupled with an electrode reaction. Described here are the basic concept and theoretical aspects of bioelectrocatalysis and the various experimental techniques and materials used to study and characterize related problems. Also included are the various applications of bioelectrocatalysis to bioelectrochemical devices including biosensors, biofuel cells, and bioreactors. This book is a unique source of information in the area of enzymatic bioelectrocatalysis, approaching the subject from a cross-disciplinary point of view.
Download or read book Nitrogen in the Marine Environment written by Edward J. Carpenter and published by Elsevier. This book was released on 2013-10-22 with total page 919 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nitrogen in the Marine Environment provides information pertinent to the many aspects of the nitrogen cycle. This book presents the advances in ocean productivity research, with emphasis on the role of microbes in nitrogen transformations with excursions to higher trophic levels. Organized into 24 chapters, this book begins with an overview of the abundance and distribution of the various forms of nitrogen in a number of estuaries. This text then provides a comparison of the nitrogen cycling of various ecosystems within the marine environment. Other chapters consider chemical distributions and methodology as an aid to those entering the field. This book discusses as well the enzymology of the initial steps of inorganic nitrogen assimilation. The final chapter deals with the philosophy and application of modeling as an investigative method in basic research on nitrogen dynamics in coastal and open-ocean marine environments. This book is a valuable resource for plant biochemists, microbiologists, aquatic ecologists, and bacteriologists.
Download or read book Biological Nitrogen Fixation written by John Raymond Postgate and published by . This book was released on 1972 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molybdenum Enzymes written by Thomas G. Spiro and published by Wiley-Interscience. This book was released on 1985-11-14 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 7 in the Metal Ions in Biology Series, divided into two parts, covers the nitrogenase enzyme complex and the molybdenum redox enzymes. Part one covers the chemistry of Mo-Fe-S clusters and their relationship to nitrogenase, cofactor chemistry and biochemistry of nitrogenase, spectroscopic and electrochemical studies of the Fe-Mo cofactor and Fe-S clusters, and more. Part Two surveys oxo-molybdenum chemistry, discusses the nature of the molybdo-pterin complex, and describes the characteristics of several of the Mo redox enzymes.
