Download or read book Investigating Management and Genetics of Soybean Sudden Death Syndrome Pathogens Fusarium Virguliforme and F Brasiliense written by Mitchell G. Roth and published by . This book was released on 2019 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annual soybean production in the U.S. is worth nearly $40 billion, valued for its oils and protein content. Many pathogens and pests cause significant soybean yield losses each year, but one of the top threats is sudden death syndrome (SDS). At least five fungal species cause soybean SDS globally, but only two have been found in the U.S.; Fusarium virguliforme and F. brasiliense. These soil-borne pathogens infect root tissues and cause root rot, with continued infection leading to foliar interveinal chlorosis, interveinal necrosis, leaf drop, and yield loss. The pathogens are strong saprophytes that can overwinter in soybean and corn residue, so successful management is difficult. Long-term crop rotations and seed treatments with fungicides show some efficacy, but these strategies can be costly for growers. Growers desire genetic resistance to SDS, but no soybean germplasm has shown 100% resistance to SDS to date. Therefore, the overall goals of projects presented in this dissertation were to help improve SDS management and explore the biology and genetics of F. virguliforme and F. brasiliense. To achieve these goals, I developed a risk prediction tool for integration with current SDS management strategies (Chapter 2). This study revealed that pathogen data collected from soil at-planting can be used to accurately model spatial distributions pathogens and model future SDS development and yield loss at a field level. This risk prediction study used a qPCR assay specific for F. virguliforme, but a similar qPCR assay for F. brasiliense did not exist. Therefore, I developed a qPCR assay that can distinguish F. brasiliense from close relatives (Chapter 3). This tool that can be used to generate SDS-prediction models for F. brasiliense and I predict will be valuable in diagnostic labs across the country to distinguish between these two species. To advance our understanding of the biology and genetics of these pathogens, I developed a new protoplast generation and transformation method to generate fluorescent strains of each pathogen (Chapter 4). This chapter is the first to report genetic transformation in F. brasiliense. Furthermore, I used the fluorescent strains to investigate the synergistic role of soil-borne nematodes in SDS (Chapter 5). The interactions between these fungal pathogens and nematodes in vitro show that F. virguliforme and F. brasiliense can colonize immobile nematodes, but suggest that they are not actively vectored into soybean roots by nematodes. The genetic mechanisms of SDS development are poorly understood, so I developed high quality genome sequences for F. virguliforme and F. brasiliense (Chapter 6) and investigated two recognized effector proteins; FvTox1 and FvNIS1 (Chapter 7). The genome assemblies developed here have significantly improved continuity, with improved genome assembly metrics like contig length (N50) and contig number. However, whole-genome alignments between F. virguliforme and F. brasiliense from soybean (Glycine max) or dry bean (Phaseolus vulgaris) did not reveal obvious mobile pathogenicity chromosomes that have been observed in the close relative F. oxysporum. However, these genome resources should facilitate discovery of new fungal effector proteins like FvTox1 and FvNIS1. Interestingly, my results show that FvNIS1 is able to induce a hypersensitive response in tobacco, while FvTox1 is not, suggesting a conserved mechanism between soybean and tobacco for FvNIS1 recognition. Overall, this work provides valuable tools for managing and studying SDS-causing fungi, while also revealing insights into the genetics and genomics of the SDS-causing pathogens F. virguliforme and F. brasiliense.
Download or read book Insights of Chemical Cultural and Genetic Exploration for Soybean Sudden Death Syndrome Management and Fusarium Virguliforme written by Amy Marie Baetsen-Young and published by . This book was released on 2019 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soybean sudden death syndrome, caused by Fusarium virguliforme, is a key limitation in reaching soybean (Glycine max) yield potential, stemming from limited disease management through cultural practices and partial host resistance. The research within this thesis reveals the economic potential of fungicide seed treatment SDS fluopyram to alleviate yield loss, provides insights into field management of F. virguliforme and highlights transcriptomic plasticity of diverse host-fungal interactions. Previously, farm level studies have found the fungicide seed treatment of fluopyram profitable, yet the benefit across an aggregate level of soybean production at risk to SDS yield loss is unknown. To estimate economic benefits of fluopyram adoption in SDS at risk acres, in the light of U.S public research and outreach costs, an economic surplus approach was applied to calculate ex ante net benefits from 2018 to 2032. Through this framework of fluopyram adoption for alleviation of SDS associated yield losses, we estimated a net benefit of $5,829 million over 15 years, considering public seed treatment research costs from 2014 to present and future extension communication. While chemical seed treatments aid disease management of SDS, the ability of this pathogen to colonize asymptomatic hosts may increase the prevalence of F. virguliforme. Thus, the impact of cultural tactics upon F. virguliforme colonization of an asymptomatic host, and the ability of this colonization to alter subsequent SDS symptoms when rotated to soybean were explored. The exploration of tillage, and residue management across four U.S. states provided clarity to variable reports, revealing that no-tillage inconsistently enhances F. virguliforme colonization of corn and soybean roots, while corn residue did not alter pathogen colonization. Alternatively, an asymptomatic host provides a unique application to discover genetic factors facilitating soybean sudden death syndrome through investigation host-fungal interactions. Exploring this plant disease through a comparative orthologous mRNA-Seq on soybean and corn hosts under colonization of F. virguliforme uncovered transcriptional responses enabling a robust defense response in corn, and delayed immune induction within soybean permitting pathogenic colonization and susceptibility. To colonize both hosts, F. virguliforme exhibited a massive transcriptional rewiring of an infection program. Transcriptomic responses suggest, F. virguliforme is less suited for colonization of monocots by delayed colonization, and lower induction of CAZymes and effector proteins. Integration of the data generated through the mRNA-Seq experiments, including a micro-like RNA-Seq analysis of soybean host during colonization by F. virguliforme revealed an intimate communication between the plant and fungal pathogen; we posit that a micro-like RNA cross-talk potentially regulates host susceptibility. Overall, several hypotheses were generated surrounding hemibiotrophic enhancement of host senescence, and fungal ecological plasticity through transcriptomic reprogramming, which will deliver transparency upon a currently difficult and enigmatic syndrome.
Download or read book Identification and Characterization of Pathogenicity Genes in Fusarium Virguliforme the Causal Agent of Sudden Death Syndrome SDS in Soybean written by Saara Mansouri and published by . This book was released on 2012 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fusarium virguliforme is a soil-borne pathogen that causes sudden death syndrome (SDS) disease in soybean. SDS is one of the most significant diseases of soybean in the United States. Fungal infection results in root and crown rot as well as SDS typical foliar symptoms including chlorosis, necrosis and complete defoliation. The use of soybean cultivars tolerant to SDS is still the most effective way to overcome the disease. On the other hand, the fungal isolates are known to have varied levels of aggressiveness on soybean indicated by the field and greenhouse experiments. Understanding the pathogen and its defense mechanism is the first step in exploring the pathogen-plant interaction. Therefore, the primary aim of this research was to elucidate the mechanism behind F. virguliforme response to soybean defense mechanisms. We further attempted to identify chromosome length polymorphism among F. virguliforme isolates and characterize the possible relationship to their level of aggressiveness. The findings are instrumental in identifying novel pathogenicity such as the ones involved in phytotoxin production, fungicide resistance and aggressiveness.
Download or read book Molecular Diagnostics Epidemiology and Population Genetics of the Soybean Sudden Death Syndrome Pathogen Fusarium Virguliforme written by Jie Wang and published by . This book was released on 2016 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Mapping of Resistance to Soybean Sudden Death Syndrome and Soybean Oil Quality written by Paul Joseph Collins and published by . This book was released on 2019 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soybean (Glycine max) is the world's leading oilseed crop and is a critical source of protein for poultry and swine production. Soybean production is limited by many biotic factors including soybean sudden death syndrome (SDS) which is caused by a soil-borne fungal pathogen, Fusarium virguliforme. Effective management methods for soybean sudden death syndrome include long-term rotations, fluopyram seed treatment, and planting SDS resistant varieties. Host resistance to F. virguliforme is a quantitative resistance, as it is controlled by many genes, largely of small effect. To more efficiently breed SDS resistant soybean varieties, researchers have sought to identify the loci on the soybean genome responsible for SDS-resistance. Three recombinant inbred line (RIL) populations were evaluated for foliar SDS resistance at a naturally infested field site in Decatur, MI during the 2014 and 2015 growing seasons. These populations segregated for SDS resistance, as they were derived from a parent resistant to SDS and a parent susceptible to SDS. The parents and a subset of RILs from each population were genotyped with the SoySNP6K Illumina Infinium BeadChip. Linkage maps unique to each population were constructed using JoinMap ver. 2. Composite interval mapping was done using WinQTLCartographer (ver. 2.5). Six quantitative trait loci (QTL) were identified to be associated with SDS resistance. Three of the QTL associated with SDS resistance were identified across multiple years and/or populations. While biotic factors, such as SDS, work to limit soybean production, soybean quality factors, such as oil quality, can offer new production opportunities. Soybean oil is predominantly composed of five fatty acids: palmitic acid (11%), stearic acid (4%), oleic acid (25%), linoleic acid (52%), and linolenic acid (8%). While there is little variability in most commodity soybean varieties for fatty acid content, soybean breeders have been able to introduce oil quality traits into the soybean germplasm. Oil quality traits for soybean oil include high oleic acid content (>75%), low linolenic acid content (
Download or read book Studies of Fusarium Virguliforme Causing Sudden Death Syndrome of Soybean written by Ramiz Mahdi Salih Alabdalsaid and published by . This book was released on 2021 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fusarium virguliforme is one of the important pathogens on soybeans, and it is a soil borne pathogen. This pathogen causes sudden death syndrome on soybean (SDS). The fungus remains in the soil or on the plant residues for many years in the form of chlamydospores. When the appropriate conditions are available for the infection, the pathogen penetrates the soybean's roots and causes symptoms on the roots that appear in the form of reddish-brown, discoloration and necrosis. On the leaves the symptoms appear in the form of irregular spots shape. In the development of the infection the fall of leaves, abortion of flowers and pods occur. Due to the economic importance of the disease and the fungus that causes it. This study conducted to gain more information and understand the behavior of the pathogen. The results study of the effect of some culture media, pH and nitrogen sources on F. virguliforme growth in vitro showed that the pathogen has able to grow on all culture media that used. on culture media Czapek Dox agar (CDA) and Malt Extract Agar (MEA), the F. virguliforme was grown fast to cover plate in 8 days of incubation. Potato Sucrose Agar (PSA) and Malt Extract Agar (MEA) were the best culture media for fungal sporulation while the lowest number of spores was on Glucose yeast peptone Agar (GYPA). Also, the study showed the best pH for radial growth was 7 at 20, 25 and 30 C. But the radial growth of Fungus was slow at pH 10. The fungal sporulation at pH 6 was good comparing to fungal sporulation at pH 10 which was less. The radial growth of fungus was affected by different nitrogen sources and concentration. In general, the ammonium sulphate was not suitable for fungal radial growth and The best fungal sporulation was on media with peptone 2 g comparing with other nitrogen sources. In the second chapter, the results of histopathology of roots for two soybean varieties infected by F. virguliforme showed that the pathogen uses the same strategy to attack the roots of soybean varieties, penetration and colonization. The pathogen can grow intercellular and intracellular. During infection the pathogen causes damage to the root cells. Accumulating of materials inside root's cells because pathogen invasion. The last chapter in this study is, effect light on F. virguliforme growth. The study showed that the radial growth of the fungus was the maximum under continuous darkness comparing to fungal radial growth under light/dark cycle 12/12 LD, 16/8 LD and 18/6 LD. In addition to that colorful bands were formed when the fungus grew under under light/dark cycle. The results showed the ability of pathogen to cause Lesion on soybean stems under light/dark cycle. Also, using two inoculum 105, 106 of pathogen to test it pathogenicity on soybean roots showed no significant differences.
Download or read book Soilborne Microbial Plant Pathogens and Disease Management Volume One written by P. Narayanasamy and published by CRC Press. This book was released on 2019-10-08 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soilborne microbial plant pathogens including oomycetes, fungi, bacteria and viruses cause several economically important destructive diseases and the symptoms of infection can be recognized only after the pathogen has invaded many tissues primarily vascular tissues of susceptible plants. This condition places formidable challenges in investigating different aspects of host-microbial pathogen interactions. Early detection of infection and precise identification, differentiation, and quantification of the microbial plant pathogens in plants, soil and water sources are essential requirements for development of effective tactics to reduce the incidence and spread of the diseases caused by them. As the microbial plant pathogens differ in their virulence and sensitivity to the environment and chemicals applied, it is imperative to assess the extent of variability in the concerned pathogens. This first volume of a two-volume set introduces disease-causing microorganisms including oomycetes, fungi, bacteria, and viruses found in soils. It focuses on the biology, detection, and identification of soilborne bacterial, fungal, and viral plant pathogens. This volume discusses various techniques based on biological, immunological and genetic properties of the pathogens indicating their advantages and limitations for selecting the appropriate technique to fulfill the requirements. Features: Presents techniques useful for detection, identification, quantification of microbial plant pathogens in plants, soil, and irrigation water from waterbodies. Highlights subversive activities of viruses, resulting in the breakdown of host defense systems. Discusses RNA silencing in infected plants by viruses and posttranscriptional gene silencing (PTGS) functioning as an endogenous mechanism in plants against virus infection. Presents information on methods of assessment of genetic variability and sensitivity of microbial plant pathogens to chemicals and adverse environmental conditions.
Download or read book Forest Pathology and Plant Health written by Matteo Garbelotto and published by MDPI. This book was released on 2018-04-13 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Forest Pathology and Plant Health" that was published in Forests
Download or read book Plant Diseases Caused by Dickeya and Pectobacterium Species written by Frédérique Van Gijsegem and published by Springer Nature. This book was released on 2021-01-04 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a detailed review of many different aspects of pathogens, from the effects of single base pair mutations to large-scale control options, bringing into a single volume over 100 years of findings from thousands of researchers worldwide. Diseases caused by soft rot Pectobacteriaceae (SRP) are a major cause of loss to crop, vegetables and ornamental plants worldwide, and have been found on all continents except Antarctica. While different aspects of the SRP have appeared in other books on plant disease, no book, until now, has been dedicated solely to them.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 868 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fungi and Food Spoilage written by John I. Pitt and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 599 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed as a laboratory guide for the food microbiologist, to assist in the isolation and identification of common food-borne fungi. We emphasise the fungi which cause food spoilage, but also devote space to the fungi commonly encountered in foods at harvest, and in the food factory. As far as possible, we have kept the text simple, although the need for clarity in the descriptions has necessitated the use of some specialised mycological terms. The identification keys have been designed for use by microbiologists with little or no prior knowledge of mycology. For identification to genus level, they are based primarily on the cultural and physiological characteristics of fungi grown under a standardised set of conditions. The microscopic features of the various fungi become more important when identifying isolates at the species level. Nearly all of the species treated have been illustrated with colony photographs, together with photomicrographs or line drawings. The photomicrographs were taken using a Zeiss WL microscope fitted with Nomarski interference contrast optics. We are indebted to Mr W. Rushton and Ms L. Burton, who printed the many hundreds of photographs used to make up the figures in this book. We also wish to express out appreciation to Dr D.L. Hawksworth, Dr A.H.S.
Download or read book Diversity and Benefits of Microorganisms from the Tropics written by João Lucio de Azevedo and published by Springer. This book was released on 2017-06-10 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the diversity of tropical microorganisms and its applications in agriculture, renewable energy production and environmental protection. It covers several tropical habitats such as rain forests, mangroves, sea and river waters and describes how microorganisms isolated from these regions can be used to control insects and plant diseases, to improve sugar cane and biofuels production among other applications. The book also aims to bring researchers’ attention to the potential of tropical microorganisms for biotechnological purposes, an area that is still far from being well explored.
Download or read book Fungicides for Field Crops written by Daren Mueller and published by . This book was released on 2013 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungicides for Field Crops provides an overview of the current knowledge of fungicides and their use on field crops. This comprehensive book, which includes the contributions of 40 professionals from 20 universities and other organizations, combines past knowledge about fungicides with recent developments in the realm of field crop fungicides. Fungicides for Field Crops highlights the use of fungicides as key tools in the management of important diseases of field crops. Management is presented as a decision-making process--one in which factors as diverse as weather conditions and economics must be considered. Having a more complete understanding of fungicides will inform that decision making and help determine when fungicides should be included as part of a management plan. Parts I, II, and III establish a foundation of knowledge about fungicide use, addressing basic terms and concepts, key factors in decision making, and concerns for fungicide stewardship, respectively. Part IV presents details about using fungicides to manage diseases of 16 field crops. Each crop is treated in a separate section that begins with a discussion of general issues of foliar application and seed treatment and ends with an inclusive table identifying diseases of that crop, including what causes them and how fungicides may be used to treat them. 131 color photographs illustrate disease symptoms and show techniques and enhance the key concepts described in the text.
Download or read book Soybean written by Hany El-Shemy and published by BoD – Books on Demand. This book was released on 2011-11-02 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Worldwide, soybean seed proteins represent a major source of amino acids for human and animal nutrition. Soybean seeds are an important and economical source of protein in the diet of many developed and developing countries. Soy is a complete protein and soyfoods are rich in vitamins and minerals.Soybean protein provides all the essential amino acids in the amounts needed for human health. Recent research suggests that soy may also lower risk of prostate, colon and breast cancers as well as osteoporosis and other bone health problems and alleviate hot flashes associated with menopause. This volume is expected to be useful for student, researchers and public who are interested in soybean.
Download or read book Industrially Important Fungi for Sustainable Development written by Ahmed M. Abdel-Azeem and published by Springer Nature. This book was released on 2021-06-18 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungi are an understudied, biotechnologically valuable group of organisms. Due to their immense range of habitats, and the consequent need to compete against a diverse array of other fungi, bacteria, and animals, fungi have developed numerous survival mechanisms. However, besides their major basic positive role in the cycling of minerals, organic matter and mobilizing insoluble nutrients, fungi have other beneficial impacts: they are considered good sources of food and active agents for a number of industrial processes involving fermentation mechanisms as in the bread, wine and beer industry. A number of fungi also produce biologically important metabolites such as enzymes, vitamins, antibiotics and several products of important pharmaceutical use; still others are involved in the production of single cell proteins. The economic value of these marked positive activities has been estimated as approximating to trillions of US dollars. The unique attributes of fungi thus herald great promise for their application in biotechnology and industry. Since ancient Egyptians mentioned in their medical prescriptions how they can use green molds in curing wounds as the obvious historical uses of penicillin, fungi can be grown with relative ease, making production at scale viable. The search for fungal biodiversity, and the construction of a living fungi collection, both have incredible economic potential in locating organisms with novel industrial uses that will lead to novel products. Fungi have provided the world with penicillin, lovastatin, and other globally significant medicines, and they remain an untapped resource with enormous industrial potential. Volume 1 of Industrially Important Fungi for Sustainable Development provides an overview to understanding fungal diversity from diverse habitats and their industrial application for future sustainability. It encompasses current advanced knowledge of fungal communities and their potential biotechnological applications in industry and allied sectors. The book will be useful to scientists, researchers, and students of microbiology, biotechnology, agriculture, molecular biology, and environmental biology.
Download or read book Toxigenic Fusarium Species Identity and Mycotoxicology written by W. F. O. Marasas and published by Penn State University Press. This book was released on 1984 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: The taxonomy of fungi in the genus Fusarium is complex and confusing because of the different taxonomic systems in use in various parts of the world. Taxonomy of the genus is further complicated by its extreme variability in culture and by the fact that Fusarium species mutate and degenerate rapidly in culture. This situation has led to great confusion in the extensive literature on Fusarium mycotoxicoses because the same fungus is known under a variety of different names in different countries. This book, a comparative taxonomic study of Fusarium species reported to be toxigenic in published findings, represents the first serious attempt to clarify the confusion in the taxonomy and nomenclature of toxigenic Fusarium species. It is a catalog of the isolates in the International Toxigenic Fusarium Reference Collection, which is made up of isolates collected from research workers around the world. Information on each isolate includes the correct identification, references to publications in which the isolate is cited, and information on the toxic properties of and mycotoxins reported to be produced by each isolate. The nomenclature used follows the system given in Fusarium Species: An Illustrated Manual for Identification. These two volumes will be useful to anyone interested in the mycotoxins produced by Fusarium species.
Download or read book Biocontrol Potential and its Exploitation in Sustainable Agriculture written by Rajeev K. Upadhyay and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant based biotechnology has come to represent a means of mitigating the problems of global food security in the twenty-first century. Products and processes in agriculture are increasingly becoming linked to science and cutting edge technology, to enable the engineering of what are in effect, designer plants. One of the most successful , non-chemical approaches to pest management and disease control is biological control, which seeks a solution in terms of using living organisms to regulate the incidence of pests and pathogens, providing a natural control' while still maintaining the biological balance with the ecosystem. This volume, (the first of two), addresses the different types of biocontrol for different pests, namely, crop diseases, weeds and nematodes, and details the biology of both the pest and its enemies, which is vital for efficient use of biological control. The book has numerous contributors who are authorities in their fields, and would be an asset to those who have interest in sustainable agriculture and crop productivity.
