Download or read book Investigating Cellular Nanoscale with X rays written by Clément Hémonnot and published by Göttingen University Press. This book was released on 2016 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advances and technical improvements of X-ray imaging techniques, taking advantage of X-ray focussing optics and high intensity synchrotron sources, nowadays allow for the use of X-rays to probe the cellular nanoscale. Importantly, X-rays permit thick samples to be imaged without sectioning or slicing. In this work, two macromolecules, namely keratin intermediate filament (IF) proteins and DNA, both essential components of cells, were studied by X-ray techniques. Keratin IF proteins make up an integral part of the cytoskeleton of epithelial cells and form a dense intracellular network of bundles. This network is built from monomers in a hierarchical fashion. Thus, the keratin structure formation spans a large range of length scales from a few nanometres (monomers) to micrometres (networks). Here, keratin was studied at three different scales: i) filaments, ii) bundles and iii) networks. Solution small-angle X-ray scattering revealed distinct structural and organisational characteristics of these highly charged polyelectrolyte filaments, such as increasing radius with increasing salt concentration and spatial accumulation of ions depending on the salt concentration. The results are quantified by employing advanced modelling of keratin IFs by a core cylinder fl anked with Gaussian chains. Scanning micro- diffraction was used to study keratin at the bundle scale. Very different morphologies of keratin bundles were observed at different salt conditions. At the network scale, new imaging approaches and analyses were applied to the study of whole cells. Ptychography and scanning X-ray nano-diffraction imaging were performed on the same cells, allowing for high resolution in real and reciprocal space, thereby revealing the internal structure of these networks. By using a fitting routine based on simulations of IFs packed on a hexagonal lattice, the radius of each fi lament and distance between fi laments were retrieved. In mammalian cells, each nucleus contains 2 nm-thick DNA double helices with a total length of about 2 m. The DNA strands are packed in a highly hierarchical manner into individual chromosomes. DNA was studied in intact cells by visible light microscopy and scanning X-ray nano-diffraction, unveiling the compaction und decompaction of DNA during the cell cycle. Thus, we obtained information on the aggregation state of the nuclear DNA at a real space resolution on the order of few hundreds nm. To exploit to the reciprocal space information, individual diffraction patterns were analysed according to a generalised Porod’s law at a resolution down to 10 nm. We were able to distinguish nucleoli, heterochromatin and euchromatin in the nuclei and follow the compaction and decompaction during the cell division cycle.

Download or read book Investigating Cellular Nanoscale with X rays from Proteins to Networks written by Clément Y. J. Hémonnot and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In this work, two macromolecules, both essential components of cells (keratin protein and DNA nucleic acid) were studied by X-ray techniques. I described the implementation and use of different methods to investigate these biopolymers at the nanoscale: solution SAXS, ptychography and scanning X-ray diffraction. Keratin proteins belong to the cytoskeleton of epithelial cells forming a dense network. This network is built from monomers in a stepwise fashion. First, two monomers assemble to form a parallel heterodimer (about 2~nm diameter). Second, tetramers (4 to 5~nm diameter) are formed by ...

Download or read book Investigating Cellular Nanoscale with X rays from Proteins to Networks written by Clément Y. J. Hémonnot and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this work, two macromolecules, both essential components of cells (keratin protein and DNA nucleic acid) were studied by X-ray techniques. I described the implementation and use of different methods to investigate these biopolymers at the nanoscale: solution SAXS, ptychography and scanning X-ray diffraction. Keratin proteins belong to the cytoskeleton of epithelial cells forming a dense network. This network is built from monomers in a stepwise fashion. First, two monomers assemble to form a parallel heterodimer (about 2~nm diameter). Second, tetramers (4 to 5~nm diameter) are formed by ...

Download or read book Nanoscale Photonic Imaging written by Tim Salditt and published by Springer Nature. This book was released on 2020-06-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.

Download or read book Scanning X ray nano diffraction on eukaryotic cells written by Britta Weinhausen and published by Göttingen University Press. This book was released on 2014 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-rays provide an ideal probe for studying structures at the nano-scale and are routinely employed for investigating the structure and the composition of biological systems, making use of the variety of different techniques. By raster scanning the sample with a small beam, structural information obtained from individual scattering patterns in reciprocal space can be combined with positional information in real space. In this work, scanning X-ray diffraction using a nano-focused beam was applied to samples of biological cells in order to probe the structure of cytoskeletal bundles and networks of keratin intermediate filaments. Cellular samples were prepared using different methods, starting from well-established freeze-dried samples and going on to fixed-hydrated and finally living cells. In this context, the development of X-ray compatible microfluidic devices allowing for measurements on living cellular samples was an important aspect. Comparing the scattering signal from freeze-dried, fixed-hydrated and living cells, differences between the sample types at length scales of several tens of nanometers were determined. The successful application to hydrated and living cells further demonstrates the potential for structural analysis at hardly accessible length scales in native samples. Published: 2014

Download or read book Investigations on Joule heating applications by multiphysical continuum simulations in nanoscale systems written by Feuchter, Manuel Klaus Ludwig and published by KIT Scientific Publishing. This book was released on 2014-10-01 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work furthers the overall understanding of a 3w-measurement, by considering previously unexamined macroscopic influence factors within measurement by Finite Element simulations (FES). Moreover, new measuring configurations are developed to determine (an)isotropic thermal conductivities of nanoscale samples. Since no analytic solutions are available for these configurations, a new evaluation methodology is presented to determine emergent thermal conductivities by FES and Neural Networks.

Download or read book X Rays in Nanoscience written by Jinghua Guo and published by John Wiley & Sons. This book was released on 2011-09-22 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: An up-to-date overview of the different x-ray based methods in the hot fields of nanoscience and nanotechnology, including methods for imaging nanomaterials, as well as for probing the electronic structure of nanostructured materials in order to investigate their different properties. Written by authors at one of the world's top facilities working with these methods, this monograph presents and discusses techniques and applications in the fields of x-ray scattering, spectroscopy and microscope imaging. The resulting systematic collection of these advanced tools will benefit graduate students, postdocs as well as professional researchers.

Download or read book Investigation of Cells and Cellular Components with X Rays in Liquid Sample Environments written by Jan-Philipp Burchert and published by . This book was released on 2024 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to their short wavelength, X-rays offer a high spatial resolution and a high ability to penetrate soft condensed matter. Therefore, X-rays are well-suited for investigating structures and structural changes in in-vitro systems as well as in intact biological cells and tissues. For such investigations, suitable sample environments need to be developed and used to avoid effects from surfaces or sample preparation. The applied sample environments need be compatible with both the sample under investigation and the X-ray radiation. In this thesis, we use small-angle X-ray scattering (SAXS) a...

Download or read book Unraveling the Safety Profile of Nanoscale Particles and Materials written by Andreia Ferreira de Castro Gomes and published by BoD – Books on Demand. This book was released on 2018-03-21 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: As nanomaterials become increasingly present in our daily lives, pertinent questions regarding their safety arise. Nanomaterial risk assessment, as in other areas, directs much of the effort worldwide in defining guidelines that may be translated into national or international directives. Nanomaterials encompass different entities, from nanoparticles to nanostructured materials, with specific effects over cells, tissues, organisms and ecosystems depending on their biophysical characteristics. Such interactions will directly affect the impact of novel nanotechnologies. This book aims to provide the reader with a comprehensive overview of the current state of the art in nanotoxicology, featuring the most important developments and critical issues regarding the use of and exposure to nanoparticles.

Download or read book Synchrotron Radiation in Materials Science written by Chunhai Fan and published by John Wiley & Sons. This book was released on 2018-05-29 with total page 846 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meeting the long-felt need for in-depth information on one of the most advanced material characterization methods, a top team of editors and authors from highly prestigious facilities and institutions covers a range of synchrotron techniques that have proven useful for materials research. Following an introduction to synchrotron radiation and its sources, the second part goes on to describe the various techniques that benefit from this especially bright light, including X-ray absorption, diffraction, scattering, imaging, and lithography. The thrid and final part provides an overview of the applications of synchrotron radiation in materials science. bridging the gap between specialists in synchrotron research and material scientists, this is a unique and indispensable resource for academic and industrial researchers alike.

Download or read book Metal based Anticancer Agents written by Angela Casini and published by Royal Society of Chemistry. This book was released on 2019-04-05 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metal-based anticancer drugs are among the most successful therapeutic agents, as evidenced by the frequent prescription of selected platinum and arsenic compounds to patients. Metal-based Anticancer Agents covers the interdisciplinary world of inorganic drug discovery and development by introducing the most prominent compound classes based on different transition metals, discussing emerging concepts and enabling methods, as well as presenting key pre-clinical and clinical aspects. Recent progress on the unique features of next-generation targeted metal-based anticancer agents, including supramolecular coordination complexes used for both therapy and drug delivery, promise a bright future beyond the benefits of pure cytotoxic activity. With contributions from global leaders in the field, this book will serve as a useful reference to established researchers as well as a practical guide to those new to metallodrugs, and postgraduate students of medicinal chemistry and metallobiology.

Download or read book Small Angle Scattering and Diffraction written by Margareth Kazuyo Kobayashi Dias Franco and published by BoD – Books on Demand. This book was released on 2018-06-06 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reasoned and based on the difference between discovery and invention, according to the traditional conception, science can be distinguished between basic science and applied science. Nevertheless, we know that the sciences are inseparable. A century or more ago, Louis Pasteur said "there is no applied science, there are applications of science." With this assertion, he establishes the logic of complementarity between them. Science certainly goes beyond its own material application and brings us to issues that have intrigued humanity for a long time. During the many years that we have been working with techniques of material characterization, we observed that this complementarity was not always understood by the researchers. In line with the reasoning that the technique joined with science generates technology, the application of techniques that use x-ray and neutron sources seems to us of fundamental importance for the development of technology. In this way, we present in this book how the existing technology of material characterization can contribute to science and applied technology. The authors who contributed with this book sought to show the importance of applying the existing techniques in the development of their works.

Download or read book Theoretical Concepts of X Ray Nanoscale Analysis written by Andrei Benediktovich and published by Springer Science & Business Media. This book was released on 2013-09-07 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a concise survey of modern theoretical concepts of X-ray materials analysis. The principle features of the book are: basics of X-ray scattering, interaction between X-rays and matter and new theoretical concepts of X-ray scattering. The various X-ray techniques are considered in detail: high-resolution X-ray diffraction, X-ray reflectivity, grazing-incidence small-angle X-ray scattering and X-ray residual stress analysis. All the theoretical methods presented use the unified physical approach. This makes the book especially useful for readers learning and performing data analysis with different techniques. The theory is applicable to studies of bulk materials of all kinds, including single crystals and polycrystals as well as to surface studies under grazing incidence. The book appeals to researchers and graduate students alike.

Download or read book Materials Discovery and Design written by Turab Lookman and published by Springer. This book was released on 2018-09-22 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the current status, challenges and future directions of data-driven materials discovery and design. It presents the analysis and learning from data as a key theme in many science and cyber related applications. The challenging open questions as well as future directions in the application of data science to materials problems are sketched. Computational and experimental facilities today generate vast amounts of data at an unprecedented rate. The book gives guidance to discover new knowledge that enables materials innovation to address grand challenges in energy, environment and security, the clearer link needed between the data from these facilities and the theory and underlying science. The role of inference and optimization methods in distilling the data and constraining predictions using insights and results from theory is key to achieving the desired goals of real time analysis and feedback. Thus, the importance of this book lies in emphasizing that the full value of knowledge driven discovery using data can only be realized by integrating statistical and information sciences with materials science, which is increasingly dependent on high throughput and large scale computational and experimental data gathering efforts. This is especially the case as we enter a new era of big data in materials science with the planning of future experimental facilities such as the Linac Coherent Light Source at Stanford (LCLS-II), the European X-ray Free Electron Laser (EXFEL) and MaRIE (Matter Radiation in Extremes), the signature concept facility from Los Alamos National Laboratory. These facilities are expected to generate hundreds of terabytes to several petabytes of in situ spatially and temporally resolved data per sample. The questions that then arise include how we can learn from the data to accelerate the processing and analysis of reconstructed microstructure, rapidly map spatially resolved properties from high throughput data, devise diagnostics for pattern detection, and guide experiments towards desired targeted properties. The authors are an interdisciplinary group of leading experts who bring the excitement of the nascent and rapidly emerging field of materials informatics to the reader.

Download or read book World Scientific Reference On Plasmonic Nanomaterials Principles Design And Bio applications In 5 Volumes written by and published by World Scientific. This book was released on 2022-03-04 with total page 2475 pages. Available in PDF, EPUB and Kindle. Book excerpt: World Scientific Reference on Plasmonic Nanomaterials: Principles, Design and Bio-applications is a book collection that encompasses multiple aspects of the exciting and timely field of nanoplasmonics, under the coordination of international plasmonic nanomaterials expert, Dr Luis Liz-Marzán. Plasmonics has a long history, from stained glass in ancient cathedrals, through pioneering investigations by Michael Faraday, all the way into the nanotechnology era, where it blossomed into an extremely active field of research with potential applications in a wide variety of technologies.Given the breadth of the materials, phenomena and applications related to plasmonics, this Reference Set offers a collection of chapters within dedicated volumes, focusing on the description of selected phenomena, with an emphasis in chemistry as an enabling tool for the fabrication of, often sophisticated, plasmonic nanoarchitectures and biomedicine as the target application.Basic principles of surface plasmon resonances are described, as well as those mechanisms related to related phenomena such as surface-enhanced spectroscopies or plasmonic chirality. Under the guidance of theoretical models, wet chemistry methods have been implemented toward the synthesis of a wide variety of nanoparticles with different compositions and tailored morphology. But often the optimal nanoarchitecture requires post-synthesis treatments, including functionalization of nanoparticle surfaces, application of external stimuli toward self-assembly into well-defined supraparticle structures and so-called supercrystals. All such nanomaterials can find applications in various biomedical aspects, most often in relation to diagnosis, through either the detection of disease biomarkers at extremely low concentrations or the design of bioimaging methods for in vivo monitoring. Additionally, novel therapeutic tools can also profit from plasmonic nanomaterials, such as photothermal therapy or nanocatalysis.The reference set thus offers comprehensive information of an extremely active subset within the world of plasmonic nanomaterials and their applications, which aims at not just collecting existing knowledge but also promoting further research and technology transfer into the market and the clinic.

Download or read book Nanoscale Studies of Strongly Correlated Electron Materials with Coherent X Rays written by Nelson Hua and published by . This book was released on 2020 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: Emergent behaviors arising from strongly correlated electron materials continue to puzzle physicists as there are still no clear explanations behind the underpinning mechanisms. The key hides in the collective behavior originating from the interactions among the electronic degrees of freedom, and the objective is to use various experimental probes to investigate the spin, charge, orbital and lattice contributions and their correlated behavior. This dissertation presents a study into how several advanced coherent x-ray techniques can be used to investigate the nanoscale landscape, both spatially and temporally, of strongly correlated electron materials to understand both the underlying physics and how to incorporate these materials into technological devices. In particular, resonant elastic x-ray scattering and x-ray photon correlation spectroscopy are used to study the charge and orbital order tied to the metal-insulator transition in magnetite (Fe3O4), first observed by Verwey back in 1939. The results presented in Chapter 3 suggest that trimeron fluctuations due to thermal electron hopping among Fe 3d orbital sites play a vital role in the metal-insulator transition. Chapter 4 is an investigation into electron-phonon coupling times in elemental chromium using ultrafast x-ray pump-probe. Chapters 5 and 6 focus on the use of x-ray nanodiffraction to spatially resolve the strain in square LSMO nanoislands and the electrical-induced strain propagation in a ferroelectric/ferromagnetic (PZT/LSMO) heterostructure. Understanding the spatial extent of the correlation between strain to both the electric polarization and magnetic domains in these materials is essential for designs in sensory and computing architectures. Finally, Chapter 7 presents a simulation of a double probe x-ray speckle visibility spectroscopy experiment, a technique that relies on the change of contrast on an area detector to resolve dynamic timescales in the femto- to nanosecond range. Based on the simulated results, an analysis method that relies on selective binning of the detector image pixels is proposed to overcome the obstacles presented by an experimental imperfections such as an angular misalignment between the two probes.

Download or read book Applications Of X ray Techniques To Nanomaterials For Energy Research written by Li-chyong Chen and published by World Scientific. This book was released on 2024-01-22 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomaterials have become a key component for energy-related applications. Their design principle, synthesis and applications are well discussed in various scientific and engineering books, but a gap remains in discussions regarding the application of cutting-edge X-ray techniques to these materials. This volume provides insights from the latest development of X-ray techniques to investigate nanomaterials in specific energy fields, bridging the gap between X-ray analytical scientists and material researchers.We aim to provide researchers with a tool to choose suitable X-ray techniques, carry them out with the right procedure, and analyze the data to give the best reliable results. The approach is microscopic and specific. Among the applications emphasized by the chapters in this book are x-ray techniques in heterogeneous catalysis, electrocatalysis for fuel cells, photocatalysis for water splitting and carbon dioxide reduction, organic photovoltaics, and other energy-related applications.