Download or read book Intravital Microscopy Imaging of Leukocytes written by Elzbieta Kolaczkowska and published by Frontiers Media SA. This book was released on 2020-12-01 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Download or read book Tracking Colliding Cells in Intravital Video Microscopy written by Nhat Huynh Khanh Nguyen and published by . This book was released on 2009 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leukocyte motion represents an important component in the innate immune re¬sponse to infection. Intravital microscopy is a powerful tool as it enables in vivo imaging of leukocyte motion. In inflammatory conditions, leukocytes may exhibit various motion behaviors such as flowing, rolling, and adhering. With many cells moving at a variety of speeds, collisions occur. These collisions result in abrupt changes in the motion and appearance of leukocytes. Analyzing the motion behavior of leukocytes is a difficult task. Manual analysis is tedious, error-prone, and could introduce technician-related bias. Automatic tracking methods are also challenging due to the noise inherent in the biomedical imaging process, varied cell appearance during collision, and abrupt change in cell motion patterns. This work presents a novel method to automatically track multiple cells undergoing collisions by modeling the collision states of cells and testing multiple hypotheses of their motion and ap¬pearance. The tracking results are demonstrated using in vivo intravital microscopy image sequences. We have found that: (1) the proposed method tracks 32% more cells than a previous approach without introducing any additional position error; (2) the improvement of the proposed method is emphasized when the duration of collision increases; and (3) given good detection results, the proposed method can correctly track 88% of the total number of cells.
Download or read book Advances in Intravital Microscopy written by Roberto Weigert and published by Springer. This book was released on 2014-11-12 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book entirely dedicated to Intravital Microscopy. It provides the reader with a broad overview of the main applications of Intravital Microscopy in various areas of the biomedical field. The book contains accurate descriptions of the state of the art methodologies used to image various organs at different level of resolution, ranging from whole tissue down to sub-cellular structures. Moreover, it is an extremely valuable guide to scientists that want to adopt this powerful technique and do not have experience with animal models and microscopy.
Download or read book Imaging from Cells to Animals In Vivo written by Margarida Barroso and published by CRC Press. This book was released on 2020-12-03 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.
Download or read book Visualizing Immunity written by Dorian McGavern and published by Springer Science & Business Media. This book was released on 2009-06-12 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Researchers have used a variety of techniques over the past century to gain fun- mental insights in the field of immunology and, as technology has advanced, so too has the ability of researchers to delve deeper into the biological mechanics of immunity. The immune system is exceedingly complex and must patrol the entire body to protect us from foreign invaders. This requires the immune system to be highly mobile and adaptable - able to respond to diverse microbial challenges while maintaining the ability to distinguish self from a foreign invader. This latter feature is of great importance because the immune system is equipped with toxic mediators, and a failure in self/non-self discrimination can result in serious diseases. Fortunately, in most cases, the immune system operates within the framework of its elegant design and protects us from diverse microbial challenges without initiating disease. Because the immune system is not confined to a single tissue, a comprehensive understanding of immunity requires that research be conducted at the molecular, cellular, and systems level. Immune cells often find customized solutions to h- dling microbial insults that depend on the tissue(s) in which the pathogen is found.
Download or read book Intravital Imaging of Dynamic Bone and Immune Systems written by Masaru Ishii and published by Humana. This book was released on 2019-06-06 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own.
Download or read book Detection of Rolling Leukocytes from Intravital Microscopy Images written by Gang Dong and published by . This book was released on 2006 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Intravital Imaging of Dynamic Behaviors of Leukocytes in UVB induced Skin Inflammation written by Ran Lu and published by . This book was released on 2013 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ultraviolet B (UVB) irradiation diminishes surface densities of Langerhans cells (LCs) in the epidermis of the skin. Other leukocytes, such as neutrophils and macrophages, are recruited to the irradiated sites due to chemo-attracting factors released from the altered microenvironment. However, there is little evidence directly demonstrating the behavior of LCs as well as other leukocytes in response to UVB radiation, and it is also unknown whether cellular interaction occurs during skin inflammation. In the present study, we developed a direct visualization system to observe the dynamic behaviors of both LCs and neutrophils responding to 3000J/m2 UVB in the skin. Using intravital confocal microscopy and genetically manipulated transgenic mice, we have found that neutrophils are recruited to inflammatory sites immediately after UVB irradiation. Skin-infiltrating neutrophils can interact with LCs upon activation and can last for several hours. Twenty-four hours after irradiation, LCs migrated to deeper skin layers, resulting in a 30% reduction of LC number in the epidermal compartment, whereas the number of recruited neutrophils increased over time. LCs completely disappeared from the epidermis 4 days after UVB treatment. Depletion of neutrophils by anti-Ly6G monoclonal antibody (mAb) administration did not affect LCs migration in response to the same UVB radiation, but rather promoted LC precursors' repopulation to the epidermis after UVB treatment. In addition, depletion of neutrophils greatly diminished the magnitude of UVB-induced ear swelling, suggesting that neutrophils are crucial for UVB induced skin inflammation. Not only do our data unveil the behavior and cell-cell interaction of LCs and neutrophils upon UVB irradiation, they also indicate for the first time that neutrophils play an important role in LC repopulation.
Download or read book Streptococcus pneumoniae written by Federico Iovino and published by Humana Press. This book was released on 2019-04-01 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field.
Download or read book Inflammation in the CNS Advancing the Field Using Intravital Imaging written by Saparna Pai and published by Frontiers Media SA. This book was released on 2017-12-20 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: Inflammation of the CNS can have devastating, long-lived, and in some cases fatal consequences for patients. The stimuli that can induce CNS inflammation are diverse, and include infectious agents, autoimmune responses against CNS-expressed antigens, and sterile inflammation following ischemia or traumatic injury. In these conditions, cells of the immune system play central roles in promulgation and resolution of the inflammatory response. However, the immunological mechanisms at work in these diverse responses differ according to the nature of the response. Our understanding of the actions of immune cells in the CNS has been restricted by the difficulty in visualising leukocytes as they undergo recruitment from the cerebral microvasculature and following their entry into the CNS parenchyma. However, advances in in vivo microscopy over the last 10-15 years have overcome many of these difficulties, and studies using these forms of microscopy have revealed a wealth of new information regarding the cellular and molecular mechanisms of CNS inflammation. This Research Topic brings together state of the art reviews examining the use of in vivo imaging in investigating inflammation and leukocyte behaviour in the CNS. Papers in this Research Topic describe how in vivo microscopy has increased our understanding of the actions of immune cells in the inflamed CNS, following various stimuli including autoimmunity, infection and sterile inflammation.
Download or read book Make Life Visible written by Yoshiaki Toyama and published by Springer Nature. This book was released on 2019-10-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.
Download or read book Correlative Imaging written by Paul Verkade and published by John Wiley & Sons. This book was released on 2019-11-04 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Brings a fresh point of view to the current state of correlative imaging and the future of the field This book provides contributions from international experts on correlative imaging, describing their vision of future developments in the field based on where it is today. Starting with a brief historical overview of how the field evolved, it presents the latest developments in microscopy that facilitate the correlative workflow. It also discusses the need for an ideal correlative probe, applications in proteomic and elemental analysis, interpretation methods, and how correlative imaging can incorporate force microscopy, soft x-ray tomography, and volume electron microscopy techniques. Work on placing individual molecules within cells is also featured. Correlative Imaging: Focusing on the Future offers in-depth chapters on: correlative imaging from an LM perspective; the importance of sample processing for correlative imaging; correlative light and volume EM; correlation with scanning probe microscopies; and integrated microscopy. It looks at: cryo-correlative microscopy; correlative cryo soft X-ray imaging; and array tomography. Hydrated-state correlative imaging in vacuo, correlating data from different imaging modalities, and big data in correlative imaging are also considered. Brings a fresh view to one of the hottest topics within the imaging community: the correlative imaging field Discusses current research and offers expert thoughts on the field’s future developments Presented by internationally-recognized editors and contributors with extensive experience in research and applications Of interest to scientists working in the fields of imaging, structural biology, cell biology, developmental biology, neurobiology, cancer biology, infection and immunity, biomaterials and biomedicine Part of the Wiley–Royal Microscopical Society series Correlative Imaging: Focusing on the Future will appeal to those working in the expanding field of the biosciences, correlative microscopy and related microscopic areas. It will also benefit graduate students working in microscopy, as well as anyone working in the microscopy imaging field in biomedical research.
Download or read book Template Matching Techniques in Computer Vision written by Roberto Brunelli and published by John Wiley & Sons. This book was released on 2009-04-29 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: The detection and recognition of objects in images is a key research topic in the computer vision community. Within this area, face recognition and interpretation has attracted increasing attention owing to the possibility of unveiling human perception mechanisms, and for the development of practical biometric systems. This book and the accompanying website, focus on template matching, a subset of object recognition techniques of wide applicability, which has proved to be particularly effective for face recognition applications. Using examples from face processing tasks throughout the book to illustrate more general object recognition approaches, Roberto Brunelli: examines the basics of digital image formation, highlighting points critical to the task of template matching; presents basic and advanced template matching techniques, targeting grey-level images, shapes and point sets; discusses recent pattern classification paradigms from a template matching perspective; illustrates the development of a real face recognition system; explores the use of advanced computer graphics techniques in the development of computer vision algorithms. Template Matching Techniques in Computer Vision is primarily aimed at practitioners working on the development of systems for effective object recognition such as biometrics, robot navigation, multimedia retrieval and landmark detection. It is also of interest to graduate students undertaking studies in these areas.
Download or read book Mechanobiology written by Glen L. Niebur and published by Elsevier. This book was released on 2019-12-01 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mechanobiology: From Molecular Sensing to Disease will provide a review of the current state of understanding of mechanobiology and its role in health and disease. It covers: Current understanding of the main molecular pathways by which cells sense and respond to mechanical stimuli, A review of diseases that with known or purported mechanobiological underpinnings; The role of mechanobiology in tissue engineering and regenerative medicine; Experimental methods to capture mechanobiological phenomena; Computational models in mechanobiology. - Presents our current understanding of the main molecular pathways by which cells sense and respond to mechanical stimuli - Provides a review of diseases with known or purported mechanobiological underpinnings - Includes the role of mechanobiology in tissue engineering and regenerative medicine - Covers experimental methods to capture mechanobiological phenomena
Download or read book The Guide to Investigation of Mouse Pregnancy written by B. Anne Croy and published by Academic Press. This book was released on 2013-12-09 with total page 829 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epigenetic regulation, gene imprinting, and stem cells. Offering detailed and integrated information on how drugs, biologics, stress, and manipulations impact pregnancy in the mouse model, this reference highlights techniques used to analyze mouse pregnancy. Joining the ranks of much referenced mouse resources, The Guide to Investigation of Mouse Pregnancy is the only manual providing needed content on pregnancy in animal models for translational medicine and research. - Provides instruction on how to collect pre-clinical data on pregnancy in mouse models for eventual use in human applications - Describes the angiogenic tree the mother's uterus develops to support pregnancy and the monitoring of pregnancy-induced cardiovascular changes - Educates readers on placental cell lineages, decidual development including immune cells, epigenetic regulation, gene imprinting, stem cells, birth and lactation - Discusses how stress, environmental toxicants and other manipulations impact upon placental function and pregnancy success
Download or read book Optical Imaging in Human Disease and Biological Research written by Xunbin Wei and published by Springer Nature. This book was released on 2021-05-29 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book introduces readers to the basic principle of optical imaging technologies. Focusing on human disease diagnostics using optical imaging methods, it provides essential information for researchers in various fields and discusses the latest trends in optical imaging. In recent decades, there has been a huge increase in imaging technologies and their applications in human diseases diagnostics, including magnetic resonance imaging, x-ray computed tomography, and nuclear tomographic imaging. This book promotes further developments to extend optical imaging to a wider range of disease diagnostics. It is a valuable resource for researchers and students in the field of biomedical optics, as well as for clinicians.
Download or read book Video Microscopy written by and published by Academic Press. This book was released on 1998-04-10 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques.Practical laboratory guide for methods and technologies used with video microscopyComprehensive, easy-to-follow instructionsFebruary 1998, c. 334 pp.
