Download or read book Internal Energy Effects on the Solvation and Reactivity of Multiply Charged Biomolecules for Electrospray Ionization Mass Spectroscopy written by and published by . This book was released on 1992 with total page 3 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mild (capillary) interface conditions which do not completely desolvate the ions of proteins in electrospray ionization mass spectrometry (ESI-MS) may be required to probe the higher order structures and weak associations. For the small protein bovine ubiquitin, two ion distributions (unsolvated ions and unresolved solvated ions) were observed. The resolvable solvation for leucine-enkephalin with methanol and water shows that the use of countercurrent N2 flow at the capillary affects the solvation observed. 2 figs. (DLC).
Download or read book Internal Energy Effects on the Solvation and Reactivity of Multiply Charged Biomolecules for Electrospray Ionization Mass Spectroscopy Bovine Ubiquitin written by and published by . This book was released on 1992 with total page 3 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mild (capillary) interface conditions which do not completely desolvate the ions of proteins in electrospray ionization mass spectrometry (ESI-MS) may be required to probe the higher order structures and weak associations. For the small protein bovine ubiquitin, two ion distributions (unsolvated ions and unresolved solvated ions) were observed. The resolvable solvation for leucine-enkephalin with methanol and water shows that the use of countercurrent N2 flow at the capillary affects the solvation observed. 2 figs. (DLC).
Download or read book Energy Research Abstracts written by and published by . This book was released on 1993 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proceedings of the ASMS Conference on Mass Spectrometry and Allied Topics written by and published by . This book was released on 1992 with total page 2284 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Government Reports Annual Index written by and published by . This book was released on 1993 with total page 1452 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ionization Methods in Organic Mass Spectrometry written by Alison E Ashcroft and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ionization Methods in Organic Mass Spectrometry is a basic practical guide for scientists of all disciplines who wish to analyse samples by organic mass spectrometry. Concentrating on instrumental operation, this book gives step-by-step instructions on how to set up, and how to achieve the best results, using a range of ionization methods, including atmospheric pressure chemical ionization, electrospray ionization and matrix assisted laser desorption ionization. Ionization Methods in Organic Mass Spectrometry will enable a beginner, or practitioner with limited experience, to choose the most appropriate ionization technique in application areas such as biomolecules, drugs and metabolites, pesticides, polymers and many other organic compounds. It will be a valuable practical guide for technicians, graduates, students or researchers - or indeed anyone new to practical organic mass spectrometry.
Download or read book Structural and Energetic Studies of Hydrated Gas phase Ions and Biomolecules Using Electrospray Ionization Mass Spectrometry written by Sandra Enid Rodriguez-Cruz and published by . This book was released on 1999 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Electrospray Ionization Mass Spectrometry for Analysis of Water Soluble and Membrane Proteins and Educational Protocols for an Analytical Chemistry Class written by Wonhyeuk Jung and published by . This book was released on 2021 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: Native mass spectrometry (MS) is a branch of MS analysis in which the structure of the target analytes of interest are kept intact and remaining in their "native" functional structure (as much as possible). This approach was made possible by the development of electrospray ionization (ESI), a soft ionization technique that does not fragment the target analyte during the ionization process while inducing multiple charging. The multiply charged biomolecules, in turn, can be subjected to fragmentation via collisional activation with a non-reactive gas such as nitrogen. This approach of combining native MS with fragmentation-based analysis, termed native topdown MS analysis, can be applied to large biomolecules such as membrane proteins to gain structural insights. Membrane proteins present unique challenges to conventional high-resolution structural techniques due to their hydrophobic nature. However, they are responsible for various physiological phenomena and account for 60% of known druggable targets in the cell. Thus, there is a need for an approach that can overcome issues with membrane protein analysis while complementing other biophysical techniques used to probe protein structure. Here, how native top-down MS can play this role is presented. The effects of non-ionic saccharide-based detergents, a commonly used class of detergents for membrane protein solubilization, on the resulting charge states of soluble proteins is investigated to gain insights into the mechanism of ESI. The MS-fragmentation patterns from collisionally activated dissociation of membrane proteins and membrane protein-lipid complexes are compared. How new insights into the lipid binding sites can be gained by detecting lipid-bound MS-fragments is presented. The result of the study indicates that native top-down MS analysis can provide unique structural insights for membrane proteins and their non-covalent interactions. When the analytical goal is to investigate the atomic composition of the target analyte, an ionization approach in which the sample is fully atomized before MS analysis is preferred instead. Inductively coupled plasma ionization, which atomizes and ionizes the sample via a plasma, can be coupled with MS analysis (ICP-MS) to quantify heavy metal contamination in complex samples. A protocol for ICP-MS analysis of commercial fish products for mercury contamination detection developed to aid an analytical chemistry class for instruction of undergraduate chemistry students is presented.
Download or read book Government Reports Announcements Index written by and published by . This book was released on 1993-02 with total page 1446 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrospray Ionization Mass Spectrometry written by Richard B. Cole and published by Wiley-Interscience. This book was released on 1997-05-06 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive, up-to-date coverage of a revolutionary technique Electrospray ionization mass spectrometry (ESI-MS) has completely changed the way physicists, chemists, and biologists view the study of large molecules. The technique derives detailed information about molecular weights and structures from extremely small sample quantities. ESI-MS can create highly charged forms of very high molecular weight compounds, it is naturally compatible with many types of separation techniques, and it allows noncovalent interactions between molecules in solution to be preserved in the gas phase. But many researchers may not use the technique to its full potential because they are unfamiliar with the different perspectives of its underlying processes, the varied approaches to implementation, and the wide-ranging utility of the technique. In this book, Richard B. Cole and an assemblage of leading researchers present a single-volume compilation of different approaches to the understanding and exploitation of ESI-MS. This comprehensive guide: * Examines the physical and chemical aspects of the electrospray process and describes the events involved in ion formation as well as the electro-chemical phenomena that are central to charged droplet formation during the process * Explores the coupling of electrospray ionization to various mass spectrometers, including quadrupole, magnetic, time-of-flight, quadrupole ion trap, and Fourier transform ion cyclotron resonance instruments * Describes recent progress in interfacing ESI with solution-based separation techniques, including liquid chromatography and capillary electrophoresis * Charts the rapid development of ESI applications and categorizes them by compound type: peptides and proteins, nucleic acids and their constituents, carbohydrates and lipids, small molecules related to pharmacology and drug metabolism, and organometallics and inorganic compounds Electrospray Ionization Mass Spectrometry is the indispensable handbook and reference for anyone who wishes to understand, implement, or apply this technique, including researchers in chemistry, metallochemistry, biochemistry, biology, pharmacology, and physics.
Download or read book Faculties Publications and Doctoral Theses in Chemistry and Chemical Engineering at United States Universities written by American Chemical Society. Committee on Professional Training and published by . This book was released on 1991 with total page 1646 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solvent Effects and Chemical Reactivity written by Orlando Tapia and published by Springer Science & Business Media. This book was released on 2003-07-31 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an up-to-date view of theories, practical methods and applications of solvent effects and chemical reactivity in condensed phases. Subjects treated include continuum solvation models, the theoretical basis for the treatment of solvent effects in density functional theory, Monte Carlo simulations of chemical reactions in solution, DFT molecular dynamics simulations, crossing the transition state in solution, valence bond multi-state approach to chemical reactions in solution, quantum theory of solvent effects and chemical reactions. The approaches taken as well as the resulting findings are discussed in detail, thus covering a large part of the methodology currently used in this field. Audience: This volume will be useful to graduate students in chemistry, physical chemistry and biochemistry, to research workers with a background in quantum chemistry and quantum mechanics, to pure and applied quantum chemists, and to industrial molecular modellers.
Download or read book Electrospray and MALDI Mass Spectrometry written by Richard B. Cole and published by John Wiley & Sons. This book was released on 2011-09-26 with total page 900 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. In particular, this Second Edition highlights how advances in electrospray and MALDI mass spectrometry are supporting important discoveries in new and emerging fields such as proteomics and metabolomics as well as in traditional areas of chemistry and physics research. Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is divided into five parts: Part A, Fundamentals of ES, explains the fundamental phenomena underlying the electrospray process, including selectivity in ionization and inherent electrochemistry, and concludes with a chapter offering a comparative inventory of source hardware Part B, Fundamentals of MALDI, confronts ionization mechanisms, instrument development, and matrix selection, and includes a final chapter that explores the special application of MALDI to obtain two-dimensional images of spatial distributions of compounds on surfaces Part C, ES and MALDI Coupling to Mass Spectrometry Instrumentation, examines the coupling of these ionization techniques to various mass analyzers, including quadrupole ion trap, time-of-flight, Fourier transform ion cyclotron resonance, and ion mobility mass spectrometers Part D, Practical Aspects of ES and MALDI, investigates analytical issues including quantification, charge-state distributions, noncovalent interactions in solution that are preserved as gas-phase ions, and various means of ion excitation in preparation for tandem mass spectrometry, and offers a guide to the interpretation of even-electron mass spectra Part E, Biological Applications of ES and MALDI, examines the role of mass spectrometry in such areas as peptide and protein characterization, carbohydrate analysis, lipid analysis, and drug discovery Written by a team of leading experts, the book not only provides a critical review of the literature, but also presents key concepts in tutorial fashion to help readers take full advantage of the latest technological breakthroughs and applications. As a result, Electrospray and MALDI Mass Spectrometry will help researchers fully leverage the power of electrospray and MALDI mass spectrometry. The judicious compartmentalization of chapters, and the pedagogic presentation style throughout, render the book highly suitable for use as a text for graduate-level courses in advanced mass spectrometry.
Download or read book Solvation Dynamics written by Chang Q Sun and published by Springer. This book was released on 2019-07-03 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the latest advances and outlines future trends in aqueous solvation studies from the perspective of hydrogen bond transition by charge injection, which reconciles the solvation dynamics, molecular nonbond interactions, and the extraordinary functionalities of various solutes on the solution bond network and properties. Focus is given on ionic and dipolar electrostatic polarization, O:H nonbond interaction, anti-HB and super-HB repulsion, and solute-solute interactions. Its target audience includes researchers, scientists, and engineers in chemistry, physics, surface and interface science, materials science and engineering.
Download or read book Methods and Mechanisms for Producing Ions from Large Molecules written by K.G. Standing and published by Springer. This book was released on 1991 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: A NATO Advanced Research Workshop on Methods and Mechanisms for Producing Ions from Large Molecules was held at Minaki Lodge, Minaki, Ontario, Canada, from 24 to 28 June 1990. The workshop was hosted by the time-of-flight group of the Department of Physics at the University of Manitoba, and was attended by 64 invited participants from around the world. Twenty-nine invited talks were given and 19 papers were presented as posters. Of the 48 contributions, 38 are included in these proceedings. The conference was organized to study the rapidly changing field of mass spectrometry of biomolecules. Particle-induced desorption (especially with MeV particles) has been the most effective method of producing molecular ions from biomolecules. An important part of the workshop was devoted to recent developments in this field, particularly to progress in understanding the fundamentals of the desorption process. In this respect, the meeting was similar to previous conferences in Marburg, FRG (1978); Paris, F (1980); Uppsala, S (1981); College Station, USA (1983,1984); Wangerooge, FRG (1986); Orsay, F (1988); Spiekeroog, FRG (1989); and to the IFOS series of meetings at Munster, FRG (1981,1983,1985,1987) and L6vAnger, S (1989). As in the most recent of these meetings, there was some emphasis on new developments, particularly cluster bombardment. A departure from the concentration on particle bombardment processes at this conference was inspired by the dramatic results obtained with two new methods for producing molecular ions from large molecules: matrix-assisted laser desorption and electrospray.
Download or read book Internal Energy Effects in Tandem Mass Spectrometry written by Maria del Pilar Ospina and published by . This book was released on 1998 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry written by A. Peter Snyder and published by . This book was released on 1996 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents new ways of creating ions of polar and labile biopolymeric species with remarkable ease and efficiency. Introduces better characterization of proteins and other biological molecules. Presents major fundamental issues in such areas as immunology, biochemistry, and natural toxins. Demonstrates methods used to resolve the data from various biological process investigations into useful information. Provides an application-driven approach, presenting valuable insights into the experiments in the volume.
