Download or read book Interactions Between Nanospheres and Lipid Membranes written by Sudhir Upreti and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Potential applications of nanoparticles in various field including medicine and industries demand careful investigations of the interaction between nanoparticles and the plasma membrane and the resulting effect this has on the structural, mechanical and thermal properties of the plasma membrane. Furthermore, understanding the interactions between nanoparticles and bio membranes will allow us to design nanoparticles for optimal transmembrane transport. Using large-scaleLangevin molecular dynamics simulations, we systematically investigated the adsorption of spherical nanoparticles on lipid membranes, their effect on the morphological changes of the membrane, and the clustering of the nanoparticles. Our study is performed with changing the parameters such as the diameter of the nanoparticle, the strength of their interaction with the biomembrane and the surface coverage of the nanoparticles. We observed endocytosis in the membrane for single spherical nanoparticle at high interaction potential and small radius and relatively low interaction potential and high radius. Our results also indicate that for high nanoparticle density and for strong adsorption interactions the nanoparticles can lead to membrane lysis. However, for moderate adsorption interaction, the nanoparticles form long-lived linear clusters that are due to an effective curvature-driven attraction between the nanoparticles.

Download or read book Colloidal Silica written by Horacio E. Bergna and published by CRC Press. This book was released on 2005-12-19 with total page 926 pages. Available in PDF, EPUB and Kindle. Book excerpt: In spite of the apparent simplicity of silica's composition and structure, scientists are still investigating fundamental questions regarding the formation, constitution, and behavior of colloidal silica systems. Colloidal Silica: Fundamentals and Applications introduces new information on colloid science related to silica chemistry as well

Download or read book Interaction of Amphiphilic Nanoparticles with Structurally Perturbed Lipid Membranes written by Alexander W. Derry and published by . This book was released on 2018 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the interactions between nanoparticles and lipid membranes is important for applications such as drug delivery and membrane-protein mimetics. Perturbations such as area asymmetry and lateral tension affect these interactions by inducing various structural changes to the membranes. We use molecular dynamics simulations to demonstrate that the introduction of area asymmetry to a bilayer membrane significantly decreases the insertion latency of amphiphilic gold nanoparticles into both the densely and sparsely packed leaflets. We further demonstrate using transition state analysis that the dominant mechanisms for insertion into the dense and sparse leaflets are lipid desorption and lipid tail protrusions, respectively. These findings are supported by potential of mean force calculations showing that the energy barrier to protrusion is lower in the sparse leaflet, while that of desorption is lower in the dense leaflet. We also demonstrate that the structural characteristics of the bilayer when subject to lateral tension are similar to that observed in membranes with area asymmetry, suggesting a similar reduction in insertion latency. Further, we observe that lateral tension also increases the likelihood of nanoparticle ligands flipping across the bilayer, which is necessary for the nanoparticle adopting a stable symmetric configuration in the membrane.

Download or read book Computer Simulations of Nanorods Interaction With Lipid Membrane written by Niraj Shrestha and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Analyzing the interaction between the lipid membranes and nanoparticles helps to determine beneficial and harmful effects of nanoparticles on cells. Since the point of entry of cells is their plasma membrane, interactions between nanorods and lipid membranes were studied using molecular dynamics simulations. The effect of nanorods on membrane morphology was investigated systematically as a function of nanorods interaction with the lipid membrane, their surface coverage and number surface density. Our results indicate that when adsorbed on the membrane, the nanorods may cluster into chains and may lead to strong membrane deformations due to curvature driven interaction between nanorods. At particular nanorod-lipid interaction, single nanorod internalizes into the membrane via endocytosis. This happens to minimize the energy cost due to high bending of the membrane. At high nanorods' density and large nanorods' size it was found that the nanorods lead to membrane lysis which can be attributed to high surface coverage of nanorods.

Download or read book Surface Chemistry of Nanobiomaterials written by Alexandru Grumezescu and published by William Andrew. This book was released on 2016-02-03 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface Chemistry of Nanobiomaterials brings together the most recent findings regarding the surface modification of currently used nanomaterials, which is a field that has become increasingly important during the last decade. This book enables the results of current research to reach those who wish to use this knowledge in an applied setting. Leading researchers from around the world present various types of nanobiomaterials, such as quantum dots (QDs), carbon nanotubes, silver nanoparticles, copper oxide, zinc oxide, magnesium oxide, magnetite, hydroxyapatite and graphene, and discuss their related functionalization strategies. This book will be of interest to postdoctoral researchers, professors and students engaged in the fields of materials science, biotechnology and applied chemistry. It will also be highly valuable to those working in industry, including pharmaceutics and biotechnology companies, medical researchers, biomedical engineers and advanced clinicians. - An up-to-date and highly structured reference source for researchers, practitioners and students working in biomedical, biotechnological and engineering fields - A valuable guide to recent scientific developments, covering major and emerging applications of nanomaterials in the biomedical field - Proposes novel opportunities and ideas for developing or improving technologies in nanomedicine and nanobiology

Download or read book Handbook of Lipid Membranes written by Cyrus R. Safinya and published by CRC Press. This book was released on 2021-09-14 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook provides a unique overview of lipid membrane fundamentals and applications. The fascinating world of lipids that harbor and govern so many biological functionalities are discussed within the context of membrane structures, interactions, and shape evolution. Beyond the fundamentals in lipid science, this handbook focuses on how scientists are building bioinspired biomimetic systems for applications in medicine, cosmetics, and nanotechnology. Key Features: Includes experimental and theoretical overviews on the role of lipids, with or without associated biomolecules, as structural components imparting distinct membrane shapes and intermembrane interactions Covers the mechanisms of lipid-membrane curvature, by peptide and protein binding, and the roles of signalling lipids and the cytoskeleton in plasma membrane shape evolution Covers advanced X-ray and force measurement techniques Discusses applications in biomedicine, cosmetics, and nanotechnology, including lipid vectors in nucleic acid, drug delivery in dermal applications, and lipid-based sensors and artificial biointerfaces Covers artificial membranes from block copolymers, synthetic copolypeptides, and recombinant proteins Includes an exciting section that explores the role of lipids in the origin of life in hydrothermal conditions This book is a highly informative companion for professionals in biophysics, biochemistry, physical chemistry, and material and pharmaceutical sciences and bioengineering.

Download or read book Modelling of Interactions Between Lipid Bilayers and Nanoparticles of Various Degrees of Hydrophobicity written by Chanfei Su and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advances in Biomembranes and Lipid Self Assembly written by and published by Elsevier. This book was released on 2023-12-01 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biomembranes and Lipid Self-Assembly, Volume 38 in this updated series, highlights new advances in the field, with this new volume presenting interesting chapters on Interaction of inorganic debris particles with cells, Interactions between biomembrane embedded nanoparticles mediated by lipid bilayer, Topological defect driven nanoparticle assemblies, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Advances in Biomembranes and Lipid Self-Assembly - Updated release includes the latest information on the Interaction of inorganic debris particles with cells, Interactions between biomembrane embedded nanoparticles mediated by lipid bilayer, and more

Download or read book Protein Nanoparticle Interactions written by Masoud Rahman and published by Springer Science & Business Media. This book was released on 2013-06-24 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the fabrication of nanomaterials and exploration of their properties have attracted the attention of various scientific disciplines such as biology, physics, chemistry, and engineering. Although nanoparticulate systems are of significant interest in various scientific and technological areas, there is little known about the safety of these nanoscale objects. It has now been established that the surfaces of nanoparticles are immediately covered by biomolecules (e.g. proteins, ions, and enzymes) upon their entrance into a biological medium. This interaction with the biological medium modulates the surface of the nanoparticles, conferring a “biological identity” to their surfaces (referred to as a “corona”), which determines the subsequent cellular/tissue responses. The new interface between the nanoparticles and the biological medium/proteins, called “bio-nano interface,” has been very rarely studied in detail to date, though the interest in this topic is rapidly growing. In this book, the importance of the physiochemical characteristics of nanoparticles for the properties of the protein corona is discussed in detail, followed by comprehensive descriptions of the methods for assessing the protein-nanoparticle interactions. The advantages and limitations of available corona evaluation methods (e.g. spectroscopy methods, mass spectrometry, nuclear magnetic resonance, electron microscopy, X-ray crystallography, and differential centrifugal sedimentation) are examined in detail, followed by a discussion of the possibilities for enhancing the current methods and a call for new techniques. Moreover, the advantages and disadvantages of protein-nanoparticle interaction phenomena are explored and discussed, with a focus on the biological impacts.

Download or read book Membrane Organization and Dynamics written by Amitabha Chattopadhyay and published by Springer. This book was released on 2017-12-06 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume brings together information on membrane organization and dynamics from a variety of spectroscopic, microscopic and simulation approaches, spanning a broad range of time scales. The implication of such dynamic information on membrane function in health and disease is a topic of contemporary interest. The chapters cover various aspects of membrane lipid and protein dynamics, explored using a battery of experimental and theoretical approaches. The synthesis of information and knowledge gained by utilizing multiple approaches will provide the reader with a comprehensive understanding of the underlying membrane dynamics and function, which will help to develop robust dynamic models for the understanding of membrane function in healthy and diseased states. In the last few years, crystal structures of an impressive number of membrane proteins have been reported, thanks to tremendous advances in membrane protein crystallization techniques. Some of these recently solved structures belong to the G protein-coupled receptor (GPCR) family, which are particularly difficult to crystallize due to their intrinsic flexibility. Nonetheless, these static structures do not provide the necessary information to understand the function of membrane proteins in the complex membrane milieu. This volume will address the dynamic nature of membrane proteins within the membrane and will provide the reader with an up-to date overview of the theory and practical approaches that can be used. This volume will be invaluable to researchers working in a wide range of scientific areas, from biochemistry and molecular biology to biophysics and protein science. Students of these fields will also find this volume very useful. This book will also be of great use to those who are interested in the dynamic nature of biological processes.

Download or read book Liposomes Lipid Bilayers and Model Membranes written by Georg Pabst and published by CRC Press. This book was released on 2014-03-04 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a result of their unique physical properties, biological membrane mimetics, such as liposomes, are used in a broad range of scientific and technological applications. Liposomes, Lipid Bilayers and Model Membranes: From Basic Research to Application describes state-of-the-art research and future directions in the field of membranes, which has evolved from basic studies of the physicochemical properties of amphiphiles to their application in industry and medicine. Written by leading researchers in their fields, this book describes basic and applied research, and serves as a useful reference for both the novice and the expert. Part one covers a range of basic research topics, from theory and computational simulations to some of the most up-to-date experimental research. Topics discussed include soft matter physics of membranes, nonlamellar phases, extraction of molecules by amphiphiles, lipid models for membrane rafts, membrane dynamics, nanodiscs, microemulsions, active membranes, as well as interactions of bilayers with drugs or DNA to treat disease or for gene transfer, respectively. Part two of the book focuses on technological applications of amphiphiles, such as liposome-based nanoparticles for drug delivery, formulation of liposomes for prolonged in vivo circulation and functionalization for medical purposes, novel drug delivery systems for increased drug loading, and the use of tethered membranes for bio-sensing applications. Chapters also describe the use of liposomes in textile dyeing and how lipidic nanoparticles are used by the food industry.

Download or read book Interactions of Charged and Superparamagnetic Nanoparticles with Lipid Model Membranes written by Janez Ivan Pavlič and published by . This book was released on 2010 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Analytical Models to Understand Interactions at the Nano bio Interface written by Christian A. Reardon-Lochbaum and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomaterials are synthesized for use in a wide variety of consumer and industrial products, which will inadvertently lead to nanomaterial exposure to biological systems; additionally, nanomaterials are being explored for application in both the medical and agricultural fields, which constitutes intentional nanomaterial exposure to biological systems. With the rapid expansion in the quantity and variety of nanomaterials being synthesized, it has become a huge undertaking to characterize the interactions at the nano-bio interface. Here, I present a series of studies that work towards developing and utilizing model systems to characterize interactions between nanomaterials and cellular membranes or biological fluids. This work targets two model membrane systems-the supported lipid bilayer and unilamellar vesicles-and one biological fluid model system-plant xylem fluid. Membranes make up one of the cells most robust defense systems from invasive material. They provide both a physical and chemical barrier between the interior and exterior of the cell. Cellular membranes are complex, containing multitudes of different lipid varieties that assemble into surface features and incorporate membrane proteins that actively perform tasks while contained within the bilayer. Due to this complexity, interactions between native cell membranes and nanoparticles are difficult to characterize when performing whole cell exposure studies. Lipid bilayer model systems simplify the cell membrane down to a handful of molecular components so that fundamental mechanisms of interaction between nanoparticles and lipids can be studied. The work presented here has focused on the simplest variety of model lipid bilayer, the single component lipid bilayer, to allow for rigorous characterization of molecular interactions with nanomaterials. We used the first model system highlighted here, supported lipid bilayers, to understand the interactions between ligand coated gold nanoparticles and cellular membranes. We exposed supported lipid bilayers to 2 nm gold nanoparticles functionalized with ligands of systematically increasing lipophilicity. We found that increasing lipophilicity of ligands increased the maximum mass density of nanoparticle adsorption and the rate at which nanoparticles converted to the quasi-irreversibly adsorbed state. The quasi-irreversibly bound state was linked to ligand insertion, which we define by the ability of a ligand to form hydrophobic contact with lipid tail groups. An issue with previous applications of the supported lipid bilayer system is that it under predicts the interaction of anionic nanoparticles with the membrane when compared to both computational and cellular exposure models. We found that interactions between anionic nanoparticles and supported lipid bilayers are impacted by the composition of the substrate upon which the bilayer is built. Specifically, we find that SiO2-a widely used substrate-hindered the adsorption of anionic gold nanoparticles to the bilayer as compared to a Au substrate. This change is attributed to both the increased attractive van der Waals forces and decreased repulsive Coulombic forces of nanoparticles interacting with bilayers formed on Au vs SiO2 substrates, respectively. A second model membrane system, unilamellar vesicles, has been used to monitor nanomaterial induced disruption or remodeling of lipid bilayers; however, a difficulty when using this model system is the inability to rigorously track the nanomaterial with respect to a disruption or remodeling event, due to the small size dimensions of the nanoparticle. We work to develop a method of tracking both fluorescent vesicles and nanomaterials simultaneously with convex lens induced confinement. The final set of studies presented here utilizes a third model biological system: plant xylem fluid. Nanomaterial based approaches to suppressing plant disease and increasing crop yields are being explored; however, little is known about molecular level interactions between the nanomaterials proposed in this research and the plants. We conducted a set of measurements to understand how copper oxide nanoparticles, a nanomaterial that has shown promise as a micronutrient delivery to plants, interact with the biomolecules found in xylem fluid. We found that proteins from the xylem fluid associate with the nanoparticles, a phenomenon known as biomolecular corona formation. Additionally, we found that protein adsorption is selective to a subset of proteins found within the xylem fluid and the corona evolves over time.0́3

Download or read book Biomimetic Lipid Membranes Fundamentals Applications and Commercialization written by Fatma N. Kök and published by Springer. This book was released on 2019-04-16 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book compiles the fundamentals, applications and viable product strategies of biomimetic lipid membranes into a single, comprehensive source. It broadens its perspective to interdisciplinary realms incorporating medicine, biology, physics, chemistry, materials science, as well as engineering and pharmacy at large. The book guides readers from membrane structure and models to biophysical chemistry and functionalization of membrane surfaces. It then takes the reader through a myriad of surface-sensitive techniques before delving into cutting-edge applications that could help inspire new research directions. With more than half the world's drugs and various toxins targeting these crucial structures, the book addresses a topic of major importance in the field of medicine, particularly biosensor design, diagnostic tool development, vaccine formulation, micro/nano-array systems, and drug screening/development. Provides fundamental knowledge on biomimetic lipid membranes; Addresses some of biomimetic membrane types, preparation methods, properties and characterization techniques; Explains state-of-art technological developments that incorporate microfluidic systems, array technologies, lab-on-a-chip-tools, biosensing, and bioprinting techniques; Describes the integration of biomimetic membranes with current top-notch tools and platforms; Examines applications in medicine, pharmaceutical industry, and environmental monitoring.

Download or read book Modeling of Interactions Between Nanoparticles and Cell Membranes written by Young-Min Ban and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The hydrophobic interaction between the particles and membrane core induces the strong coupling between the nanoparticle motion and membrane deformation. It is observed that the considered nanoparticles affect several physical properties of the membrane. The nanoparticles embedded into the membrane interior lead to the membrane softening, which becomes more significant with increase in CNT length and concentration. The lateral pressure profile and membrane energy in the membrane containing the nanoparticles exhibit localized perturbation around the nanoparticle. The nanoparticles are not likely to affect membrane protein function by the weak perturbation of the internal stress in the membrane. Due to the short-ranged interactions between the nanoparticles, the nanoparticles would not form aggregates inside membranes. The effect of lipid peroxidation on cell membrane deformation is assessed. The peroxidized lipids introduce a perturbation to the internal structure of the membrane leading to higher amplitude of the membrane fluctuations. Higher concentration of the peroxidized lipids induces more significant perturbation. Cumulative effects of lipid peroxidation caused by nanoparticles are examined for the first time. The considered amphiphilic particle appears to reduce the perturbation of the membrane structure at its equilibrium position inside the peroxidized membrane. This suggests a possibility of antioxidant effect of the nanoparticle.

Download or read book Gold Nanoparticles in Biomedical Applications written by Lev Dykman and published by CRC Press. This book was released on 2017-12-12 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses fabrication of functionalized gold nanoparticles (GNPs) and multifunctional nanocomposites, their optical properties, and applications in biological studies. This is the very first book of its kind to comprehensively discuss published data on in vitro and in vivo biodistribution, toxicity, and uptake of GNP by mammalian cells providing a systematization of data over the GNP types and parameters, their surface functionalization, animal and cell models. As distinct from other related books, Gold Nanoparticles in Biomedical Applications discusses the immunological properties of GNPs and summarizes their applications as an antigen carrier and adjuvant in immunization for the preparation of antibodies in vivo. Although the potential of GNPs in nanobiotechnology has been recognized for the past decade, new insights into the unique properties of multifunctional nanostructures have recently emerged. With these developments in mind, this book unites ground breaking experimental data with a discussion of hybrid nanoparticle systems that combine different nanomaterials to create multifunctional structures. These novel hybrids constitute the material basis of theranostics, bringing together the advanced properties of functionalized GNPs and composites into a single multifunctional nanostructure with simultaneous diagnostic and therapeutic functions. Such nanohybrids can be physically and chemically tailored for a particular organ, disease, and patient thus making personalized medicine available.

Download or read book Peptide Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction